(Circular) (Six-base) MAPSORT of: pFN21AB7292.seq Check: 4541 from: 1 to: 8287 pFN21AB7292 8287 bp With 182 enzymes: SgfI * October 14, 2009 13:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3695 5235 3695 Size: 1540 6747 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6097 278 Size: 53 83 186 936 4561 2468 Fragments arranged by size: 4561 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4230 5630 1937 Size: 2293 1400 4594 Fragments arranged by size: 4594 2293 1400 Acc65I G'GTAC_C Cuts at: 5608 5608 Size: 8287 AclI AA'CG_TT Cuts at: 1563 2665 6414 6787 1563 Size: 1102 3749 373 3063 Fragments arranged by size: 3749 3063 1102 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3486 5110 6350 7734 8002 1632 Size: 1854 1624 1240 1384 268 1917 Fragments arranged by size: 1917 1854 1624 1384 1240 268 AfeI AGC'GCT Cuts at: 2111 2111 Size: 8287 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8090 AflIII A'CryG_T Cuts at: 1203 2256 5331 7186 8163 1203 Size: 1053 3075 1855 977 1327 Fragments arranged by size: 3075 1855 1327 1053 977 AgeI A'CCGG_T Cuts at: 2418 7118 2418 Size: 4700 3587 AhdI GACnn_n'nnGTC Cuts at: 2437 7016 2437 Size: 4579 3708 AleI CACnn'nnGTG Cuts at: 1166 3866 1166 Size: 2700 5587 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2906 3368 5501 1184 Size: 1722 462 2133 3970 Fragments arranged by size: 3970 2133 1722 462 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2234 2266 5013 5045 2234 Size: 32 2747 32 5476 Fragments arranged by size: 5476 2747 32 32 AlwNI CAG_nnn'CTG Cuts at: 2524 5227 5236 6030 7602 2524 Size: 2703 9 794 1572 3209 Fragments arranged by size: 3209 2703 1572 794 9 ApaLI G'TGCA_C Cuts at: 3488 4748 4937 6344 7500 3488 Size: 1260 189 1407 1156 4275 Fragments arranged by size: 4275 1407 1260 1156 189 ApoI r'AATT_y Cuts at: 1493 5596 5822 5974 8083 1493 Size: 4103 226 152 2109 1697 Fragments arranged by size: 4103 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6841 160 Size: 872 5809 1606 Fragments arranged by size: 5809 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8287 AvaI C'yCGr_G Cuts at: 1870 1943 2192 5177 5473 5612 1870 Size: 73 249 2985 296 139 4545 Fragments arranged by size: 4545 2985 296 249 139 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4201 4234 5113 5146 4201 Size: 33 879 33 7342 Fragments arranged by size: 7342 879 33 33 BamHI G'GATC_C Cuts at: 5617 5984 5617 Size: 367 7920 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2916 4711 4734 5485 5608 Size: 325 217 627 1129 1795 23 751 123 Cuts at: 5608 7063 618 Size: 1455 1842 Fragments arranged by size: 1842 1795 1455 1129 751 627 325 217 123 23 BanII G_rGCy'C Cuts at: 1391 2016 2152 3744 3894 4120 5606 1391 Size: 625 136 1592 150 226 1486 4072 Fragments arranged by size: 4072 1592 1486 625 226 150 136 BbeI G_GCGC'C Cuts at: 2920 2920 Size: 8287 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4763 5373 928 Size: 971 2864 610 3842 Fragments arranged by size: 3842 2864 971 610 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2082 2116 3010 3044 6478 6512 1903 Size: 34 145 34 894 34 3434 34 3678 Fragments arranged by size: 3678 3434 894 145 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5545 6179 7389 5545 Size: 634 1210 6443 Fragments arranged by size: 6443 1210 634 BclI T'GATC_A Cuts at: 1548 4125 4176 1548 Size: 2577 51 5659 Fragments arranged by size: 5659 2577 51 BfrBI ATG'CAT Cuts at: 4579 4579 Size: 8287 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3312 6898 136 Size: 107 122 71 631 605 1640 3586 1525 Fragments arranged by size: 3586 1640 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3724 4080 8282 3724 Size: 356 4202 3729 Fragments arranged by size: 4202 3729 356 BlpI GC'TnA_GC Cuts at: 5249 5704 5249 Size: 455 7832 Bme1580I G_kGCm'C Cuts at: 1790 2193 3492 3938 4338 4413 4716 4752 4941 Size: 403 1299 446 400 75 303 36 189 Cuts at: 4941 5490 6348 7504 1790 Size: 549 858 1156 2573 Fragments arranged by size: 2573 1299 1156 858 549 446 403 400 303 189 75 36 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8287 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 6018 6976 442 Size: 416 53 83 399 24 4601 958 1753 Fragments arranged by size: 4601 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4403 1056 Size: 3347 4940 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5290 5290 Size: 8287 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2479 2513 3021 3414 3865 3965 5039 5090 Size: 1240 34 508 393 451 100 1074 51 Cuts at: 5090 5306 6947 1239 Size: 216 1641 2579 Fragments arranged by size: 2579 1641 1240 1074 508 451 393 216 100 51 34 Bpu10I CC'TnA_GC Cuts at: 2903 4119 2903 Size: 1216 7071 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3782 6412 7277 7575 3782 Size: 2630 865 298 4494 Fragments arranged by size: 4494 2630 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4994 5278 6950 882 Size: 617 3495 284 1672 2219 Fragments arranged by size: 3495 2219 1672 617 284 BsaAI yAC'GTr Cuts at: 493 1204 3310 4753 5332 6078 493 Size: 711 2106 1443 579 746 2702 Fragments arranged by size: 2702 2106 1443 746 711 579 BsaBI GATnn'nnATC Cuts at: 4511 8281 4511 Size: 3770 4517 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2917 2925 5441 6094 Size: 53 83 186 936 1384 8 2516 653 Cuts at: 6094 6476 7111 275 Size: 382 635 1451 Fragments arranged by size: 2516 1451 1384 936 653 635 382 186 83 53 8 BsaWI w'CCGG_w Cuts at: 2418 6720 7118 7392 7539 7950 2418 Size: 4302 398 274 147 411 2755 Fragments arranged by size: 4302 2755 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2234 2264 3387 3392 3417 Size: 30 165 30 831 30 1123 5 25 Cuts at: 3417 3422 4695 4725 5013 5043 5318 5348 1178 Size: 5 1273 30 288 30 275 30 4117 Fragments arranged by size: 4117 1273 1123 831 288 275 165 30 30 30 30 30 30 25 5 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3413 3994 4280 4892 4895 4898 5012 5040 Size: 2227 581 286 612 3 3 114 28 Cuts at: 5040 5279 5309 5396 5399 5402 1186 Size: 239 30 87 3 3 4071 Fragments arranged by size: 4071 2227 612 581 286 239 114 87 30 28 3 3 3 3 BseYI C'CCAG_C Cuts at: 2032 2798 4893 5489 7490 7986 2032 Size: 766 2095 596 2001 496 2333 Fragments arranged by size: 2333 2095 2001 766 596 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2537 3169 3595 4069 5503 1163 Size: 573 801 632 426 474 1434 3947 Fragments arranged by size: 3947 1434 801 632 573 474 426 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2181 5737 6498 7526 1540 Size: 275 186 180 3556 761 1028 2301 Fragments arranged by size: 3556 2301 1028 761 275 186 180 BsiHKAI G_wGCw'C Cuts at: 3492 3894 4752 4941 5255 5343 5517 6348 6433 Size: 402 858 189 314 88 174 831 85 Cuts at: 6433 7504 3492 Size: 1071 4275 Fragments arranged by size: 4275 1071 858 831 402 314 189 174 88 85 BsmI GAATG_Cn' Cuts at: 4663 5798 5891 4663 Size: 1135 93 7059 Fragments arranged by size: 7059 1135 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2109 3417 4760 2109 Size: 1308 1343 5636 Fragments arranged by size: 5636 1343 1308 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2152 2193 3492 3744 3894 3938 Size: 399 226 136 41 1299 252 150 44 Cuts at: 3938 4120 4338 4413 4716 4752 4941 5255 5343 Size: 182 218 75 303 36 189 314 88 Cuts at: 5343 5490 5517 5606 6348 6433 7504 1391 Size: 147 27 89 742 85 1071 2174 Fragments arranged by size: 2174 1299 1071 742 399 314 303 252 226 218 189 182 150 147 136 89 88 85 75 44 41 36 27 BspEI T'CCGG_A Cuts at: 7950 7950 Size: 8287 BspHI T'CATG_A Cuts at: 3447 4104 4296 6176 7906 3447 Size: 657 192 1880 1730 3828 Fragments arranged by size: 3828 1880 1730 657 192 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2532 3695 5235 5403 5642 844 Size: 1688 1163 1540 168 239 3489 Fragments arranged by size: 3489 1688 1540 1163 239 168 BsrBI CCG'CTC Cuts at: 1394 1823 2721 4608 5347 6174 1394 Size: 429 898 1887 739 827 3507 Fragments arranged by size: 3507 1887 898 827 739 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2469 3199 3948 4128 6782 6956 65 Size: 1183 1221 730 749 180 2654 174 1396 Fragments arranged by size: 2654 1396 1221 1183 749 730 180 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2418 6931 7118 1700 Size: 110 608 4513 187 2869 Fragments arranged by size: 4513 2869 608 187 110 BsrGI T'GTAC_A Cuts at: 96 2595 4377 4509 96 Size: 2499 1782 132 3874 Fragments arranged by size: 3874 2499 1782 132 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8287 BssSI C'ACGA_G Cuts at: 3091 5342 6347 7359 3091 Size: 2251 1005 1012 4019 Fragments arranged by size: 4019 2251 1012 1005 BstAPI GCAn_nnn'nTGC Cuts at: 794 2524 3283 3651 4658 5227 5236 794 Size: 1730 759 368 1007 569 9 3845 Fragments arranged by size: 3845 1730 1007 759 569 368 9 BstBI TT'CG_AA Cuts at: 7091 7091 Size: 8287 BstEII G'GTnAC_C Cuts at: 1184 4168 1184 Size: 2984 5303 BstXI CCAn_nnnn'nTGG Cuts at: 4255 4823 4255 Size: 568 7719 BstYI r'GATC_y Cuts at: 1972 2316 3724 4080 5617 5984 6369 6386 7838 Size: 344 1408 356 1537 367 385 17 1452 Cuts at: 7838 7924 7936 8282 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1537 1452 1408 385 367 356 346 344 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2609 2609 Size: 8287 BtgI C'CryG_G Cuts at: 513 1065 2004 2343 3261 4929 5283 513 Size: 552 939 339 918 1668 354 3517 Fragments arranged by size: 3517 1668 939 918 552 354 339 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3336 3778 4025 4602 487 Size: 683 56 2110 442 247 577 4172 Fragments arranged by size: 4172 2110 683 577 442 247 56 BtsI GCAGTG_nn' Cuts at: 2317 3583 4464 4552 5803 6597 6617 2317 Size: 1266 881 88 1251 794 20 3987 Fragments arranged by size: 3987 1266 1251 881 794 88 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8287 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8252 DraI TTT'AAA Cuts at: 3175 5591 3175 Size: 2416 5871 DraIII CAC_nnn'GTG Cuts at: 5155 5155 Size: 8287 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2135 5446 7294 809 Size: 507 819 3311 1848 1802 Fragments arranged by size: 3311 1848 1802 819 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2118 2178 2434 Size: 54 1425 50 275 18 288 60 256 Cuts at: 2434 3304 5016 5403 5734 6623 8252 8 Size: 870 1712 387 331 889 1629 43 Fragments arranged by size: 1712 1629 1425 889 870 387 331 288 275 256 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2178 5734 1537 Size: 275 366 3556 4090 Fragments arranged by size: 4090 3556 366 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2159 2344 2540 2801 5015 5373 5581 6217 Size: 806 185 196 261 2214 358 208 636 Cuts at: 6217 1353 Size: 3423 Fragments arranged by size: 3423 2214 806 636 358 261 208 196 185 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4959 6874 7248 7394 387 Size: 1064 138 3370 1915 374 146 1280 Fragments arranged by size: 3370 1915 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3892 3892 Size: 8287 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2479 2513 3021 3414 3486 3865 3965 Size: 393 847 34 508 393 72 379 100 Cuts at: 3965 5039 5090 5110 5306 6350 6947 7734 8002 Size: 1074 51 20 196 1044 597 787 268 Cuts at: 8002 1239 Size: 1524 Fragments arranged by size: 1524 1074 1044 847 787 597 508 393 393 379 268 196 100 72 51 34 20 EcoNI CCTnn'n_nnAGG Cuts at: 5033 5033 Size: 8287 EcoO109I rG'GnC_Cy Cuts at: 5165 5392 5165 Size: 227 8060 EcoRI G'AATT_C Cuts at: 5596 5974 5596 Size: 378 7909 EcoRV GAT'ATC Cuts at: 3445 3445 Size: 8287 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4250 4250 Size: 8287 FspI TGC'GCA Cuts at: 2828 6793 2828 Size: 3965 4322 HaeII r_GCGC'y Cuts at: 1678 2113 2910 2920 3988 7434 1678 Size: 435 797 10 1068 3446 2531 Fragments arranged by size: 3446 2531 1068 797 435 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2124 2156 2423 2455 2850 Size: 32 135 32 552 32 267 32 395 Cuts at: 2850 2882 2964 2996 3032 3064 4278 4310 6928 Size: 32 82 32 36 32 1214 32 2618 Cuts at: 6928 6960 7002 7034 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2618 1214 552 395 267 135 82 42 36 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5631 5877 669 Size: 988 281 3693 246 3079 Fragments arranged by size: 3693 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3199 3693 748 Size: 2451 494 5342 Fragments arranged by size: 5342 2451 494 HpaI GTT'AAC Cuts at: 5877 5877 Size: 8287 KasI G'GCGC_C Cuts at: 2916 2916 Size: 8287 KpnI G_GTAC'C Cuts at: 5612 5612 Size: 8287 MfeI C'AATT_G Cuts at: 2671 5886 2671 Size: 3215 5072 MluI A'CGCG_T Cuts at: 8163 8163 Size: 8287 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2241 3475 4635 7401 7585 1305 Size: 936 1234 1160 2766 184 2007 Fragments arranged by size: 2766 2007 1234 1160 936 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2120 3306 10 Size: 54 1425 343 288 1186 4991 Fragments arranged by size: 4991 1425 1186 343 288 54 MslI CAynn'nnrTG Cuts at: 518 1166 2513 2888 3749 3866 4934 6245 6604 Size: 648 1347 375 861 117 1068 1311 359 Cuts at: 6604 6763 518 Size: 159 2042 Fragments arranged by size: 2042 1347 1311 1068 861 648 375 359 159 117 MspA1I CmG'CkG Cuts at: 1606 5242 5248 5285 5703 6380 7528 7773 1606 Size: 3636 6 37 418 677 1148 245 2120 Fragments arranged by size: 3636 2120 1148 677 418 245 37 6 NarI GG'CG_CC Cuts at: 2917 2917 Size: 8287 NcoI C'CATG_G Cuts at: 513 1065 2004 3261 4929 513 Size: 552 939 1257 1668 3871 Fragments arranged by size: 3871 1668 1257 939 552 NdeI CA'TA_TG Cuts at: 387 4728 387 Size: 4341 3946 NheI G'CTAG_C Cuts at: 1052 4399 1052 Size: 3347 4940 NotI GC'GGCC_GC Cuts at: 5734 5734 Size: 8287 NsiI A_TGCA'T Cuts at: 4581 4581 Size: 8287 NspI r_CATG'y Cuts at: 1132 1229 3286 4495 4579 4583 5645 7190 1132 Size: 97 2057 1209 84 4 1062 1545 2229 Fragments arranged by size: 2229 2057 1545 1209 1062 97 84 4 PasI CC'CwG_GG Cuts at: 1348 2305 1348 Size: 957 7330 PciI A'CATG_T Cuts at: 7186 7186 Size: 8287 PflMI CCAn_nnn'nTGG Cuts at: 1108 2239 2260 5113 1108 Size: 1131 21 2853 4282 Fragments arranged by size: 4282 2853 1131 21 PfoI T'CCnGG_A Cuts at: 2319 3145 3219 3430 5139 5322 2319 Size: 826 74 211 1709 183 5284 Fragments arranged by size: 5284 1709 826 211 183 74 PmeI GTTT'AAAC Cuts at: 5591 5591 Size: 8287 PmlI CAC'GTG Cuts at: 3310 4753 3310 Size: 1443 6844 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2234 2266 5013 5045 6356 6388 7869 7901 2234 Size: 32 2747 32 1311 32 1481 32 2620 Fragments arranged by size: 2747 2620 1481 1311 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5392 5392 Size: 8287 PshAI GACnn'nnGTC Cuts at: 1613 2681 1613 Size: 1068 7219 PsiI TTA'TAA Cuts at: 5857 5857 Size: 8287 PstI C_TGCA'G Cuts at: 830 2692 3033 3279 3459 5232 5247 5400 5639 Size: 1862 341 246 180 1773 15 153 239 Cuts at: 5639 830 Size: 3478 Fragments arranged by size: 3478 1862 1773 341 246 239 180 153 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8287 PvuII CAG'CTG Cuts at: 5248 5248 Size: 8287 SacI G_AGCT'C Cuts at: 3894 3894 Size: 8287 SacII CC_GC'GG Cuts at: 5286 5286 Size: 8287 SalI G'TCGA_C Cuts at: 1936 5629 1936 Size: 3693 4594 SapI GCTCTTCn'nnn_ Cuts at: 2540 2801 5581 2540 Size: 261 2780 5246 Fragments arranged by size: 5246 2780 261 SbfI CC_TGCA'GG Cuts at: 5400 5639 5400 Size: 239 8048 ScaI AGT'ACT Cuts at: 6535 8247 6535 Size: 1712 6575 SexAI A'CCwGG_T Cuts at: 4696 4696 Size: 8287 SfcI C'TryA_G Cuts at: 826 1045 2502 2688 2788 3029 3275 3455 5228 Size: 219 1457 186 100 241 246 180 1773 Cuts at: 5228 5243 5396 5635 6770 7451 7642 826 Size: 15 153 239 1135 681 191 1471 Fragments arranged by size: 1773 1471 1457 1135 681 246 241 239 219 191 186 180 153 100 15 SfiI GGCCn_nnn'nGGCC Cuts at: 3312 3312 Size: 8287 SfoI GGC'GCC Cuts at: 2918 2918 Size: 8287 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8287 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8287 SmaI CCC'GGG Cuts at: 1872 5179 5614 1872 Size: 3307 435 4545 Fragments arranged by size: 4545 3307 435 SmlI C'TyrA_G Cuts at: 820 1017 1943 3761 6391 7292 7554 820 Size: 197 926 1818 2630 901 262 1553 Fragments arranged by size: 2630 1818 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6078 493 Size: 5585 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8287 SphI G_CATG'C Cuts at: 1132 4579 5645 1132 Size: 3447 1066 3774 Fragments arranged by size: 3774 3447 1066 SspI AAT'ATT Cuts at: 5 52 6211 5 Size: 47 6159 2081 Fragments arranged by size: 6159 2081 47 StuI AGG'CCT Cuts at: 1517 2760 1517 Size: 1243 7044 StyI C'CwwG_G Cuts at: 513 1065 2004 2850 2920 3261 3922 4716 4816 Size: 552 939 846 70 341 661 794 100 Cuts at: 4816 4929 5034 5726 513 Size: 113 105 692 3074 Fragments arranged by size: 3074 939 846 794 692 661 552 341 113 105 100 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2474 2832 4247 6314 6331 6484 6669 Size: 1255 894 358 1415 2067 17 153 185 Cuts at: 6669 7114 325 Size: 445 1498 Fragments arranged by size: 2067 1498 1415 1255 894 445 358 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2595 3459 3847 Size: 274 80 33 51 1443 618 864 388 Cuts at: 3847 4377 4509 6533 8245 96 Size: 530 132 2024 1712 138 Fragments arranged by size: 2024 1712 1443 864 618 530 388 274 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3082 3114 3387 3419 4695 4727 8247 8279 3082 Size: 32 273 32 1276 32 3520 32 3090 Fragments arranged by size: 3520 3090 1276 273 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2282 2657 1316 Size: 966 375 6946 Fragments arranged by size: 6946 966 375 XbaI T'CTAG_A Cuts at: 5623 5623 Size: 8287 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2302 2857 3259 2302 Size: 555 402 7330 Fragments arranged by size: 7330 555 402 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8287 XmaI C'CCGG_G Cuts at: 1870 5177 5612 1870 Size: 3307 435 4545 Fragments arranged by size: 4545 3307 435 XmnI GAAnn'nnTTC Cuts at: 6416 6416 Size: 8287 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6095 276 Size: 53 83 186 936 4561 2468 Fragments arranged by size: 4561 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BbvCI BsiWI BstZ17I FseI FspAI NaeI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SanDI SrfI SwaI