(Circular) (Six-base) MAPSORT of: pFN21AE0471.seq Check: 9352 from: 1 to: 6022 pFN21AE0471 6022 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3832 278 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3365 1937 Size: 1428 4594 Acc65I G'GTAC_C Cuts at: 3343 3343 Size: 6022 AclI AA'CG_TT Cuts at: 1563 4149 4522 1563 Size: 2586 373 3063 Fragments arranged by size: 3063 2586 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4085 5469 5737 1632 Size: 2453 1384 268 1917 Fragments arranged by size: 2453 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5825 AflIII A'CryG_T Cuts at: 1203 4921 5898 1203 Size: 3718 977 1327 Fragments arranged by size: 3718 1327 977 AgeI A'CCGG_T Cuts at: 4853 4853 Size: 6022 AhdI GACnn_n'nnGTC Cuts at: 4751 4751 Size: 6022 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6022 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6022 AlwNI CAG_nnn'CTG Cuts at: 3765 5337 3765 Size: 1572 4450 ApaI G_GGCC'C Cuts at: 2159 2533 3116 2159 Size: 374 583 5065 Fragments arranged by size: 5065 583 374 ApaLI G'TGCA_C Cuts at: 4079 5235 4079 Size: 1156 4866 ApoI r'AATT_y Cuts at: 1493 2258 2339 2546 3331 3557 3709 5818 1493 Size: 765 81 207 785 226 152 2109 1697 Fragments arranged by size: 2109 1697 785 765 226 207 152 81 AseI AT'TA_AT Cuts at: 160 1032 4576 160 Size: 872 3544 1606 Fragments arranged by size: 3544 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 AvaI C'yCGr_G Cuts at: 1870 1943 2970 3219 3347 1870 Size: 73 1027 249 128 4545 Fragments arranged by size: 4545 1027 249 128 73 BamHI G'GATC_C Cuts at: 2821 3352 3719 2821 Size: 531 367 5124 Fragments arranged by size: 5124 531 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2996 3343 4798 618 Size: 325 217 627 1209 347 1455 1842 Fragments arranged by size: 1842 1455 1209 627 347 325 217 BanII G_rGCy'C Cuts at: 1391 2159 2533 2990 3116 3341 1391 Size: 768 374 457 126 225 4072 Fragments arranged by size: 4072 768 457 374 225 126 BbeI G_GCGC'C Cuts at: 3000 3000 Size: 6022 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2676 928 Size: 971 777 4274 Fragments arranged by size: 4274 971 777 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4213 4247 1903 Size: 34 2276 34 3678 Fragments arranged by size: 3678 2276 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3914 5124 3914 Size: 1210 4812 BclI T'GATC_A Cuts at: 1548 1548 Size: 6022 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2537 2636 2994 Size: 107 122 71 631 605 865 99 358 Cuts at: 2994 4633 136 Size: 1639 1525 Fragments arranged by size: 1639 1525 865 631 605 358 122 107 99 71 BglII A'GATC_T Cuts at: 6017 6017 Size: 6022 BlpI GC'TnA_GC Cuts at: 3439 3439 Size: 6022 Bme1580I G_kGCm'C Cuts at: 1790 2159 2533 2772 3116 4083 5239 1790 Size: 369 374 239 344 967 1156 2573 Fragments arranged by size: 2573 1156 967 374 369 344 239 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6022 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2162 2449 3753 Size: 416 53 83 399 24 745 287 1304 Cuts at: 3753 4711 442 Size: 958 1753 Fragments arranged by size: 1753 1304 958 745 416 399 287 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6022 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2302 2372 2710 2730 2737 4682 1239 Size: 815 248 70 338 20 7 1945 2579 Fragments arranged by size: 2579 1945 815 338 248 70 20 7 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3172 4147 5012 5310 3172 Size: 975 865 298 3884 Fragments arranged by size: 3884 975 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2120 2292 2636 4685 882 Size: 617 621 172 344 2049 2219 Fragments arranged by size: 2219 2049 621 617 344 172 BsaAI yAC'GTr Cuts at: 493 1204 3813 493 Size: 711 2609 2702 Fragments arranged by size: 2702 2609 711 BsaBI GATnn'nnATC Cuts at: 6016 6016 Size: 6022 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2997 3829 4211 4846 Size: 53 83 186 936 1464 832 382 635 Cuts at: 4846 275 Size: 1451 Fragments arranged by size: 1464 1451 936 832 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4455 4853 5127 5274 5685 4455 Size: 398 274 147 411 4792 Fragments arranged by size: 4792 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2027 2057 2460 2490 1178 Size: 30 165 30 624 30 403 30 4710 Fragments arranged by size: 4710 624 403 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2138 2567 2915 3279 1186 Size: 952 429 348 364 3929 Fragments arranged by size: 3929 952 429 364 348 BseYI C'CCAG_C Cuts at: 5225 5721 5225 Size: 496 5526 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2065 2498 1163 Size: 573 329 433 4687 Fragments arranged by size: 4687 573 433 329 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3472 4233 5261 1540 Size: 275 186 1471 761 1028 2301 Fragments arranged by size: 2301 1471 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2246 4083 4168 5239 2246 Size: 1837 85 1071 3029 Fragments arranged by size: 3029 1837 1071 85 BsmI GAATG_Cn' Cuts at: 2665 3533 3626 2665 Size: 868 93 5061 Fragments arranged by size: 5061 868 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2159 2246 2533 2772 2990 3116 3341 Size: 399 369 87 287 239 218 126 225 Cuts at: 3341 4083 4168 5239 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 369 287 239 225 218 126 87 85 BspEI T'CCGG_A Cuts at: 5685 5685 Size: 6022 BspHI T'CATG_A Cuts at: 3911 5641 3911 Size: 1730 4292 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3377 844 Size: 2533 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3080 3909 1394 Size: 429 1257 829 3507 Fragments arranged by size: 3507 1257 829 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4517 4691 65 Size: 1183 3269 174 1396 Fragments arranged by size: 3269 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3160 4666 4853 1700 Size: 110 1350 1506 187 2869 Fragments arranged by size: 2869 1506 1350 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6022 BssHII G'CGCG_C Cuts at: 1925 2166 1925 Size: 241 5781 BssSI C'ACGA_G Cuts at: 4082 5094 4082 Size: 1012 5010 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6022 BstBI TT'CG_AA Cuts at: 4826 4826 Size: 6022 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6022 BstXI CCAn_nnnn'nTGG Cuts at: 2017 2439 2695 3020 2017 Size: 422 256 325 5019 Fragments arranged by size: 5019 422 325 256 BstYI r'GATC_y Cuts at: 1972 2505 2821 3352 3719 4104 4121 5573 5659 Size: 533 316 531 367 385 17 1452 86 Cuts at: 5659 5671 6017 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 533 531 385 367 346 316 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2826 2961 2826 Size: 135 5887 BtgI C'CryG_G Cuts at: 513 1065 2016 2664 2922 3051 513 Size: 552 951 648 258 129 3484 Fragments arranged by size: 3484 951 648 552 258 129 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2036 487 Size: 683 56 810 4473 Fragments arranged by size: 4473 810 683 56 BtsI GCAGTG_nn' Cuts at: 2478 2772 3538 4332 4352 2478 Size: 294 766 794 20 4148 Fragments arranged by size: 4148 794 766 294 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6022 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5987 DraI TTT'AAA Cuts at: 2325 3326 2325 Size: 1001 5021 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5029 809 Size: 507 3713 1802 Fragments arranged by size: 3713 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3022 3469 4358 Size: 54 1425 50 275 18 1192 447 889 Cuts at: 4358 5987 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1192 889 447 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3469 1537 Size: 275 1657 4090 Fragments arranged by size: 4090 1657 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2187 3952 1353 Size: 834 1765 3423 Fragments arranged by size: 3423 1765 834 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2369 4609 4983 5129 387 Size: 1064 138 780 2240 374 146 1280 Fragments arranged by size: 2240 1280 1064 780 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2302 2372 2710 2730 2737 4085 Size: 393 422 248 70 338 20 7 1348 Cuts at: 4085 4682 5469 5737 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1348 787 597 422 393 338 268 248 70 20 7 EcoNI CCTnn'n_nnAGG Cuts at: 2033 2216 2033 Size: 183 5839 EcoO109I rG'GnC_Cy Cuts at: 2073 2140 2529 2945 3113 3214 2073 Size: 67 389 416 168 101 4881 Fragments arranged by size: 4881 416 389 168 101 67 EcoRI G'AATT_C Cuts at: 2258 2339 2546 3331 3709 2258 Size: 81 207 785 378 4571 Fragments arranged by size: 4571 785 378 207 81 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3124 3124 Size: 6022 FspI TGC'GCA Cuts at: 4528 4528 Size: 6022 HaeII r_GCGC'y Cuts at: 1678 3000 5169 1678 Size: 1322 2169 2531 Fragments arranged by size: 2531 2169 1322 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4663 4695 4737 4769 1373 Size: 32 135 32 3091 32 42 32 2626 Fragments arranged by size: 3091 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3136 3366 3612 669 Size: 988 281 1198 230 246 3079 Fragments arranged by size: 3079 1198 988 281 246 230 HindIII A'AGCT_T Cuts at: 748 748 Size: 6022 HpaI GTT'AAC Cuts at: 3612 3612 Size: 6022 KasI G'GCGC_C Cuts at: 2996 2996 Size: 6022 KpnI G_GTAC'C Cuts at: 3347 3347 Size: 6022 MfeI C'AATT_G Cuts at: 3621 3621 Size: 6022 MluI A'CGCG_T Cuts at: 5898 5898 Size: 6022 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2823 5136 5320 1305 Size: 1518 2313 184 2007 Fragments arranged by size: 2313 2007 1518 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3024 10 Size: 54 1425 343 1192 3008 Fragments arranged by size: 3008 1425 1192 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2021 3980 4339 4498 518 Size: 648 855 1959 359 159 2042 Fragments arranged by size: 2042 1959 855 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2543 3438 4115 5263 5508 1606 Size: 937 895 677 1148 245 2120 Fragments arranged by size: 2120 1148 937 895 677 245 NarI GG'CG_CC Cuts at: 2997 2997 Size: 6022 NcoI C'CATG_G Cuts at: 513 1065 2016 2922 513 Size: 552 951 906 3613 Fragments arranged by size: 3613 951 906 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6022 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6022 NotI GC'GGCC_GC Cuts at: 3469 3469 Size: 6022 NspI r_CATG'y Cuts at: 1132 1229 3380 4925 1132 Size: 97 2151 1545 2229 Fragments arranged by size: 2229 2151 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6022 PciI A'CATG_T Cuts at: 4921 4921 Size: 6022 PflMI CCAn_nnn'nTGG Cuts at: 1108 2655 1108 Size: 1547 4475 PfoI T'CCnGG_A Cuts at: 2343 2726 2343 Size: 383 5639 PmeI GTTT'AAAC Cuts at: 3326 3326 Size: 6022 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4091 4123 5604 5636 4091 Size: 32 1481 32 4477 Fragments arranged by size: 4477 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2073 2140 2945 2073 Size: 67 805 5150 Fragments arranged by size: 5150 805 67 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6022 PsiI TTA'TAA Cuts at: 3592 3592 Size: 6022 PspOMI G'GGCC_C Cuts at: 2155 2529 3112 2155 Size: 374 583 5065 Fragments arranged by size: 5065 583 374 PstI C_TGCA'G Cuts at: 830 3374 830 Size: 2544 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6022 SalI G'TCGA_C Cuts at: 1936 3364 1936 Size: 1428 4594 SanDI GG'GwC_CC Cuts at: 2945 2945 Size: 6022 SbfI CC_TGCA'GG Cuts at: 3374 3374 Size: 6022 ScaI AGT'ACT Cuts at: 4270 5982 4270 Size: 1712 4310 SfcI C'TryA_G Cuts at: 826 1045 2293 3370 4505 5186 5377 826 Size: 219 1248 1077 1135 681 191 1471 Fragments arranged by size: 1471 1248 1135 1077 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2537 2537 Size: 6022 SfoI GGC'GCC Cuts at: 2998 2998 Size: 6022 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6022 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6022 SmaI CCC'GGG Cuts at: 1872 2972 3349 1872 Size: 1100 377 4545 Fragments arranged by size: 4545 1100 377 SmlI C'TyrA_G Cuts at: 820 1017 1943 3187 4126 5027 5289 820 Size: 197 926 1244 939 901 262 1553 Fragments arranged by size: 1553 1244 939 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3813 493 Size: 3320 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6022 SphI G_CATG'C Cuts at: 1132 3380 1132 Size: 2248 3774 SrfI GCCC'GGGC Cuts at: 2972 2972 Size: 6022 SspI AAT'ATT Cuts at: 5 52 3946 5 Size: 47 3894 2081 Fragments arranged by size: 3894 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6022 StyI C'CwwG_G Cuts at: 513 1065 2016 2922 3461 513 Size: 552 951 906 539 3074 Fragments arranged by size: 3074 951 906 552 539 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2822 3049 4049 4066 4219 4404 4849 Size: 1255 1242 227 1000 17 153 185 445 Cuts at: 4849 325 Size: 1498 Fragments arranged by size: 1498 1255 1242 1000 445 227 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4268 5980 96 Size: 274 80 33 51 1443 2291 1712 138 Fragments arranged by size: 2291 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2027 2043 2059 2075 2460 2492 5982 6014 2027 Size: 16 16 16 385 32 3490 32 2035 Fragments arranged by size: 3490 2035 385 32 32 16 16 16 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6022 XbaI T'CTAG_A Cuts at: 3358 3358 Size: 6022 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6022 XmaI C'CCGG_G Cuts at: 1870 2970 3347 1870 Size: 1100 377 4545 Fragments arranged by size: 4545 1100 377 XmnI GAAnn'nnTTC Cuts at: 2339 4151 2339 Size: 1812 4210 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3830 276 Size: 53 83 186 936 2296 2468 Fragments arranged by size: 2468 2296 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SwaI XcmI