Restriction map for
pFN21AE0438
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbsI BbvCI BcgI
BclI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspLU11I BsrBI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AatII 739 GACGTC GACGT^C
AccI 600 GTCTAC GT^[AC][GT]AC
AccII 63 CGCG CG^CG
AcsI 580 GAATTC [GA]^AATT[CT]
AgeI 6 ACCGGT A^CCGGT
AlwNI 791 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 565 CCTGAGG CC^T[ACGT]AGG
ApaI 152 GGGCCC GGGCC^C
ApoI 580 GAATTC [GA]^AATT[CT]
AspHI 459 GAGCAC G[AT]GC[AT]^C
AvaI 71 CCCGGG C^[CT]CG[GA]G
AvaII 413 GGACC G^G[AT]CC
BalI 773 TGGCCA TGG^CCA
BanI 551 GGCGCC G^G[CT][GA]CC
BbeI 551 GGCGCC GGCGC^C
BbrPI 184 CACGTG CAC^GTG
BciVI 41 GGATAC GTATCC(6/5)
BsaAI 184 CACGTG [CT]AC^GT[GA]
BsaI 3 GAGACC GGTCTC(1/5)
BsaWI 6 ACCGGT [AT]^CCGG[AT]
BseRI 172 CTCCTC GAGGAG(10/8)
BsiHKAI 459 GAGCAC G[AT]GC[AT]^C
BsoBI 71 CCCGGG C^[CT]CG[GA]G
Bsp1407I 542 TGTACA T^GTACA
BspHI 17 TCATGA T^CATGA
BsrDI 501 CATTGC GCAATG(2/0)
BsrGI 542 TGTACA T^GTACA
BssSI 417 CTCGTG CACGAG(-5/-1)
BstUI 63 CGCG CG^CG
BstXI 239 CCATGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 565 CCTGAGG CC^T[ACGT]AGG
DraII 151 GGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 156 CCACGG C^C[GA][CT]GG
EaeI 773 TGGCCA [CT]^GGCC[GA]
Eco81I 565 CCTGAGG CC^T[ACGT]AGG
EcoNI 759 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 151 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 580 GAATTC G^AATTC
HaeII 551 GGCGCC [GA]GCGC^[CT]
HgaI 510 GCGTC GACGC(5/10)
HphI 32 TCACC GGTGA(8/7)
KasI 551 GGCGCC G^GCGCC
MluNI 773 TGGCCA TGG^CCA
MscI 773 TGGCCA TGG^CCA
MseI 652 TTAA T^TAA
MvnI 63 CGCG CG^CG
NarI 551 GGCGCC GG^CGCC
PflMI 765 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 6 ACCGGT A^CCGGT
PmaCI 184 CACGTG CAC^GTG
PmlI 184 CACGTG CAC^GTG
PstI 209 CTGCAG CTGCA^G
RcaI 17 TCATGA T^CATGA
SfcI 209 CTGCAG C^T[GA][CT]AG
SfoI 551 GGCGCC GGC^GCC
SmaI 71 CCCGGG CCC^GGG
SmlI 976 CTCAAG C^T[CT][GA]AG
SspBI 542 TGTACA T^GTACA
TfiI 867 GATTC G^A[AT]TC
Tru9I 652 TTAA T^TAA
Tsp509I 581 AATT ^AATT
Van91I 765 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 911 CCATGTCCTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 71 CCCGGG C^CCGGG
XmaI 71 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AfaI 543 GTAC GT^AC
762 GTAC
Asp700I 21 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
516 GAAACCATTC
BamHI 197 GGATCC G^GATCC
290 GGATCC
BcnI 71 CCCGG CC^[GC]GG
72 CCGGG
BfaI 133 CTAG C^TAG
968 CTAG
BglI 70 GCCCGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
426 GCCCCTCTGGC
BsmAI 3 GAGAC GTCTC(1/5)
346 GTCTC
BsmFI 101 GGGAC GGGAC(10/14)
669 GGGAC
BspMI 364 ACCTGC ACCTGC(4/8)
657 ACCTGC
BsrFI 6 ACCGGT [GA]^CCGG[CT]
371 GCCGGT
BsrI 801 ACTGG ACTGG(1/-1)
776 CCAGT
BstYI 197 GGATCC [GA]^GATC[CT]
290 GGATCC
Cfr10I 6 ACCGGT [GA]^CCGG[CT]
371 GCCGGT
DpnII 198 GATC ^GATC
291 GATC
EcoT14I 125 CCAAGG C^C[AT][AT]GG
770 CCTTGG
MaeI 133 CTAG C^TAG
968 CTAG
MaeII 185 ACGT A^CGT
740 ACGT
MaeIII 31 GTCAC ^GT[ACGT]AC
808 GTGAC
MboI 198 GATC ^GATC
291 GATC
MflI 197 GGATCC [GA]^GATC[CT]
290 GGATCC
NciI 71 CCCGG CC^[GC]GG
72 CCGGG
NdeII 198 GATC ^GATC
291 GATC
PvuII 710 CAGCTG CAG^CTG
791 CAGCTG
RsaI 543 GTAC GT^AC
762 GTAC
Sau3AI 198 GATC ^GATC
291 GATC
StuI 562 AGGCCT AGG^CCT
767 AGGCCT
StyI 125 CCAAGG C^C[AT][AT]GG
770 CCTTGG
TaqI 254 TCGA T^CGA
389 TCGA
Tsp45I 31 GTCAC ^GT[GC]AC
808 GTGAC
TthHB8I 254 TCGA T^CGA
389 TCGA
XhoII 197 GGATCC [GA]^GATC[CT]
290 GGATCC
XmnI 21 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
516 GAAACCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcyI AluI AlwI BanII
BbvI BmyI BsaHI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
ItaI MboII MnlI MslI MspA1I
MspI MvaI MwoI NlaIII NlaIV
PleI Sau96I ScrFI SfaNI TseI
TspRI