Restriction map for
pFN21AE0326
(Circular) (Six-base) MAPSORT of: pFN21AE0326.seq Check: 5044 from: 1 to: 6622
pFN21AE0326 6622 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3700 3700
Size: 6622
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4432 278
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3480 3965 1937
Size: 1543 485 4594
Fragments arranged by size:
4594 1543 485
Acc65I G'GTAC_C
Cuts at: 2172 3943 2172
Size: 1771 4851
AclI AA'CG_TT
Cuts at: 1563 4749 5122 1563
Size: 3186 373 3063
Fragments arranged by size:
3186 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2184 2457 2693 3704 4685 6069 6337 1632
Size: 552 273 236 1011 981 1384 268 1917
Fragments arranged by size:
1917 1384 1011 981 552 273 268 236
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6425
AflIII A'CryG_T
Cuts at: 1203 5521 6498 1203
Size: 4318 977 1327
Fragments arranged by size:
4318 1327 977
AgeI A'CCGG_T
Cuts at: 5453 5453
Size: 6622
AhdI GACnn_n'nnGTC
Cuts at: 5351 5351
Size: 6622
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6622
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6622
AlwNI CAG_nnn'CTG
Cuts at: 2290 3637 3685 4365 5937 2290
Size: 1347 48 680 1572 2975
Fragments arranged by size:
2975 1572 1347 680 48
ApaI G_GGCC'C
Cuts at: 2803 2803
Size: 6622
ApaLI G'TGCA_C
Cuts at: 4679 5835 4679
Size: 1156 5466
ApoI r'AATT_y
Cuts at: 1493 3931 4157 4309 6418 1493
Size: 2438 226 152 2109 1697
Fragments arranged by size:
2438 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5176 160
Size: 872 4144 1606
Fragments arranged by size:
4144 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
AvaI C'yCGr_G
Cuts at: 1870 1943 2525 3243 3947 1870
Size: 73 582 718 704 4545
Fragments arranged by size:
4545 718 704 582 73
BamHI G'GATC_C
Cuts at: 3952 4319 3952
Size: 367 6255
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2172 2535 2789 3094 3943
Size: 325 217 627 385 363 254 305 849
Cuts at: 3943 5398 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 849 627 385 363 325 305
254 217
BanII G_rGCy'C
Cuts at: 1391 2521 2803 2889 3089 3552 3941 1391
Size: 1130 282 86 200 463 389 4072
Fragments arranged by size:
4072 1130 463 389 282 200 86
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2836 2963 928
Size: 971 937 127 4587
Fragments arranged by size:
4587 971 937 127
BbvCI CC'TCA_GC
Cuts at: 2217 2699 2894 2217
Size: 482 195 5945
Fragments arranged by size:
5945 482 195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4813 4847 1903
Size: 34 50 34 2792 34 3678
Fragments arranged by size:
3678 2792 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4514 5724 4514
Size: 1210 5412
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6622
BfrBI ATG'CAT
Cuts at: 3813 3813
Size: 6622
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2377 2798 2874
Size: 107 122 71 631 605 705 421 76
Cuts at: 2874 5233 136
Size: 2359 1525
Fragments arranged by size:
2359 1525 705 631 605 421 122 107
76 71
BglII A'GATC_T
Cuts at: 6617 6617
Size: 6622
BlpI GC'TnA_GC
Cuts at: 4039 4039
Size: 6622
Bme1580I G_kGCm'C
Cuts at: 1790 2500 2661 2803 3099 4683 5839 1790
Size: 710 161 142 296 1584 1156 2573
Fragments arranged by size:
2573 1584 1156 710 296 161 142
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6622
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2416 3034 3452
Size: 416 53 83 399 24 999 618 418
Cuts at: 3452 3507 3772 4353 5311 442
Size: 55 265 581 958 1753
Fragments arranged by size:
1753 999 958 618 581 418 416 399
265 83 55 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6622
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3400 3400
Size: 6622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2030 2267 2465 2495 2771 2903 3044 3162
Size: 791 237 198 30 276 132 141 118
Cuts at: 3162 5282 1239
Size: 2120 2579
Fragments arranged by size:
2579 2120 791 276 237 198 141 132
118 30
Bpu10I CC'TnA_GC
Cuts at: 2217 2699 2894 3486 2217
Size: 482 195 592 5353
Fragments arranged by size:
5353 592 482 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2702 3409 4747 5612 5910 2702
Size: 707 1338 865 298 3414
Fragments arranged by size:
3414 1338 865 707 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3323 5285 882
Size: 617 1824 1962 2219
Fragments arranged by size:
2219 1962 1824 617
BsaAI yAC'GTr
Cuts at: 493 1204 2474 4413 493
Size: 711 1270 1939 2702
Fragments arranged by size:
2702 1939 1270 711
BsaBI GATnn'nnATC
Cuts at: 6616 6616
Size: 6622
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4429 4811 5446 275
Size: 53 83 186 936 2896 382 635 1451
Fragments arranged by size:
2896 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2932 5055 5453 5727 5874 6285 2932
Size: 2123 398 274 147 411 3269
Fragments arranged by size:
3269 2123 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2463 2493 3093 3123 3430
Size: 30 165 30 1060 30 600 30 307
Cuts at: 3430 3460 1178
Size: 30 4340
Fragments arranged by size:
4340 1060 600 307 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2459 2882 1186
Size: 1273 423 4926
Fragments arranged by size:
4926 1273 423
BseYI C'CCAG_C
Cuts at: 2071 2785 2809 3337 5825 6321 2071
Size: 714 24 528 2488 496 2372
Fragments arranged by size:
2488 2372 714 528 496 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2108 3576 3712 1163
Size: 573 372 1468 136 4073
Fragments arranged by size:
4073 1468 573 372 136
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4072 4833 5861 1540
Size: 275 186 2071 761 1028 2301
Fragments arranged by size:
2301 2071 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2841 4683 4768 5839 2841
Size: 1842 85 1071 3624
Fragments arranged by size:
3624 1842 1071 85
BsmI GAATG_Cn'
Cuts at: 4133 4226 4133
Size: 93 6529
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2500 2521 2661 2803 2841 2889 3089
Size: 399 710 21 140 142 38 48 200
Cuts at: 3089 3099 3552 3941 4683 4768 5839 1391
Size: 10 453 389 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 710 453 399 389 200
142 140 85 48 38 21 10
BspEI T'CCGG_A
Cuts at: 6285 6285
Size: 6622
BspHI T'CATG_A
Cuts at: 4511 6241 4511
Size: 1730 4892
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2994 3645 3700 3861 3977 844
Size: 2150 651 55 161 116 3489
Fragments arranged by size:
3489 2150 651 161 116 55
BsrBI CCG'CTC
Cuts at: 1394 1823 4509 1394
Size: 429 2686 3507
Fragments arranged by size:
3507 2686 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3321 5117 5291 65
Size: 1183 2073 1796 174 1396
Fragments arranged by size:
2073 1796 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3621 5266 5453 1700
Size: 110 1811 1645 187 2869
Fragments arranged by size:
2869 1811 1645 187 110
BsrGI T'GTAC_A
Cuts at: 96 2635 96
Size: 2539 4083
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6622
BssSI C'ACGA_G
Cuts at: 2687 4682 5694 2687
Size: 1995 1012 3615
Fragments arranged by size:
3615 1995 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2495 794
Size: 1701 4921
BstBI TT'CG_AA
Cuts at: 5426 5426
Size: 6622
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6622
BstXI CCAn_nnnn'nTGG
Cuts at: 3140 3539 3601 3779 3140
Size: 399 62 178 5983
Fragments arranged by size:
5983 399 178 62
BstYI r'GATC_y
Cuts at: 1972 3952 4319 4704 4721 6173 6259 6271 6617
Size: 1980 367 385 17 1452 86 12 346
Cuts at: 6617 1972
Size: 1977
Fragments arranged by size:
1980 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2012 2562 3305 2012
Size: 550 743 5329
Fragments arranged by size:
5329 743 550
BtgI C'CryG_G
Cuts at: 513 1065 2298 3114 3594 513
Size: 552 1233 816 480 3541
Fragments arranged by size:
3541 1233 816 552 480
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2111 487
Size: 683 56 885 4998
Fragments arranged by size:
4998 885 683 56
BtsI GCAGTG_nn'
Cuts at: 2270 2857 3619 3890 4138 4932 4952 2270
Size: 587 762 271 248 794 20 3940
Fragments arranged by size:
3940 794 762 587 271 248 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6622
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6587
DraI TTT'AAA
Cuts at: 3926 3926
Size: 6622
DraIII CAC_nnn'GTG
Cuts at: 2495 2495
Size: 6622
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3447 5629 809
Size: 507 2131 2182 1802
Fragments arranged by size:
2182 2131 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2295 2742 3111
Size: 54 1425 50 275 18 465 447 369
Cuts at: 3111 3471 3744 4069 4958 6587 8
Size: 360 273 325 889 1629 43
Fragments arranged by size:
1629 1425 889 465 447 369 360 325
275 273 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4069 1537
Size: 275 2257 4090
Fragments arranged by size:
4090 2257 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2226 2436 4552 1353
Size: 873 210 2116 3423
Fragments arranged by size:
3423 2116 873 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5209 5583 5729 387
Size: 1064 138 3620 374 146 1280
Fragments arranged by size:
3620 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2184 2267 2457 2465 2495 2693
Size: 393 398 154 83 190 8 30 198
Cuts at: 2693 2771 2903 3044 3162 3704 4685 5282 6069
Size: 78 132 141 118 542 981 597 787
Cuts at: 6069 6337 1239
Size: 268 1524
Fragments arranged by size:
1524 981 787 597 542 398 393 268
198 190 154 141 132 118 83 78
30 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2483 3290 2483
Size: 807 5815
EcoO109I rG'GnC_Cy
Cuts at: 2251 2664 2799 2983 3055 2251
Size: 413 135 184 72 5818
Fragments arranged by size:
5818 413 184 135 72
EcoRI G'AATT_C
Cuts at: 3931 4309 3931
Size: 378 6244
FspI TGC'GCA
Cuts at: 5128 5128
Size: 6622
HaeII r_GCGC'y
Cuts at: 1678 5769 1678
Size: 4091 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2600 2632 3534 3566 5263
Size: 32 135 32 1028 32 902 32 1697
Cuts at: 5263 5295 5337 5369 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1697 1028 902 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2451 3966 4212 669
Size: 988 281 513 1515 246 3079
Fragments arranged by size:
3079 1515 988 513 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6622
HpaI GTT'AAC
Cuts at: 4212 4212
Size: 6622
KpnI G_GTAC'C
Cuts at: 2176 3947 2176
Size: 1771 4851
MfeI C'AATT_G
Cuts at: 4221 4221
Size: 6622
MluI A'CGCG_T
Cuts at: 6498 6498
Size: 6622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2982 3431 5736 5920 1305
Size: 1677 449 2305 184 2007
Fragments arranged by size:
2305 2007 1677 449 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2744 3113 3473 3746 10
Size: 54 1425 343 912 369 360 273 2886
Fragments arranged by size:
2886 1425 912 369 360 343 273 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2687 4580 4939 5098 518
Size: 648 1521 1893 359 159 2042
Fragments arranged by size:
2042 1893 1521 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2287 3430 4038 4715 5863 6108 1606
Size: 681 1143 608 677 1148 245 2120
Fragments arranged by size:
2120 1148 1143 681 677 608 245
NaeI GCC'GGC
Cuts at: 3623 3623
Size: 6622
NcoI C'CATG_G
Cuts at: 513 1065 3114 3594 513
Size: 552 2049 480 3541
Fragments arranged by size:
3541 2049 552 480
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6622
NgoMIV G'CCGG_C
Cuts at: 3621 3621
Size: 6622
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6622
NotI GC'GGCC_GC
Cuts at: 4069 4069
Size: 6622
NsiI A_TGCA'T
Cuts at: 3815 3815
Size: 6622
NspI r_CATG'y
Cuts at: 1132 1229 3047 3980 5525 1132
Size: 97 1818 933 1545 2229
Fragments arranged by size:
2229 1818 1545 933 97
PasI CC'CwG_GG
Cuts at: 1348 2661 1348
Size: 1313 5309
PciI A'CATG_T
Cuts at: 5521 5521
Size: 6622
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6622
PfoI T'CCnGG_A
Cuts at: 2481 3771 2481
Size: 1290 5332
PmeI GTTT'AAAC
Cuts at: 3926 3926
Size: 6622
PmlI CAC'GTG
Cuts at: 2474 2474
Size: 6622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4691 4723 6204 6236 4691
Size: 32 1481 32 5077
Fragments arranged by size:
5077 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2983 2983
Size: 6622
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6622
PsiI TTA'TAA
Cuts at: 4192 4192
Size: 6622
PspOMI G'GGCC_C
Cuts at: 2799 2799
Size: 6622
PstI C_TGCA'G
Cuts at: 830 2553 2991 3642 3974 830
Size: 1723 438 651 332 3478
Fragments arranged by size:
3478 1723 651 438 332
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6622
PvuII CAG'CTG
Cuts at: 2287 3430 2287
Size: 1143 5479
SalI G'TCGA_C
Cuts at: 1936 3964 1936
Size: 2028 4594
SbfI CC_TGCA'GG
Cuts at: 3974 3974
Size: 6622
ScaI AGT'ACT
Cuts at: 4870 6582 4870
Size: 1712 4910
SfcI C'TryA_G
Cuts at: 826 1045 2549 2987 3638 3970 5105 5786 5977
Size: 219 1504 438 651 332 1135 681 191
Cuts at: 5977 826
Size: 1471
Fragments arranged by size:
1504 1471 1135 681 651 438 332 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6622
SmaI CCC'GGG
Cuts at: 1872 3949 1872
Size: 2077 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2717 3388 4726 5627 5889 820
Size: 197 926 774 671 1338 901 262 1553
Fragments arranged by size:
1553 1338 926 901 774 671 262 197
SnaBI TAC'GTA
Cuts at: 493 4413 493
Size: 3920 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6622
SphI G_CATG'C
Cuts at: 1132 3980 1132
Size: 2848 3774
SspI AAT'ATT
Cuts at: 5 52 4546 5
Size: 47 4494 2081
Fragments arranged by size:
4494 2081 47
StuI AGG'CCT
Cuts at: 1517 2612 3203 3380 3741 1517
Size: 1095 591 177 361 4398
Fragments arranged by size:
4398 1095 591 361 177
StyI C'CwwG_G
Cuts at: 513 1065 2372 3114 3594 4061 513
Size: 552 1307 742 480 467 3074
Fragments arranged by size:
3074 1307 742 552 480 467
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2553 2777 2886 4649 4666 4819 5004
Size: 1255 973 224 109 1763 17 153 185
Cuts at: 5004 5449 325
Size: 445 1498
Fragments arranged by size:
1763 1498 1255 973 445 224 185 153
109 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 2635 4868
Size: 274 80 33 51 1443 258 400 2233
Cuts at: 4868 6580 96
Size: 1712 138
Fragments arranged by size:
2233 1712 1443 400 274 258 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2463 2495 3093 3125 6582 6614 2463
Size: 32 598 32 3457 32 2471
Fragments arranged by size:
3457 2471 598 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6622
XbaI T'CTAG_A
Cuts at: 3958 3958
Size: 6622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2176 2403 3800 2176
Size: 227 1397 4998
Fragments arranged by size:
4998 1397 227
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6622
XmaI C'CCGG_G
Cuts at: 1870 3947 1870
Size: 2077 4545
XmnI GAAnn'nnTTC
Cuts at: 2960 3231 4751 2960
Size: 271 1520 4831
Fragments arranged by size:
4831 1520 271
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4430 276
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NarI
NruI PacI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI