Restriction map for
pFN21AB6563
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI BspDI BspEI BsrDI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MfeI
MluI MluNI MroI MscI MseI
MunI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SalI SbfI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspI StuI SwaI Tru9I Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 622 GACGTC GACGT^C
AccI 244 GTCTAC GT^[AC][GT]AC
AccII 118 CGCG CG^CG
AcsI 457 GAATTC [GA]^AATT[CT]
Aor51HI 386 AGCGCT AGC^GCT
ApoI 457 GAATTC [GA]^AATT[CT]
Asp700I 252 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 199 GGCGCC GGCGC^C
BbsI 1063 GTCTTC GAAGAC(2/6)
BbvCI 913 GCTGAGG CCTCAGC(-5/-2)
BfaI 338 CTAG C^TAG
BglI 977 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1063 GTCTTC GAAGAC(2/6)
BsaBI 183 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 595 GTGCAG GTGCAG(16/14)
BspHI 347 TCATGA T^CATGA
BssSI 948 CACGAG CACGAG(-5/-1)
BstUI 118 CGCG CG^CG
EaeI 398 CGGCCA [CT]^GGCC[GA]
Eco47III 386 AGCGCT AGC^GCT
EcoNI 39 CCTGACGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 457 GAATTC G^AATTC
EcoT14I 287 CCATGG C^C[AT][AT]GG
HphI 509 TCACC GGTGA(8/7)
KasI 199 GGCGCC G^GCGCC
KspI 117 CCGCGG CCGC^GG
MaeI 338 CTAG C^TAG
MaeIII 370 GTGAC ^GT[ACGT]AC
MamI 183 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 117 CCGCGG C[AC]G^C[GT]G
MvnI 118 CGCG CG^CG
NarI 199 GGCGCC GG^CGCC
NcoI 287 CCATGG C^CATGG
NheI 337 GCTAGC G^CTAGC
PleI 1081 GACTC GAGTC(4/5)
PpuMI 356 AGGACCC [GA]G^G[AT]CC[CT]
PstI 298 CTGCAG CTGCA^G
RcaI 347 TCATGA T^CATGA
SacII 117 CCGCGG CCGC^GG
SapI 162 GAAGAGC GCTCTTC(1/4)
ScaI 581 AGTACT AGT^ACT
SfcI 298 CTGCAG C^T[GA][CT]AG
SfoI 199 GGCGCC GGC^GCC
SmaI 65 CCCGGG CCC^GGG
SphI 557 GCATGC GCATG^C
StyI 287 CCATGG C^C[AT][AT]GG
Tsp45I 370 GTGAC ^GT[GC]AC
Tsp509I 458 AATT ^AATT
XcmI 860 CCACCTCCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 65 CCCGGG C^CCGGG
XmaI 65 CCCGGG C^CCGGG
XmnI 252 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 941 ACATGT A^C[GA][CT]GT
1030 ACATGT
AlwNI 406 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
718 CAGTGCCTG
ApaI 144 GGGCCC GGGCC^C
897 GGGCCC
BamHI 89 GGATCC G^GATCC
656 GGATCC
BanI 35 GGTGCC G^G[CT][GA]CC
199 GGCGCC
BanII 144 GGGCCC G[GA]GC[CT]^C
897 GGGCCC
Bsp1407I 944 TGTACA T^GTACA
956 TGTACA
BspLU11I 941 ACATGT A^CATGT
1030 ACATGT
BsrFI 202 GCCGGC [GA]^CCGG[CT]
424 GCCGGC
BsrGI 944 TGTACA T^GTACA
956 TGTACA
BstYI 89 GGATCC [GA]^GATC[CT]
656 GGATCC
Cfr10I 202 GCCGGC [GA]^CCGG[CT]
424 GCCGGC
DraIII 701 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
960 CACCGGGTG
EarI 1015 CTCTTC CTCTTC(1/4)
162 GAAGAG
HgaI 1010 GACGC GACGC(5/10)
447 GCGTC
Ksp632I 1015 CTCTTC CTCTTC(1/4)
162 GAAGAG
MflI 89 GGATCC [GA]^GATC[CT]
656 GGATCC
MslI 255 CATCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
948 CACGAGGATG
NaeI 202 GCCGGC GCC^GGC
424 GCCGGC
NgoMIV 202 GCCGGC G^CCGGC
424 GCCGGC
PflMI 401 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
717 CCAGTGCCTGG
SspBI 944 TGTACA T^GTACA
956 TGTACA
TfiI 303 GATTC G^A[AT]TC
472 GATTC
Van91I 401 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
717 CCAGTGCCTGG
XhoII 89 GGATCC [GA]^GATC[CT]
656 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaI AvaII BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII ItaI MaeII MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TseI TspRI TthHB8I