(Circular) (Six-base) MAPSORT of: pFN21AF0141.seq Check: 6297 from: 1 to: 8662 pFN21AF0141 8662 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5154 5814 5154 Size: 660 8002 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6472 278 Size: 53 83 186 936 4936 2468 Fragments arranged by size: 4936 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6005 1937 Size: 4068 4594 Acc65I G'GTAC_C Cuts at: 2857 5983 2857 Size: 3126 5536 AclI AA'CG_TT Cuts at: 1563 5961 6789 7162 1563 Size: 4398 828 373 3063 Fragments arranged by size: 4398 3063 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2433 3589 5048 5588 5777 6725 8109 8377 Size: 801 1156 1459 540 189 948 1384 268 Cuts at: 8377 1632 Size: 1917 Fragments arranged by size: 1917 1459 1384 1156 948 801 540 268 189 AfeI AGC'GCT Cuts at: 4046 4885 5690 4046 Size: 839 805 7018 Fragments arranged by size: 7018 839 805 AflII C'TTAA_G Cuts at: 820 1017 3382 820 Size: 197 2365 6100 Fragments arranged by size: 6100 2365 197 AflIII A'CryG_T Cuts at: 1203 5271 7561 8538 1203 Size: 4068 2290 977 1327 Fragments arranged by size: 4068 2290 1327 977 AgeI A'CCGG_T Cuts at: 7493 7493 Size: 8662 AhdI GACnn_n'nnGTC Cuts at: 5272 7391 5272 Size: 2119 6543 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8662 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4128 1184 Size: 2944 5718 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4415 4447 4415 Size: 32 8630 AlwNI CAG_nnn'CTG Cuts at: 3017 3110 3170 3844 4174 4225 4492 4642 5401 Size: 93 60 674 330 51 267 150 759 Cuts at: 5401 5473 5936 6405 7977 3017 Size: 72 463 469 1572 3702 Fragments arranged by size: 3702 1572 759 674 469 463 330 267 150 93 72 60 51 ApaI G_GGCC'C Cuts at: 2237 2466 5102 2237 Size: 229 2636 5797 Fragments arranged by size: 5797 2636 229 ApaLI G'TGCA_C Cuts at: 6719 7875 6719 Size: 1156 7506 ApoI r'AATT_y Cuts at: 1493 5971 6197 6349 8458 1493 Size: 4478 226 152 2109 1697 Fragments arranged by size: 4478 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7216 160 Size: 872 6184 1606 Fragments arranged by size: 6184 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8662 AvaI C'yCGr_G Cuts at: 1870 1943 4239 4614 5104 5987 1870 Size: 73 2296 375 490 883 4545 Fragments arranged by size: 4545 2296 883 490 375 73 BamHI G'GATC_C Cuts at: 5992 6359 5992 Size: 367 8295 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2279 2723 2857 3586 3709 Size: 325 217 627 492 444 134 729 123 Cuts at: 3709 5983 7438 618 Size: 2274 1455 1842 Fragments arranged by size: 2274 1842 1455 729 627 492 444 325 217 134 123 BanII G_rGCy'C Cuts at: 1391 2237 2328 2458 2466 2579 2741 3053 3409 Size: 846 91 130 8 113 162 312 356 Cuts at: 3409 3560 3623 3641 4168 4585 5102 5213 5828 Size: 151 63 18 527 417 517 111 615 Cuts at: 5828 5981 1391 Size: 153 4072 Fragments arranged by size: 4072 846 615 527 517 417 356 312 162 153 151 130 113 111 91 63 18 8 BbeI G_GCGC'C Cuts at: 2727 2727 Size: 8662 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4724 5576 928 Size: 971 2825 852 4014 Fragments arranged by size: 4014 2825 971 852 BbvCI CC'TCA_GC Cuts at: 5046 5937 5046 Size: 891 7771 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6853 6887 1903 Size: 34 4916 34 3678 Fragments arranged by size: 4916 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6554 7764 6554 Size: 1210 7452 BclI T'GATC_A Cuts at: 1548 1548 Size: 8662 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2347 3351 3500 Size: 107 122 71 631 605 675 1004 149 Cuts at: 3500 4244 5106 5155 7273 136 Size: 744 862 49 2118 1525 Fragments arranged by size: 2118 1525 1004 862 744 675 631 605 149 122 107 71 49 BglII A'GATC_T Cuts at: 8657 8657 Size: 8662 BlpI GC'TnA_GC Cuts at: 2942 3617 4509 4844 5022 5624 6079 2942 Size: 675 892 335 178 602 455 5525 Fragments arranged by size: 5525 892 675 602 455 335 178 Bme1580I G_kGCm'C Cuts at: 1790 2237 2282 2466 2850 3538 4182 4264 4297 Size: 447 45 184 384 688 644 82 33 Cuts at: 4297 5102 5461 6723 7879 1790 Size: 805 359 1262 1156 2573 Fragments arranged by size: 2573 1262 1156 805 688 644 447 384 359 184 82 45 33 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8662 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2814 3581 3657 Size: 416 53 83 399 24 1397 767 76 Cuts at: 3657 6393 7351 442 Size: 2736 958 1753 Fragments arranged by size: 2736 1753 1397 958 767 416 399 83 76 53 24 BmtI G_CTAG'C Cuts at: 1056 4148 1056 Size: 3092 5570 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2773 3535 2773 Size: 762 7900 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2201 2232 2652 2725 2745 2857 3147 3182 Size: 962 31 420 73 20 112 290 35 Cuts at: 3182 3216 3676 3794 3866 4115 4418 5225 5423 Size: 34 460 118 72 249 303 807 198 Cuts at: 5423 7322 1239 Size: 1899 2579 Fragments arranged by size: 2579 1899 962 807 460 420 303 290 249 198 118 112 73 72 35 34 31 20 Bpu10I CC'TnA_GC Cuts at: 4167 4584 5046 5937 4167 Size: 417 462 891 6892 Fragments arranged by size: 6892 891 462 417 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4049 4178 5696 6787 7652 7950 4049 Size: 129 1518 1091 865 298 4761 Fragments arranged by size: 4761 1518 1091 865 298 129 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3842 4946 5219 7325 882 Size: 617 2343 1104 273 2106 2219 Fragments arranged by size: 2343 2219 2106 1104 617 273 BsaAI yAC'GTr Cuts at: 493 1204 4183 5617 6453 493 Size: 711 2979 1434 836 2702 Fragments arranged by size: 2979 2702 1434 836 711 BsaBI GATnn'nnATC Cuts at: 8656 8656 Size: 8662 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2724 6469 6851 7486 Size: 53 83 186 936 1191 3745 382 635 Cuts at: 7486 275 Size: 1451 Fragments arranged by size: 3745 1451 1191 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4497 5547 7095 7493 7767 7914 8325 4497 Size: 1050 1548 398 274 147 411 4834 Fragments arranged by size: 4834 1548 1050 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8437 Fragments arranged by size: 8437 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2639 4016 4085 4478 4481 4801 4804 4819 Size: 1453 1377 69 393 3 320 3 15 Cuts at: 4819 4822 4825 5186 5324 5420 5423 1186 Size: 3 3 361 138 96 3 4425 Fragments arranged by size: 4425 1453 1377 393 361 320 138 96 69 15 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2972 3058 3103 3212 3486 3529 3622 3902 3914 Size: 86 45 109 274 43 93 280 12 Cuts at: 3914 4095 4174 4568 4839 5092 5833 7865 8361 Size: 181 79 394 271 253 741 2032 496 Cuts at: 8361 2972 Size: 3273 Fragments arranged by size: 3273 2032 741 496 394 280 274 271 253 181 109 93 86 79 45 43 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 2296 4096 4613 5597 5835 1163 Size: 573 345 215 1800 517 984 238 3990 Fragments arranged by size: 3990 1800 984 573 517 345 238 215 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6112 6873 7901 1540 Size: 275 186 4111 761 1028 2301 Fragments arranged by size: 4111 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2256 2579 2923 3118 3409 4094 4107 5184 5213 Size: 323 344 195 291 685 13 1077 29 Cuts at: 5213 5625 6723 6808 7879 2256 Size: 412 1098 85 1071 3039 Fragments arranged by size: 3039 1098 1077 1071 685 412 344 323 291 195 85 29 13 BsmI GAATG_Cn' Cuts at: 3135 4630 6173 6266 3135 Size: 1495 1543 93 5531 Fragments arranged by size: 5531 1543 1495 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5741 5741 Size: 8662 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2256 2282 2328 2458 2466 2579 Size: 399 447 19 26 46 130 8 113 Cuts at: 2579 2741 2850 2923 3053 3118 3409 3538 3560 Size: 162 109 73 130 65 291 129 22 Cuts at: 3560 3623 3641 4094 4107 4168 4182 4264 4297 Size: 63 18 453 13 61 14 82 33 Cuts at: 4297 4585 5102 5184 5213 5461 5625 5828 5981 Size: 288 517 82 29 248 164 203 153 Cuts at: 5981 6723 6808 7879 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 517 453 447 399 291 288 248 203 164 162 153 130 130 129 113 109 85 82 82 73 65 63 61 46 33 29 26 22 19 18 14 13 8 BspEI T'CCGG_A Cuts at: 4497 5547 8325 4497 Size: 1050 2778 4834 Fragments arranged by size: 4834 2778 1050 BspHI T'CATG_A Cuts at: 5955 6551 8281 5955 Size: 596 1730 6336 Fragments arranged by size: 6336 1730 596 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2280 2601 2706 2869 5154 5419 5547 5615 Size: 1436 321 105 163 2285 265 128 68 Cuts at: 5615 5814 6017 844 Size: 199 203 3489 Fragments arranged by size: 3489 2285 1436 321 265 203 199 163 128 105 68 BsrBI CCG'CTC Cuts at: 1394 1823 3244 3727 3802 4162 4258 4291 4447 Size: 429 1421 483 75 360 96 33 156 Cuts at: 4447 4749 5374 5413 6549 1394 Size: 302 625 39 1136 3507 Fragments arranged by size: 3507 1421 1136 625 483 429 360 302 156 96 75 39 33 BsrDI GCAATG_nn' Cuts at: 65 1248 2311 7157 7331 65 Size: 1183 1063 4846 174 1396 Fragments arranged by size: 4846 1396 1183 1063 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3345 3783 4481 5352 5706 5865 7306 Size: 110 1535 438 698 871 354 159 1441 Cuts at: 7306 7493 1700 Size: 187 2869 Fragments arranged by size: 2869 1535 1441 871 698 438 354 187 159 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8662 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8662 BssSI C'ACGA_G Cuts at: 3966 6722 7734 3966 Size: 2756 1012 4894 Fragments arranged by size: 4894 2756 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2310 2370 4225 5620 5637 794 Size: 1516 60 1855 1395 17 3819 Fragments arranged by size: 3819 1855 1516 1395 60 17 BstBI TT'CG_AA Cuts at: 7466 7466 Size: 8662 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8662 BstXI CCAn_nnnn'nTGG Cuts at: 2734 2734 Size: 8662 BstYI r'GATC_y Cuts at: 1972 3439 4575 5992 6359 6744 6761 8213 8299 Size: 1467 1136 1417 367 385 17 1452 86 Cuts at: 8299 8311 8657 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1467 1452 1417 1136 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3897 3897 Size: 8662 BtgI C'CryG_G Cuts at: 513 1065 2004 2055 3496 5593 5641 513 Size: 552 939 51 1441 2097 48 3534 Fragments arranged by size: 3534 2097 1441 939 552 51 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4510 487 Size: 683 56 3284 4639 Fragments arranged by size: 4639 3284 683 56 BtsI GCAGTG_nn' Cuts at: 2149 2254 2800 3244 4262 4295 4326 4937 5818 Size: 105 546 444 1018 33 31 611 881 Cuts at: 5818 6178 6972 6992 2149 Size: 360 794 20 3819 Fragments arranged by size: 3819 1018 881 794 611 546 444 360 105 33 31 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8662 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8627 DraI TTT'AAA Cuts at: 5966 5966 Size: 8662 DraIII CAC_nnn'GTG Cuts at: 2131 2822 3575 5620 2131 Size: 691 753 2045 5173 Fragments arranged by size: 5173 2045 753 691 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7669 809 Size: 507 6353 1802 Fragments arranged by size: 6353 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3081 4479 4483 Size: 54 1425 50 275 18 1251 1398 4 Cuts at: 4483 4548 6109 6998 8627 8 Size: 65 1561 889 1629 43 Fragments arranged by size: 1629 1561 1425 1398 1251 889 275 65 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 6109 1537 Size: 275 4297 4090 Fragments arranged by size: 4297 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2785 4152 4828 4995 6592 1353 Size: 1432 1367 676 167 1597 3423 Fragments arranged by size: 3423 1597 1432 1367 676 167 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3041 4006 5431 7249 7623 7769 Size: 1064 138 1452 965 1425 1818 374 146 Cuts at: 7769 387 Size: 1280 Fragments arranged by size: 1818 1452 1425 1280 1064 965 374 146 138 EcoICRI GAG'CTC Cuts at: 2577 3407 5211 2577 Size: 830 1804 6028 Fragments arranged by size: 6028 1804 830 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2232 2433 2652 2725 2745 2857 Size: 393 569 31 201 219 73 20 112 Cuts at: 2857 3147 3182 3216 3589 3676 3794 3866 4115 Size: 290 35 34 373 87 118 72 249 Cuts at: 4115 4418 5048 5225 5423 5588 5777 6725 7322 Size: 303 630 177 198 165 189 948 597 Cuts at: 7322 8109 8377 1239 Size: 787 268 1524 Fragments arranged by size: 1524 948 787 630 597 569 393 373 303 290 268 249 219 201 198 189 177 165 118 112 87 73 72 35 34 31 20 EcoNI CCTnn'n_nnAGG Cuts at: 4736 5405 4736 Size: 669 7993 EcoO109I rG'GnC_Cy Cuts at: 2033 2069 2233 2463 2540 2590 2837 3352 3893 Size: 36 164 230 77 50 247 515 541 Cuts at: 3893 3960 3981 4393 5099 5398 2033 Size: 67 21 412 706 299 5297 Fragments arranged by size: 5297 706 541 515 412 299 247 230 164 77 67 50 36 21 EcoRI G'AATT_C Cuts at: 5971 6349 5971 Size: 378 8284 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3619 5575 3619 Size: 1956 6706 FseI GG_CCGG'CC Cuts at: 4485 4485 Size: 8662 FspI TGC'GCA Cuts at: 5810 7168 5810 Size: 1358 7304 HaeII r_GCGC'y Cuts at: 1678 2695 2727 3487 3796 3844 4048 4546 4887 Size: 1017 32 760 309 48 204 498 341 Cuts at: 4887 4963 5692 7809 1678 Size: 76 729 2117 2531 Fragments arranged by size: 2531 2117 1017 760 729 498 341 309 204 76 48 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4567 4599 5258 5290 7303 Size: 32 135 32 2995 32 659 32 2013 Cuts at: 7303 7335 7377 7409 1373 Size: 32 42 32 2626 Fragments arranged by size: 2995 2626 2013 659 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3125 6006 6252 669 Size: 988 281 1187 2881 246 3079 Fragments arranged by size: 3079 2881 1187 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8662 HpaI GTT'AAC Cuts at: 6252 6252 Size: 8662 KasI G'GCGC_C Cuts at: 2723 2723 Size: 8662 KpnI G_GTAC'C Cuts at: 2861 5987 2861 Size: 3126 5536 MfeI C'AATT_G Cuts at: 6261 6261 Size: 8662 MluI A'CGCG_T Cuts at: 8538 8538 Size: 8662 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4422 7776 7960 1305 Size: 3117 3354 184 2007 Fragments arranged by size: 3354 3117 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3083 4550 10 Size: 54 1425 343 1251 1467 4122 Fragments arranged by size: 4122 1467 1425 1251 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2732 4110 5187 6620 6979 7138 518 Size: 648 1566 1378 1077 1433 359 159 2042 Fragments arranged by size: 2042 1566 1433 1378 1077 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2179 2854 3070 3418 3696 4225 4330 4639 Size: 573 675 216 348 278 529 105 309 Cuts at: 4639 4797 5008 5045 5096 5473 6078 6755 7903 Size: 158 211 37 51 377 605 677 1148 Cuts at: 7903 8148 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 675 605 573 529 377 348 309 278 245 216 211 158 105 51 37 NaeI GCC'GGC Cuts at: 3347 4483 3347 Size: 1136 7526 NarI GG'CG_CC Cuts at: 2724 2724 Size: 8662 NcoI C'CATG_G Cuts at: 513 1065 2004 2055 3496 5593 513 Size: 552 939 51 1441 2097 3582 Fragments arranged by size: 3582 2097 1441 939 552 51 NdeI CA'TA_TG Cuts at: 387 387 Size: 8662 NgoMIV G'CCGG_C Cuts at: 3345 4481 3345 Size: 1136 7526 NheI G'CTAG_C Cuts at: 1052 4144 1052 Size: 3092 5570 NotI GC'GGCC_GC Cuts at: 6109 6109 Size: 8662 NspI r_CATG'y Cuts at: 1132 1229 2368 5275 6020 7565 1132 Size: 97 1139 2907 745 1545 2229 Fragments arranged by size: 2907 2229 1545 1139 745 97 PasI CC'CwG_GG Cuts at: 1348 2459 4858 1348 Size: 1111 2399 5152 Fragments arranged by size: 5152 2399 1111 PciI A'CATG_T Cuts at: 5271 7561 5271 Size: 2290 6372 PflMI CCAn_nnn'nTGG Cuts at: 1108 2056 3110 3277 3844 4441 1108 Size: 948 1054 167 567 597 5329 Fragments arranged by size: 5329 1054 948 597 567 167 PfoI T'CCnGG_A Cuts at: 3198 3771 3807 4554 3198 Size: 573 36 747 7306 Fragments arranged by size: 7306 747 573 36 PmeI GTTT'AAAC Cuts at: 5966 5966 Size: 8662 PmlI CAC'GTG Cuts at: 4183 5617 4183 Size: 1434 7228 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6731 6763 8244 8276 6731 Size: 32 1481 32 7117 Fragments arranged by size: 7117 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2069 2540 2590 2837 3893 2069 Size: 471 50 247 1056 6838 Fragments arranged by size: 6838 1056 471 247 50 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8662 PsiI TTA'TAA Cuts at: 6232 6232 Size: 8662 PspOMI G'GGCC_C Cuts at: 2233 2462 5098 2233 Size: 229 2636 5797 Fragments arranged by size: 5797 2636 229 PstI C_TGCA'G Cuts at: 830 2212 2488 3013 3321 4230 4272 4305 4335 Size: 1382 276 525 308 909 42 33 30 Cuts at: 4335 4944 4977 5448 5896 6014 830 Size: 609 33 471 448 118 3478 Fragments arranged by size: 3478 1382 909 609 525 471 448 308 276 118 42 33 33 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8662 PvuII CAG'CTG Cuts at: 3070 4225 4330 4639 5045 5473 3070 Size: 1155 105 309 406 428 6259 Fragments arranged by size: 6259 1155 428 406 309 105 SacI G_AGCT'C Cuts at: 2579 3409 5213 2579 Size: 830 1804 6028 Fragments arranged by size: 6028 1804 830 SalI G'TCGA_C Cuts at: 1936 6004 1936 Size: 4068 4594 SanDI GG'GwC_CC Cuts at: 2540 2540 Size: 8662 SapI GCTCTTCn'nnn_ Cuts at: 4152 4828 4995 4152 Size: 676 167 7819 Fragments arranged by size: 7819 676 167 SbfI CC_TGCA'GG Cuts at: 6014 6014 Size: 8662 ScaI AGT'ACT Cuts at: 6910 8622 6910 Size: 1712 6950 SexAI A'CCwGG_T Cuts at: 2127 2127 Size: 8662 SfcI C'TryA_G Cuts at: 826 1045 2208 2228 2470 2484 2891 3009 3317 Size: 219 1163 20 242 14 407 118 308 Cuts at: 3317 3628 4226 4268 4301 4331 4940 4973 5444 Size: 311 598 42 33 30 609 33 471 Cuts at: 5444 5892 6010 7145 7826 8017 826 Size: 448 118 1135 681 191 1471 Fragments arranged by size: 1471 1163 1135 681 609 598 471 448 407 311 308 242 219 191 118 118 42 33 33 30 20 14 SfiI GGCCn_nnn'nGGCC Cuts at: 3351 3351 Size: 8662 SfoI GGC'GCC Cuts at: 2725 2725 Size: 8662 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8662 SgrAI Cr'CCGG_yG Cuts at: 1700 3783 1700 Size: 2083 6579 SmaI CCC'GGG Cuts at: 1872 4616 5989 1872 Size: 2744 1373 4545 Fragments arranged by size: 4545 2744 1373 SmlI C'TyrA_G Cuts at: 820 1017 1943 3382 4064 4193 5711 6766 7667 Size: 197 926 1439 682 129 1518 1055 901 Cuts at: 7667 7929 820 Size: 262 1553 Fragments arranged by size: 1553 1518 1439 1055 926 901 682 262 197 129 SnaBI TAC'GTA Cuts at: 493 6453 493 Size: 5960 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8662 SphI G_CATG'C Cuts at: 1132 6020 1132 Size: 4888 3774 SspI AAT'ATT Cuts at: 5 52 6586 5 Size: 47 6534 2081 Fragments arranged by size: 6534 2081 47 StuI AGG'CCT Cuts at: 1517 2241 2968 3972 4708 1517 Size: 724 727 1004 736 5471 Fragments arranged by size: 5471 1004 736 727 724 StyI C'CwwG_G Cuts at: 513 1065 2004 2055 2073 3496 4350 5593 6101 Size: 552 939 51 18 1423 854 1243 508 Cuts at: 6101 513 Size: 3074 Fragments arranged by size: 3074 1423 1243 939 854 552 508 51 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2833 3050 3563 3580 4034 4109 4910 Size: 1255 1253 217 513 17 454 75 801 Cuts at: 4910 6689 6706 6859 7044 7489 325 Size: 1779 17 153 185 445 1498 Fragments arranged by size: 1779 1498 1255 1253 801 513 454 445 217 185 153 75 17 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5793 5871 6908 Size: 274 80 33 51 1443 3816 78 1037 Cuts at: 6908 8620 96 Size: 1712 138 Fragments arranged by size: 3816 1712 1443 1037 274 138 80 78 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2829 2861 3121 3153 3424 3456 4977 5009 8622 Size: 32 260 32 271 32 1521 32 3613 Cuts at: 8622 8654 2829 Size: 32 2837 Fragments arranged by size: 3613 2837 1521 271 260 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8662 XbaI T'CTAG_A Cuts at: 5998 5998 Size: 8662 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2033 2881 3937 2033 Size: 848 1056 6758 Fragments arranged by size: 6758 1056 848 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8662 XmaI C'CCGG_G Cuts at: 1870 4614 5987 1870 Size: 2744 1373 4545 Fragments arranged by size: 4545 2744 1373 XmnI GAAnn'nnTTC Cuts at: 3289 6791 3289 Size: 3502 5160 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6470 276 Size: 53 83 186 936 4936 2468 Fragments arranged by size: 4936 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SrfI SwaI