(Circular) (Six-base) MAPSORT of: pFN21AB6116.seq Check: 8434 from: 1 to: 6451 pFN21AB6116 6451 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2464 2464 Size: 6451 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4261 278 Size: 53 83 186 936 2725 2468 Fragments arranged by size: 2725 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3696 3794 1937 Size: 1759 98 4594 Fragments arranged by size: 4594 1759 98 Acc65I G'GTAC_C Cuts at: 2148 3772 2148 Size: 1624 4827 AclI AA'CG_TT Cuts at: 1563 4578 4951 1563 Size: 3015 373 3063 Fragments arranged by size: 3063 3015 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3731 4514 5898 6166 1632 Size: 2099 783 1384 268 1917 Fragments arranged by size: 2099 1917 1384 783 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6254 AflIII A'CryG_T Cuts at: 1203 3651 5350 6327 1203 Size: 2448 1699 977 1327 Fragments arranged by size: 2448 1699 1327 977 AgeI A'CCGG_T Cuts at: 5282 5282 Size: 6451 AhdI GACnn_n'nnGTC Cuts at: 5180 5180 Size: 6451 AleI CACnn'nnGTG Cuts at: 1166 2267 1166 Size: 1101 5350 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2504 3443 1184 Size: 1320 939 4192 Fragments arranged by size: 4192 1320 939 AlwNI CAG_nnn'CTG Cuts at: 2062 2166 2656 2992 4194 5766 2062 Size: 104 490 336 1202 1572 2747 Fragments arranged by size: 2747 1572 1202 490 336 104 ApaI G_GGCC'C Cuts at: 2021 2123 3277 2021 Size: 102 1154 5195 Fragments arranged by size: 5195 1154 102 ApaLI G'TGCA_C Cuts at: 2786 4508 5664 2786 Size: 1722 1156 3573 Fragments arranged by size: 3573 1722 1156 ApoI r'AATT_y Cuts at: 1493 3760 3986 4138 6247 1493 Size: 2267 226 152 2109 1697 Fragments arranged by size: 2267 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5005 160 Size: 872 3973 1606 Fragments arranged by size: 3973 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6451 AvaI C'yCGr_G Cuts at: 1870 1943 2172 2975 3293 3405 3456 3776 1870 Size: 73 229 803 318 112 51 320 4545 Fragments arranged by size: 4545 803 320 318 229 112 73 51 BamHI G'GATC_C Cuts at: 3781 4148 3781 Size: 367 6084 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2148 2292 2552 2567 2903 Size: 325 217 627 361 144 260 15 336 Cuts at: 2903 2999 3242 3772 5227 618 Size: 96 243 530 1455 1842 Fragments arranged by size: 1842 1455 627 530 361 336 325 260 243 217 144 96 15 BanII G_rGCy'C Cuts at: 1391 2021 2123 2403 2829 3277 3406 3705 3750 Size: 630 102 280 426 448 129 299 45 Cuts at: 3750 3770 1391 Size: 20 4072 Fragments arranged by size: 4072 630 448 426 299 280 129 102 45 20 BbeI G_GCGC'C Cuts at: 2296 2296 Size: 6451 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2469 3531 928 Size: 971 570 1062 3848 Fragments arranged by size: 3848 1062 971 570 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2557 2591 2893 2927 4642 4676 1903 Size: 34 620 34 302 34 1715 34 3678 Fragments arranged by size: 3678 1715 620 302 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4343 5553 4343 Size: 1210 5241 BclI T'GATC_A Cuts at: 1548 2634 1548 Size: 1086 5365 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2111 2325 3336 Size: 107 122 71 631 605 439 214 1011 Cuts at: 3336 5062 136 Size: 1726 1525 Fragments arranged by size: 1726 1525 1011 631 605 439 214 122 107 71 BglII A'GATC_T Cuts at: 6446 6446 Size: 6451 BlpI GC'TnA_GC Cuts at: 3868 3868 Size: 6451 Bme1580I G_kGCm'C Cuts at: 1790 2021 2123 2790 3277 3375 4512 5668 1790 Size: 231 102 667 487 98 1137 1156 2573 Fragments arranged by size: 2573 1156 1137 667 487 231 102 98 BmgBI CAC'GTC Cuts at: 1318 2770 2785 1318 Size: 1452 15 4984 Fragments arranged by size: 4984 1452 15 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4182 5140 442 Size: 416 53 83 399 24 2765 958 1753 Fragments arranged by size: 2765 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6451 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3586 3586 Size: 6451 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2694 2843 3087 5111 1239 Size: 1455 149 244 2024 2579 Fragments arranged by size: 2579 2024 1455 244 149 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3573 3689 4576 5441 5739 3573 Size: 116 887 865 298 4285 Fragments arranged by size: 4285 887 865 298 116 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3167 3179 5114 882 Size: 617 1668 12 1935 2219 Fragments arranged by size: 2219 1935 1668 617 12 BsaAI yAC'GTr Cuts at: 493 1204 4242 493 Size: 711 3038 2702 Fragments arranged by size: 3038 2702 711 BsaBI GATnn'nnATC Cuts at: 2693 6445 2693 Size: 3752 2699 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2293 2350 3610 4258 Size: 53 83 186 936 760 57 1260 648 Cuts at: 4258 4640 5275 275 Size: 382 635 1451 Fragments arranged by size: 1451 1260 936 760 648 635 382 186 83 57 53 BsaWI w'CCGG_w Cuts at: 2410 3559 4884 5282 5556 5703 6114 2410 Size: 1149 1325 398 274 147 411 2747 Fragments arranged by size: 2747 1325 1149 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2555 2585 2873 2903 3437 Size: 30 165 30 1152 30 288 30 534 Cuts at: 3437 3467 1178 Size: 30 4162 Fragments arranged by size: 4162 1152 534 288 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 3578 3743 3758 1186 Size: 841 1551 165 15 3879 Fragments arranged by size: 3879 1551 841 165 15 BseYI C'CCAG_C Cuts at: 2024 2095 2659 3246 5654 6150 2024 Size: 71 564 587 2408 496 2325 Fragments arranged by size: 2408 2325 587 564 496 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2096 2201 2492 1163 Size: 573 360 105 291 5122 Fragments arranged by size: 5122 573 360 291 105 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2115 3901 4662 5690 1540 Size: 275 186 114 1786 761 1028 2301 Fragments arranged by size: 2301 1786 1028 761 275 186 114 BsiHKAI G_wGCw'C Cuts at: 2483 2790 2829 3705 3750 4512 4597 5668 2483 Size: 307 39 876 45 762 85 1071 3266 Fragments arranged by size: 3266 1071 876 762 307 85 45 39 BsmI GAATG_Cn' Cuts at: 3962 4055 3962 Size: 93 6358 BsmBI CGTCTCn'nnnn_ Cuts at: 2810 2810 Size: 6451 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2123 2403 2483 2790 2829 3277 Size: 399 231 102 280 80 307 39 448 Cuts at: 3277 3375 3406 3705 3750 3770 4512 4597 5668 Size: 98 31 299 45 20 742 85 1071 Cuts at: 5668 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 448 399 307 299 280 231 102 98 85 80 45 39 31 20 BspEI T'CCGG_A Cuts at: 3559 6114 3559 Size: 2555 3896 BspHI T'CATG_A Cuts at: 4340 6070 4340 Size: 1730 4721 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2464 3185 3806 844 Size: 1620 721 621 3489 Fragments arranged by size: 3489 1620 721 621 BsrBI CCG'CTC Cuts at: 1394 1823 2344 3733 4338 1394 Size: 429 521 1389 605 3507 Fragments arranged by size: 3507 1389 605 521 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2483 3187 4946 5120 65 Size: 1183 1235 704 1759 174 1396 Fragments arranged by size: 1759 1396 1235 1183 704 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5095 5282 1700 Size: 110 3285 187 2869 Fragments arranged by size: 3285 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6451 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6451 BssSI C'ACGA_G Cuts at: 3740 4511 5523 3740 Size: 771 1012 4668 Fragments arranged by size: 4668 1012 771 BstAPI GCAn_nnn'nTGC Cuts at: 794 2062 794 Size: 1268 5183 BstBI TT'CG_AA Cuts at: 5255 5255 Size: 6451 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6451 BstXI CCAn_nnnn'nTGG Cuts at: 2281 2549 2885 2281 Size: 268 336 5847 Fragments arranged by size: 5847 336 268 BstYI r'GATC_y Cuts at: 1972 3075 3781 4148 4533 4550 6002 6088 6100 Size: 1103 706 367 385 17 1452 86 12 Cuts at: 6100 6446 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1103 706 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2013 2082 3388 513 Size: 552 948 69 1306 3576 Fragments arranged by size: 3576 1306 948 552 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5712 Fragments arranged by size: 5712 683 56 BtsI GCAGTG_nn' Cuts at: 2500 3427 3967 4761 4781 2500 Size: 927 540 794 20 4170 Fragments arranged by size: 4170 927 794 540 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6451 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3178 3213 529 Size: 35 2614 35 3767 Fragments arranged by size: 3767 2614 35 35 DraI TTT'AAA Cuts at: 3755 3755 Size: 6451 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2615 5458 809 Size: 507 1299 2843 1802 Fragments arranged by size: 2843 1802 1299 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2112 2271 3164 Size: 54 1425 50 275 18 282 159 893 Cuts at: 3164 3898 4787 6416 8 Size: 734 889 1629 43 Fragments arranged by size: 1629 1425 893 889 734 282 275 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2112 3898 1537 Size: 275 300 1786 4090 Fragments arranged by size: 4090 1786 300 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2915 4381 1353 Size: 1562 1466 3423 Fragments arranged by size: 3423 1562 1466 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5038 5412 5558 387 Size: 1064 138 3449 374 146 1280 Fragments arranged by size: 3449 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2827 3703 3748 2827 Size: 876 45 5530 Fragments arranged by size: 5530 876 45 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2694 2843 3087 3731 4514 5111 5898 Size: 393 1062 149 244 644 783 597 787 Cuts at: 5898 6166 1239 Size: 268 1524 Fragments arranged by size: 1524 1062 787 783 644 597 393 268 244 149 EcoNI CCTnn'n_nnAGG Cuts at: 3539 3539 Size: 6451 EcoO109I rG'GnC_Cy Cuts at: 2018 2119 2120 2163 3273 3274 3502 3553 3628 Size: 101 1 43 1110 1 228 51 75 Cuts at: 3628 2018 Size: 4841 Fragments arranged by size: 4841 1110 228 101 75 51 43 1 1 EcoRI G'AATT_C Cuts at: 3760 4138 3760 Size: 378 6073 FspI TGC'GCA Cuts at: 4957 4957 Size: 6451 HaeII r_GCGC'y Cuts at: 1678 2296 5598 1678 Size: 618 3302 2531 Fragments arranged by size: 3302 2531 618 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2555 2587 3060 3092 3437 Size: 32 135 32 983 32 473 32 345 Cuts at: 3437 3469 5092 5124 5166 5198 1373 Size: 32 1623 32 42 32 2626 Fragments arranged by size: 2626 1623 983 473 345 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2338 3697 3795 4041 669 Size: 988 281 400 1359 98 246 3079 Fragments arranged by size: 3079 1359 988 400 281 246 98 HindIII A'AGCT_T Cuts at: 748 3194 748 Size: 2446 4005 HpaI GTT'AAC Cuts at: 4041 4041 Size: 6451 KasI G'GCGC_C Cuts at: 2292 2292 Size: 6451 KpnI G_GTAC'C Cuts at: 2152 3776 2152 Size: 1624 4827 MfeI C'AATT_G Cuts at: 4050 4050 Size: 6451 MluI A'CGCG_T Cuts at: 6327 6327 Size: 6451 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5565 5749 1305 Size: 4260 184 2007 Fragments arranged by size: 4260 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2273 10 Size: 54 1425 343 441 4188 Fragments arranged by size: 4188 1425 441 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2267 2279 2306 2327 2547 2883 3344 Size: 648 1101 12 27 21 220 336 461 Cuts at: 3344 4409 4768 4927 518 Size: 1065 359 159 2042 Fragments arranged by size: 2042 1101 1065 648 461 359 336 220 159 27 21 12 MspA1I CmG'CkG Cuts at: 1606 2015 3250 3867 4544 5692 5937 1606 Size: 409 1235 617 677 1148 245 2120 Fragments arranged by size: 2120 1235 1148 677 617 409 245 NarI GG'CG_CC Cuts at: 2293 2293 Size: 6451 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5899 NdeI CA'TA_TG Cuts at: 387 387 Size: 6451 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6451 NotI GC'GGCC_GC Cuts at: 3898 3898 Size: 6451 NspI r_CATG'y Cuts at: 1132 1229 2332 2668 3343 3655 3809 5354 1132 Size: 97 1103 336 675 312 154 1545 2229 Fragments arranged by size: 2229 1545 1103 675 336 312 154 97 PasI CC'CwG_GG Cuts at: 1348 2160 1348 Size: 812 5639 PciI A'CATG_T Cuts at: 3651 5350 3651 Size: 1699 4752 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6451 PfoI T'CCnGG_A Cuts at: 2517 2671 2517 Size: 154 6297 PmeI GTTT'AAAC Cuts at: 3755 3755 Size: 6451 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4520 4552 6033 6065 4520 Size: 32 1481 32 4906 Fragments arranged by size: 4906 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3502 3553 3628 3502 Size: 51 75 6325 Fragments arranged by size: 6325 75 51 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6451 PsiI TTA'TAA Cuts at: 4021 4021 Size: 6451 PspOMI G'GGCC_C Cuts at: 2017 2119 3273 2017 Size: 102 1154 5195 Fragments arranged by size: 5195 1154 102 PstI C_TGCA'G Cuts at: 830 2058 2997 3336 3803 830 Size: 1228 939 339 467 3478 Fragments arranged by size: 3478 1228 939 467 339 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6451 PvuII CAG'CTG Cuts at: 3250 3250 Size: 6451 SacI G_AGCT'C Cuts at: 2829 3705 3750 2829 Size: 876 45 5530 Fragments arranged by size: 5530 876 45 SacII CC_GC'GG Cuts at: 2016 2016 Size: 6451 SalI G'TCGA_C Cuts at: 1936 3695 3793 1936 Size: 1759 98 4594 Fragments arranged by size: 4594 1759 98 SbfI CC_TGCA'GG Cuts at: 3336 3803 3336 Size: 467 5984 ScaI AGT'ACT Cuts at: 4699 6411 4699 Size: 1712 4739 SfcI C'TryA_G Cuts at: 826 1045 2054 2587 2993 3332 3799 4934 5615 Size: 219 1009 533 406 339 467 1135 681 Cuts at: 5615 5806 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1009 681 533 467 406 339 219 191 SfoI GGC'GCC Cuts at: 2294 2294 Size: 6451 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6451 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6451 SmaI CCC'GGG Cuts at: 1872 2174 2977 3407 3458 3778 1872 Size: 302 803 430 51 320 4545 Fragments arranged by size: 4545 803 430 320 302 51 SmlI C'TyrA_G Cuts at: 820 1017 1943 3588 3704 4555 5456 5718 820 Size: 197 926 1645 116 851 901 262 1553 Fragments arranged by size: 1645 1553 926 901 851 262 197 116 SnaBI TAC'GTA Cuts at: 493 4242 493 Size: 3749 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6451 SphI G_CATG'C Cuts at: 1132 3809 1132 Size: 2677 3774 SspI AAT'ATT Cuts at: 5 52 4375 5 Size: 47 4323 2081 Fragments arranged by size: 4323 2081 47 StuI AGG'CCT Cuts at: 1517 2319 2753 3318 1517 Size: 802 434 565 4650 Fragments arranged by size: 4650 802 565 434 StyI C'CwwG_G Cuts at: 513 1065 2265 3890 513 Size: 552 1200 1625 3074 Fragments arranged by size: 3074 1625 1200 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2273 2328 3260 4478 4495 4648 4833 Size: 1255 693 55 932 1218 17 153 185 Cuts at: 4833 5278 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1218 932 693 445 185 153 55 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4697 6409 96 Size: 274 80 33 51 1443 2720 1712 138 Fragments arranged by size: 2720 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6411 6443 6411 Size: 32 6419 Tth111I GACn'n_nGTC Cuts at: 1316 2531 2804 3157 1316 Size: 1215 273 353 4610 Fragments arranged by size: 4610 1215 353 273 XbaI T'CTAG_A Cuts at: 3787 3787 Size: 6451 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2375 3395 2375 Size: 1020 5431 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6451 XmaI C'CCGG_G Cuts at: 1870 2172 2975 3405 3456 3776 1870 Size: 302 803 430 51 320 4545 Fragments arranged by size: 4545 803 430 320 302 51 XmnI GAAnn'nnTTC Cuts at: 4580 4580 Size: 6451 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4259 276 Size: 53 83 186 936 2725 2468 Fragments arranged by size: 2725 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI