Restriction map for
pFN21AB6490
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AvaI AviII AvrII
BanI BbeI BbrPI BcgI BclI
BcnI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
CelII CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AfaI 837 GTAC GT^AC
AlwNI 1039 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 114 CCTCAGG CC^T[ACGT]AGG
Asp700I 120 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 242 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 351 TGGCCA TGG^CCA
BamHI 35 GGATCC G^GATCC
BanII 342 GAGCCC G[GA]GC[CT]^C
BbsI 961 GTCTTC GAAGAC(2/6)
BbvCI 885 CCTCAGC CCTCAGC(-5/-2)
BciVI 267 GGATAC GTATCC(6/5)
BglI 344 GCCCCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 961 GTCTTC GAAGAC(2/6)
BsaAI 382 TACGTG [CT]AC^GT[GA]
BseRI 781 CTCCTC GAGGAG(10/8)
BsmFI 684 GGGAC GGGAC(10/14)
BsmI 120 GAATGC GAATGC(1/-1)
Bsp1407I 836 TGTACA T^GTACA
BspMI 370 GCAGGT ACCTGC(4/8)
BsrDI 564 CATTGC GCAATG(2/0)
BsrGI 836 TGTACA T^GTACA
Bst1107I 376 GTATAC GTA^TAC
BstYI 35 GGATCC [GA]^GATC[CT]
BstZ17I 376 GTATAC GTA^TAC
Bsu36I 114 CCTCAGG CC^T[ACGT]AGG
DraII 1040 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 359 CCATGG C^C[GA][CT]GG
EaeI 351 TGGCCA [CT]^GGCC[GA]
EarI 136 CTCTTC CTCTTC(1/4)
Eco81I 114 CCTCAGG CC^T[ACGT]AGG
EcoNI 1032 CCTCGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1040 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 359 CCATGG C^C[AT][AT]GG
HgaI 246 GCGTC GACGC(5/10)
HincII 42 GTCAAC GT[CT]^[GA]AC
HindII 42 GTCAAC GT[CT]^[GA]AC
HphI 60 GGTGA GGTGA(8/7)
Ksp632I 136 CTCTTC CTCTTC(1/4)
MflI 35 GGATCC [GA]^GATC[CT]
MluNI 351 TGGCCA TGG^CCA
MscI 351 TGGCCA TGG^CCA
MspA1I 487 CAGCTG C[AC]G^C[GT]G
MwoI 344 GCCCCTCTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 359 CCATGG C^CATGG
NspI 833 GCATGT [GA]CATG^[CT]
PflFI 242 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1040 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 487 CAGCTG CAG^CTG
RsaI 837 GTAC GT^AC
SspBI 836 TGTACA T^GTACA
SspI 623 AATATT AAT^ATT
StyI 359 CCATGG C^C[AT][AT]GG
TaqI 953 TCGA T^CGA
Tsp509I 64 AATT ^AATT
TspRI 1014 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 242 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 953 TCGA T^CGA
XhoII 35 GGATCC [GA]^GATC[CT]
XmnI 120 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 376 GTATAC GT^[AC][GT]AC
694 GTAGAC
AlwI 36 GATCC GGATC(4/5)
35 GGATC
BsgI 916 CTGCAC GTGCAG(16/14)
484 GTGCAG
BsmAI 203 GAGAC GTCTC(1/5)
328 GAGAC
BstXI 24 CCATGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
250 CCATACTGGTGG
DpnII 36 GATC ^GATC
105 GATC
MboI 36 GATC ^GATC
105 GATC
MseI 176 TTAA T^TAA
224 TTAA
NdeII 36 GATC ^GATC
105 GATC
PflMI 354 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1038 CCAGGTCCTGG
Sau3AI 36 GATC ^GATC
105 GATC
SfaNI 599 GCATC GCATC(5/9)
1006 GCATC
SfcI 279 CTACAG C^T[GA][CT]AG
435 CTACAG
Tru9I 176 TTAA T^TAA
224 TTAA
Tsp45I 391 GTCAC ^GT[GC]AC
504 GTCAC
Van91I 354 CCATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1038 CCAGGTCCTGG
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI AvaII BbvI BmyI
BpmI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeII MaeIII
MboII MnlI MslI MvaI NlaIII
NlaIV PleI Sau96I ScrFI SmlI
TfiI TseI