Restriction map for
pFN21AB7292
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# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Acc65I AccIII AcsI AflII
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AvrII BamHI BbvCI
BfrI BlnI BseAI BsiWI BspDI
BspEI BspLU11I BssHII Bst1107I BstBI
BstZ17I ClaI CpoI EcoRI FseI
HincII HindII HpaI KpnI MluI
MroI NaeI NgoMIV NotI NruI
NspV PacI PaeR7I PmeI PshBI
PvuI RsrII SalI ScaI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 2224 GTCTAC GT^[AC][GT]AC
AclI 659 AACGTT AA^CGTT
AgeI 413 ACCGGT A^CCGGT
AhdI 427 GACGGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 603 CCTGAGG CC^T[ACGT]AGG
Aor51HI 104 AGCGCT AGC^GCT
AspEI 427 GACGGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 821 TGCGCA TGC^GCA
BbeI 911 GGCGCC GGCGC^C
BciVI 3529 GTATCC GTATCC(6/5)
BglI 1301 GCCACGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3243 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3243 GCTGAGC GC^T[ACGT]AGC
BsaBI 2502 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 413 ACCGGT [AT]^CCGG[AT]
BsiEI 173 CGGCCG CG[GA][CT]^CG
BsmI 2652 GAATGC GAATGC(1/-1)
BsrFI 413 ACCGGT [GA]^CCGG[CT]
BstEII 2163 GGTAACC G^GT[ACGT]ACC
BstPI 2163 GGTAACC G^GT[ACGT]ACC
Bsu36I 603 CCTGAGG CC^T[ACGT]AGG
CelII 3243 GCTGAGC GC^T[ACGT]AGC
Cfr10I 413 ACCGGT [GA]^CCGG[CT]
DraI 1168 TTTAAA TTT^AAA
DraIII 3145 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 173 CGGCCG C^GGCCG
Eam1105I 427 GACGGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 173 CGGCCG C^GGCCG
Eco47III 104 AGCGCT AGC^GCT
Eco52I 173 CGGCCG C^GGCCG
Eco81I 603 CCTGAGG CC^T[ACGT]AGG
EcoNI 3024 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2163 GGTAACC G^GT[ACGT]ACC
EcoRV 1438 GATATC GAT^ATC
EcoT22I 2572 ATGCAT ATGCA^T
FspI 821 TGCGCA TGC^GCA
KasI 911 GGCGCC G^GCGCC
KspI 3278 CCGCGG CCGC^GG
MamI 2502 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 666 CAATTG C^AATTG
MunI 666 CAATTG C^AATTG
NarI 911 GGCGCC GG^CGCC
NdeI 2722 CATATG CA^TATG
NheI 2394 GCTAGC G^CTAGC
NsiI 2572 ATGCAT ATGCA^T
PinAI 413 ACCGGT A^CCGGT
PpuMI 3386 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 672 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 659 AACGTT AA^CGTT
PvuII 3241 CAGCTG CAG^CTG
SacI 1885 GAGCTC GAGCT^C
SacII 3278 CCGCGG CCGC^GG
SbfI 3390 CCTGCAGG CCTGCA^GG
SexAI 2691 ACCAGGT A^CC[AT]GGT
SfiI 1300 GGCCACGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 911 GGCGCC GGC^GCC
SmaI 3172 CCCGGG CCC^GGG
SmlI 1756 CTTGAG C^T[CT][GA]AG
SphI 2570 GCATGC GCATG^C
Sse8387I 3390 CCTGCAGG CCTGCA^GG
StuI 753 AGGCCT AGG^CCT
XmaCI 3172 CCCGGG C^CCGGG
XmaI 3172 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 251 ACACGT A^C[GA][CT]GT
3326 ACGTGT
AspI 274 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
649 GACATAGTC
BalI 113 TGGCCA TGG^CCA
1299 TGGCCA
BbrPI 1303 CACGTG CAC^GTG
2746 CACGTG
BbsI 2751 GAAGAC GAAGAC(2/6)
3361 GAAGAC
BcgI 1016 CGATAAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
88 GCAGGAGCGTCG
BclI 2120 TGATCA T^GATCA
2171 TGATCA
BglII 1719 AGATCT A^GATCT
2075 AGATCT
BpuAI 2751 GAAGAC GAAGAC(2/6)
3361 GAAGAC
BsaI 2995 GAGACC GGTCTC(1/5)
3267 GGTCTC
BssSI 1086 CACGAG CACGAG(-5/-1)
3337 CACGAG
BstXI 2243 CCAGCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2811 CCAAGGCTCTGG
DraII 3159 AGGCCCC [GA]G^G[ACGT]CC[CT]
3386 AGGACCT
DrdI 124 GACGGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3435 GACGCCTCCGTC
EcoO109I 3159 AGGCCCC [GA]G^G[ACGT]CC[CT]
3386 AGGACCT
FbaI 2120 TGATCA T^GATCA
2171 TGATCA
HindIII 1194 AAGCTT A^AGCTT
1688 AAGCTT
MluNI 113 TGGCCA TGG^CCA
1299 TGGCCA
MscI 113 TGGCCA TGG^CCA
1299 TGGCCA
NcoI 1256 CCATGG C^CATGG
2924 CCATGG
PflFI 274 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
649 GACATAGTC
PmaCI 1303 CACGTG CAC^GTG
2746 CACGTG
PmlI 1303 CACGTG CAC^GTG
2746 CACGTG
Tth111I 274 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
649 GACATAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaLI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspHI BspMI
BsrBI BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII