(Circular) (Six-base) MAPSORT of: pFN21AB6499.seq Check: 1415 from: 1 to: 5014 pFN21AB6499 5014 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2824 278 Size: 53 83 186 936 1288 2468 Fragments arranged by size: 2468 1288 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2268 2357 1937 Size: 331 89 4594 Fragments arranged by size: 4594 331 89 Acc65I G'GTAC_C Cuts at: 2335 2335 Size: 5014 AclI AA'CG_TT Cuts at: 1563 3141 3514 1563 Size: 1578 373 3063 Fragments arranged by size: 3063 1578 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2201 2297 3077 4461 4729 1632 Size: 569 96 780 1384 268 1917 Fragments arranged by size: 1917 1384 780 569 268 96 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4817 AflIII A'CryG_T Cuts at: 1203 3913 4890 1203 Size: 2710 977 1327 Fragments arranged by size: 2710 1327 977 AgeI A'CCGG_T Cuts at: 3845 3845 Size: 5014 AhdI GACnn_n'nnGTC Cuts at: 3743 3743 Size: 5014 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5014 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5014 AlwNI CAG_nnn'CTG Cuts at: 2757 4329 2757 Size: 1572 3442 ApaLI G'TGCA_C Cuts at: 3071 4227 3071 Size: 1156 3858 ApoI r'AATT_y Cuts at: 1493 2323 2549 2701 4810 1493 Size: 830 226 152 2109 1697 Fragments arranged by size: 2109 1697 830 226 152 AseI AT'TA_AT Cuts at: 160 1032 3568 160 Size: 872 2536 1606 Fragments arranged by size: 2536 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5014 AvaI C'yCGr_G Cuts at: 1870 1943 2339 1870 Size: 73 396 4545 Fragments arranged by size: 4545 396 73 BamHI G'GATC_C Cuts at: 2344 2711 2344 Size: 367 4647 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2112 2335 3790 618 Size: 325 217 627 325 223 1455 1842 Fragments arranged by size: 1842 1455 627 325 325 223 217 BanII G_rGCy'C Cuts at: 1391 2333 1391 Size: 942 4072 BbeI G_GCGC'C Cuts at: 2116 2116 Size: 5014 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4043 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3205 3239 1903 Size: 34 1268 34 3678 Fragments arranged by size: 3678 1268 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2906 4116 2906 Size: 1210 3804 BclI T'GATC_A Cuts at: 1548 1548 Size: 5014 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3625 136 Size: 107 122 71 631 605 1953 1525 Fragments arranged by size: 1953 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5009 5009 Size: 5014 BlpI GC'TnA_GC Cuts at: 2052 2431 2052 Size: 379 4635 Bme1580I G_kGCm'C Cuts at: 1790 3075 4231 1790 Size: 1285 1156 2573 Fragments arranged by size: 2573 1285 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5014 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2045 2745 3703 Size: 416 53 83 399 24 628 700 958 Cuts at: 3703 442 Size: 1753 Fragments arranged by size: 1753 958 700 628 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5014 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3674 1239 Size: 2435 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3139 4004 4302 3139 Size: 865 298 3851 Fragments arranged by size: 3851 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2156 3677 882 Size: 617 657 1521 2219 Fragments arranged by size: 2219 1521 657 617 BsaAI yAC'GTr Cuts at: 493 1204 2805 493 Size: 711 1601 2702 Fragments arranged by size: 2702 1601 711 BsaBI GATnn'nnATC Cuts at: 5008 5008 Size: 5014 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2113 2821 3203 3838 Size: 53 83 186 936 580 708 382 635 Cuts at: 3838 275 Size: 1451 Fragments arranged by size: 1451 936 708 635 580 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3447 3845 4119 4266 4677 3447 Size: 398 274 147 411 3784 Fragments arranged by size: 3784 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4789 Fragments arranged by size: 4789 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 1186 Size: 991 4023 BseYI C'CCAG_C Cuts at: 4217 4713 4217 Size: 496 4518 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4441 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2464 3225 4253 1540 Size: 275 186 463 761 1028 2301 Fragments arranged by size: 2301 1028 761 463 275 186 BsiHKAI G_wGCw'C Cuts at: 2232 3075 3160 4231 2232 Size: 843 85 1071 3015 Fragments arranged by size: 3015 1071 843 85 BsmI GAATG_Cn' Cuts at: 2525 2618 2525 Size: 93 4921 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2333 3075 3160 4231 1391 Size: 399 442 101 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 442 399 101 85 BspEI T'CCGG_A Cuts at: 4677 4677 Size: 5014 BspHI T'CATG_A Cuts at: 2903 4633 2903 Size: 1730 3284 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2369 844 Size: 1525 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2901 1394 Size: 429 1078 3507 Fragments arranged by size: 3507 1078 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3509 3683 65 Size: 1183 2261 174 1396 Fragments arranged by size: 2261 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3658 3845 1700 Size: 110 1848 187 2869 Fragments arranged by size: 2869 1848 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5014 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5014 BssSI C'ACGA_G Cuts at: 3074 4086 3074 Size: 1012 4002 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5014 BstBI TT'CG_AA Cuts at: 3818 3818 Size: 5014 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5014 BstYI r'GATC_y Cuts at: 1972 2344 2711 3096 3113 4565 4651 4663 5009 Size: 372 367 385 17 1452 86 12 346 Cuts at: 5009 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 372 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4462 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2270 487 Size: 683 56 1044 3231 Fragments arranged by size: 3231 1044 683 56 BtsI GCAGTG_nn' Cuts at: 2530 3324 3344 2530 Size: 794 20 4200 Fragments arranged by size: 4200 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5014 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4979 DraI TTT'AAA Cuts at: 2318 2318 Size: 5014 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4021 809 Size: 507 2705 1802 Fragments arranged by size: 2705 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2461 3350 4979 Size: 54 1425 50 275 18 631 889 1629 Cuts at: 4979 8 Size: 43 Fragments arranged by size: 1629 1425 889 631 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2461 1537 Size: 275 649 4090 Fragments arranged by size: 4090 649 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2273 2944 1353 Size: 920 671 3423 Fragments arranged by size: 3423 920 671 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2040 3601 3975 4121 387 Size: 1064 138 451 1561 374 146 1280 Fragments arranged by size: 1561 1280 1064 451 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2297 3077 3674 4461 4729 1239 Size: 393 569 96 780 597 787 268 1524 Fragments arranged by size: 1524 787 780 597 569 393 268 96 EcoO109I rG'GnC_Cy Cuts at: 2068 2068 Size: 5014 EcoRI G'AATT_C Cuts at: 2323 2701 2323 Size: 378 4636 FspI TGC'GCA Cuts at: 3520 3520 Size: 5014 HaeII r_GCGC'y Cuts at: 1678 2052 2116 4161 1678 Size: 374 64 2045 2531 Fragments arranged by size: 2531 2045 374 64 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2067 2099 3655 3687 3729 Size: 32 135 32 495 32 1556 32 42 Cuts at: 3729 3761 1373 Size: 32 2626 Fragments arranged by size: 2626 1556 495 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2358 2604 669 Size: 988 281 420 246 3079 Fragments arranged by size: 3079 988 420 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5014 HpaI GTT'AAC Cuts at: 2604 2604 Size: 5014 KasI G'GCGC_C Cuts at: 2112 2112 Size: 5014 KpnI G_GTAC'C Cuts at: 2339 2339 Size: 5014 MfeI C'AATT_G Cuts at: 2613 2613 Size: 5014 MluI A'CGCG_T Cuts at: 4890 4890 Size: 5014 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4128 4312 1305 Size: 2823 184 2007 Fragments arranged by size: 2823 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3192 Fragments arranged by size: 3192 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2972 3331 3490 518 Size: 648 1806 359 159 2042 Fragments arranged by size: 2042 1806 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2430 3107 4255 4500 1606 Size: 824 677 1148 245 2120 Fragments arranged by size: 2120 1148 824 677 245 NarI GG'CG_CC Cuts at: 2113 2113 Size: 5014 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4462 NdeI CA'TA_TG Cuts at: 387 387 Size: 5014 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5014 NotI GC'GGCC_GC Cuts at: 2461 2461 Size: 5014 NspI r_CATG'y Cuts at: 1132 1229 2372 3917 1132 Size: 97 1143 1545 2229 Fragments arranged by size: 2229 1545 1143 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5014 PciI A'CATG_T Cuts at: 3913 3913 Size: 5014 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5014 PmeI GTTT'AAAC Cuts at: 2318 2318 Size: 5014 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3083 3115 4596 4628 3083 Size: 32 1481 32 3469 Fragments arranged by size: 3469 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2068 2068 Size: 5014 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5014 PsiI TTA'TAA Cuts at: 2584 2584 Size: 5014 PstI C_TGCA'G Cuts at: 830 2366 830 Size: 1536 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5014 SalI G'TCGA_C Cuts at: 1936 2356 1936 Size: 420 4594 SbfI CC_TGCA'GG Cuts at: 2366 2366 Size: 5014 ScaI AGT'ACT Cuts at: 3262 4974 3262 Size: 1712 3302 SfcI C'TryA_G Cuts at: 826 1045 2362 3497 4178 4369 826 Size: 219 1317 1135 681 191 1471 Fragments arranged by size: 1471 1317 1135 681 219 191 SfoI GGC'GCC Cuts at: 2114 2114 Size: 5014 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5014 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5014 SmaI CCC'GGG Cuts at: 1872 2341 1872 Size: 469 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3118 4019 4281 820 Size: 197 926 1175 901 262 1553 Fragments arranged by size: 1553 1175 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2805 493 Size: 2312 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5014 SphI G_CATG'C Cuts at: 1132 2372 1132 Size: 1240 3774 SspI AAT'ATT Cuts at: 5 52 2938 5 Size: 47 2886 2081 Fragments arranged by size: 2886 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5014 StyI C'CwwG_G Cuts at: 513 1065 2453 513 Size: 552 1388 3074 Fragments arranged by size: 3074 1388 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3041 3058 3211 3396 3841 325 Size: 1255 1461 17 153 185 445 1498 Fragments arranged by size: 1498 1461 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3260 4972 96 Size: 274 80 33 51 1443 1283 1712 138 Fragments arranged by size: 1712 1443 1283 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4974 5006 4974 Size: 32 4982 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5014 XbaI T'CTAG_A Cuts at: 2350 2350 Size: 5014 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5014 XmaI C'CCGG_G Cuts at: 1870 2339 1870 Size: 469 4545 XmnI GAAnn'nnTTC Cuts at: 3143 3143 Size: 5014 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2822 276 Size: 53 83 186 936 1288 2468 Fragments arranged by size: 2468 1288 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI