(Circular) (Six-base) MAPSORT of: pFN21AB7350.seq Check: 2820 from: 1 to: 7942 pFN21AB7350 7942 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2702 2915 3302 4070 4688 2702 Size: 213 387 768 618 5956 Fragments arranged by size: 5956 768 618 387 213 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5752 278 Size: 53 83 186 936 4216 2468 Fragments arranged by size: 4216 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5285 1937 Size: 3348 4594 Acc65I G'GTAC_C Cuts at: 5263 5263 Size: 7942 AclI AA'CG_TT Cuts at: 1563 6069 6442 1563 Size: 4506 373 3063 Fragments arranged by size: 4506 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2623 3674 3796 4078 4871 5144 6005 7389 Size: 991 1051 122 282 793 273 861 1384 Cuts at: 7389 7657 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1051 991 861 793 282 273 268 122 AfeI AGC'GCT Cuts at: 2370 3188 2370 Size: 818 7124 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7745 AflIII A'CryG_T Cuts at: 1203 2907 6841 7818 1203 Size: 1704 3934 977 1327 Fragments arranged by size: 3934 1704 1327 977 AgeI A'CCGG_T Cuts at: 6773 6773 Size: 7942 AhdI GACnn_n'nnGTC Cuts at: 3700 6671 3700 Size: 2971 4971 AleI CACnn'nnGTG Cuts at: 1166 4790 1166 Size: 3624 4318 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4278 4492 1184 Size: 3094 214 4634 Fragments arranged by size: 4634 3094 214 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4400 4432 4400 Size: 32 7910 AlwNI CAG_nnn'CTG Cuts at: 2338 2577 2671 3232 3435 3526 4594 5685 7257 Size: 239 94 561 203 91 1068 1091 1572 Cuts at: 7257 2338 Size: 3023 Fragments arranged by size: 3023 1572 1091 1068 561 239 203 94 91 ApaI G_GGCC'C Cuts at: 2260 4512 2260 Size: 2252 5690 ApaLI G'TGCA_C Cuts at: 5999 7155 5999 Size: 1156 6786 ApoI r'AATT_y Cuts at: 1493 2530 5251 5477 5629 7738 1493 Size: 1037 2721 226 152 2109 1697 Fragments arranged by size: 2721 2109 1697 1037 226 152 AseI AT'TA_AT Cuts at: 160 1032 6496 160 Size: 872 5464 1606 Fragments arranged by size: 5464 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7942 AvaI C'yCGr_G Cuts at: 1870 1943 2654 2711 5267 1870 Size: 73 711 57 2556 4545 Fragments arranged by size: 4545 2556 711 73 57 BamHI G'GATC_C Cuts at: 2715 5272 5639 2715 Size: 2557 367 5018 Fragments arranged by size: 5018 2557 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2814 2989 3514 4203 4487 Size: 325 217 627 1027 175 525 689 284 Cuts at: 4487 5263 6718 618 Size: 776 1455 1842 Fragments arranged by size: 1842 1455 1027 776 689 627 525 325 284 217 175 BanII G_rGCy'C Cuts at: 1391 2260 2828 3119 3652 4512 4716 5261 1391 Size: 869 568 291 533 860 204 545 4072 Fragments arranged by size: 4072 869 860 568 545 533 291 204 BbeI G_GCGC'C Cuts at: 4491 4491 Size: 7942 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2629 4144 5038 928 Size: 971 730 1515 894 3832 Fragments arranged by size: 3832 1515 971 894 730 BbvCI CC'TCA_GC Cuts at: 2455 2894 3507 5168 2455 Size: 439 613 1661 5229 Fragments arranged by size: 5229 1661 613 439 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3736 3770 6133 6167 1903 Size: 34 1799 34 2363 34 3678 Fragments arranged by size: 3678 2363 1799 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5834 7044 5834 Size: 1210 6732 BclI T'GATC_A Cuts at: 1548 4917 1548 Size: 3369 4573 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2553 6553 136 Size: 107 122 71 631 605 881 4000 1525 Fragments arranged by size: 4000 1525 881 631 605 122 107 71 BglII A'GATC_T Cuts at: 4413 7937 4413 Size: 3524 4418 BlpI GC'TnA_GC Cuts at: 2759 3068 3587 5359 2759 Size: 309 519 1772 5342 Fragments arranged by size: 5342 1772 519 309 Bme1580I G_kGCm'C Cuts at: 1790 2260 2994 3791 4512 4685 5231 6003 7159 Size: 470 734 797 721 173 546 772 1156 Cuts at: 7159 1790 Size: 2573 Fragments arranged by size: 2573 1156 797 772 734 721 546 470 173 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7942 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2109 2823 2929 Size: 416 53 83 399 24 692 714 106 Cuts at: 2929 4697 5673 6631 442 Size: 1768 976 958 1753 Fragments arranged by size: 1768 1753 976 958 714 692 416 399 106 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7942 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2784 3175 3325 5011 2784 Size: 391 150 1686 5715 Fragments arranged by size: 5715 1686 391 150 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2331 2413 3133 3141 4488 5021 6602 1239 Size: 1092 82 720 8 1347 533 1581 2579 Fragments arranged by size: 2579 1581 1347 1092 720 533 82 8 Bpu10I CC'TnA_GC Cuts at: 2455 2798 2894 3507 5168 2455 Size: 343 96 613 1661 5229 Fragments arranged by size: 5229 1661 613 343 96 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4311 4710 6067 6932 7230 4311 Size: 399 1357 865 298 5023 Fragments arranged by size: 5023 1357 865 399 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2101 2380 3708 3761 4855 6605 882 Size: 617 602 279 1328 53 1094 1750 2219 Fragments arranged by size: 2219 1750 1328 1094 617 602 279 53 BsaAI yAC'GTr Cuts at: 493 1204 3210 3346 3604 5733 493 Size: 711 2006 136 258 2129 2702 Fragments arranged by size: 2702 2129 2006 711 258 136 BsaBI GATnn'nnATC Cuts at: 7936 7936 Size: 7942 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3306 4488 5043 5749 Size: 53 83 186 936 1773 1182 555 706 Cuts at: 5749 6131 6766 275 Size: 382 635 1451 Fragments arranged by size: 1773 1451 1182 936 706 635 555 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4297 6375 6773 7047 7194 7605 4297 Size: 2078 398 274 147 411 4634 Fragments arranged by size: 4634 2078 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2016 2046 2427 2457 2714 Size: 30 165 30 613 30 381 30 257 Cuts at: 2714 2744 2815 2845 3382 3412 3863 3893 4400 Size: 30 71 30 537 30 451 30 507 Cuts at: 4400 4430 1178 Size: 30 4690 Fragments arranged by size: 4690 613 537 507 451 381 257 165 71 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2300 2303 3403 3524 5203 1186 Size: 1114 3 1100 121 1679 3925 Fragments arranged by size: 3925 1679 1114 1100 121 3 BseYI C'CCAG_C Cuts at: 2056 2733 3083 4024 4885 4909 4966 5126 7145 Size: 677 350 941 861 24 57 160 2019 Cuts at: 7145 7641 2056 Size: 496 2357 Fragments arranged by size: 2357 2019 941 861 677 496 350 160 57 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3421 1163 Size: 573 1685 5684 Fragments arranged by size: 5684 1685 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5392 6153 7181 1540 Size: 275 186 3391 761 1028 2301 Fragments arranged by size: 3391 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3318 3593 4668 6003 6088 7159 3318 Size: 275 1075 1335 85 1071 4101 Fragments arranged by size: 4101 1335 1075 1071 275 85 BsiWI C'GTAC_G Cuts at: 3210 3210 Size: 7942 BsmI GAATG_Cn' Cuts at: 4757 5199 5453 5546 4757 Size: 442 254 93 7153 Fragments arranged by size: 7153 442 254 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2905 2905 Size: 7942 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2260 2828 2994 3119 3318 3593 3652 Size: 399 470 568 166 125 199 275 59 Cuts at: 3652 3791 4512 4668 4685 4716 5231 5261 6003 Size: 139 721 156 17 31 515 30 742 Cuts at: 6003 6088 7159 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 721 568 515 470 399 275 199 166 156 139 125 85 59 31 30 17 BspEI T'CCGG_A Cuts at: 4297 7605 4297 Size: 3308 4634 BspHI T'CATG_A Cuts at: 5831 7561 5831 Size: 1730 6212 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2702 2915 3231 3302 4070 4688 5297 844 Size: 1858 213 316 71 768 618 609 3489 Fragments arranged by size: 3489 1858 768 618 609 316 213 71 BsrBI CCG'CTC Cuts at: 1394 1823 4310 4522 5829 1394 Size: 429 2487 212 1307 3507 Fragments arranged by size: 3507 2487 1307 429 212 BsrDI GCAATG_nn' Cuts at: 65 1248 6437 6611 65 Size: 1183 5189 174 1396 Fragments arranged by size: 5189 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3421 6586 6773 1700 Size: 110 1611 3165 187 2869 Fragments arranged by size: 3165 2869 1611 187 110 BsrGI T'GTAC_A Cuts at: 96 2386 96 Size: 2290 5652 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7942 BssSI C'ACGA_G Cuts at: 6002 7014 6002 Size: 1012 6930 BstAPI GCAn_nnn'nTGC Cuts at: 794 3786 794 Size: 2992 4950 BstBI TT'CG_AA Cuts at: 6746 6746 Size: 7942 BstEII G'GTnAC_C Cuts at: 1184 2061 4082 1184 Size: 877 2021 5044 Fragments arranged by size: 5044 2021 877 BstXI CCAn_nnnn'nTGG Cuts at: 2351 2624 3616 3848 5143 2351 Size: 273 992 232 1295 5150 Fragments arranged by size: 5150 1295 992 273 232 BstYI r'GATC_y Cuts at: 1972 2324 2715 3522 4413 5163 5272 5639 6024 Size: 352 391 807 891 750 109 367 385 Cuts at: 6024 6041 7493 7579 7591 7937 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 891 807 750 391 385 367 352 346 109 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2810 3300 4739 2810 Size: 490 1439 6013 Fragments arranged by size: 6013 1439 490 BtgI C'CryG_G Cuts at: 513 1065 2004 2878 3105 4839 4998 5204 513 Size: 552 939 874 227 1734 159 206 3251 Fragments arranged by size: 3251 1734 939 874 552 227 206 159 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7203 Fragments arranged by size: 7203 683 56 BtsI GCAGTG_nn' Cuts at: 2024 3899 4233 4260 5458 6252 6272 2024 Size: 1875 334 27 1198 794 20 3694 Fragments arranged by size: 3694 1875 1198 794 334 27 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7942 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2603 2638 4674 4709 529 Size: 35 2039 35 2036 35 3762 Fragments arranged by size: 3762 2039 2036 35 35 35 DraI TTT'AAA Cuts at: 5246 5246 Size: 7942 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2860 6949 809 Size: 507 1544 4089 1802 Fragments arranged by size: 4089 1802 1544 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2227 2881 Size: 54 1425 50 275 18 207 190 654 Cuts at: 2881 3340 3606 4218 4464 4563 4806 4842 4974 Size: 459 266 612 246 99 243 36 132 Cuts at: 4974 5389 6278 7907 8 Size: 415 889 1629 43 Fragments arranged by size: 1629 1425 889 654 612 459 415 275 266 246 243 207 190 132 99 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 5389 1537 Size: 275 3577 4090 Fragments arranged by size: 4090 3577 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 2470 2484 4175 5872 1353 Size: 699 418 14 1691 1697 3423 Fragments arranged by size: 3423 1697 1691 699 418 14 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4415 6529 6903 7049 387 Size: 1064 138 2826 2114 374 146 1280 Fragments arranged by size: 2826 2114 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2331 2413 2623 3133 3141 3674 3796 Size: 393 699 82 210 510 8 533 122 Cuts at: 3796 4078 4488 4871 5021 5144 6005 6602 7389 Size: 282 410 383 150 123 861 597 787 Cuts at: 7389 7657 1239 Size: 268 1524 Fragments arranged by size: 1524 861 787 699 597 533 510 410 393 383 282 268 210 150 123 122 82 8 EcoNI CCTnn'n_nnAGG Cuts at: 2282 2975 4742 2282 Size: 693 1767 5482 Fragments arranged by size: 5482 1767 693 EcoO109I rG'GnC_Cy Cuts at: 2141 2682 3253 3355 3682 4508 4509 4591 4880 Size: 541 571 102 327 826 1 82 289 Cuts at: 4880 2141 Size: 5203 Fragments arranged by size: 5203 826 571 541 327 289 102 82 1 EcoRI G'AATT_C Cuts at: 5251 5629 5251 Size: 378 7564 FspI TGC'GCA Cuts at: 5099 6448 5099 Size: 1349 6593 HaeII r_GCGC'y Cuts at: 1678 2372 3190 4319 4358 4491 4900 7089 1678 Size: 694 818 1129 39 133 409 2189 2531 Fragments arranged by size: 2531 2189 1129 818 694 409 133 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2425 2457 2758 2790 2815 Size: 32 135 32 853 32 301 32 25 Cuts at: 2815 2847 3686 3718 4095 4127 6583 6615 6657 Size: 32 839 32 377 32 2456 32 42 Cuts at: 6657 6689 1373 Size: 32 2626 Fragments arranged by size: 2626 2456 853 839 377 301 135 42 32 32 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 2518 5286 5532 669 Size: 988 281 580 2768 246 3079 Fragments arranged by size: 3079 2768 988 580 281 246 HindIII A'AGCT_T Cuts at: 748 3135 5091 748 Size: 2387 1956 3599 Fragments arranged by size: 3599 2387 1956 HpaI GTT'AAC Cuts at: 5532 5532 Size: 7942 KasI G'GCGC_C Cuts at: 4487 4487 Size: 7942 KpnI G_GTAC'C Cuts at: 5267 5267 Size: 7942 MfeI C'AATT_G Cuts at: 5541 5541 Size: 7942 MluI A'CGCG_T Cuts at: 7818 7818 Size: 7942 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2928 3594 4740 7056 7240 1305 Size: 1623 666 1146 2316 184 2007 Fragments arranged by size: 2316 2007 1623 1146 666 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2039 3342 3608 4220 4565 Size: 54 1425 343 207 1303 266 612 345 Cuts at: 4565 4808 4844 4976 10 Size: 243 36 132 2976 Fragments arranged by size: 2976 1425 1303 612 345 343 266 243 207 132 54 36 MslI CAynn'nnrTG Cuts at: 518 1166 4790 4817 4844 5900 6259 6418 518 Size: 648 3624 27 27 1056 359 159 2042 Fragments arranged by size: 3624 2042 1056 648 359 159 27 27 MspA1I CmG'CkG Cuts at: 1606 2737 3031 3067 3107 3435 3535 3844 4612 Size: 1131 294 36 40 328 100 309 768 Cuts at: 4612 4633 5172 5358 6035 7183 7428 1606 Size: 21 539 186 677 1148 245 2120 Fragments arranged by size: 2120 1148 1131 768 677 539 328 309 294 245 186 100 40 36 21 NarI GG'CG_CC Cuts at: 4488 4488 Size: 7942 NcoI C'CATG_G Cuts at: 513 1065 2004 4839 5204 513 Size: 552 939 2835 365 3251 Fragments arranged by size: 3251 2835 939 552 365 NdeI CA'TA_TG Cuts at: 387 387 Size: 7942 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7942 NotI GC'GGCC_GC Cuts at: 5389 5389 Size: 7942 NspI r_CATG'y Cuts at: 1132 1229 2911 3780 5191 5300 6845 1132 Size: 97 1682 869 1411 109 1545 2229 Fragments arranged by size: 2229 1682 1545 1411 869 109 97 PasI CC'CwG_GG Cuts at: 1348 2112 2819 2953 3735 4708 1348 Size: 764 707 134 782 973 4582 Fragments arranged by size: 4582 973 782 764 707 134 PciI A'CATG_T Cuts at: 2907 6841 2907 Size: 3934 4008 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7942 PfoI T'CCnGG_A Cuts at: 2337 2429 3276 2337 Size: 92 847 7003 Fragments arranged by size: 7003 847 92 PmeI GTTT'AAAC Cuts at: 5246 5246 Size: 7942 PmlI CAC'GTG Cuts at: 3346 3346 Size: 7942 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4400 4432 6011 6043 7524 7556 4400 Size: 32 1579 32 1481 32 4786 Fragments arranged by size: 4786 1579 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2141 2682 3253 3682 4880 2141 Size: 541 571 429 1198 5203 Fragments arranged by size: 5203 1198 571 541 429 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7942 PsiI TTA'TAA Cuts at: 5512 5512 Size: 7942 PspOMI G'GGCC_C Cuts at: 2256 4508 2256 Size: 2252 5690 PstI C_TGCA'G Cuts at: 830 2127 2707 2742 2847 3228 3486 3837 3906 Size: 1297 580 35 105 381 258 351 69 Cuts at: 3906 5294 830 Size: 1388 3478 Fragments arranged by size: 3478 1388 1297 580 381 351 258 105 69 35 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7942 PvuII CAG'CTG Cuts at: 2737 3031 3067 3435 3535 3844 4612 4633 5172 Size: 294 36 368 100 309 768 21 539 Cuts at: 5172 2737 Size: 5507 Fragments arranged by size: 5507 768 539 368 309 294 100 36 21 SacII CC_GC'GG Cuts at: 3108 3108 Size: 7942 SalI G'TCGA_C Cuts at: 1936 5284 1936 Size: 3348 4594 SanDI GG'GwC_CC Cuts at: 2141 2682 3253 4880 2141 Size: 541 571 1627 5203 Fragments arranged by size: 5203 1627 571 541 SbfI CC_TGCA'GG Cuts at: 5294 5294 Size: 7942 ScaI AGT'ACT Cuts at: 6190 7902 6190 Size: 1712 6230 SexAI A'CCwGG_T Cuts at: 3698 3698 Size: 7942 SfcI C'TryA_G Cuts at: 826 1045 2123 2703 2738 2843 3224 3482 3833 Size: 219 1078 580 35 105 381 258 351 Cuts at: 3833 3871 3902 5290 6425 7106 7297 826 Size: 38 31 1388 1135 681 191 1471 Fragments arranged by size: 1471 1388 1135 1078 681 580 381 351 258 219 191 105 38 35 31 SfoI GGC'GCC Cuts at: 4489 4489 Size: 7942 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7942 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7942 SmaI CCC'GGG Cuts at: 1872 2656 2713 5269 1872 Size: 784 57 2556 4545 Fragments arranged by size: 4545 2556 784 57 SmlI C'TyrA_G Cuts at: 820 1017 1943 4326 4725 6046 6947 7209 820 Size: 197 926 2383 399 1321 901 262 1553 Fragments arranged by size: 2383 1553 1321 926 901 399 262 197 SnaBI TAC'GTA Cuts at: 493 5733 493 Size: 5240 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7942 SphI G_CATG'C Cuts at: 1132 3780 5300 1132 Size: 2648 1520 3774 Fragments arranged by size: 3774 2648 1520 SspI AAT'ATT Cuts at: 5 52 5866 5 Size: 47 5814 2081 Fragments arranged by size: 5814 2081 47 StuI AGG'CCT Cuts at: 1517 2476 1517 Size: 959 6983 StyI C'CwwG_G Cuts at: 513 1065 2004 2238 2471 3333 3609 4839 5204 Size: 552 939 234 233 862 276 1230 365 Cuts at: 5204 5381 513 Size: 177 3074 Fragments arranged by size: 3074 1230 939 862 552 365 276 234 233 177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2832 4361 4957 5969 5986 6139 6324 Size: 1255 1252 1529 596 1012 17 153 185 Cuts at: 6324 6769 325 Size: 445 1498 Fragments arranged by size: 1529 1498 1255 1252 1012 596 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2386 2931 4377 Size: 274 80 33 51 1443 409 545 1446 Cuts at: 4377 6188 7900 96 Size: 1811 1712 138 Fragments arranged by size: 1811 1712 1446 1443 545 409 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2016 2032 2048 2064 2427 2459 3237 3269 3382 Size: 16 16 16 363 32 778 32 113 Cuts at: 3382 3414 7902 7934 2016 Size: 32 4488 32 2024 Fragments arranged by size: 4488 2024 778 363 113 32 32 32 32 16 16 16 Tth111I GACn'n_nGTC Cuts at: 1316 2301 3410 3680 1316 Size: 985 1109 270 5578 Fragments arranged by size: 5578 1109 985 270 XbaI T'CTAG_A Cuts at: 5278 5278 Size: 7942 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2409 2621 3091 3601 3925 4051 5140 2409 Size: 212 470 510 324 126 1089 5211 Fragments arranged by size: 5211 1089 510 470 324 212 126 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7942 XmaI C'CCGG_G Cuts at: 1870 2654 2711 5267 1870 Size: 784 57 2556 4545 Fragments arranged by size: 4545 2556 784 57 XmnI GAAnn'nnTTC Cuts at: 5199 6071 5199 Size: 872 7070 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5750 276 Size: 53 83 186 936 4216 2468 Fragments arranged by size: 4216 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SapI SfiI SrfI SwaI