(Circular) (Six-base) MAPSORT of: pFN21AE0438.seq Check: 1773 from: 1 to: 5689 pFN21AE0438 5689 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2378 2671 2378 Size: 293 5396 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2748 3499 278 Size: 53 83 186 936 1212 751 2468 Fragments arranged by size: 2468 1212 936 751 186 83 53 AccI GT'mk_AC Cuts at: 1937 2606 3032 1937 Size: 669 426 4594 Fragments arranged by size: 4594 669 426 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2879 3752 5136 5404 1632 Size: 1247 873 1384 268 1917 Fragments arranged by size: 1917 1384 1247 873 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 4588 5565 1203 Size: 3385 977 1327 Fragments arranged by size: 3385 1327 977 AgeI A'CCGG_T Cuts at: 2011 4520 2011 Size: 2509 3180 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5689 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5689 AlwNI CAG_nnn'CTG Cuts at: 2801 3432 5004 2801 Size: 631 1572 3486 Fragments arranged by size: 3486 1572 631 ApaI G_GGCC'C Cuts at: 2161 2161 Size: 5689 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2585 2998 3224 3376 5485 1493 Size: 1092 413 226 152 2109 1697 Fragments arranged by size: 2109 1697 1092 413 226 152 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 2076 3014 1870 Size: 73 133 938 4545 Fragments arranged by size: 4545 938 133 73 BamHI G'GATC_C Cuts at: 2202 2295 3019 3386 2202 Size: 93 724 367 4505 Fragments arranged by size: 4505 724 367 93 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2556 3010 4465 618 Size: 325 217 627 769 454 1455 1842 Fragments arranged by size: 1842 1455 769 627 454 325 217 BanII G_rGCy'C Cuts at: 1391 2121 2161 2822 3008 1391 Size: 730 40 661 186 4072 Fragments arranged by size: 4072 730 661 186 40 BbeI G_GCGC'C Cuts at: 2560 2560 Size: 5689 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4718 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3880 3914 1903 Size: 34 1943 34 3678 Fragments arranged by size: 3678 1943 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2040 3581 4791 2040 Size: 1541 1210 2938 Fragments arranged by size: 2938 1541 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5689 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2081 2437 4300 Size: 107 122 71 631 605 409 356 1863 Cuts at: 4300 136 Size: 1525 Fragments arranged by size: 1863 1525 631 605 409 356 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 3106 3106 Size: 5689 Bme1580I G_kGCm'C Cuts at: 1790 2161 3750 4906 1790 Size: 371 1589 1156 2573 Fragments arranged by size: 2573 1589 1156 371 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5689 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3420 4378 442 Size: 416 53 83 399 24 2003 958 1753 Fragments arranged by size: 2003 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4349 1239 Size: 3110 2579 Bpu10I CC'TnA_GC Cuts at: 2613 2613 Size: 5689 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2966 3814 4679 4977 2966 Size: 848 865 298 3678 Fragments arranged by size: 3678 865 848 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2002 4352 882 Size: 617 503 2350 2219 Fragments arranged by size: 2350 2219 617 503 BsaAI yAC'GTr Cuts at: 493 1204 2191 3480 493 Size: 711 987 1289 2702 Fragments arranged by size: 2702 1289 987 711 BsaBI GATnn'nnATC Cuts at: 5683 5683 Size: 5689 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2515 2557 2745 3496 Size: 53 83 186 936 982 42 188 751 Cuts at: 3496 3878 4513 275 Size: 382 635 1451 Fragments arranged by size: 1451 982 936 751 635 382 188 186 83 53 42 BsaWI w'CCGG_w Cuts at: 2011 4122 4520 4794 4941 5352 2011 Size: 2111 398 274 147 411 2348 Fragments arranged by size: 2348 2111 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5464 Fragments arranged by size: 5464 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 1186 Size: 982 4707 BseYI C'CCAG_C Cuts at: 2485 4892 5388 2485 Size: 2407 496 2786 Fragments arranged by size: 2786 2407 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5116 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2468 3750 3835 4906 2468 Size: 1282 85 1071 3251 Fragments arranged by size: 3251 1282 1071 85 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2121 2161 2468 2822 3008 3750 3835 Size: 399 331 40 307 354 186 742 85 Cuts at: 3835 4906 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 354 331 307 186 85 40 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 2022 3578 5308 2022 Size: 1556 1730 2403 Fragments arranged by size: 2403 1730 1556 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2378 2671 3044 844 Size: 1534 293 373 3489 Fragments arranged by size: 3489 1534 373 293 BsrBI CCG'CTC Cuts at: 1394 1823 3576 1394 Size: 429 1753 3507 Fragments arranged by size: 3507 1753 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2505 4184 4358 65 Size: 1183 1257 1679 174 1396 Fragments arranged by size: 1679 1396 1257 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2011 2376 4333 4520 1700 Size: 110 201 365 1957 187 2869 Fragments arranged by size: 2869 1957 365 201 187 110 BsrGI T'GTAC_A Cuts at: 96 2547 96 Size: 2451 3238 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 2422 3749 4761 2422 Size: 1327 1012 3350 Fragments arranged by size: 3350 1327 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5689 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstXI CCAn_nnnn'nTGG Cuts at: 2251 2251 Size: 5689 BstYI r'GATC_y Cuts at: 1972 2202 2295 3019 3386 3771 3788 5240 5326 Size: 230 93 724 367 385 17 1452 86 Cuts at: 5326 5338 5684 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 724 385 367 346 230 93 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2571 2571 Size: 5689 BtgI C'CryG_G Cuts at: 513 1065 2161 513 Size: 552 1096 4041 Fragments arranged by size: 4041 1096 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4950 Fragments arranged by size: 4950 683 56 BtsI GCAGTG_nn' Cuts at: 2108 2828 2896 3205 3999 4019 2108 Size: 720 68 309 794 20 3778 Fragments arranged by size: 3778 794 720 309 68 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2272 2307 529 Size: 35 1708 35 3911 Fragments arranged by size: 3911 1708 35 35 DraI TTT'AAA Cuts at: 2993 2993 Size: 5689 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2778 3136 4025 Size: 54 1425 50 275 18 948 358 889 Cuts at: 4025 5654 8 Size: 1629 43 Fragments arranged by size: 1629 1425 948 889 358 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3619 1353 Size: 2266 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4276 4650 4796 387 Size: 1064 138 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2879 3752 4349 5136 5404 1239 Size: 393 1247 873 597 787 268 1524 Fragments arranged by size: 1524 1247 873 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2768 2768 Size: 5689 EcoO109I rG'GnC_Cy Cuts at: 2157 2157 Size: 5689 EcoRI G'AATT_C Cuts at: 2585 2998 3376 2585 Size: 413 378 4898 Fragments arranged by size: 4898 413 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2710 2710 Size: 5689 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 2560 4836 1678 Size: 882 2276 2531 Fragments arranged by size: 2531 2276 882 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2588 2620 4330 4362 4404 Size: 32 135 32 1016 32 1710 32 42 Cuts at: 4404 4436 1373 Size: 32 2626 Fragments arranged by size: 2626 1710 1016 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3279 669 Size: 988 281 1095 246 3079 Fragments arranged by size: 3079 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5689 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KasI G'GCGC_C Cuts at: 2556 2556 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2208 4803 4987 1305 Size: 903 2595 184 2007 Fragments arranged by size: 2595 2007 903 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2780 10 Size: 54 1425 343 948 2919 Fragments arranged by size: 2919 1425 948 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2249 2642 3647 4006 4165 518 Size: 648 861 222 393 1005 359 159 2042 Fragments arranged by size: 2042 1005 861 648 393 359 222 159 MspA1I CmG'CkG Cuts at: 1606 2582 2717 2798 3105 3782 4930 5175 1606 Size: 976 135 81 307 677 1148 245 2120 Fragments arranged by size: 2120 1148 976 677 307 245 135 81 NarI GG'CG_CC Cuts at: 2557 2557 Size: 5689 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5137 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 3047 4592 1132 Size: 97 1818 1545 2229 Fragments arranged by size: 2229 1818 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5689 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 2776 1108 Size: 1668 4021 PfoI T'CCnGG_A Cuts at: 2205 2205 Size: 5689 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PmlI CAC'GTG Cuts at: 2191 2191 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5689 PsiI TTA'TAA Cuts at: 3259 3259 Size: 5689 PspOMI G'GGCC_C Cuts at: 2157 2157 Size: 5689 PstI C_TGCA'G Cuts at: 830 2218 3041 830 Size: 1388 823 3478 Fragments arranged by size: 3478 1388 823 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 PvuII CAG'CTG Cuts at: 2717 2798 2717 Size: 81 5608 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 3937 5649 3937 Size: 1712 3977 SfcI C'TryA_G Cuts at: 826 1045 2214 3037 4172 4853 5044 826 Size: 219 1169 823 1135 681 191 1471 Fragments arranged by size: 1471 1169 1135 823 681 219 191 SfoI GGC'GCC Cuts at: 2558 2558 Size: 5689 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 2078 3016 1872 Size: 206 938 4545 Fragments arranged by size: 4545 938 206 SmlI C'TyrA_G Cuts at: 820 1017 1943 2981 3793 4694 4956 820 Size: 197 926 1038 812 901 262 1553 Fragments arranged by size: 1553 1038 926 901 812 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SrfI GCCC'GGGC Cuts at: 2078 2078 Size: 5689 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 2569 2774 1517 Size: 1052 205 4432 Fragments arranged by size: 4432 1052 205 StyI C'CwwG_G Cuts at: 513 1065 2130 2775 3128 513 Size: 552 1065 645 353 3074 Fragments arranged by size: 3074 1065 645 552 353 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2912 3716 3733 3886 4071 4516 325 Size: 1255 1332 804 17 153 185 445 1498 Fragments arranged by size: 1498 1332 1255 804 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2547 3935 5647 Size: 274 80 33 51 1443 570 1388 1712 Cuts at: 5647 96 Size: 138 Fragments arranged by size: 1712 1443 1388 570 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2796 2828 5649 5681 2796 Size: 32 2821 32 2804 Fragments arranged by size: 2821 2804 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5689 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2923 2923 Size: 5689 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 2076 3014 1870 Size: 206 938 4545 Fragments arranged by size: 4545 938 206 XmnI GAAnn'nnTTC Cuts at: 2030 2525 3818 2030 Size: 495 1293 3901 Fragments arranged by size: 3901 1293 495 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2746 3497 276 Size: 53 83 186 936 1212 751 2468 Fragments arranged by size: 2468 1212 936 751 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SwaI