(Circular) (Six-base) MAPSORT of: pFN21AE0359.seq Check: 556 from: 1 to: 6451 pFN21AE0359 6451 bp With 182 enzymes: SgfI * April 15, 2010 09:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4261 278 Size: 53 83 186 936 2725 2468 Fragments arranged by size: 2725 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3794 1937 Size: 1857 4594 Acc65I G'GTAC_C Cuts at: 2112 3772 2112 Size: 1660 4791 AclI AA'CG_TT Cuts at: 1563 4578 4951 1563 Size: 3015 373 3063 Fragments arranged by size: 3063 3015 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4514 5898 6166 1632 Size: 2882 1384 268 1917 Fragments arranged by size: 2882 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6254 AflIII A'CryG_T Cuts at: 1203 2538 5350 6327 1203 Size: 1335 2812 977 1327 Fragments arranged by size: 2812 1335 1327 977 AgeI A'CCGG_T Cuts at: 5282 5282 Size: 6451 AhdI GACnn_n'nnGTC Cuts at: 5180 5180 Size: 6451 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6451 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6451 AlwNI CAG_nnn'CTG Cuts at: 2645 3557 4194 5766 2645 Size: 912 637 1572 3330 Fragments arranged by size: 3330 1572 912 637 ApaLI G'TGCA_C Cuts at: 4508 5664 4508 Size: 1156 5295 ApoI r'AATT_y Cuts at: 1493 2510 3394 3501 3545 3760 3986 4138 6247 Size: 1017 884 107 44 215 226 152 2109 Cuts at: 6247 1493 Size: 1697 Fragments arranged by size: 2109 1697 1017 884 226 215 152 107 44 AseI AT'TA_AT Cuts at: 160 1032 5005 160 Size: 872 3973 1606 Fragments arranged by size: 3973 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6451 AvaI C'yCGr_G Cuts at: 1870 1943 3776 1870 Size: 73 1833 4545 Fragments arranged by size: 4545 1833 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2768 2801 2768 Size: 33 6418 BamHI G'GATC_C Cuts at: 3781 4148 3781 Size: 367 6084 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2064 2096 2112 2128 3772 Size: 325 217 627 277 32 16 16 1644 Cuts at: 3772 5227 618 Size: 1455 1842 Fragments arranged by size: 1842 1644 1455 627 325 277 217 32 16 16 BanII G_rGCy'C Cuts at: 1391 2261 2286 3770 1391 Size: 870 25 1484 4072 Fragments arranged by size: 4072 1484 870 25 BbeI G_GCGC'C Cuts at: 2132 2132 Size: 6451 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3468 928 Size: 971 1569 3911 Fragments arranged by size: 3911 1569 971 BbvCI CC'TCA_GC Cuts at: 2192 2192 Size: 6451 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4642 4676 1903 Size: 34 50 34 2621 34 3678 Fragments arranged by size: 3678 2621 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4343 5553 4343 Size: 1210 5241 BclI T'GATC_A Cuts at: 1548 3595 1548 Size: 2047 4404 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2126 2223 5062 Size: 107 122 71 631 605 454 97 2839 Cuts at: 5062 136 Size: 1525 Fragments arranged by size: 2839 1525 631 605 454 122 107 97 71 BglII A'GATC_T Cuts at: 6446 6446 Size: 6451 BlpI GC'TnA_GC Cuts at: 3740 3868 3740 Size: 128 6323 Bme1580I G_kGCm'C Cuts at: 1790 4512 5668 1790 Size: 2722 1156 2573 Fragments arranged by size: 2722 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6451 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3110 4182 5140 Size: 416 53 83 399 24 1693 1072 958 Cuts at: 5140 442 Size: 1753 Fragments arranged by size: 1753 1693 1072 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6451 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2667 2718 3339 5111 1239 Size: 1428 51 621 1772 2579 Fragments arranged by size: 2579 1772 1428 621 51 Bpu10I CC'TnA_GC Cuts at: 2192 2192 Size: 6451 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2941 4576 5441 5739 2941 Size: 1635 865 298 3653 Fragments arranged by size: 3653 1635 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5114 882 Size: 617 3615 2219 Fragments arranged by size: 3615 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4242 493 Size: 711 3038 2702 Fragments arranged by size: 3038 2702 711 BsaBI GATnn'nnATC Cuts at: 2820 6445 2820 Size: 3625 2826 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2129 4258 4640 5275 Size: 53 83 186 936 596 2129 382 635 Cuts at: 5275 275 Size: 1451 Fragments arranged by size: 2129 1451 936 635 596 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4884 5282 5556 5703 6114 4884 Size: 398 274 147 411 5221 Fragments arranged by size: 5221 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6226 Fragments arranged by size: 6226 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2127 2132 2266 2269 3201 1186 Size: 856 85 5 134 3 932 4436 Fragments arranged by size: 4436 932 856 134 85 5 3 BseYI C'CCAG_C Cuts at: 5654 6150 5654 Size: 496 5955 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3096 1163 Size: 573 1360 4518 Fragments arranged by size: 4518 1360 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2213 3901 4662 5690 1540 Size: 275 186 212 1688 761 1028 2301 Fragments arranged by size: 2301 1688 1028 761 275 212 186 BsiHKAI G_wGCw'C Cuts at: 4512 4597 5668 4512 Size: 85 1071 5295 Fragments arranged by size: 5295 1071 85 BsmI GAATG_Cn' Cuts at: 3962 4055 3962 Size: 93 6358 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2261 2286 3770 4512 4597 5668 1391 Size: 399 471 25 1484 742 85 1071 2174 Fragments arranged by size: 2174 1484 1071 742 471 399 85 25 BspEI T'CCGG_A Cuts at: 6114 6114 Size: 6451 BspHI T'CATG_A Cuts at: 3457 4340 6070 3457 Size: 883 1730 3838 Fragments arranged by size: 3838 1730 883 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3806 844 Size: 2962 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4338 1394 Size: 429 2515 3507 Fragments arranged by size: 3507 2515 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4946 5120 65 Size: 1183 3698 174 1396 Fragments arranged by size: 3698 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2149 5095 5282 1700 Size: 110 339 2946 187 2869 Fragments arranged by size: 2946 2869 339 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6451 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6451 BssSI C'ACGA_G Cuts at: 4511 5523 4511 Size: 1012 5439 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6451 BstBI TT'CG_AA Cuts at: 5255 5255 Size: 6451 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6451 BstXI CCAn_nnnn'nTGG Cuts at: 2891 2891 Size: 6451 BstYI r'GATC_y Cuts at: 1972 3487 3781 4148 4533 4550 6002 6088 6100 Size: 1515 294 367 385 17 1452 86 12 Cuts at: 6100 6446 1972 Size: 346 1977 Fragments arranged by size: 1977 1515 1452 385 367 346 294 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2030 2030 Size: 6451 BtgI C'CryG_G Cuts at: 513 1065 2165 2218 2286 513 Size: 552 1100 53 68 4678 Fragments arranged by size: 4678 1100 552 68 53 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5712 Fragments arranged by size: 5712 683 56 BtsI GCAGTG_nn' Cuts at: 2466 3967 4761 4781 2466 Size: 1501 794 20 4136 Fragments arranged by size: 4136 1501 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6451 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6416 DraI TTT'AAA Cuts at: 3755 3755 Size: 6451 DraIII CAC_nnn'GTG Cuts at: 3422 3422 Size: 6451 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5458 809 Size: 507 4142 1802 Fragments arranged by size: 4142 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2210 2274 3898 Size: 54 1425 50 275 18 380 64 1624 Cuts at: 3898 4787 6416 8 Size: 889 1629 43 Fragments arranged by size: 1629 1624 1425 889 380 275 64 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2210 3898 1537 Size: 275 398 1688 4090 Fragments arranged by size: 4090 1688 398 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4381 1353 Size: 3028 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5038 5412 5558 387 Size: 1064 138 3449 374 146 1280 Fragments arranged by size: 3449 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2667 2718 3339 4514 5111 5898 6166 Size: 393 1035 51 621 1175 597 787 268 Cuts at: 6166 1239 Size: 1524 Fragments arranged by size: 1524 1175 1035 787 621 597 393 268 51 EcoNI CCTnn'n_nnAGG Cuts at: 2910 2910 Size: 6451 EcoO109I rG'GnC_Cy Cuts at: 2454 2454 Size: 6451 EcoRI G'AATT_C Cuts at: 3545 3760 4138 3545 Size: 215 378 5858 Fragments arranged by size: 5858 378 215 FspI TGC'GCA Cuts at: 3401 4957 3401 Size: 1556 4895 HaeII r_GCGC'y Cuts at: 1678 2132 5598 1678 Size: 454 3466 2531 Fragments arranged by size: 3466 2531 454 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2473 2505 2525 2557 2978 Size: 32 135 32 901 32 20 32 421 Cuts at: 2978 3010 3071 3103 5092 5124 5166 5198 1373 Size: 32 61 32 1989 32 42 32 2626 Fragments arranged by size: 2626 1989 901 421 135 61 42 32 32 32 32 32 32 32 32 20 HincII GTy'rAC Cuts at: 669 1657 1938 3795 4041 669 Size: 988 281 1857 246 3079 Fragments arranged by size: 3079 1857 988 281 246 HindIII A'AGCT_T Cuts at: 748 2844 748 Size: 2096 4355 HpaI GTT'AAC Cuts at: 4041 4041 Size: 6451 KasI G'GCGC_C Cuts at: 2128 2128 Size: 6451 KpnI G_GTAC'C Cuts at: 2116 3776 2116 Size: 1660 4791 MfeI C'AATT_G Cuts at: 4050 4050 Size: 6451 MluI A'CGCG_T Cuts at: 6327 6327 Size: 6451 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2557 3203 5565 5749 1305 Size: 1252 646 2362 184 2007 Fragments arranged by size: 2362 2007 1252 646 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4629 Fragments arranged by size: 4629 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2496 2826 2829 2889 4409 4768 4927 Size: 648 1330 330 3 60 1520 359 159 Cuts at: 4927 518 Size: 2042 Fragments arranged by size: 2042 1520 1330 648 359 330 159 60 3 MspA1I CmG'CkG Cuts at: 1606 2070 2167 2220 3867 4544 5692 5937 1606 Size: 464 97 53 1647 677 1148 245 2120 Fragments arranged by size: 2120 1647 1148 677 464 245 97 53 NarI GG'CG_CC Cuts at: 2129 2129 Size: 6451 NcoI C'CATG_G Cuts at: 513 1065 2286 513 Size: 552 1221 4678 Fragments arranged by size: 4678 1221 552 NdeI CA'TA_TG Cuts at: 387 2998 3229 387 Size: 2611 231 3609 Fragments arranged by size: 3609 2611 231 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6451 NotI GC'GGCC_GC Cuts at: 3898 3898 Size: 6451 NruI TCG'CGA Cuts at: 2057 2057 Size: 6451 NspI r_CATG'y Cuts at: 1132 1229 2542 3406 3809 5354 1132 Size: 97 1313 864 403 1545 2229 Fragments arranged by size: 2229 1545 1313 864 403 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6451 PciI A'CATG_T Cuts at: 2538 5350 2538 Size: 2812 3639 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6451 PmeI GTTT'AAAC Cuts at: 3755 3755 Size: 6451 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4520 4552 6033 6065 4520 Size: 32 1481 32 4906 Fragments arranged by size: 4906 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6451 PsiI TTA'TAA Cuts at: 4021 4021 Size: 6451 PstI C_TGCA'G Cuts at: 830 3241 3803 830 Size: 2411 562 3478 Fragments arranged by size: 3478 2411 562 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6451 SacII CC_GC'GG Cuts at: 2168 2221 2168 Size: 53 6398 SalI G'TCGA_C Cuts at: 1936 3793 1936 Size: 1857 4594 SbfI CC_TGCA'GG Cuts at: 3803 3803 Size: 6451 ScaI AGT'ACT Cuts at: 4699 6411 4699 Size: 1712 4739 SexAI A'CCwGG_T Cuts at: 3418 3418 Size: 6451 SfcI C'TryA_G Cuts at: 826 1045 3237 3799 4934 5615 5806 826 Size: 219 2192 562 1135 681 191 1471 Fragments arranged by size: 2192 1471 1135 681 562 219 191 SfoI GGC'GCC Cuts at: 2130 2130 Size: 6451 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6451 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6451 SmaI CCC'GGG Cuts at: 1872 3778 1872 Size: 1906 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2920 4555 5456 5718 820 Size: 197 926 977 1635 901 262 1553 Fragments arranged by size: 1635 1553 977 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4242 493 Size: 3749 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6451 SphI G_CATG'C Cuts at: 1132 3809 1132 Size: 2677 3774 SspI AAT'ATT Cuts at: 5 52 4375 5 Size: 47 4323 2081 Fragments arranged by size: 4323 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6451 StyI C'CwwG_G Cuts at: 513 1065 2286 3687 3890 513 Size: 552 1221 1401 203 3074 Fragments arranged by size: 3074 1401 1221 552 203 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4478 4495 4648 4833 5278 325 Size: 1255 2898 17 153 185 445 1498 Fragments arranged by size: 2898 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4697 6409 96 Size: 274 80 33 51 1443 2720 1712 138 Fragments arranged by size: 2720 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3408 3440 3520 3552 6411 6443 3408 Size: 32 80 32 2859 32 3416 Fragments arranged by size: 3416 2859 80 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2367 1316 Size: 1051 5400 XbaI T'CTAG_A Cuts at: 3787 3787 Size: 6451 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6451 XmaI C'CCGG_G Cuts at: 1870 3776 1870 Size: 1906 4545 XmnI GAAnn'nnTTC Cuts at: 2901 4580 2901 Size: 1679 4772 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4259 276 Size: 53 83 186 936 2725 2468 Fragments arranged by size: 2725 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SfiI SrfI SwaI XcmI