Restriction map for
pFN21AE0471
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HgaI
HindIII HpaI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcyI 991 GGCGCC G[GA]^CG[CT]C
AfaI 156 GTAC GT^AC
Asp700I 330 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 237 GAGCAC G[AT]GC[AT]^C
BalI 1017 TGGCCA TGG^CCA
BamHI 816 GGATCC G^GATCC
BanI 991 GGCGCC G^G[CT][GA]CC
BbeI 991 GGCGCC GGCGC^C
BbsI 664 GAAGAC GAAGAC(2/6)
BfaI 1253 CTAG C^TAG
BpuAI 664 GAAGAC GAAGAC(2/6)
BsaHI 991 GGCGCC G[GA]^CG[CT]C
BsiHKAI 237 GAGCAC G[AT]GC[AT]^C
BsmI 654 GAATGC GAATGC(1/-1)
BsrBI 1073 CCGCTC CCGCTC(-3/-3)
BsrFI 1155 GCCGGT [GA]^CCGG[CT]
BssHII 161 GCGCGC G^CGCGC
Cfr10I 1155 GCCGGT [GA]^CCGG[CT]
DraI 318 TTTAAA TTT^AAA
EaeI 1017 TGGCCA [CT]^GGCC[GA]
EarI 187 GAAGAG CTCTTC(1/4)
FauI 1072 CCCGC CCCGC(4/6)
HaeII 991 GGCGCC [GA]GCGC^[CT]
Hin1I 991 GGCGCC G[GA]^CG[CT]C
HincII 1129 GTTGAC GT[CT]^[GA]AC
HindII 1129 GTTGAC GT[CT]^[GA]AC
KasI 991 GGCGCC G^GCGCC
Ksp632I 187 GAAGAG CTCTTC(1/4)
MaeI 1253 CTAG C^TAG
MaeII 1121 ACGT A^CGT
MaeIII 79 GTCAC ^GT[ACGT]AC
MluNI 1017 TGGCCA TGG^CCA
MscI 1017 TGGCCA TGG^CCA
MslI 12 CATGGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 536 CCGCTG C[AC]G^C[GT]G
NarI 991 GGCGCC GG^CGCC
PflMI 644 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 156 GTAC GT^AC
SfcI 288 CTGTAG C^T[GA][CT]AG
SfiI 525 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 991 GGCGCC GGC^GCC
SmaI 965 CCCGGG CCC^GGG
SmlI 1182 CTCAAG C^T[CT][GA]AG
TaqI 545 TCGA T^CGA
Tsp45I 79 GTCAC ^GT[GC]AC
TthHB8I 545 TCGA T^CGA
Van91I 644 CCAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 965 CCCGGG C^CCGGG
XmaI 965 CCCGGG C^CCGGG
XmnI 330 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 160 CGCG CG^CG
162 CGCG
AocI 820 CCTGAGG CC^T[ACGT]AGG
955 CCTCAGG
AvaI 965 CCCGGG C^[CT]CG[GA]G
1214 CTCGGG
BsgI 39 GTGCAG GTGCAG(16/14)
472 GTGCAG
BsmFI 405 GTCCC GGGAC(10/14)
941 GTCCC
BsoBI 965 CCCGGG C^[CT]CG[GA]G
1214 CTCGGG
BstUI 160 CGCG CG^CG
162 CGCG
BstYI 500 AGATCC [GA]^GATC[CT]
816 GGATCC
Bsu36I 820 CCTGAGG CC^T[ACGT]AGG
955 CCTCAGG
Eco81I 820 CCTGAGG CC^T[ACGT]AGG
955 CCTCAGG
EcoNI 24 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
207 CCTCATTGAGG
EcoT14I 11 CCATGG C^C[AT][AT]GG
917 CCATGG
MflI 500 AGATCC [GA]^GATC[CT]
816 GGATCC
MvnI 160 CGCG CG^CG
162 CGCG
NcoI 11 CCATGG C^CATGG
917 CCATGG
PleI 1132 GACTC GAGTC(4/5)
1000 GAGTC
StyI 11 CCATGG C^C[AT][AT]GG
917 CCATGG
TfiI 231 GAATC G^A[AT]TC
1303 GAATC
XhoII 500 AGATCC [GA]^GATC[CT]
816 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AluI AlwI ApaI
ApoI AvaII BanII BbvI BcnI
BglI BmyI BpmI BsaI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EcoO109I EcoRI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MboI MboII MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PpuMI Sau3AI Sau96I ScrFI
SfaNI Tru9I TseI Tsp509I TspRI