Restriction map for
pFN21AE0438
(Linear) (Six-base) MAPSORT of: KIEE0438.seq Check: 8997 from: 1 to: 984
KIEE0438.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 373 666 984
Size: 373 293 318
Fragments arranged by size:
373 318 293
AatII G_ACGT'C
Cuts at: 0 743 984
Size: 743 241
AccI GT'mk_AC
Cuts at: 0 601 984
Size: 601 383
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 984
Size: 874 110
AgeI A'CCGG_T
Cuts at: 0 6 984
Size: 6 978
AlwNI CAG_nnn'CTG
Cuts at: 0 796 984
Size: 796 188
ApaI G_GGCC'C
Cuts at: 0 156 984
Size: 156 828
ApoI r'AATT_y
Cuts at: 0 580 984
Size: 580 404
AvaI C'yCGr_G
Cuts at: 0 71 984
Size: 71 913
BamHI G'GATC_C
Cuts at: 0 197 290 984
Size: 197 93 694
Fragments arranged by size:
694 197 93
BanI G'GyrC_C
Cuts at: 0 551 984
Size: 551 433
BanII G_rGCy'C
Cuts at: 0 116 156 817 984
Size: 116 40 661 167
Fragments arranged by size:
661 167 116 40
BbeI G_GCGC'C
Cuts at: 0 555 984
Size: 555 429
BciVI GTATCCnnnnn_n'
Cuts at: 0 35 984
Size: 35 949
BglI GCCn_nnn'nGGC
Cuts at: 0 76 432 984
Size: 76 356 552
Fragments arranged by size:
552 356 76
Bme1580I G_kGCm'C
Cuts at: 0 156 984
Size: 156 828
Bpu10I CC'TnA_GC
Cuts at: 0 608 984
Size: 608 376
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 984
Size: 961 23
BsaAI yAC'GTr
Cuts at: 0 186 984
Size: 186 798
BsaHI Gr'CG_yC
Cuts at: 0 510 552 740 984
Size: 510 42 188 244
Fragments arranged by size:
510 244 188 42
BsaWI w'CCGG_w
Cuts at: 0 6 984
Size: 6 978
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 984
Size: 163 821
BseYI C'CCAG_C
Cuts at: 0 480 984
Size: 480 504
BsiHKAI G_wGCw'C
Cuts at: 0 463 984
Size: 463 521
Bsp1286I G_dGCh'C
Cuts at: 0 116 156 463 817 984
Size: 116 40 307 354 167
Fragments arranged by size:
354 307 167 116 40
BspHI T'CATG_A
Cuts at: 0 17 984
Size: 17 967
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 373 666 984
Size: 373 293 318
Fragments arranged by size:
373 318 293
BsrDI GCAATG_nn'
Cuts at: 0 500 984
Size: 500 484
BsrFI r'CCGG_y
Cuts at: 0 6 371 984
Size: 6 365 613
Fragments arranged by size:
613 365 6
BsrGI T'GTAC_A
Cuts at: 0 542 984
Size: 542 442
BssSI C'ACGA_G
Cuts at: 0 417 984
Size: 417 567
BstXI CCAn_nnnn'nTGG
Cuts at: 0 246 984
Size: 246 738
BstYI r'GATC_y
Cuts at: 0 197 290 984
Size: 197 93 694
Fragments arranged by size:
694 197 93
Bsu36I CC'TnA_GG
Cuts at: 0 566 984
Size: 566 418
BtgI C'CryG_G
Cuts at: 0 156 984
Size: 156 828
BtsI GCAGTG_nn'
Cuts at: 0 103 823 891 984
Size: 103 720 68 93
Fragments arranged by size:
720 103 93 68
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 267 302 984
Size: 267 35 682
Fragments arranged by size:
682 267 35
EaeI y'GGCC_r
Cuts at: 0 773 984
Size: 773 211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 984
Size: 874 110
EcoNI CCTnn'n_nnAGG
Cuts at: 0 763 984
Size: 763 221
EcoO109I rG'GnC_Cy
Cuts at: 0 152 984
Size: 152 832
EcoRI G'AATT_C
Cuts at: 0 580 984
Size: 580 404
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 705 984
Size: 705 279
HaeII r_GCGC'y
Cuts at: 0 555 984
Size: 555 429
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 583 615 984
Size: 583 32 369
Fragments arranged by size:
583 369 32
KasI G'GCGC_C
Cuts at: 0 551 984
Size: 551 433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 984
Size: 203 781
MscI TGG'CCA
Cuts at: 0 775 984
Size: 775 209
MslI CAynn'nnrTG
Cuts at: 0 22 244 637 984
Size: 22 222 393 347
Fragments arranged by size:
393 347 222 22
MspA1I CmG'CkG
Cuts at: 0 577 712 793 984
Size: 577 135 81 191
Fragments arranged by size:
577 191 135 81
NarI GG'CG_CC
Cuts at: 0 552 984
Size: 552 432
PflMI CCAn_nnn'nTGG
Cuts at: 0 771 984
Size: 771 213
PfoI T'CCnGG_A
Cuts at: 0 200 984
Size: 200 784
PmlI CAC'GTG
Cuts at: 0 186 984
Size: 186 798
PspOMI G'GGCC_C
Cuts at: 0 152 984
Size: 152 832
PstI C_TGCA'G
Cuts at: 0 213 984
Size: 213 771
PvuII CAG'CTG
Cuts at: 0 712 793 984
Size: 712 81 191
Fragments arranged by size:
712 191 81
SfcI C'TryA_G
Cuts at: 0 209 984
Size: 209 775
SfoI GGC'GCC
Cuts at: 0 553 984
Size: 553 431
SmaI CCC'GGG
Cuts at: 0 73 984
Size: 73 911
SmlI C'TyrA_G
Cuts at: 0 976 984
Size: 976 8
SrfI GCCC'GGGC
Cuts at: 0 73 984
Size: 73 911
StuI AGG'CCT
Cuts at: 0 564 769 984
Size: 564 205 215
Fragments arranged by size:
564 215 205
StyI C'CwwG_G
Cuts at: 0 125 770 984
Size: 125 645 214
Fragments arranged by size:
645 214 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 907 984
Size: 907 77
TatI w'GTAC_w
Cuts at: 0 542 984
Size: 542 442
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 791 823 984
Size: 791 32 161
Fragments arranged by size:
791 161 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 918 984
Size: 918 66
XmaI C'CCGG_G
Cuts at: 0 71 984
Size: 71 913
XmnI GAAnn'nnTTC
Cuts at: 0 25 520 984
Size: 25 495 464
Fragments arranged by size:
495 464 25
ZraI GAC'GTC
Cuts at: 0 741 984
Size: 741 243
Enzymes that do cut:
AarI AatII AccI AcuI AgeI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbeI BciVI BglI Bme1580I
Bpu10I BpuEI BsaAI BsaHI BsaWI BseRI BseYI BsiHKAI
Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstXI
BstYI Bsu36I BtgI BtsI CspCI EaeI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I KasI MmeI MscI
MslI MspA1I NarI PflMI PfoI PmlI PspOMI PstI
PvuII SfcI SfoI SmaI SmlI SrfI StuI StyI
TaqII TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BbsI
BbvCI BcgI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI BplI BpmI BsaI BsaBI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BsrBI BssHII BstAPI BstBI
BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI EagI
EarI EciI EcoICRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI Tth111I XbaI XhoI