(Circular) (Six-base) MAPSORT of: pFN21AB6563.seq Check: 4801 from: 1 to: 5827 pFN21AB6563 5827 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2478 2478 Size: 5827 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2631 3637 278 Size: 53 83 186 936 1095 1006 2468 Fragments arranged by size: 2468 1095 1006 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2250 3170 1937 Size: 313 920 4594 Fragments arranged by size: 4594 920 313 Acc65I G'GTAC_C Cuts at: 3148 3148 Size: 5827 AclI AA'CG_TT Cuts at: 1563 3954 4327 1563 Size: 2391 373 3063 Fragments arranged by size: 3063 2391 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2153 3007 3890 5274 5542 1632 Size: 521 854 883 1384 268 1917 Fragments arranged by size: 1917 1384 883 854 521 268 AfeI AGC'GCT Cuts at: 2393 2393 Size: 5827 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5630 AflIII A'CryG_T Cuts at: 1203 2946 3035 4726 5703 1203 Size: 1743 89 1691 977 1327 Fragments arranged by size: 1743 1691 1327 977 89 AgeI A'CCGG_T Cuts at: 4658 4658 Size: 5827 AhdI GACnn_n'nnGTC Cuts at: 4556 4556 Size: 5827 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5827 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2579 1184 Size: 1395 4432 AlwNI CAG_nnn'CTG Cuts at: 2416 2728 3570 5142 2416 Size: 312 842 1572 3101 Fragments arranged by size: 3101 1572 842 312 ApaI G_GGCC'C Cuts at: 2153 2906 2153 Size: 753 5074 ApaLI G'TGCA_C Cuts at: 3884 5040 3884 Size: 1156 4671 ApoI r'AATT_y Cuts at: 1493 2462 3136 3362 3514 5623 1493 Size: 969 674 226 152 2109 1697 Fragments arranged by size: 2109 1697 969 674 226 152 AseI AT'TA_AT Cuts at: 160 1032 4381 160 Size: 872 3349 1606 Fragments arranged by size: 3349 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 AvaI C'yCGr_G Cuts at: 1870 1943 2070 2324 2984 3152 1870 Size: 73 127 254 660 168 4545 Fragments arranged by size: 4545 660 254 168 127 73 BamHI G'GATC_C Cuts at: 2094 2661 3157 3524 2094 Size: 567 496 367 4397 Fragments arranged by size: 4397 567 496 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2040 2204 3148 4603 618 Size: 325 217 627 253 164 944 1455 1842 Fragments arranged by size: 1842 1455 944 627 325 253 217 164 BanII G_rGCy'C Cuts at: 1391 2153 2906 3146 1391 Size: 762 753 240 4072 Fragments arranged by size: 4072 762 753 240 BbeI G_GCGC'C Cuts at: 2208 2208 Size: 5827 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3061 928 Size: 971 1162 3694 Fragments arranged by size: 3694 1162 971 BbvCI CC'TCA_GC Cuts at: 2919 2919 Size: 5827 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4018 4052 1903 Size: 34 2081 34 3678 Fragments arranged by size: 3678 2081 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3719 4929 3719 Size: 1210 4617 BclI T'GATC_A Cuts at: 1548 1548 Size: 5827 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2988 4438 136 Size: 107 122 71 631 605 1316 1450 1525 Fragments arranged by size: 1525 1450 1316 631 605 122 107 71 BglII A'GATC_T Cuts at: 5822 5822 Size: 5827 BlpI GC'TnA_GC Cuts at: 3244 3244 Size: 5827 Bme1580I G_kGCm'C Cuts at: 1790 2153 2906 3888 5044 1790 Size: 363 753 982 1156 2573 Fragments arranged by size: 2573 1156 982 753 363 BmgBI CAC'GTC Cuts at: 1318 2548 3079 1318 Size: 1230 531 4066 Fragments arranged by size: 4066 1230 531 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2886 3558 4516 Size: 416 53 83 399 24 1469 672 958 Cuts at: 4516 442 Size: 1753 Fragments arranged by size: 1753 1469 958 672 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2346 1056 Size: 1290 4537 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2525 2750 2870 4487 1239 Size: 1286 225 120 1617 2579 Fragments arranged by size: 2579 1617 1286 225 120 Bpu10I CC'TnA_GC Cuts at: 2541 2907 2919 2541 Size: 366 12 5449 Fragments arranged by size: 5449 366 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3952 4817 5115 3952 Size: 865 298 4664 Fragments arranged by size: 4664 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4490 882 Size: 617 2991 2219 Fragments arranged by size: 2991 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3618 493 Size: 711 2414 2702 Fragments arranged by size: 2702 2414 711 BsaBI GATnn'nnATC Cuts at: 2192 5821 2192 Size: 3629 2198 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2205 2628 3016 3634 Size: 53 83 186 936 672 423 388 618 Cuts at: 3634 4016 4651 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 672 635 618 423 388 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4260 4658 4932 5079 5490 4260 Size: 398 274 147 411 4597 Fragments arranged by size: 4597 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2579 2609 1178 Size: 30 165 30 1176 30 4396 Fragments arranged by size: 4396 1176 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2234 2237 2534 2747 2867 1186 Size: 1048 3 297 213 120 4146 Fragments arranged by size: 4146 1048 297 213 120 3 BseYI C'CCAG_C Cuts at: 5030 5526 5030 Size: 496 5331 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2621 1163 Size: 573 885 4369 Fragments arranged by size: 4369 885 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3277 4038 5066 1540 Size: 275 186 1276 761 1028 2301 Fragments arranged by size: 2301 1276 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2174 2553 3096 3888 3973 5044 2174 Size: 379 543 792 85 1071 2957 Fragments arranged by size: 2957 1071 792 543 379 85 BsmI GAATG_Cn' Cuts at: 3338 3431 3338 Size: 93 5734 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2153 2174 2553 2906 3096 3146 3888 Size: 399 363 21 379 353 190 50 742 Cuts at: 3888 3973 5044 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 379 363 353 190 85 50 21 BspEI T'CCGG_A Cuts at: 5490 5490 Size: 5827 BspHI T'CATG_A Cuts at: 2352 3716 5446 2352 Size: 1364 1730 2733 Fragments arranged by size: 2733 1730 1364 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2478 2622 3129 3182 844 Size: 1634 144 507 53 3489 Fragments arranged by size: 3489 1634 507 144 53 BsrBI CCG'CTC Cuts at: 1394 1823 2013 2163 2299 3714 1394 Size: 429 190 150 136 1415 3507 Fragments arranged by size: 3507 1415 429 190 150 136 BsrDI GCAATG_nn' Cuts at: 65 1248 4322 4496 65 Size: 1183 3074 174 1396 Fragments arranged by size: 3074 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2207 2429 4471 4658 1700 Size: 110 397 222 2042 187 2869 Fragments arranged by size: 2869 2042 397 222 187 110 BsrGI T'GTAC_A Cuts at: 96 2949 2961 96 Size: 2853 12 2962 Fragments arranged by size: 2962 2853 12 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5827 BssSI C'ACGA_G Cuts at: 2953 3887 4899 2953 Size: 934 1012 3881 Fragments arranged by size: 3881 1012 934 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5827 BstBI TT'CG_AA Cuts at: 4631 4631 Size: 5827 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5827 BstYI r'GATC_y Cuts at: 1972 2094 2661 3157 3524 3909 3926 5378 5464 Size: 122 567 496 367 385 17 1452 86 Cuts at: 5464 5476 5822 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 567 496 385 367 346 122 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2122 2200 2292 513 Size: 552 939 118 78 92 4048 Fragments arranged by size: 4048 939 552 118 92 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5088 Fragments arranged by size: 5088 683 56 BtsI GCAGTG_nn' Cuts at: 2417 3343 4137 4157 2417 Size: 926 794 20 4087 Fragments arranged by size: 4087 926 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5827 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5792 DraI TTT'AAA Cuts at: 3131 3131 Size: 5827 DraIII CAC_nnn'GTG Cuts at: 2711 2970 2711 Size: 259 5568 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4834 809 Size: 507 3518 1802 Fragments arranged by size: 3518 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2403 3274 4163 Size: 54 1425 50 275 18 573 871 889 Cuts at: 4163 5792 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 871 573 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3274 1537 Size: 275 1462 4090 Fragments arranged by size: 4090 1462 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2162 3026 3757 1353 Size: 809 864 731 3423 Fragments arranged by size: 3423 864 809 731 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4414 4788 4934 387 Size: 1064 138 2825 374 146 1280 Fragments arranged by size: 2825 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2153 2525 2750 2870 3007 3890 4487 Size: 393 521 372 225 120 137 883 597 Cuts at: 4487 5274 5542 1239 Size: 787 268 1524 Fragments arranged by size: 1524 883 787 597 521 393 372 268 225 137 120 EcoNI CCTnn'n_nnAGG Cuts at: 2048 2048 Size: 5827 EcoO109I rG'GnC_Cy Cuts at: 2362 2902 2903 2362 Size: 540 1 5286 Fragments arranged by size: 5286 540 1 EcoRI G'AATT_C Cuts at: 2462 3136 3514 2462 Size: 674 378 4775 Fragments arranged by size: 4775 674 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2191 2191 Size: 5827 FspI TGC'GCA Cuts at: 4333 4333 Size: 5827 HaeII r_GCGC'y Cuts at: 1678 2029 2208 2395 4974 1678 Size: 351 179 187 2579 2531 Fragments arranged by size: 2579 2531 351 187 179 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4468 4500 4542 4574 1373 Size: 32 135 32 2896 32 42 32 2626 Fragments arranged by size: 2896 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3171 3417 669 Size: 988 281 1233 246 3079 Fragments arranged by size: 3079 1233 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5827 HpaI GTT'AAC Cuts at: 3417 3417 Size: 5827 KasI G'GCGC_C Cuts at: 2204 2204 Size: 5827 KpnI G_GTAC'C Cuts at: 3152 3152 Size: 5827 MfeI C'AATT_G Cuts at: 3426 3426 Size: 5827 MluI A'CGCG_T Cuts at: 5703 5703 Size: 5827 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4941 5125 1305 Size: 3636 184 2007 Fragments arranged by size: 3636 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4005 Fragments arranged by size: 4005 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2264 2957 3785 4144 4303 518 Size: 648 1098 693 828 359 159 2042 Fragments arranged by size: 2042 1098 828 693 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2124 3243 3920 5068 5313 1606 Size: 518 1119 677 1148 245 2120 Fragments arranged by size: 2120 1148 1119 677 518 245 NaeI GCC'GGC Cuts at: 2209 2431 2209 Size: 222 5605 NarI GG'CG_CC Cuts at: 2205 2205 Size: 5827 NcoI C'CATG_G Cuts at: 513 1065 2004 2292 513 Size: 552 939 288 4048 Fragments arranged by size: 4048 939 552 288 NdeI CA'TA_TG Cuts at: 387 387 Size: 5827 NgoMIV G'CCGG_C Cuts at: 2207 2429 2207 Size: 222 5605 NheI G'CTAG_C Cuts at: 1052 2342 1052 Size: 1290 4537 NotI GC'GGCC_GC Cuts at: 3274 3274 Size: 5827 NspI r_CATG'y Cuts at: 1132 1229 2566 2950 3039 3185 4730 1132 Size: 97 1337 384 89 146 1545 2229 Fragments arranged by size: 2229 1545 1337 384 146 97 89 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5827 PciI A'CATG_T Cuts at: 2946 3035 4726 2946 Size: 89 1691 4047 Fragments arranged by size: 4047 1691 89 PflMI CCAn_nnn'nTGG Cuts at: 1108 2412 2728 1108 Size: 1304 316 4207 Fragments arranged by size: 4207 1304 316 PfoI T'CCnGG_A Cuts at: 2787 2847 2940 2787 Size: 60 93 5674 Fragments arranged by size: 5674 93 60 PmeI GTTT'AAAC Cuts at: 3131 3131 Size: 5827 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3896 3928 5409 5441 3896 Size: 32 1481 32 4282 Fragments arranged by size: 4282 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2362 2362 Size: 5827 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5827 PsiI TTA'TAA Cuts at: 3397 3397 Size: 5827 PspOMI G'GGCC_C Cuts at: 2149 2902 2149 Size: 753 5074 PstI C_TGCA'G Cuts at: 830 2307 3179 830 Size: 1477 872 3478 Fragments arranged by size: 3478 1477 872 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5827 SacII CC_GC'GG Cuts at: 2125 2125 Size: 5827 SalI G'TCGA_C Cuts at: 1936 3169 1936 Size: 1233 4594 SapI GCTCTTCn'nnn_ Cuts at: 2162 2162 Size: 5827 SbfI CC_TGCA'GG Cuts at: 3179 3179 Size: 5827 ScaI AGT'ACT Cuts at: 2588 4075 5787 2588 Size: 1487 1712 2628 Fragments arranged by size: 2628 1712 1487 SfcI C'TryA_G Cuts at: 826 1045 2303 3175 4310 4991 5182 826 Size: 219 1258 872 1135 681 191 1471 Fragments arranged by size: 1471 1258 1135 872 681 219 191 SfoI GGC'GCC Cuts at: 2206 2206 Size: 5827 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5827 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5827 SmaI CCC'GGG Cuts at: 1872 2072 3154 1872 Size: 200 1082 4545 Fragments arranged by size: 4545 1082 200 SmlI C'TyrA_G Cuts at: 820 1017 1943 3931 4832 5094 820 Size: 197 926 1988 901 262 1553 Fragments arranged by size: 1988 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3618 493 Size: 3125 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5827 SphI G_CATG'C Cuts at: 1132 2566 3185 1132 Size: 1434 619 3774 Fragments arranged by size: 3774 1434 619 SspI AAT'ATT Cuts at: 5 52 3751 5 Size: 47 3699 2081 Fragments arranged by size: 3699 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5827 StyI C'CwwG_G Cuts at: 513 1065 2004 2292 3266 513 Size: 552 939 288 974 3074 Fragments arranged by size: 3074 974 939 552 288 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3854 3871 4024 4209 4654 325 Size: 1255 2274 17 153 185 445 1498 Fragments arranged by size: 2274 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2487 2586 2949 Size: 274 80 33 51 1443 510 99 363 Cuts at: 2949 2961 4073 5785 96 Size: 12 1112 1712 138 Fragments arranged by size: 1712 1443 1112 510 363 274 138 99 80 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3042 3074 5787 5819 3042 Size: 32 2713 32 3050 Fragments arranged by size: 3050 2713 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5827 XbaI T'CTAG_A Cuts at: 3163 3163 Size: 5827 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2872 2872 Size: 5827 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5827 XmaI C'CCGG_G Cuts at: 1870 2070 3152 1870 Size: 200 1082 4545 Fragments arranged by size: 4545 1082 200 XmnI GAAnn'nnTTC Cuts at: 2261 3956 2261 Size: 1695 4132 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2629 3635 276 Size: 53 83 186 936 1095 1006 2468 Fragments arranged by size: 2468 1095 1006 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SrfI SwaI