Restriction map for pFN21AE0110
 (Circular) (Six-base) MAPSORT of: pFN21AE0110.seq  Check: 2787  from: 1  to: 5482



pFN21AE0110  5482 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:44  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3292     278 
   Size:         53      83     186     936    1756    2468

  Fragments arranged by size: 

                2468    1756     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2825    1937 
   Size:        888    4594
 
Acc65I G'GTAC_C

Cuts at:   2803    2803 
   Size:       5482
 
AclI AA'CG_TT

Cuts at:   1563    3609    3982    1563 
   Size:       2046     373    3063

  Fragments arranged by size: 

                3063    2046     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2229    3545    4929    5197    1632 
   Size:        597    1316    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1316     597     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5285
 
AflIII A'CryG_T

Cuts at:   1203    4381    5358    1203 
   Size:       3178     977    1327

  Fragments arranged by size: 

                3178    1327     977
 
AgeI A'CCGG_T

Cuts at:   4313    4313 
   Size:       5482
 
AhdI GACnn_n'nnGTC

Cuts at:   4211    4211 
   Size:       5482
 
AleI CACnn'nnGTG

Cuts at:   1166    2531    1166 
   Size:       1365    4117
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5482
 
AlwNI CAG_nnn'CTG

Cuts at:   3225    4797    3225 
   Size:       1572    3910
 
ApaLI G'TGCA_C

Cuts at:   3539    4695    3539 
   Size:       1156    4326
 
ApoI r'AATT_y

Cuts at:   1493    2195    2379    2791    3017    3169    5278    1493 
   Size:        702     184     412     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     702     412     226     184     152
 
AseI AT'TA_AT

Cuts at:    160    1032    2712    4036     160 
   Size:        872    1680    1324    1606

  Fragments arranged by size: 

                1680    1606    1324     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5482
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2807    1870 
   Size:         73     864    4545

  Fragments arranged by size: 

                4545     864      73
 
BamHI G'GATC_C

Cuts at:   2812    3179    2812 
   Size:        367    5115
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2356    2803    4258     618 
   Size:        325     217     627     569     447    1455    1842

  Fragments arranged by size: 

                1842    1455     627     569     447     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2644    2801    1391 
   Size:       1253     157    4072

  Fragments arranged by size: 

                4072    1253     157
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2555     928 
   Size:        971     656    3855

  Fragments arranged by size: 

                3855     971     656
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3673    3707    1903 
   Size:         34    1736      34    3678

  Fragments arranged by size: 

                3678    1736      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3374    4584    3374 
   Size:       1210    4272
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5482
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4093     136 
   Size:        107     122      71     631     605    2421    1525

  Fragments arranged by size: 

                2421    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2041    5477    2041 
   Size:       3436    2046
 
BlpI GC'TnA_GC

Cuts at:   2467    2899    2467 
   Size:        432    5050
 
Bme1580I G_kGCm'C

Cuts at:   1790    2299    2579    3543    4699    1790 
   Size:        509     280     964    1156    2573

  Fragments arranged by size: 

                2573    1156     964     509     280
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5482
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2688    3213    4171 
   Size:        416      53      83     399      24    1271     525     958

Cuts at:   4171     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1271     958     525     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5482
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2763    2763 
   Size:       5482
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4142    1239 
   Size:       2903    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2628    3607    4472    4770    2628 
   Size:        979     865     298    3340

  Fragments arranged by size: 

                3340     979     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4145     882 
   Size:        617    2646    2219

  Fragments arranged by size: 

                2646    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2111    3273     493 
   Size:        711     907    1162    2702

  Fragments arranged by size: 

                2702    1162     907     711
 
BsaBI GATnn'nnATC

Cuts at:   2429    5476    2429 
   Size:       3047    2435
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3289    3671    4306     275 
   Size:         53      83     186     936    1756     382     635    1451

  Fragments arranged by size: 

                1756    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2397    3915    4313    4587    4734    5145    2397 
   Size:       1518     398     274     147     411    2734

  Fragments arranged by size: 

                2734    1518     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5257

  Fragments arranged by size: 

                5257     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2609    1186 
   Size:       1423    4059
 
BseYI C'CCAG_C

Cuts at:   2659    4685    5181    2659 
   Size:       2026     496    2960

  Fragments arranged by size: 

                2960    2026     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4909
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2932    3693    4721    1540 
   Size:        275     186     931     761    1028    2301

  Fragments arranged by size: 

                2301    1028     931     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2419    3543    3628    4699    2419 
   Size:       1124      85    1071    3202

  Fragments arranged by size: 

                3202    1124    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2393    2993    3086    2393 
   Size:        600      93    4789

  Fragments arranged by size: 

                4789     600      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2299    2419    2579    2644    2801    3543    3628 
   Size:        399     509     120     160      65     157     742      85

Cuts at:   3628    4699    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     509     399     160     157     120
                  85      65
 
BspEI T'CCGG_A

Cuts at:   5145    5145 
   Size:       5482
 
BspHI T'CATG_A

Cuts at:   2604    3371    5101    2604 
   Size:        767    1730    2985

  Fragments arranged by size: 

                2985    1730     767
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2837     844 
   Size:       1993    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2257    3369    1394 
   Size:        429     434    1112    3507

  Fragments arranged by size: 

                3507    1112     434     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2567    3977    4151      65 
   Size:       1183    1319    1410     174    1396

  Fragments arranged by size: 

                1410    1396    1319    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4126    4313    1700 
   Size:        110    2316     187    2869

  Fragments arranged by size: 

                2869    2316     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5482
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5482
 
BssSI C'ACGA_G

Cuts at:   3542    4554    3542 
   Size:       1012    4470
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2018     794 
   Size:       1224    4258
 
BstBI TT'CG_AA

Cuts at:   4286    4286 
   Size:       5482
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5482
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2533    2533 
   Size:       5482
 
BstYI r'GATC_y

Cuts at:   1972    2041    2812    3179    3564    3581    5033    5119    5131 
   Size:         69     771     367     385      17    1452      86      12

Cuts at:   5131    5477    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     771     385     367     346      86      69
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2592    2592 
   Size:       5482
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3991

  Fragments arranged by size: 

                3991     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4743

  Fragments arranged by size: 

                4743     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2998    3792    3812    2998 
   Size:        794      20    4668

  Fragments arranged by size: 

                4668     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5482
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5447
 
DraI TTT'AAA

Cuts at:   2786    2786 
   Size:       5482
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4489     809 
   Size:        507    3173    1802

  Fragments arranged by size: 

                3173    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2929    3818    5447 
   Size:         54    1425      50     275      18    1099     889    1629

Cuts at:   5447       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1099     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2929    1537 
   Size:        275    1117    4090

  Fragments arranged by size: 

                4090    1117     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3412    1353 
   Size:       2059    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4069    4443    4589     387 
   Size:       1064     138    2480     374     146    1280

  Fragments arranged by size: 

                2480    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2229    3545    4142    4929    5197    1239 
   Size:        393     597    1316     597     787     268    1524

  Fragments arranged by size: 

                1524    1316     787     597     597     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2477    2477 
   Size:       5482
 
EcoRI G'AATT_C

Cuts at:   2791    3169    2791 
   Size:        378    5104
 
EcoRV GAT'ATC

Cuts at:   2203    2203 
   Size:       5482
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2572    2572 
   Size:       5482
 
FspI TGC'GCA

Cuts at:   3988    3988 
   Size:       5482
 
HaeII r_GCGC'y

Cuts at:   1678    4629    1678 
   Size:       2951    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4123    4155    4197    4229    1373 
   Size:         32     135      32    2551      32      42      32    2626

  Fragments arranged by size: 

                2626    2551     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2826    3072     669 
   Size:        988     281     888     246    3079

  Fragments arranged by size: 

                3079     988     888     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5482
 
HpaI GTT'AAC

Cuts at:   3072    3072 
   Size:       5482
 
KpnI G_GTAC'C

Cuts at:   2807    2807 
   Size:       5482
 
MfeI C'AATT_G

Cuts at:   3081    3081 
   Size:       5482
 
MluI A'CGCG_T

Cuts at:   5358    5358 
   Size:       5482
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4596    4780    1305 
   Size:       3291     184    2007

  Fragments arranged by size: 

                3291    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3660

  Fragments arranged by size: 

                3660    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2531    3440    3799    3958     518 
   Size:        648    1365     909     359     159    2042

  Fragments arranged by size: 

                2042    1365     909     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2075    2898    3575    4723    4968    1606 
   Size:        469     823     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     823     677     469     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3991

  Fragments arranged by size: 

                3991     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5482
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5482
 
NotI GC'GGCC_GC

Cuts at:   2929    2929 
   Size:       5482
 
NspI r_CATG'y

Cuts at:   1132    1229    2016    2659    2840    4385    1132 
   Size:         97     787     643     181    1545    2229

  Fragments arranged by size: 

                2229    1545     787     643     181      97
 
PasI CC'CwG_GG

Cuts at:   1348    2473    1348 
   Size:       1125    4357
 
PciI A'CATG_T

Cuts at:   4381    4381 
   Size:       5482
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5482
 
PfoI T'CCnGG_A

Cuts at:   2406    2406 
   Size:       5482
 
PmeI GTTT'AAAC

Cuts at:   2786    2786 
   Size:       5482
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2537    2569    3551    3583    5064    5096    2537 
   Size:         32     982      32    1481      32    2923

  Fragments arranged by size: 

                2923    1481     982      32      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5482
 
PsiI TTA'TAA

Cuts at:   3052    3052 
   Size:       5482
 
PstI C_TGCA'G

Cuts at:    830    2239    2772    2834     830 
   Size:       1409     533      62    3478

  Fragments arranged by size: 

                3478    1409     533      62
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5482
 
PvuII CAG'CTG

Cuts at:   2075    2075 
   Size:       5482
 
SalI G'TCGA_C

Cuts at:   1936    2824    1936 
   Size:        888    4594
 
SbfI CC_TGCA'GG

Cuts at:   2834    2834 
   Size:       5482
 
ScaI AGT'ACT

Cuts at:   3730    5442    3730 
   Size:       1712    3770
 
SfcI C'TryA_G

Cuts at:    826    1045    2235    2768    2830    3965    4646    4837     826 
   Size:        219    1190     533      62    1135     681     191    1471

  Fragments arranged by size: 

                1471    1190    1135     681     533     219     191      62
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5482
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5482
 
SmaI CCC'GGG

Cuts at:   1872    2809    1872 
   Size:        937    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2643    3586    4487    4749     820 
   Size:        197     926     700     943     901     262    1553

  Fragments arranged by size: 

                1553     943     926     901     700     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3273     493 
   Size:       2780    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5482
 
SphI G_CATG'C

Cuts at:   1132    2016    2659    2840    1132 
   Size:        884     643     181    3774

  Fragments arranged by size: 

                3774     884     643     181
 
SspI AAT'ATT

Cuts at:      5      52    2220    3406       5 
   Size:         47    2168    1186    2081

  Fragments arranged by size: 

                2168    2081    1186      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5482
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2921     513 
   Size:        552     939     917    3074

  Fragments arranged by size: 

                3074     939     917     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3509    3526    3679    3864    4309     325 
   Size:       1255    1929      17     153     185     445    1498

  Fragments arranged by size: 

                1929    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3728    5440      96 
   Size:        274      80      33      51    1443    1751    1712     138

  Fragments arranged by size: 

                1751    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2475    2507    5442    5474    2475 
   Size:         32    2935      32    2483

  Fragments arranged by size: 

                2935    2483      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5482
 
XbaI T'CTAG_A

Cuts at:   2818    2818 
   Size:       5482
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5482
 
XmaI C'CCGG_G

Cuts at:   1870    2807    1870 
   Size:        937    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2367    2552    3611    2367 
   Size:        185    1059    4238

  Fragments arranged by size: 

                4238    1059     185
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3290     276 
   Size:         53      83     186     936    1756    2468

  Fragments arranged by size: 

                2468    1756     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI
     PstI     PvuI    PvuII     SalI     SbfI     ScaI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI   Bpu10I    BsiWI    BsmBI  BstZ17I   DraIII  EcoICRI
    EcoNI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI     PmlI    PpuMI   PspOMI    PspXI     PsrI    RsrII
     SacI    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI
     SwaI     XcmI
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