Restriction map for
pFN21AB6365
(Linear) (Six-base) MAPSORT of: KIBB6365.seq Check: 3944 from: 1 to: 804
KIBB6365.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 804
Size: 15 789
AfeI AGC'GCT
Cuts at: 0 156 804
Size: 156 648
AlwNI CAG_nnn'CTG
Cuts at: 0 53 804
Size: 53 751
ApoI r'AATT_y
Cuts at: 0 418 804
Size: 418 386
AvaI C'yCGr_G
Cuts at: 0 249 611 804
Size: 249 362 193
Fragments arranged by size:
362 249 193
BamHI G'GATC_C
Cuts at: 0 331 804
Size: 331 473
BanI G'GyrC_C
Cuts at: 0 254 804
Size: 254 550
BanII G_rGCy'C
Cuts at: 0 60 247 804
Size: 60 187 557
Fragments arranged by size:
557 187 60
BbeI G_GCGC'C
Cuts at: 0 258 804
Size: 258 546
BbsI GAAGACnn'nnnn_
Cuts at: 0 155 804
Size: 155 649
BbvCI CC'TCA_GC
Cuts at: 0 46 525 804
Size: 46 479 279
Fragments arranged by size:
479 279 46
BglI GCCn_nnn'nGGC
Cuts at: 0 263 804
Size: 263 541
BglII A'GATC_T
Cuts at: 0 305 804
Size: 305 499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 804
Size: 114 690
Bpu10I CC'TnA_GC
Cuts at: 0 46 525 804
Size: 46 479 279
Fragments arranged by size:
479 279 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 804
Size: 772 32
BsaHI Gr'CG_yC
Cuts at: 0 255 804
Size: 255 549
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 10 13 168 171 416 804
Size: 7 3 3 155 3 245 388
Fragments arranged by size:
388 245 155 7 3 3 3
BseYI C'CCAG_C
Cuts at: 0 623 804
Size: 623 181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 624 734 804
Size: 624 110 70
Fragments arranged by size:
624 110 70
BsmBI CGTCTCn'nnnn_
Cuts at: 0 79 804
Size: 79 725
Bsp1286I G_dGCh'C
Cuts at: 0 60 247 804
Size: 60 187 557
Fragments arranged by size:
557 187 60
BsrFI r'CCGG_y
Cuts at: 0 262 804
Size: 262 542
BstXI CCAn_nnnn'nTGG
Cuts at: 0 214 804
Size: 214 590
BstYI r'GATC_y
Cuts at: 0 305 331 804
Size: 305 26 473
Fragments arranged by size:
473 305 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 442 804
Size: 442 362
EarI CTCTTCn'nnn_
Cuts at: 0 31 174 804
Size: 31 143 630
Fragments arranged by size:
630 143 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 114 804
Size: 15 99 690
Fragments arranged by size:
690 99 15
HaeII r_GCGC'y
Cuts at: 0 53 158 258 804
Size: 53 105 100 546
Fragments arranged by size:
546 105 100 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 272 304 804
Size: 272 32 500
Fragments arranged by size:
500 272 32
KasI G'GCGC_C
Cuts at: 0 254 804
Size: 254 550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 804
Size: 644 160
MslI CAynn'nnrTG
Cuts at: 0 455 804
Size: 455 349
MspA1I CmG'CkG
Cuts at: 0 103 804
Size: 103 701
NarI GG'CG_CC
Cuts at: 0 255 804
Size: 255 549
PstI C_TGCA'G
Cuts at: 0 280 305 804
Size: 280 25 499
Fragments arranged by size:
499 280 25
RsrII CG'GwC_CG
Cuts at: 0 260 804
Size: 260 544
SfcI C'TryA_G
Cuts at: 0 276 301 804
Size: 276 25 503
Fragments arranged by size:
503 276 25
SfoI GGC'GCC
Cuts at: 0 256 804
Size: 256 548
SmaI CCC'GGG
Cuts at: 0 613 804
Size: 613 191
SmlI C'TyrA_G
Cuts at: 0 787 804
Size: 787 17
SspI AAT'ATT
Cuts at: 0 368 804
Size: 368 436
StyI C'CwwG_G
Cuts at: 0 207 804
Size: 207 597
XmaI C'CCGG_G
Cuts at: 0 611 804
Size: 611 193
Enzymes that do cut:
AcuI AfeI AlwNI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BglI BglII BpmI Bpu10I BpuEI
BsaHI BseRI BseYI BsgI BsmBI Bsp1286I BsrFI BstXI
BstYI BtgZI EarI Eco57MI HaeII Hin4I KasI MmeI
MslI MspA1I NarI PstI RsrII SfcI SfoI SmaI
SmlI SspI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BlpI
Bme1580I BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI
BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MscI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI StuI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI