Restriction map for
pFN21AB8892
(Circular) (Six-base) MAPSORT of: pFN21AB8892.seq Check: 5887 from: 1 to: 5350
pFN21AB8892 5350 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3160 278
Size: 53 83 186 936 1624 2468
Fragments arranged by size:
2468 1624 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2693 1937
Size: 756 4594
Acc65I G'GTAC_C
Cuts at: 2671 2671
Size: 5350
AclI AA'CG_TT
Cuts at: 1563 3477 3850 1563
Size: 1914 373 3063
Fragments arranged by size:
3063 1914 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3413 4797 5065 1632
Size: 1781 1384 268 1917
Fragments arranged by size:
1917 1781 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5153
AflIII A'CryG_T
Cuts at: 1203 4249 5226 1203
Size: 3046 977 1327
Fragments arranged by size:
3046 1327 977
AgeI A'CCGG_T
Cuts at: 4181 4181
Size: 5350
AhdI GACnn_n'nnGTC
Cuts at: 4079 4079
Size: 5350
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5350
AlwNI CAG_nnn'CTG
Cuts at: 3093 4665 3093
Size: 1572 3778
ApaI G_GGCC'C
Cuts at: 2582 2582
Size: 5350
ApaLI G'TGCA_C
Cuts at: 3407 4563 3407
Size: 1156 4194
ApoI r'AATT_y
Cuts at: 1493 2659 2885 3037 5146 1493
Size: 1166 226 152 2109 1697
Fragments arranged by size:
2109 1697 1166 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3904 160
Size: 872 2872 1606
Fragments arranged by size:
2872 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5350
AvaI C'yCGr_G
Cuts at: 1870 1943 2249 2480 2675 1870
Size: 73 306 231 195 4545
Fragments arranged by size:
4545 306 231 195 73
BamHI G'GATC_C
Cuts at: 2680 3047 2680
Size: 367 4983
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2671 4126 618
Size: 325 217 627 884 1455 1842
Fragments arranged by size:
1842 1455 884 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2150 2249 2582 2669 1391
Size: 759 99 333 87 4072
Fragments arranged by size:
4072 759 333 99 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4379
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3541 3575 1903
Size: 34 1604 34 3678
Fragments arranged by size:
3678 1604 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3242 4452 3242
Size: 1210 4140
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5350
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2280 2492 3961
Size: 107 122 71 631 605 608 212 1469
Cuts at: 3961 136
Size: 1525
Fragments arranged by size:
1525 1469 631 608 605 212 122 107
71
BglII A'GATC_T
Cuts at: 5345 5345
Size: 5350
BlpI GC'TnA_GC
Cuts at: 2144 2767 2144
Size: 623 4727
Bme1580I G_kGCm'C
Cuts at: 1790 2582 3411 4567 1790
Size: 792 829 1156 2573
Fragments arranged by size:
2573 1156 829 792
BmgBI CAC'GTC
Cuts at: 1318 2449 1318
Size: 1131 4219
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2576 3081 4039
Size: 416 53 83 399 24 1159 505 958
Cuts at: 4039 442
Size: 1753
Fragments arranged by size:
1753 1159 958 505 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2279 2657 4010 1239
Size: 1040 378 1353 2579
Fragments arranged by size:
2579 1353 1040 378
Bpu10I CC'TnA_GC
Cuts at: 2296 2296
Size: 5350
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2486 3475 4340 4638 2486
Size: 989 865 298 3198
Fragments arranged by size:
3198 989 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4013 882
Size: 617 2514 2219
Fragments arranged by size:
2514 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3141 493
Size: 711 1937 2702
Fragments arranged by size:
2702 1937 711
BsaBI GATnn'nnATC
Cuts at: 5344 5344
Size: 5350
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2229 3157 3539 4174
Size: 53 83 186 936 696 928 382 635
Cuts at: 4174 275
Size: 1451
Fragments arranged by size:
1451 936 928 696 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3783 4181 4455 4602 5013 3783
Size: 398 274 147 411 4120
Fragments arranged by size:
4120 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5125
Fragments arranged by size:
5125 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5350
BseYI C'CCAG_C
Cuts at: 4553 5049 4553
Size: 496 4854
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4777
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2199 2275 2382 2521 2800 3561
Size: 275 186 198 76 107 139 279 761
Cuts at: 3561 4589 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 279 275 198 186 139
107 76
BsiHKAI G_wGCw'C
Cuts at: 3411 3496 4567 3411
Size: 85 1071 4194
Fragments arranged by size:
4194 1071 85
BsmI GAATG_Cn'
Cuts at: 2342 2861 2954 2342
Size: 519 93 4738
Fragments arranged by size:
4738 519 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2311 2311
Size: 5350
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2150 2249 2582 2669 3411 3496 4567
Size: 399 360 99 333 87 742 85 1071
Cuts at: 4567 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 360 333 99 87
85
BspEI T'CCGG_A
Cuts at: 5013 5013
Size: 5350
BspHI T'CATG_A
Cuts at: 3239 4969 3239
Size: 1730 3620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2705 844
Size: 1861 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2314 3237 1394
Size: 429 491 923 3507
Fragments arranged by size:
3507 923 491 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3845 4019 65
Size: 1183 2597 174 1396
Fragments arranged by size:
2597 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2283 2377 2609 3994 4181 1700
Size: 110 473 94 232 1385 187 2869
Fragments arranged by size:
2869 1385 473 232 187 110 94
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5350
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5350
BssSI C'ACGA_G
Cuts at: 2012 3410 4422 2012
Size: 1398 1012 2940
Fragments arranged by size:
2940 1398 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2068 794
Size: 1274 4076
BstBI TT'CG_AA
Cuts at: 4154 4154
Size: 5350
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5350
BstYI r'GATC_y
Cuts at: 1972 2680 3047 3432 3449 4901 4987 4999 5345
Size: 708 367 385 17 1452 86 12 346
Cuts at: 5345 1972
Size: 1977
Fragments arranged by size:
1977 1452 708 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2193 513
Size: 552 1128 3670
Fragments arranged by size:
3670 1128 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2416 487
Size: 683 56 1190 3421
Fragments arranged by size:
3421 1190 683 56
BtsI GCAGTG_nn'
Cuts at: 2866 3660 3680 2866
Size: 794 20 4536
Fragments arranged by size:
4536 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5350
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5315
DraI TTT'AAA
Cuts at: 2654 2654
Size: 5350
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4357 809
Size: 507 3041 1802
Fragments arranged by size:
3041 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2196 2272 2281
Size: 54 1425 50 275 18 366 76 9
Cuts at: 2281 2379 2454 2518 2615 2797 3686 5315 8
Size: 98 75 64 97 182 889 1629 43
Fragments arranged by size:
1629 1425 889 366 275 182 98 97
76 75 64 54 50 43 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2196 2272 2379 2518 2797 1537
Size: 275 384 76 107 139 279 4090
Fragments arranged by size:
4090 384 279 275 139 107 76
EarI CTCTTCn'nnn_
Cuts at: 1353 2093 2585 3280 1353
Size: 740 492 695 3423
Fragments arranged by size:
3423 740 695 492
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2302 2525 3937 4311 4457 387
Size: 1064 138 713 223 1412 374 146 1280
Fragments arranged by size:
1412 1280 1064 713 374 223 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2279 2657 3413 4010 4797 5065 1239
Size: 393 647 378 756 597 787 268 1524
Fragments arranged by size:
1524 787 756 647 597 393 378 268
EcoO109I rG'GnC_Cy
Cuts at: 2121 2300 2121
Size: 179 5171
EcoRI G'AATT_C
Cuts at: 2659 3037 2659
Size: 378 4972
FspI TGC'GCA
Cuts at: 2061 3856 2061
Size: 1795 3555
HaeII r_GCGC'y
Cuts at: 1678 2267 2636 4497 1678
Size: 589 369 1861 2531
Fragments arranged by size:
2531 1861 589 369
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2392 2424 2453 2485 3991
Size: 32 135 32 820 32 29 32 1506
Cuts at: 3991 4023 4065 4097 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1506 820 135 42 32 32 32
32 32 32 29
HincII GTy'rAC
Cuts at: 669 1657 1938 2694 2940 669
Size: 988 281 756 246 3079
Fragments arranged by size:
3079 988 756 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5350
HpaI GTT'AAC
Cuts at: 2940 2940
Size: 5350
KpnI G_GTAC'C
Cuts at: 2675 2675
Size: 5350
MfeI C'AATT_G
Cuts at: 2949 2949
Size: 5350
MluI A'CGCG_T
Cuts at: 5226 5226
Size: 5350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4464 4648 1305
Size: 3159 184 2007
Fragments arranged by size:
3159 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2456 10
Size: 54 1425 343 624 2904
Fragments arranged by size:
2904 1425 624 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3308 3667 3826 518
Size: 648 2142 359 159 2042
Fragments arranged by size:
2142 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2195 2201 2766 3443 4591 4836 1606
Size: 589 6 565 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 589 565 245 6
NaeI GCC'GGC
Cuts at: 2285 2379 2285
Size: 94 5256
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4798
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5350
NgoMIV G'CCGG_C
Cuts at: 2283 2377 2283
Size: 94 5256
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5350
NotI GC'GGCC_GC
Cuts at: 2196 2797 2196
Size: 601 4749
NspI r_CATG'y
Cuts at: 1132 1229 2708 4253 1132
Size: 97 1479 1545 2229
Fragments arranged by size:
2229 1545 1479 97
PasI CC'CwG_GG
Cuts at: 1348 2575 1348
Size: 1227 4123
PciI A'CATG_T
Cuts at: 4249 4249
Size: 5350
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5350
PmeI GTTT'AAAC
Cuts at: 2654 2654
Size: 5350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2070 2102 3419 3451 4932 4964 2070
Size: 32 1317 32 1481 32 2456
Fragments arranged by size:
2456 1481 1317 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2300 2300
Size: 5350
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5350
PsiI TTA'TAA
Cuts at: 2920 2920
Size: 5350
PspOMI G'GGCC_C
Cuts at: 2578 2578
Size: 5350
PstI C_TGCA'G
Cuts at: 830 2206 2550 2702 830
Size: 1376 344 152 3478
Fragments arranged by size:
3478 1376 344 152
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5350
RsrII CG'GwC_CG
Cuts at: 2093 2093
Size: 5350
SacII CC_GC'GG
Cuts at: 2196 2196
Size: 5350
SalI G'TCGA_C
Cuts at: 1936 2692 1936
Size: 756 4594
SbfI CC_TGCA'GG
Cuts at: 2702 2702
Size: 5350
ScaI AGT'ACT
Cuts at: 3598 5310 3598
Size: 1712 3638
SexAI A'CCwGG_T
Cuts at: 2646 2646
Size: 5350
SfcI C'TryA_G
Cuts at: 826 1045 2202 2546 2698 3833 4514 4705 826
Size: 219 1157 344 152 1135 681 191 1471
Fragments arranged by size:
1471 1157 1135 681 344 219 191 152
SfiI GGCCn_nnn'nGGCC
Cuts at: 2280 2280
Size: 5350
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5350
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5350
SmaI CCC'GGG
Cuts at: 1872 2251 2677 1872
Size: 379 426 4545
Fragments arranged by size:
4545 426 379
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2501 3454 4355 4617 820
Size: 197 926 558 953 901 262 1553
Fragments arranged by size:
1553 953 926 901 558 262 197
SnaBI TAC'GTA
Cuts at: 493 3141 493
Size: 2648 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5350
SphI G_CATG'C
Cuts at: 1132 2708 1132
Size: 1576 3774
SspI AAT'ATT
Cuts at: 5 52 3274 5
Size: 47 3222 2081
Fragments arranged by size:
3222 2081 47
StuI AGG'CCT
Cuts at: 1517 2236 2486 1517
Size: 719 250 4381
Fragments arranged by size:
4381 719 250
StyI C'CwwG_G
Cuts at: 513 1065 2457 2789 513
Size: 552 1392 332 3074
Fragments arranged by size:
3074 1392 552 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2081 3377 3394 3547 3732 4177 325
Size: 1255 501 1296 17 153 185 445 1498
Fragments arranged by size:
1498 1296 1255 501 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3596 5308 96
Size: 274 80 33 51 1443 1619 1712 138
Fragments arranged by size:
1712 1619 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5310 5342 5310
Size: 32 5318
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5350
XbaI T'CTAG_A
Cuts at: 2686 2686
Size: 5350
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5350
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5350
XmaI C'CCGG_G
Cuts at: 1870 2249 2675 1870
Size: 379 426 4545
Fragments arranged by size:
4545 426 379
XmnI GAAnn'nnTTC
Cuts at: 2562 3479 2562
Size: 917 4433
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3158 276
Size: 53 83 186 936 1624 2468
Fragments arranged by size:
2468 1624 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI RsrII SacII SalI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PspXI PsrI PvuII SacI
SanDI SapI SfoI SrfI SwaI