Restriction map for
pFN21AE0471
(Linear) (Six-base) MAPSORT of: KIEE0471.seq Check: 107 from: 1 to: 1317
KIEE0471.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
ApaI G_GGCC'C
Cuts at: 0 154 528 1111 1317
Size: 154 374 583 206
Fragments arranged by size:
583 374 206 154
ApoI r'AATT_y
Cuts at: 0 253 334 541 1317
Size: 253 81 207 776
Fragments arranged by size:
776 253 207 81
AvaI C'yCGr_G
Cuts at: 0 965 1214 1317
Size: 965 249 103
Fragments arranged by size:
965 249 103
BamHI G'GATC_C
Cuts at: 0 816 1317
Size: 816 501
BanI G'GyrC_C
Cuts at: 0 991 1317
Size: 991 326
BanII G_rGCy'C
Cuts at: 0 154 528 985 1111 1317
Size: 154 374 457 126 206
Fragments arranged by size:
457 374 206 154 126
BbeI G_GCGC'C
Cuts at: 0 995 1317
Size: 995 322
BbsI GAAGACnn'nnnn_
Cuts at: 0 671 1317
Size: 671 646
BglI GCCn_nnn'nGGC
Cuts at: 0 532 631 989 1317
Size: 532 99 358 328
Fragments arranged by size:
532 358 328 99
Bme1580I G_kGCm'C
Cuts at: 0 154 528 767 1111 1317
Size: 154 374 239 344 206
Fragments arranged by size:
374 344 239 206 154
BmrI ACTGGGnnnn_n'
Cuts at: 0 157 444 1317
Size: 157 287 873
Fragments arranged by size:
873 287 157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 297 367 705 725 732 1317
Size: 49 248 70 338 20 7 585
Fragments arranged by size:
585 338 248 70 49 20 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1167 1317
Size: 1167 150
BsaI GGTCTCn'nnnn_
Cuts at: 0 115 287 631 1317
Size: 115 172 344 686
Fragments arranged by size:
686 344 172 115
BsaHI Gr'CG_yC
Cuts at: 0 992 1317
Size: 992 325
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 22 52 455 485 1317
Size: 22 30 403 30 832
Fragments arranged by size:
832 403 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 562 910 1274 1317
Size: 133 429 348 364 43
Fragments arranged by size:
429 364 348 133 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 493 1317
Size: 60 433 824
Fragments arranged by size:
824 433 60
BsiHKAI G_wGCw'C
Cuts at: 0 241 1317
Size: 241 1076
BsmI GAATG_Cn'
Cuts at: 0 660 1317
Size: 660 657
Bsp1286I G_dGCh'C
Cuts at: 0 154 241 528 767 985 1111 1317
Size: 154 87 287 239 218 126 206
Fragments arranged by size:
287 239 218 206 154 126 87
BsrBI CCG'CTC
Cuts at: 0 1075 1317
Size: 1075 242
BsrFI r'CCGG_y
Cuts at: 0 1155 1317
Size: 1155 162
BssHII G'CGCG_C
Cuts at: 0 161 1317
Size: 161 1156
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 434 690 1015 1317
Size: 12 422 256 325 302
Fragments arranged by size:
422 325 302 256 12
BstYI r'GATC_y
Cuts at: 0 500 816 1317
Size: 500 316 501
Fragments arranged by size:
501 500 316
Bsu36I CC'TnA_GG
Cuts at: 0 821 956 1317
Size: 821 135 361
Fragments arranged by size:
821 361 135
BtgI C'CryG_G
Cuts at: 0 11 659 917 1046 1317
Size: 11 648 258 129 271
Fragments arranged by size:
648 271 258 129 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 31 1317
Size: 31 1286
BtsI GCAGTG_nn'
Cuts at: 0 473 767 1317
Size: 473 294 550
Fragments arranged by size:
550 473 294
DraI TTT'AAA
Cuts at: 0 320 1317
Size: 320 997
EaeI y'GGCC_r
Cuts at: 0 1017 1317
Size: 1017 300
EarI CTCTTCn'nnn_
Cuts at: 0 182 1317
Size: 182 1135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 364 1317
Size: 364 953
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 297 367 705 725 732 1317
Size: 49 248 70 338 20 7 585
Fragments arranged by size:
585 338 248 70 49 20 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 28 211 1317
Size: 28 183 1106
Fragments arranged by size:
1106 183 28
EcoO109I rG'GnC_Cy
Cuts at: 0 68 135 524 940 1108 1209 1317
Size: 68 67 389 416 168 101 108
Fragments arranged by size:
416 389 168 108 101 68 67
EcoRI G'AATT_C
Cuts at: 0 253 334 541 1317
Size: 253 81 207 776
Fragments arranged by size:
776 253 207 81
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1119 1317
Size: 1119 198
HaeII r_GCGC'y
Cuts at: 0 995 1317
Size: 995 322
HincII GTy'rAC
Cuts at: 0 1131 1317
Size: 1131 186
KasI G'GCGC_C
Cuts at: 0 991 1317
Size: 991 326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 818 1317
Size: 818 499
MscI TGG'CCA
Cuts at: 0 1019 1317
Size: 1019 298
MslI CAynn'nnrTG
Cuts at: 0 16 1317
Size: 16 1301
MspA1I CmG'CkG
Cuts at: 0 538 1317
Size: 538 779
NarI GG'CG_CC
Cuts at: 0 992 1317
Size: 992 325
NcoI C'CATG_G
Cuts at: 0 11 917 1317
Size: 11 906 400
Fragments arranged by size:
906 400 11
PflMI CCAn_nnn'nTGG
Cuts at: 0 650 1317
Size: 650 667
PfoI T'CCnGG_A
Cuts at: 0 338 721 1317
Size: 338 383 596
Fragments arranged by size:
596 383 338
PpuMI rG'GwC_Cy
Cuts at: 0 68 135 940 1317
Size: 68 67 805 377
Fragments arranged by size:
805 377 68 67
PspOMI G'GGCC_C
Cuts at: 0 150 524 1107 1317
Size: 150 374 583 210
Fragments arranged by size:
583 374 210 150
SanDI GG'GwC_CC
Cuts at: 0 940 1317
Size: 940 377
SfcI C'TryA_G
Cuts at: 0 288 1317
Size: 288 1029
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 532 1317
Size: 532 785
SfoI GGC'GCC
Cuts at: 0 993 1317
Size: 993 324
SmaI CCC'GGG
Cuts at: 0 967 1317
Size: 967 350
SmlI C'TyrA_G
Cuts at: 0 1182 1317
Size: 1182 135
SrfI GCCC'GGGC
Cuts at: 0 967 1317
Size: 967 350
StyI C'CwwG_G
Cuts at: 0 11 917 1317
Size: 11 906 400
Fragments arranged by size:
906 400 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 817 1044 1317
Size: 817 227 273
Fragments arranged by size:
817 273 227
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 22 38 54 70 455 487 1317
Size: 22 16 16 16 385 32 830
Fragments arranged by size:
830 385 32 22 16 16 16
XmaI C'CCGG_G
Cuts at: 0 965 1317
Size: 965 352
XmnI GAAnn'nnTTC
Cuts at: 0 334 1317
Size: 334 983
Enzymes that do cut:
ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI
BglI Bme1580I BmrI BpmI BpuEI BsaI BsaHI BsaXI
BseRI BsgI BsiHKAI BsmI Bsp1286I BsrBI BsrFI BssHII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
HincII KasI MmeI MscI MslI MspA1I NarI NcoI
PflMI PfoI PpuMI PspOMI SanDI SfcI SfiI SfoI
SmaI SmlI SrfI StyI TaqII TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I
BsaAI BsaBI BsaWI BseYI BsiEI BsiWI BsmBI BspEI
BspHI BspMI BsrDI BsrGI BssSI BstAPI BstBI BstEII
BstZ17I ClaI CspCI DraIII DrdI EagI EcoICRI EcoRV
FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SgfI SgrAI
SnaBI SpeI SphI SspI StuI SwaI TatI Tth111I
XbaI XcmI XhoI ZraI