(Circular) (Six-base) MAPSORT of: pFN21AB6490.seq Check: 3434 from: 1 to: 5776 pFN21AB6490 5776 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2366 2366 Size: 5776 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2382 2700 3119 1937 Size: 445 318 419 4594 Fragments arranged by size: 4594 445 419 318 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2147 3839 5223 5491 1632 Size: 515 1692 1384 268 1917 Fragments arranged by size: 1917 1692 1384 515 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 4675 5652 1203 Size: 3472 977 1327 Fragments arranged by size: 3472 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 2297 1166 Size: 1131 4645 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2007 2039 2007 Size: 32 5744 AlwNI CAG_nnn'CTG Cuts at: 3049 3519 5091 3049 Size: 470 1572 3734 Fragments arranged by size: 3734 1572 470 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 3085 3311 3463 5572 1493 Size: 1592 226 152 2109 1697 Fragments arranged by size: 2109 1697 1592 226 152 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 3101 1870 Size: 73 1158 4545 Fragments arranged by size: 4545 1158 73 BamHI G'GATC_C Cuts at: 2040 3106 3473 2040 Size: 1066 367 4343 Fragments arranged by size: 4343 1066 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3097 4552 618 Size: 325 217 627 1310 1455 1842 Fragments arranged by size: 1842 1455 1310 627 325 217 BanII G_rGCy'C Cuts at: 1391 2351 3095 1391 Size: 960 744 4072 Fragments arranged by size: 4072 960 744 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2959 928 Size: 971 1060 3745 Fragments arranged by size: 3745 1060 971 BbvCI CC'TCA_GC Cuts at: 2891 2891 Size: 5776 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2266 3668 4878 2266 Size: 1402 1210 3164 Fragments arranged by size: 3164 1402 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2355 4387 136 Size: 107 122 71 631 605 683 2032 1525 Fragments arranged by size: 2032 1525 683 631 605 122 107 71 BglII A'GATC_T Cuts at: 5771 5771 Size: 5776 BlpI GC'TnA_GC Cuts at: 3193 3193 Size: 5776 Bme1580I G_kGCm'C Cuts at: 1790 2304 2372 2804 3006 3837 4993 1790 Size: 514 68 432 202 831 1156 2573 Fragments arranged by size: 2573 1156 831 514 432 202 68 BmgBI CAC'GTC Cuts at: 1318 2926 1318 Size: 1608 4168 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2702 3507 4465 Size: 416 53 83 399 24 1285 805 958 Cuts at: 4465 442 Size: 1753 Fragments arranged by size: 1753 1285 958 805 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2231 2231 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2045 2574 2738 2818 4436 1239 Size: 806 529 164 80 1618 2579 Fragments arranged by size: 2579 1618 806 529 164 80 Bpu10I CC'TnA_GC Cuts at: 2891 2891 Size: 5776 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2606 2669 2774 3901 4766 5064 2606 Size: 63 105 1127 865 298 3318 Fragments arranged by size: 3318 1127 865 298 105 63 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4439 882 Size: 617 2940 2219 Fragments arranged by size: 2940 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2389 3567 493 Size: 711 1185 1178 2702 Fragments arranged by size: 2702 1185 1178 711 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3583 3965 4600 275 Size: 53 83 186 936 2050 382 635 1451 Fragments arranged by size: 2050 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4209 4607 4881 5028 5439 4209 Size: 398 274 147 411 4546 Fragments arranged by size: 4546 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2043 2063 2073 2093 1178 Size: 30 165 30 640 20 10 20 4861 Fragments arranged by size: 4861 640 165 30 30 20 20 10 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2777 1186 Size: 1591 4185 BseYI C'CCAG_C Cuts at: 4979 5475 4979 Size: 496 5280 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2510 2906 1163 Size: 573 774 396 4033 Fragments arranged by size: 4033 774 573 396 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3837 3922 4993 3837 Size: 85 1071 4620 Fragments arranged by size: 4620 1071 85 BsmI GAATG_Cn' Cuts at: 2131 3287 3380 2131 Size: 1156 93 4527 Fragments arranged by size: 4527 1156 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2304 2351 2372 2804 3006 3095 3837 Size: 399 514 47 21 432 202 89 742 Cuts at: 3837 3922 4993 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 514 432 399 202 89 85 47 21 BspEI T'CCGG_A Cuts at: 5439 5439 Size: 5776 BspHI T'CATG_A Cuts at: 3665 5395 3665 Size: 1730 4046 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2366 3131 844 Size: 1522 765 3489 Fragments arranged by size: 3489 1522 765 BsrBI CCG'CTC Cuts at: 1394 1823 3663 1394 Size: 429 1840 3507 Fragments arranged by size: 3507 1840 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2568 4271 4445 65 Size: 1183 1320 1703 174 1396 Fragments arranged by size: 1703 1396 1320 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4420 4607 1700 Size: 110 2610 187 2869 Fragments arranged by size: 2869 2610 187 110 BsrGI T'GTAC_A Cuts at: 96 2841 96 Size: 2745 3031 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 3836 4848 3836 Size: 1012 4764 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5776 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5776 BstXI CCAn_nnnn'nTGG Cuts at: 2036 2262 2036 Size: 226 5550 BstYI r'GATC_y Cuts at: 1972 2040 3106 3473 3858 3875 5327 5413 5425 Size: 68 1066 367 385 17 1452 86 12 Cuts at: 5425 5771 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1066 385 367 346 86 68 17 12 BstZ17I GTA'TAC Cuts at: 2383 2383 Size: 5776 Bsu36I CC'TnA_GG Cuts at: 2120 2120 Size: 5776 BtgI C'CryG_G Cuts at: 513 1065 2004 2364 513 Size: 552 939 360 3925 Fragments arranged by size: 3925 939 552 360 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 3292 4086 4106 3292 Size: 794 20 4962 Fragments arranged by size: 4962 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2758 2793 529 Size: 35 2194 35 3512 Fragments arranged by size: 3512 2194 35 35 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4783 809 Size: 507 3467 1802 Fragments arranged by size: 3467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2356 3223 4112 Size: 54 1425 50 275 18 526 867 889 Cuts at: 4112 5741 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 867 526 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2147 3706 1353 Size: 794 1559 3423 Fragments arranged by size: 3423 1559 794 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2876 4363 4737 4883 387 Size: 1064 138 1287 1487 374 146 1280 Fragments arranged by size: 1487 1287 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2147 2574 2738 2818 3839 4436 Size: 393 413 102 427 164 80 1021 597 Cuts at: 4436 5223 5491 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1021 787 597 427 413 393 268 164 102 80 EcoNI CCTnn'n_nnAGG Cuts at: 3041 3041 Size: 5776 EcoO109I rG'GnC_Cy Cuts at: 3046 3046 Size: 5776 EcoRI G'AATT_C Cuts at: 3085 3463 3085 Size: 378 5398 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 4923 1678 Size: 3245 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4417 4449 4491 4523 1373 Size: 32 135 32 2845 32 42 32 2626 Fragments arranged by size: 2845 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2049 3120 3366 669 Size: 988 281 111 1071 246 3079 Fragments arranged by size: 3079 1071 988 281 246 111 HindIII A'AGCT_T Cuts at: 748 748 Size: 5776 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4890 5074 1305 Size: 3585 184 2007 Fragments arranged by size: 3585 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2358 10 Size: 54 1425 343 526 3428 Fragments arranged by size: 3428 1425 526 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2099 2260 2297 3734 4093 4252 518 Size: 648 933 161 37 1437 359 159 2042 Fragments arranged by size: 2042 1437 933 648 359 161 159 37 MspA1I CmG'CkG Cuts at: 1606 2494 3192 3869 5017 5262 1606 Size: 888 698 677 1148 245 2120 Fragments arranged by size: 2120 1148 888 698 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2364 513 Size: 552 939 360 3925 Fragments arranged by size: 3925 939 552 360 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 2842 3134 4679 1132 Size: 97 1613 292 1545 2229 Fragments arranged by size: 2229 1613 1545 292 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 2365 3049 1108 Size: 1257 684 3835 Fragments arranged by size: 3835 1257 684 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3046 3046 Size: 5776 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PstI C_TGCA'G Cuts at: 830 3128 830 Size: 2298 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 PvuII CAG'CTG Cuts at: 2494 2494 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 4024 5736 4024 Size: 1712 4064 SfcI C'TryA_G Cuts at: 826 1045 2284 2440 3124 4259 4940 5131 826 Size: 219 1239 156 684 1135 681 191 1471 Fragments arranged by size: 1471 1239 1135 684 681 219 191 156 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 3103 1872 Size: 1231 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2621 2684 2789 3880 4781 5043 Size: 197 926 678 63 105 1091 901 262 Cuts at: 5043 820 Size: 1553 Fragments arranged by size: 1553 1091 926 901 678 262 197 105 63 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SspI AAT'ATT Cuts at: 5 52 2630 3700 5 Size: 47 2578 1070 2081 Fragments arranged by size: 2578 2081 1070 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5776 StyI C'CwwG_G Cuts at: 513 1065 2004 2364 3215 513 Size: 552 939 360 851 3074 Fragments arranged by size: 3074 939 851 552 360 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2684 3803 3820 3973 4158 4603 325 Size: 1255 1104 1119 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1119 1104 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2841 4022 5734 Size: 274 80 33 51 1443 864 1181 1712 Cuts at: 5734 96 Size: 138 Fragments arranged by size: 1712 1443 1181 864 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2043 2075 5736 5768 2043 Size: 32 3661 32 2051 Fragments arranged by size: 3661 2051 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2250 1316 Size: 934 4842 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 3101 1870 Size: 1231 4545 XmnI GAAnn'nnTTC Cuts at: 2129 3905 2129 Size: 1776 4000 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI