(Linear) (Six-base) MAPSORT of: KIBB6165.seq Check: 4012 from: 1 to: 1944 KIBB6165.orf With 182 enzymes: SgfI * December 27, 2011 16:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1771 1944 Size: 1771 173 AatII G_ACGT'C Cuts at: 0 32 401 1944 Size: 32 369 1543 Fragments arranged by size: 1543 369 32 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 120 255 1944 Size: 120 135 1689 Fragments arranged by size: 1689 135 120 AflII C'TTAA_G Cuts at: 0 850 1944 Size: 850 1094 ApaI G_GGCC'C Cuts at: 0 46 224 269 1041 1449 1754 1944 Size: 46 178 45 772 408 305 190 Fragments arranged by size: 772 408 305 190 178 46 45 ApoI r'AATT_y Cuts at: 0 948 1944 Size: 948 996 AvaI C'yCGr_G Cuts at: 0 223 1797 1944 Size: 223 1574 147 Fragments arranged by size: 1574 223 147 BanI G'GyrC_C Cuts at: 0 87 768 791 1239 1430 1456 1944 Size: 87 681 23 448 191 26 488 Fragments arranged by size: 681 488 448 191 87 26 23 BanII G_rGCy'C Cuts at: 0 46 224 269 551 742 1041 1180 1449 Size: 46 178 45 282 191 299 139 269 Cuts at: 1449 1754 1874 1944 Size: 305 120 70 Fragments arranged by size: 305 299 282 269 191 178 139 120 70 46 45 BbsI GAAGACnn'nnnn_ Cuts at: 0 393 1944 Size: 393 1551 BbvCI CC'TCA_GC Cuts at: 0 1912 1944 Size: 1912 32 BciVI GTATCCnnnnn_n' Cuts at: 0 305 1944 Size: 305 1639 BglI GCCn_nnn'nGGC Cuts at: 0 101 251 273 1206 1428 1944 Size: 101 150 22 933 222 516 Fragments arranged by size: 933 516 222 150 101 22 BglII A'GATC_T Cuts at: 0 887 1944 Size: 887 1057 Bme1580I G_kGCm'C Cuts at: 0 46 224 269 794 1041 1337 1433 1449 Size: 46 178 45 525 247 296 96 16 Cuts at: 1449 1754 1821 1944 Size: 305 67 123 Fragments arranged by size: 525 305 296 247 178 123 96 67 46 45 16 BmgBI CAC'GTC Cuts at: 0 492 1944 Size: 492 1452 BmrI ACTGGGnnnn_n' Cuts at: 0 478 607 875 1831 1944 Size: 478 129 268 956 113 Fragments arranged by size: 956 478 268 129 113 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 152 484 640 1271 1888 1944 Size: 76 76 332 156 631 617 56 Fragments arranged by size: 631 617 332 156 76 76 56 Bpu10I CC'TnA_GC Cuts at: 0 1218 1912 1944 Size: 1218 694 32 Fragments arranged by size: 1218 694 32 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 331 1456 1944 Size: 331 1125 488 Fragments arranged by size: 1125 488 331 BsaI GGTCTCn'nnnn_ Cuts at: 0 409 1562 1944 Size: 409 1153 382 Fragments arranged by size: 1153 409 382 BsaHI Gr'CG_yC Cuts at: 0 29 398 1944 Size: 29 369 1546 Fragments arranged by size: 1546 369 29 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 80 110 661 691 724 754 1327 1357 Size: 80 30 551 30 33 30 573 30 Cuts at: 1357 1944 Size: 587 Fragments arranged by size: 587 573 551 80 33 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 328 490 666 732 1087 1944 Size: 328 162 176 66 355 857 Fragments arranged by size: 857 355 328 176 162 66 BseYI C'CCAG_C Cuts at: 0 246 559 631 680 1173 1245 1281 1448 Size: 246 313 72 49 493 72 36 167 Cuts at: 1448 1495 1530 1721 1918 1944 Size: 47 35 191 197 26 Fragments arranged by size: 493 313 246 197 191 167 72 72 49 47 36 35 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1252 1816 1944 Size: 1252 564 128 Fragments arranged by size: 1252 564 128 BsiHKAI G_wGCw'C Cuts at: 0 911 1944 Size: 911 1033 Bsp1286I G_dGCh'C Cuts at: 0 46 224 269 551 742 794 911 1041 Size: 46 178 45 282 191 52 117 130 Cuts at: 1041 1180 1337 1433 1449 1754 1821 1874 1944 Size: 139 157 96 16 305 67 53 70 Fragments arranged by size: 305 282 191 178 157 139 130 117 96 70 67 53 52 46 45 16 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1771 1838 1944 Size: 1771 67 106 Fragments arranged by size: 1771 106 67 BsrDI GCAATG_nn' Cuts at: 0 206 1103 1944 Size: 206 897 841 Fragments arranged by size: 897 841 206 BsrFI r'CCGG_y Cuts at: 0 130 250 1944 Size: 130 120 1694 Fragments arranged by size: 1694 130 120 BstAPI GCAn_nnn'nTGC Cuts at: 0 1028 1944 Size: 1028 916 BstEII G'GTnAC_C Cuts at: 0 1683 1825 1944 Size: 1683 142 119 Fragments arranged by size: 1683 142 119 BstXI CCAn_nnnn'nTGG Cuts at: 0 54 571 1515 1932 1944 Size: 54 517 944 417 12 Fragments arranged by size: 944 517 417 54 12 BstYI r'GATC_y Cuts at: 0 479 887 1098 1944 Size: 479 408 211 846 Fragments arranged by size: 846 479 408 211 BtgI C'CryG_G Cuts at: 0 1883 1931 1944 Size: 1883 48 13 Fragments arranged by size: 1883 48 13 BtsI GCAGTG_nn' Cuts at: 0 184 240 1264 1734 1779 1809 1944 Size: 184 56 1024 470 45 30 135 Fragments arranged by size: 1024 470 184 135 56 45 30 DraIII CAC_nnn'GTG Cuts at: 0 1825 1944 Size: 1825 119 EaeI y'GGCC_r Cuts at: 0 1049 1124 1517 1944 Size: 1049 75 393 427 Fragments arranged by size: 1049 427 393 75 EarI CTCTTCn'nnn_ Cuts at: 0 808 1084 1130 1944 Size: 808 276 46 814 Fragments arranged by size: 814 808 276 46 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 511 1187 1944 Size: 511 676 757 Fragments arranged by size: 757 676 511 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 120 152 255 484 640 1271 1888 Size: 76 44 32 103 229 156 631 617 Cuts at: 1888 1944 Size: 56 Fragments arranged by size: 631 617 229 156 103 76 56 44 32 EcoNI CCTnn'n_nnAGG Cuts at: 0 82 1944 Size: 82 1862 EcoO109I rG'GnC_Cy Cuts at: 0 43 75 220 266 1038 1222 1291 1320 Size: 43 32 145 46 772 184 69 29 Cuts at: 1320 1751 1897 1944 Size: 431 146 47 Fragments arranged by size: 772 431 184 146 145 69 47 46 43 32 29 EcoRI G'AATT_C Cuts at: 0 948 1944 Size: 948 996 FspI TGC'GCA Cuts at: 0 1114 1944 Size: 1114 830 HaeII r_GCGC'y Cuts at: 0 69 1944 Size: 69 1875 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 988 1020 1083 1115 1944 Size: 988 32 63 32 829 Fragments arranged by size: 988 829 63 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 182 1944 Size: 182 1762 MscI TGG'CCA Cuts at: 0 1126 1944 Size: 1126 818 MslI CAynn'nnrTG Cuts at: 0 841 1108 1944 Size: 841 267 836 Fragments arranged by size: 841 836 267 MspA1I CmG'CkG Cuts at: 0 140 462 1249 1944 Size: 140 322 787 695 Fragments arranged by size: 787 695 322 140 NaeI GCC'GGC Cuts at: 0 132 252 1944 Size: 132 120 1692 Fragments arranged by size: 1692 132 120 NdeI CA'TA_TG Cuts at: 0 665 1944 Size: 665 1279 NgoMIV G'CCGG_C Cuts at: 0 130 250 1944 Size: 130 120 1694 Fragments arranged by size: 1694 130 120 NspI r_CATG'y Cuts at: 0 130 1944 Size: 130 1814 PasI CC'CwG_GG Cuts at: 0 262 334 454 1204 1747 1944 Size: 262 72 120 750 543 197 Fragments arranged by size: 750 543 262 197 120 72 PflMI CCAn_nnn'nTGG Cuts at: 0 585 712 765 1944 Size: 585 127 53 1179 Fragments arranged by size: 1179 585 127 53 PfoI T'CCnGG_A Cuts at: 0 1392 1944 Size: 1392 552 PpuMI rG'GwC_Cy Cuts at: 0 75 1320 1944 Size: 75 1245 624 Fragments arranged by size: 1245 624 75 PspOMI G'GGCC_C Cuts at: 0 42 220 265 1037 1445 1750 1944 Size: 42 178 45 772 408 305 194 Fragments arranged by size: 772 408 305 194 178 45 42 PstI C_TGCA'G Cuts at: 0 1319 1896 1944 Size: 1319 577 48 Fragments arranged by size: 1319 577 48 PvuII CAG'CTG Cuts at: 0 140 1249 1944 Size: 140 1109 695 Fragments arranged by size: 1109 695 140 SapI GCTCTTCn'nnn_ Cuts at: 0 1130 1944 Size: 1130 814 SbfI CC_TGCA'GG Cuts at: 0 1319 1896 1944 Size: 1319 577 48 Fragments arranged by size: 1319 577 48 ScaI AGT'ACT Cuts at: 0 700 1944 Size: 700 1244 SfcI C'TryA_G Cuts at: 0 825 1315 1892 1944 Size: 825 490 577 52 Fragments arranged by size: 825 577 490 52 SmaI CCC'GGG Cuts at: 0 1799 1944 Size: 1799 145 SmlI C'TyrA_G Cuts at: 0 346 850 1435 1944 Size: 346 504 585 509 Fragments arranged by size: 585 509 504 346 SphI G_CATG'C Cuts at: 0 130 1944 Size: 130 1814 StuI AGG'CCT Cuts at: 0 469 508 535 1382 1944 Size: 469 39 27 847 562 Fragments arranged by size: 847 562 469 39 27 StyI C'CwwG_G Cuts at: 0 69 1572 1944 Size: 69 1503 372 Fragments arranged by size: 1503 372 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1295 1509 1813 1944 Size: 1295 214 304 131 Fragments arranged by size: 1295 304 214 131 TatI w'GTAC_w Cuts at: 0 668 698 1944 Size: 668 30 1246 Fragments arranged by size: 1246 668 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 80 112 952 984 1327 1359 1944 Size: 80 32 840 32 343 32 585 Fragments arranged by size: 840 585 343 80 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 732 1233 1944 Size: 732 501 711 Fragments arranged by size: 732 711 501 XmaI C'CCGG_G Cuts at: 0 1797 1944 Size: 1797 147 ZraI GAC'GTC Cuts at: 0 30 399 1944 Size: 30 369 1545 Fragments arranged by size: 1545 369 30 Enzymes that do cut: AarI AatII AcuI AflII ApaI ApoI AvaI BanI BanII BbsI BbvCI BciVI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BstAPI BstEII BstXI BstYI BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NdeI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SapI SbfI ScaI SfcI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmaI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BclI BfrBI BlpI BmtI BplI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmnI