(Circular) (Six-base) MAPSORT of: pFN21AB6365.seq Check: 3012 from: 1 to: 5509 pFN21AB6365 5509 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3319 278 Size: 53 83 186 936 1783 2468 Fragments arranged by size: 2468 1783 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2852 1937 Size: 915 4594 Acc65I G'GTAC_C Cuts at: 2830 2830 Size: 5509 AclI AA'CG_TT Cuts at: 1563 3636 4009 1563 Size: 2073 373 3063 Fragments arranged by size: 3063 2073 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2020 3572 4956 5224 1632 Size: 388 1552 1384 268 1917 Fragments arranged by size: 1917 1552 1384 388 268 AfeI AGC'GCT Cuts at: 2161 2161 Size: 5509 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5312 AflIII A'CryG_T Cuts at: 1203 4408 5385 1203 Size: 3205 977 1327 Fragments arranged by size: 3205 1327 977 AgeI A'CCGG_T Cuts at: 4340 4340 Size: 5509 AhdI GACnn_n'nnGTC Cuts at: 4238 4238 Size: 5509 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5509 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5509 AlwNI CAG_nnn'CTG Cuts at: 2058 3252 4824 2058 Size: 1194 1572 2743 Fragments arranged by size: 2743 1572 1194 ApaLI G'TGCA_C Cuts at: 3566 4722 3566 Size: 1156 4353 ApoI r'AATT_y Cuts at: 1493 2423 2818 3044 3196 5305 1493 Size: 930 395 226 152 2109 1697 Fragments arranged by size: 2109 1697 930 395 226 152 AseI AT'TA_AT Cuts at: 160 1032 4063 160 Size: 872 3031 1606 Fragments arranged by size: 3031 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5509 AvaI C'yCGr_G Cuts at: 1870 1943 2254 2616 2834 1870 Size: 73 311 362 218 4545 Fragments arranged by size: 4545 362 311 218 73 BamHI G'GATC_C Cuts at: 2336 2839 3206 2336 Size: 503 367 4639 Fragments arranged by size: 4639 503 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2259 2830 4285 618 Size: 325 217 627 472 571 1455 1842 Fragments arranged by size: 1842 1455 627 571 472 325 217 BanII G_rGCy'C Cuts at: 1391 2065 2252 2828 1391 Size: 674 187 576 4072 Fragments arranged by size: 4072 674 576 187 BbeI G_GCGC'C Cuts at: 2263 2263 Size: 5509 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2160 928 Size: 971 261 4277 Fragments arranged by size: 4277 971 261 BbvCI CC'TCA_GC Cuts at: 2051 2530 2051 Size: 479 5030 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3700 3734 1903 Size: 34 1763 34 3678 Fragments arranged by size: 3678 1763 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3401 4611 3401 Size: 1210 4299 BclI T'GATC_A Cuts at: 1548 1548 Size: 5509 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2268 4120 136 Size: 107 122 71 631 605 596 1852 1525 Fragments arranged by size: 1852 1525 631 605 596 122 107 71 BglII A'GATC_T Cuts at: 2310 5504 2310 Size: 3194 2315 BlpI GC'TnA_GC Cuts at: 2926 2926 Size: 5509 Bme1580I G_kGCm'C Cuts at: 1790 3570 4726 1790 Size: 1780 1156 2573 Fragments arranged by size: 2573 1780 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5509 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3240 4198 442 Size: 416 53 83 399 24 1823 958 1753 Fragments arranged by size: 1823 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5509 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2119 4169 1239 Size: 880 2050 2579 Fragments arranged by size: 2579 2050 880 Bpu10I CC'TnA_GC Cuts at: 2051 2530 2051 Size: 479 5030 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2777 3634 4499 4797 2777 Size: 857 865 298 3489 Fragments arranged by size: 3489 865 857 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4172 882 Size: 617 2673 2219 Fragments arranged by size: 2673 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3300 493 Size: 711 2096 2702 Fragments arranged by size: 2702 2096 711 BsaBI GATnn'nnATC Cuts at: 5503 5503 Size: 5509 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2260 3316 3698 4333 Size: 53 83 186 936 727 1056 382 635 Cuts at: 4333 275 Size: 1451 Fragments arranged by size: 1451 1056 936 727 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3942 4340 4614 4761 5172 3942 Size: 398 274 147 411 4279 Fragments arranged by size: 4279 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5284 Fragments arranged by size: 5284 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2012 2015 2018 2173 2176 2421 1186 Size: 826 3 3 155 3 245 4274 Fragments arranged by size: 4274 826 245 155 3 3 3 BseYI C'CCAG_C Cuts at: 2628 4712 5208 2628 Size: 2084 496 2929 Fragments arranged by size: 2929 2084 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2629 2739 1163 Size: 573 893 110 3933 Fragments arranged by size: 3933 893 573 110 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2959 3720 4748 1540 Size: 275 186 958 761 1028 2301 Fragments arranged by size: 2301 1028 958 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3570 3655 4726 3570 Size: 85 1071 4353 Fragments arranged by size: 4353 1071 85 BsmI GAATG_Cn' Cuts at: 3020 3113 3020 Size: 93 5416 BsmBI CGTCTCn'nnnn_ Cuts at: 2084 2084 Size: 5509 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2065 2252 2828 3570 3655 4726 1391 Size: 399 275 187 576 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 576 399 275 187 85 BspEI T'CCGG_A Cuts at: 5172 5172 Size: 5509 BspHI T'CATG_A Cuts at: 3398 5128 3398 Size: 1730 3779 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2864 844 Size: 2020 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3396 1394 Size: 429 1573 3507 Fragments arranged by size: 3507 1573 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4004 4178 65 Size: 1183 2756 174 1396 Fragments arranged by size: 2756 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2267 4153 4340 1700 Size: 110 457 1886 187 2869 Fragments arranged by size: 2869 1886 457 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5509 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5509 BssSI C'ACGA_G Cuts at: 3569 4581 3569 Size: 1012 4497 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5509 BstBI TT'CG_AA Cuts at: 4313 4313 Size: 5509 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5509 BstXI CCAn_nnnn'nTGG Cuts at: 2219 2219 Size: 5509 BstYI r'GATC_y Cuts at: 1972 2310 2336 2839 3206 3591 3608 5060 5146 Size: 338 26 503 367 385 17 1452 86 Cuts at: 5146 5158 5504 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 503 385 367 346 338 86 26 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4957 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2447 487 Size: 683 56 1221 3549 Fragments arranged by size: 3549 1221 683 56 BtsI GCAGTG_nn' Cuts at: 3025 3819 3839 3025 Size: 794 20 4695 Fragments arranged by size: 4695 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5509 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5474 DraI TTT'AAA Cuts at: 2813 2813 Size: 5509 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4516 809 Size: 507 3200 1802 Fragments arranged by size: 3200 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2956 3845 5474 Size: 54 1425 50 275 18 1126 889 1629 Cuts at: 5474 8 Size: 43 Fragments arranged by size: 1629 1425 1126 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2956 1537 Size: 275 1144 4090 Fragments arranged by size: 4090 1144 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2036 2179 3439 1353 Size: 683 143 1260 3423 Fragments arranged by size: 3423 1260 683 143 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4096 4470 4616 387 Size: 1064 138 2507 374 146 1280 Fragments arranged by size: 2507 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2020 2119 3572 4169 4956 5224 1239 Size: 393 388 99 1453 597 787 268 1524 Fragments arranged by size: 1524 1453 787 597 393 388 268 99 EcoRI G'AATT_C Cuts at: 2818 3196 2818 Size: 378 5131 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2818 2818 Size: 5509 FspI TGC'GCA Cuts at: 4015 4015 Size: 5509 HaeII r_GCGC'y Cuts at: 1678 2058 2163 2263 4656 1678 Size: 380 105 100 2393 2531 Fragments arranged by size: 2531 2393 380 105 100 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2277 2309 4150 4182 4224 Size: 32 135 32 705 32 1841 32 42 Cuts at: 4224 4256 1373 Size: 32 2626 Fragments arranged by size: 2626 1841 705 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2853 3099 669 Size: 988 281 915 246 3079 Fragments arranged by size: 3079 988 915 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5509 HpaI GTT'AAC Cuts at: 3099 3099 Size: 5509 KasI G'GCGC_C Cuts at: 2259 2259 Size: 5509 KpnI G_GTAC'C Cuts at: 2834 2834 Size: 5509 MfeI C'AATT_G Cuts at: 3108 3108 Size: 5509 MluI A'CGCG_T Cuts at: 5385 5385 Size: 5509 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2649 4623 4807 1305 Size: 1344 1974 184 2007 Fragments arranged by size: 2007 1974 1344 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3687 Fragments arranged by size: 3687 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2460 3467 3826 3985 518 Size: 648 1294 1007 359 159 2042 Fragments arranged by size: 2042 1294 1007 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2108 2925 3602 4750 4995 1606 Size: 502 817 677 1148 245 2120 Fragments arranged by size: 2120 1148 817 677 502 245 NarI GG'CG_CC Cuts at: 2260 2260 Size: 5509 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4957 NdeI CA'TA_TG Cuts at: 387 387 Size: 5509 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5509 NotI GC'GGCC_GC Cuts at: 2956 2956 Size: 5509 NspI r_CATG'y Cuts at: 1132 1229 2867 4412 1132 Size: 97 1638 1545 2229 Fragments arranged by size: 2229 1638 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5509 PciI A'CATG_T Cuts at: 4408 4408 Size: 5509 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5509 PmeI GTTT'AAAC Cuts at: 2813 2813 Size: 5509 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3578 3610 5091 5123 3578 Size: 32 1481 32 3964 Fragments arranged by size: 3964 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5509 PsiI TTA'TAA Cuts at: 3079 3079 Size: 5509 PstI C_TGCA'G Cuts at: 830 2285 2310 2861 830 Size: 1455 25 551 3478 Fragments arranged by size: 3478 1455 551 25 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5509 RsrII CG'GwC_CG Cuts at: 2265 2265 Size: 5509 SalI G'TCGA_C Cuts at: 1936 2851 1936 Size: 915 4594 SbfI CC_TGCA'GG Cuts at: 2861 2861 Size: 5509 ScaI AGT'ACT Cuts at: 3757 5469 3757 Size: 1712 3797 SfcI C'TryA_G Cuts at: 826 1045 2281 2306 2857 3992 4673 4864 826 Size: 219 1236 25 551 1135 681 191 1471 Fragments arranged by size: 1471 1236 1135 681 551 219 191 25 SfoI GGC'GCC Cuts at: 2261 2261 Size: 5509 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5509 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5509 SmaI CCC'GGG Cuts at: 1872 2618 2836 1872 Size: 746 218 4545 Fragments arranged by size: 4545 746 218 SmlI C'TyrA_G Cuts at: 820 1017 1943 2792 3613 4514 4776 820 Size: 197 926 849 821 901 262 1553 Fragments arranged by size: 1553 926 901 849 821 262 197 SnaBI TAC'GTA Cuts at: 493 3300 493 Size: 2807 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5509 SphI G_CATG'C Cuts at: 1132 2867 1132 Size: 1735 3774 SspI AAT'ATT Cuts at: 5 52 2373 3433 5 Size: 47 2321 1060 2081 Fragments arranged by size: 2321 2081 1060 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5509 StyI C'CwwG_G Cuts at: 513 1065 2212 2948 513 Size: 552 1147 736 3074 Fragments arranged by size: 3074 1147 736 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3536 3553 3706 3891 4336 325 Size: 1255 1956 17 153 185 445 1498 Fragments arranged by size: 1956 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3755 5467 96 Size: 274 80 33 51 1443 1778 1712 138 Fragments arranged by size: 1778 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5469 5501 5469 Size: 32 5477 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5509 XbaI T'CTAG_A Cuts at: 2845 2845 Size: 5509 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5509 XmaI C'CCGG_G Cuts at: 1870 2616 2834 1870 Size: 746 218 4545 Fragments arranged by size: 4545 746 218 XmnI GAAnn'nnTTC Cuts at: 3638 3638 Size: 5509 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3317 276 Size: 53 83 186 936 1783 2468 Fragments arranged by size: 2468 1783 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI RsrII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI