Restriction map for
pFN21AB7327
(Linear) (Six-base) MAPSORT of: KIBB7327.seq Check: 6004 from: 1 to: 2514
KIBB7327.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 0 178 198 841 1518 2514
Size: 178 20 643 677 996
Fragments arranged by size:
996 677 643 178 20
Acc65I G'GTAC_C
Cuts at: 0 191 674 2514
Size: 191 483 1840
Fragments arranged by size:
1840 483 191
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 1292 1375 1682 1693 1908 2089 2420
Size: 502 790 83 307 11 215 181 331
Cuts at: 2420 2507 2514
Size: 87 7
Fragments arranged by size:
790 502 331 307 215 181 87 83
11 7
AflIII A'CryG_T
Cuts at: 0 1469 2514
Size: 1469 1045
AleI CACnn'nnGTG
Cuts at: 0 70 2514
Size: 70 2444
ApoI r'AATT_y
Cuts at: 0 185 238 339 437 586 865 1759 1788
Size: 185 53 101 98 149 279 894 29
Cuts at: 1788 2289 2481 2514
Size: 501 192 33
Fragments arranged by size:
894 501 279 192 185 149 101 98
53 33 29
AseI AT'TA_AT
Cuts at: 0 725 2042 2311 2514
Size: 725 1317 269 203
Fragments arranged by size:
1317 725 269 203
BamHI G'GATC_C
Cuts at: 0 1477 1989 2514
Size: 1477 512 525
Fragments arranged by size:
1477 525 512
BanI G'GyrC_C
Cuts at: 0 191 674 2514
Size: 191 483 1840
Fragments arranged by size:
1840 483 191
BbsI GAAGACnn'nnnn_
Cuts at: 0 1356 2514
Size: 1356 1158
BclI T'GATC_A
Cuts at: 0 1592 2234 2421 2514
Size: 1592 642 187 93
Fragments arranged by size:
1592 642 187 93
BglII A'GATC_T
Cuts at: 0 513 1430 2385 2514
Size: 513 917 955 129
Fragments arranged by size:
955 917 513 129
BmgBI CAC'GTC
Cuts at: 0 992 2514
Size: 992 1522
BmrI ACTGGGnnnn_n'
Cuts at: 0 595 2088 2514
Size: 595 1493 426
Fragments arranged by size:
1493 595 426
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 677 2514
Size: 26 651 1837
Fragments arranged by size:
1837 651 26
Bpu10I CC'TnA_GC
Cuts at: 0 700 2514
Size: 700 1814
BsaI GGTCTCn'nnnn_
Cuts at: 0 1007 2514
Size: 1007 1507
BsaBI GATnn'nnATC
Cuts at: 0 2384 2514
Size: 2384 130
BsaWI w'CCGG_w
Cuts at: 0 605 2514
Size: 605 1909
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1134 1164 2514
Size: 1134 30 1350
Fragments arranged by size:
1350 1134 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 477 480 674 818 2514
Size: 477 3 194 144 1696
Fragments arranged by size:
1696 477 194 144 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1303 2514
Size: 1303 1211
BsmI GAATG_Cn'
Cuts at: 0 1823 2436 2514
Size: 1823 613 78
Fragments arranged by size:
1823 613 78
BspEI T'CCGG_A
Cuts at: 0 605 2514
Size: 605 1909
BsrBI CCG'CTC
Cuts at: 0 135 2514
Size: 135 2379
BsrDI GCAATG_nn'
Cuts at: 0 53 773 1032 1183 2514
Size: 53 720 259 151 1331
Fragments arranged by size:
1331 720 259 151 53
BssSI C'ACGA_G
Cuts at: 0 2021 2514
Size: 2021 493
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1317 2514
Size: 1317 1197
BstBI TT'CG_AA
Cuts at: 0 535 2514
Size: 535 1979
BstYI r'GATC_y
Cuts at: 0 513 1430 1477 1989 2100 2298 2385 2514
Size: 513 917 47 512 111 198 87 129
Fragments arranged by size:
917 513 512 198 129 111 87 47
Bsu36I CC'TnA_GG
Cuts at: 0 2303 2514
Size: 2303 211
BtgI C'CryG_G
Cuts at: 0 17 2514
Size: 17 2497
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 684 2514
Size: 684 1830
BtsI GCAGTG_nn'
Cuts at: 0 65 2514
Size: 65 2449
ClaI AT'CG_AT
Cuts at: 0 1769 2183 2514
Size: 1769 414 331
Fragments arranged by size:
1769 414 331
DraI TTT'AAA
Cuts at: 0 320 2514
Size: 320 2194
DraIII CAC_nnn'GTG
Cuts at: 0 1851 2514
Size: 1851 663
EaeI y'GGCC_r
Cuts at: 0 83 2514
Size: 83 2431
EarI CTCTTCn'nnn_
Cuts at: 0 89 353 384 2514
Size: 89 264 31 2130
Fragments arranged by size:
2130 264 89 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 502 677 1292 1375 1682 1693 1908
Size: 26 476 175 615 83 307 11 215
Cuts at: 1908 2089 2420 2507 2514
Size: 181 331 87 7
Fragments arranged by size:
615 476 331 307 215 181 175 87
83 26 11 7
EcoO109I rG'GnC_Cy
Cuts at: 0 814 2514
Size: 814 1700
EcoRI G'AATT_C
Cuts at: 0 865 2514
Size: 865 1649
EcoRV GAT'ATC
Cuts at: 0 888 2514
Size: 888 1626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 124 2310 2514
Size: 124 2186 204
Fragments arranged by size:
2186 204 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 409 441 469 501 544 576
Size: 160 32 217 32 28 32 43 32
Cuts at: 576 1134 1166 1382 1414 1719 1751 1797 1829
Size: 558 32 216 32 305 32 46 32
Cuts at: 1829 2354 2386 2514
Size: 525 32 128
Fragments arranged by size:
558 525 305 217 216 160 128 46
43 32 32 32 32 32 32 32
32 32 28
HincII GTy'rAC
Cuts at: 0 179 1519 2514
Size: 179 1340 995
Fragments arranged by size:
1340 995 179
HindIII A'AGCT_T
Cuts at: 0 1227 2514
Size: 1227 1287
KpnI G_GTAC'C
Cuts at: 0 195 678 2514
Size: 195 483 1836
Fragments arranged by size:
1836 483 195
MfeI C'AATT_G
Cuts at: 0 344 1460 1496 2514
Size: 344 1116 36 1018
Fragments arranged by size:
1116 1018 344 36
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1862 2078 2514
Size: 1862 216 436
Fragments arranged by size:
1862 436 216
MslI CAynn'nnrTG
Cuts at: 0 70 1891 2514
Size: 70 1821 623
Fragments arranged by size:
1821 623 70
MspA1I CmG'CkG
Cuts at: 0 7 19 26 2209 2514
Size: 7 12 7 2183 305
Fragments arranged by size:
2183 305 12 7 7
NdeI CA'TA_TG
Cuts at: 0 1313 2514
Size: 1313 1201
NspI r_CATG'y
Cuts at: 0 162 1473 1881 1974 2514
Size: 162 1311 408 93 540
Fragments arranged by size:
1311 540 408 162 93
PciI A'CATG_T
Cuts at: 0 1469 2514
Size: 1469 1045
PpuMI rG'GwC_Cy
Cuts at: 0 814 2514
Size: 814 1700
PshAI GACnn'nnGTC
Cuts at: 0 1356 2514
Size: 1356 1158
PstI C_TGCA'G
Cuts at: 0 1682 2055 2514
Size: 1682 373 459
Fragments arranged by size:
1682 459 373
PvuII CAG'CTG
Cuts at: 0 2209 2514
Size: 2209 305
SacII CC_GC'GG
Cuts at: 0 20 2514
Size: 20 2494
SalI G'TCGA_C
Cuts at: 0 177 1517 2514
Size: 177 1340 997
Fragments arranged by size:
1340 997 177
SfcI C'TryA_G
Cuts at: 0 1652 1678 1739 2051 2514
Size: 1652 26 61 312 463
Fragments arranged by size:
1652 463 312 61 26
SpeI A'CTAG_T
Cuts at: 0 1252 2514
Size: 1252 1262
SspI AAT'ATT
Cuts at: 0 411 945 2514
Size: 411 534 1569
Fragments arranged by size:
1569 534 411
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1031 1634 2514
Size: 1031 603 880
Fragments arranged by size:
1031 880 603
TatI w'GTAC_w
Cuts at: 0 2017 2514
Size: 2017 497
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 872 904 2514
Size: 872 32 1610
Fragments arranged by size:
1610 872 32
Tth111I GACn'n_nGTC
Cuts at: 0 601 2514
Size: 601 1913
XbaI T'CTAG_A
Cuts at: 0 2329 2388 2514
Size: 2329 59 126
Fragments arranged by size:
2329 126 59
XmnI GAAnn'nnTTC
Cuts at: 0 515 2514
Size: 515 1999
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI ApoI AseI BamHI
BanI BbsI BclI BglII BmgBI BmrI BpmI Bpu10I
BsaI BsaBI BsaWI BsaXI BseRI BsgI BsmI BspEI
BsrBI BsrDI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII EaeI EarI Eco57MI
EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII KpnI
MfeI MmeI MslI MspA1I NdeI NspI PciI PpuMI
PshAI PstI PvuII SacII SalI SfcI SpeI SspI
TaqII TatI TstI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BanII BbeI BbvCI BcgI BciVI BfrBI BglI
BlpI Bme1580I BmtI BplI BpuEI BsaAI BsaHI BseYI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrFI
BsrGI BssHII BstEII BstXI BstZ17I CspCI DrdI EagI
EciI EcoICRI EcoNI FseI FspI FspAI HaeII HpaI
KasI MluI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SphI SrfI StuI
StyI SwaI XcmI XhoI XmaI ZraI