################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## Acc65I AccI AccIII AclI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbeI BbrPI BcgI BclI BfaI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MunI NarI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccII 360 CGCG CG^CG AcsI 948 GAATTC [GA]^AATT[CT] AflII 850 CTTAAG C^TTAAG ApoI 948 GAATTC [GA]^AATT[CT] AspHI 907 GAGCAC G[AT]GC[AT]^C AviII 1112 TGCGCA TGC^GCA BalI 1124 TGGCCA TGG^CCA BbsI 400 GTCTTC GAAGAC(2/6) BbvCI 1911 CCTCAGC CCTCAGC(-5/-2) BciVI 294 GTATCC GTATCC(6/5) BfrI 850 CTTAAG C^TTAAG BglII 887 AGATCT A^GATCT BpuAI 400 GTCTTC GAAGAC(2/6) BsiHKAI 907 GAGCAC G[AT]GC[AT]^C BsmFI 74 GGGAC GGGAC(10/14) BstAPI 1022 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 360 CGCG CG^CG DraIII 1820 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 78 CCTCATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 948 GAATTC G^AATTC FspI 1112 TGCGCA TGC^GCA HaeII 65 AGCGCC [GA]GCGC^[CT] HgaI 211 GCGTC GACGC(5/10) MluNI 1124 TGGCCA TGG^CCA MscI 1124 TGGCCA TGG^CCA MvnI 360 CGCG CG^CG NdeI 664 CATATG CA^TATG NspI 126 GCATGC [GA]CATG^[CT] PleI 1468 GACTC GAGTC(4/5) SapI 1135 GAAGAGC GCTCTTC(1/4) ScaI 698 AGTACT AGT^ACT SmaI 1797 CCCGGG CCC^GGG SphI 126 GCATGC GCATG^C TfiI 341 GATTC G^A[AT]TC XmaCI 1797 CCCGGG C^CCGGG XmaI 1797 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AatII 28 GACGTC GACGT^C 397 GACGTC AcyI 28 GACGTC G[GA]^CG[CT]C 397 GACGTC AlwI 480 GATCC GGATC(4/5) 1099 GATCC AvaI 223 CCCGAG C^[CT]CG[GA]G 1797 CCCGGG BsaHI 28 GACGTC G[GA]^CG[CT]C 397 GACGTC BsaI 415 GAGACC GGTCTC(1/5) 1568 GAGACC BsgI 1267 CTGCAC GTGCAG(16/14) 1831 CTGCAC BsoBI 223 CCCGAG C^[CT]CG[GA]G 1797 CCCGGG BspMI 1829 ACCTGC ACCTGC(4/8) 1780 GCAGGT BsrDI 207 CATTGC GCAATG(2/0) 1104 CATTGC BsrFI 130 GCCGGC [GA]^CCGG[CT] 250 GCCGGC BstEII 1683 GGTGACC G^GT[ACGT]ACC 1825 GGTGACC BstPI 1683 GGTGACC G^GT[ACGT]ACC 1825 GGTGACC Cfr10I 130 GCCGGC [GA]^CCGG[CT] 250 GCCGGC DsaI 1883 CCACGG C^C[GA][CT]GG 1931 CCGTGG EcoO65I 1683 GGTGACC G^GT[ACGT]ACC 1825 GGTGACC EcoT14I 69 CCAAGG C^C[AT][AT]GG 1572 CCTTGG Hin1I 28 GACGTC G[GA]^CG[CT]C 397 GACGTC HinfI 341 GATTC G^A[ACGT]TC 1468 GACTC MseI 446 TTAA T^TAA 851 TTAA MslI 837 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1104 CATTGCTGTG NaeI 130 GCCGGC GCC^GGC 250 GCCGGC NgoMIV 130 GCCGGC G^CCGGC 250 GCCGGC PpuMI 74 GGGACCT [GA]G^G[AT]CC[CT] 1319 AGGACCT PstI 1315 CTGCAG CTGCA^G 1892 CTGCAG PvuII 138 CAGCTG CAG^CTG 1247 CAGCTG SbfI 1314 CCTGCAGG CCTGCA^GG 1891 CCTGCAGG Sse8387I 1314 CCTGCAGG CCTGCA^GG 1891 CCTGCAGG StyI 69 CCAAGG C^C[AT][AT]GG 1572 CCTTGG Tru9I 446 TTAA T^TAA 851 TTAA Tsp509I 895 AATT ^AATT 949 AATT XcmI 725 CCATACCCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1226 CCATCCGGCCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AciI AfaI AluI ApaI AvaII BanI BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI TaqI TseI Tsp45I TspRI TthHB8I Van91I XhoII