(Circular) (Six-base) MAPSORT of: pFN21AB7327.seq Check: 7312 from: 1 to: 7219 pFN21AB7327 7219 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5029 278 Size: 53 83 186 936 3493 2468 Fragments arranged by size: 3493 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2183 2203 2846 3523 4562 1937 Size: 246 20 643 677 1039 4594 Fragments arranged by size: 4594 1039 677 643 246 20 Acc65I G'GTAC_C Cuts at: 2196 2679 4540 2196 Size: 483 1861 4875 Fragments arranged by size: 4875 1861 483 AclI AA'CG_TT Cuts at: 1563 5346 5719 1563 Size: 3783 373 3063 Fragments arranged by size: 3783 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2507 3297 3380 3687 3698 3913 4094 4425 Size: 875 790 83 307 11 215 181 331 Cuts at: 4425 4512 5282 6666 6934 1632 Size: 87 770 1384 268 1917 Fragments arranged by size: 1917 1384 875 790 770 331 307 268 215 181 87 83 11 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7022 AflIII A'CryG_T Cuts at: 1203 3474 6118 7095 1203 Size: 2271 2644 977 1327 Fragments arranged by size: 2644 2271 1327 977 AgeI A'CCGG_T Cuts at: 6050 6050 Size: 7219 AhdI GACnn_n'nnGTC Cuts at: 5948 5948 Size: 7219 AleI CACnn'nnGTG Cuts at: 1166 2075 1166 Size: 909 6310 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7219 AlwNI CAG_nnn'CTG Cuts at: 4962 6534 4962 Size: 1572 5647 ApaLI G'TGCA_C Cuts at: 5276 6432 5276 Size: 1156 6063 ApoI r'AATT_y Cuts at: 1493 2190 2243 2344 2442 2591 2870 3764 3793 Size: 697 53 101 98 149 279 894 29 Cuts at: 3793 4294 4486 4528 4754 4906 7015 1493 Size: 501 192 42 226 152 2109 1697 Fragments arranged by size: 2109 1697 894 697 501 279 226 192 152 149 101 98 53 42 29 AseI AT'TA_AT Cuts at: 160 1032 2730 4047 4316 5773 160 Size: 872 1698 1317 269 1457 1606 Fragments arranged by size: 1698 1606 1457 1317 872 269 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7219 AvaI C'yCGr_G Cuts at: 1870 1943 4544 1870 Size: 73 2601 4545 Fragments arranged by size: 4545 2601 73 BamHI G'GATC_C Cuts at: 3482 3994 4549 4916 3482 Size: 512 555 367 5785 Fragments arranged by size: 5785 555 512 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2196 2679 4540 5995 618 Size: 325 217 627 409 483 1861 1455 1842 Fragments arranged by size: 1861 1842 1455 627 483 409 325 217 BanII G_rGCy'C Cuts at: 1391 4538 1391 Size: 3147 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3361 928 Size: 971 1462 4786 Fragments arranged by size: 4786 1462 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5410 5444 1903 Size: 34 50 34 3389 34 3678 Fragments arranged by size: 3678 3389 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5111 6321 5111 Size: 1210 6009 BclI T'GATC_A Cuts at: 1548 3597 4239 4426 1548 Size: 2049 642 187 4341 Fragments arranged by size: 4341 2049 642 187 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5830 136 Size: 107 122 71 631 605 4158 1525 Fragments arranged by size: 4158 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2518 3435 4390 7214 2518 Size: 917 955 2824 2523 Fragments arranged by size: 2824 2523 955 917 BlpI GC'TnA_GC Cuts at: 4636 4636 Size: 7219 Bme1580I G_kGCm'C Cuts at: 1790 5280 6436 1790 Size: 3490 1156 2573 Fragments arranged by size: 3490 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2997 1318 Size: 1679 5540 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2600 4093 4950 Size: 416 53 83 399 24 1183 1493 857 Cuts at: 4950 5908 442 Size: 958 1753 Fragments arranged by size: 1753 1493 1183 958 857 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7219 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2031 2682 5879 1239 Size: 792 651 3197 2579 Fragments arranged by size: 3197 2579 792 651 Bpu10I CC'TnA_GC Cuts at: 2705 2705 Size: 7219 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5344 6209 6507 5344 Size: 865 298 6056 Fragments arranged by size: 6056 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3012 5882 882 Size: 617 1513 2870 2219 Fragments arranged by size: 2870 2219 1513 617 BsaAI yAC'GTr Cuts at: 493 1204 5010 493 Size: 711 3806 2702 Fragments arranged by size: 3806 2702 711 BsaBI GATnn'nnATC Cuts at: 4389 7213 4389 Size: 2824 4395 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5026 5408 6043 275 Size: 53 83 186 936 3493 382 635 1451 Fragments arranged by size: 3493 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2610 5652 6050 6324 6471 6882 2610 Size: 3042 398 274 147 411 2947 Fragments arranged by size: 3042 2947 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3139 3169 1178 Size: 30 165 30 1736 30 5228 Fragments arranged by size: 5228 1736 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2482 2485 2679 2823 1186 Size: 1296 3 194 144 5582 Fragments arranged by size: 5582 1296 194 144 3 BseYI C'CCAG_C Cuts at: 6422 6918 6422 Size: 496 6723 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3308 1163 Size: 573 1572 5074 Fragments arranged by size: 5074 1572 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4669 5430 6458 1540 Size: 275 186 2668 761 1028 2301 Fragments arranged by size: 2668 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5280 5365 6436 5280 Size: 85 1071 6063 Fragments arranged by size: 6063 1071 85 BsmI GAATG_Cn' Cuts at: 3828 4441 4730 4823 3828 Size: 613 289 93 6224 Fragments arranged by size: 6224 613 289 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4538 5280 5365 6436 1391 Size: 399 2748 742 85 1071 2174 Fragments arranged by size: 2748 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2610 6882 2610 Size: 4272 2947 BspHI T'CATG_A Cuts at: 5108 6838 5108 Size: 1730 5489 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4574 844 Size: 3730 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2140 5106 1394 Size: 429 317 2966 3507 Fragments arranged by size: 3507 2966 429 317 BsrDI GCAATG_nn' Cuts at: 65 1248 2058 2778 3037 3188 5714 5888 65 Size: 1183 810 720 259 151 2526 174 1396 Fragments arranged by size: 2526 1396 1183 810 720 259 174 151 BsrFI r'CCGG_y Cuts at: 1700 1810 5863 6050 1700 Size: 110 4053 187 2869 Fragments arranged by size: 4053 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7219 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7219 BssSI C'ACGA_G Cuts at: 4026 5279 6291 4026 Size: 1253 1012 4954 Fragments arranged by size: 4954 1253 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3322 794 Size: 2528 4691 BstBI TT'CG_AA Cuts at: 2540 6023 2540 Size: 3483 3736 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7219 BstYI r'GATC_y Cuts at: 1972 2518 3435 3482 3994 4105 4303 4390 4549 Size: 546 917 47 512 111 198 87 159 Cuts at: 4549 4916 5301 5318 6770 6856 6868 7214 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 917 546 512 385 367 346 198 159 111 87 86 47 17 12 Bsu36I CC'TnA_GG Cuts at: 4308 4308 Size: 7219 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 5710 Fragments arranged by size: 5710 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2689 487 Size: 683 56 1463 5017 Fragments arranged by size: 5017 1463 683 56 BtsI GCAGTG_nn' Cuts at: 2070 4735 5529 5549 2070 Size: 2665 794 20 3740 Fragments arranged by size: 3740 2665 794 20 ClaI AT'CG_AT Cuts at: 1554 3774 4188 1554 Size: 2220 414 4585 Fragments arranged by size: 4585 2220 414 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7184 DraI TTT'AAA Cuts at: 2325 4523 2325 Size: 2198 5021 DraIII CAC_nnn'GTG Cuts at: 3856 3856 Size: 7219 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6226 809 Size: 507 4910 1802 Fragments arranged by size: 4910 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 4666 5555 Size: 54 1425 50 275 18 258 2578 889 Cuts at: 5555 7184 8 Size: 1629 43 Fragments arranged by size: 2578 1629 1425 889 275 258 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4666 1537 Size: 275 2854 4090 Fragments arranged by size: 4090 2854 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2094 2358 2389 5149 1353 Size: 741 264 31 2760 3423 Fragments arranged by size: 3423 2760 741 264 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5806 6180 6326 387 Size: 1064 138 4217 374 146 1280 Fragments arranged by size: 4217 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2031 2507 2682 3297 3380 3687 3698 Size: 393 399 476 175 615 83 307 11 Cuts at: 3698 3913 4094 4425 4512 5282 5879 6666 6934 Size: 215 181 331 87 770 597 787 268 Cuts at: 6934 1239 Size: 1524 Fragments arranged by size: 1524 787 770 615 597 476 399 393 331 307 268 215 181 175 87 83 11 EcoO109I rG'GnC_Cy Cuts at: 2819 2819 Size: 7219 EcoRI G'AATT_C Cuts at: 2870 4528 4906 2870 Size: 1658 378 5183 Fragments arranged by size: 5183 1658 378 EcoRV GAT'ATC Cuts at: 2893 2893 Size: 7219 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2129 4315 2129 Size: 2186 5033 FspI TGC'GCA Cuts at: 5725 5725 Size: 7219 HaeII r_GCGC'y Cuts at: 1678 6366 1678 Size: 4688 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2165 2197 2414 2446 2474 Size: 32 135 32 593 32 217 32 28 Cuts at: 2474 2506 2549 2581 3139 3171 3387 3419 3724 Size: 32 43 32 558 32 216 32 305 Cuts at: 3724 3756 3802 3834 4359 4391 5860 5892 5934 Size: 32 46 32 525 32 1469 32 42 Cuts at: 5934 5966 1373 Size: 32 2626 Fragments arranged by size: 2626 1469 593 558 525 305 217 216 135 46 43 42 32 32 32 32 32 32 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 2184 3524 4563 4809 669 Size: 988 281 246 1340 1039 246 3079 Fragments arranged by size: 3079 1340 1039 988 281 246 246 HindIII A'AGCT_T Cuts at: 748 3232 748 Size: 2484 4735 HpaI GTT'AAC Cuts at: 4809 4809 Size: 7219 KpnI G_GTAC'C Cuts at: 2200 2683 4544 2200 Size: 483 1861 4875 Fragments arranged by size: 4875 1861 483 MfeI C'AATT_G Cuts at: 2349 3465 3501 4818 2349 Size: 1116 36 1317 4750 Fragments arranged by size: 4750 1317 1116 36 MluI A'CGCG_T Cuts at: 7095 7095 Size: 7219 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3867 4083 6333 6517 1305 Size: 2562 216 2250 184 2007 Fragments arranged by size: 2562 2250 2007 216 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5397 Fragments arranged by size: 5397 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2075 3896 5177 5536 5695 518 Size: 648 909 1821 1281 359 159 2042 Fragments arranged by size: 2042 1821 1281 909 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 2024 2031 4214 4635 5312 6460 6705 Size: 406 12 7 2183 421 677 1148 245 Cuts at: 6705 1606 Size: 2120 Fragments arranged by size: 2183 2120 1148 677 421 406 245 12 7 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6667 NdeI CA'TA_TG Cuts at: 387 3318 387 Size: 2931 4288 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7219 NotI GC'GGCC_GC Cuts at: 4666 4666 Size: 7219 NspI r_CATG'y Cuts at: 1132 1229 2167 3478 3886 3979 4577 6122 1132 Size: 97 938 1311 408 93 598 1545 2229 Fragments arranged by size: 2229 1545 1311 938 598 408 97 93 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7219 PciI A'CATG_T Cuts at: 3474 6118 3474 Size: 2644 4575 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7219 PmeI GTTT'AAAC Cuts at: 4523 4523 Size: 7219 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5288 5320 6801 6833 5288 Size: 32 1481 32 5674 Fragments arranged by size: 5674 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2819 2819 Size: 7219 PshAI GACnn'nnGTC Cuts at: 1613 3361 1613 Size: 1748 5471 PsiI TTA'TAA Cuts at: 4789 4789 Size: 7219 PstI C_TGCA'G Cuts at: 830 3687 4060 4571 830 Size: 2857 373 511 3478 Fragments arranged by size: 3478 2857 511 373 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7219 PvuII CAG'CTG Cuts at: 4214 4214 Size: 7219 SacII CC_GC'GG Cuts at: 2025 2025 Size: 7219 SalI G'TCGA_C Cuts at: 1936 2182 3522 4561 1936 Size: 246 1340 1039 4594 Fragments arranged by size: 4594 1340 1039 246 SbfI CC_TGCA'GG Cuts at: 4571 4571 Size: 7219 ScaI AGT'ACT Cuts at: 5467 7179 5467 Size: 1712 5507 SfcI C'TryA_G Cuts at: 826 1045 3657 3683 3744 4056 4567 5702 6383 Size: 219 2612 26 61 312 511 1135 681 Cuts at: 6383 6574 826 Size: 191 1471 Fragments arranged by size: 2612 1471 1135 681 511 312 219 191 61 26 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7219 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7219 SmaI CCC'GGG Cuts at: 1872 4546 1872 Size: 2674 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5323 6224 6486 820 Size: 197 926 3380 901 262 1553 Fragments arranged by size: 3380 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5010 493 Size: 4517 2702 SpeI A'CTAG_T Cuts at: 152 3257 152 Size: 3105 4114 SphI G_CATG'C Cuts at: 1132 4577 1132 Size: 3445 3774 SspI AAT'ATT Cuts at: 5 52 2416 2950 5143 5 Size: 47 2364 534 2193 2081 Fragments arranged by size: 2364 2193 2081 534 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7219 StyI C'CwwG_G Cuts at: 513 1065 4658 513 Size: 552 3593 3074 Fragments arranged by size: 3593 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3036 3639 5246 5263 5416 5601 6046 Size: 1255 1456 603 1607 17 153 185 445 Cuts at: 6046 325 Size: 1498 Fragments arranged by size: 1607 1498 1456 1255 603 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4022 5465 7177 Size: 274 80 33 51 1443 2045 1443 1712 Cuts at: 7177 96 Size: 138 Fragments arranged by size: 2045 1712 1443 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2877 2909 7179 7211 2877 Size: 32 4270 32 2885 Fragments arranged by size: 4270 2885 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2606 1316 Size: 1290 5929 XbaI T'CTAG_A Cuts at: 4334 4393 4555 4334 Size: 59 162 6998 Fragments arranged by size: 6998 162 59 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7219 XmaI C'CCGG_G Cuts at: 1870 4544 1870 Size: 2674 4545 XmnI GAAnn'nnTTC Cuts at: 2520 5348 2520 Size: 2828 4391 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5027 276 Size: 53 83 186 936 3493 2468 Fragments arranged by size: 3493 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI