Restriction map for
pFN21AB5050
(Linear) (Six-base) MAPSORT of: KIBB5050.seq Check: 786 from: 1 to: 1881
KIBB5050.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 0 1119 1359 1881
Size: 1119 240 522
Fragments arranged by size:
1119 522 240
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 725 1856 1881
Size: 220 505 1131 25
Fragments arranged by size:
1131 505 220 25
AflIII A'CryG_T
Cuts at: 0 679 1242 1364 1484 1881
Size: 679 563 122 120 397
Fragments arranged by size:
679 563 397 122 120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1649 1881
Size: 1649 232
AlwNI CAG_nnn'CTG
Cuts at: 0 162 1108 1348 1881
Size: 162 946 240 533
Fragments arranged by size:
946 533 240 162
ApoI r'AATT_y
Cuts at: 0 695 1723 1881
Size: 695 1028 158
Fragments arranged by size:
1028 695 158
AvaI C'yCGr_G
Cuts at: 0 9 1193 1881
Size: 9 1184 688
Fragments arranged by size:
1184 688 9
BamHI G'GATC_C
Cuts at: 0 1338 1881
Size: 1338 543
BanI G'GyrC_C
Cuts at: 0 1496 1881
Size: 1496 385
BbsI GAAGACnn'nnnn_
Cuts at: 0 713 1881
Size: 713 1168
BciVI GTATCCnnnnn_n'
Cuts at: 0 1423 1881
Size: 1423 458
BclI T'GATC_A
Cuts at: 0 900 908 1881
Size: 900 8 973
Fragments arranged by size:
973 900 8
BfrBI ATG'CAT
Cuts at: 0 1161 1881
Size: 1161 720
Bme1580I G_kGCm'C
Cuts at: 0 190 1499 1881
Size: 190 1309 382
Fragments arranged by size:
1309 382 190
BmgBI CAC'GTC
Cuts at: 0 95 1881
Size: 95 1786
BmrI ACTGGGnnnn_n'
Cuts at: 0 359 576 1881
Size: 359 217 1305
Fragments arranged by size:
1305 359 217
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 312 883 1448 1881
Size: 312 571 565 433
Fragments arranged by size:
571 565 433 312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 510 711 741 873 1130 1881
Size: 445 65 201 30 132 257 751
Fragments arranged by size:
751 445 257 201 132 65 30
Bpu10I CC'TnA_GC
Cuts at: 0 100 1881
Size: 100 1781
BsaHI Gr'CG_yC
Cuts at: 0 426 1881
Size: 426 1455
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 875 1881
Size: 875 1006
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1832 1881
Size: 1832 49
BsmI GAATG_Cn'
Cuts at: 0 1104 1478 1557 1881
Size: 1104 374 79 324
Fragments arranged by size:
1104 374 324 79
Bsp1286I G_dGCh'C
Cuts at: 0 190 1499 1881
Size: 190 1309 382
Fragments arranged by size:
1309 382 190
BspHI T'CATG_A
Cuts at: 0 1167 1881
Size: 1167 714
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 668 1881
Size: 668 1213
BsrBI CCG'CTC
Cuts at: 0 296 1881
Size: 296 1585
BsrDI GCAATG_nn'
Cuts at: 0 604 757 1606 1881
Size: 604 153 849 275
Fragments arranged by size:
849 604 275 153
BsrFI r'CCGG_y
Cuts at: 0 63 1881
Size: 63 1818
BstBI TT'CG_AA
Cuts at: 0 1731 1881
Size: 1731 150
BstEII G'GTnAC_C
Cuts at: 0 931 1881
Size: 931 950
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1851 1881
Size: 1851 30
BstYI r'GATC_y
Cuts at: 0 1069 1338 1429 1881
Size: 1069 269 91 452
Fragments arranged by size:
1069 452 269 91
BstZ17I GTA'TAC
Cuts at: 0 1120 1881
Size: 1120 761
Bsu36I CC'TnA_GG
Cuts at: 0 878 1881
Size: 878 1003
BtgI C'CryG_G
Cuts at: 0 279 808 1881
Size: 279 529 1073
Fragments arranged by size:
1073 529 279
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 336 371 1881
Size: 336 35 1510
Fragments arranged by size:
1510 336 35
DraI TTT'AAA
Cuts at: 0 968 1881
Size: 968 913
EaeI y'GGCC_r
Cuts at: 0 408 1881
Size: 408 1473
EarI CTCTTCn'nnn_
Cuts at: 0 734 1881
Size: 734 1147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1794 1881
Size: 1794 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 445 510 711 725 741 873 1130
Size: 220 225 65 201 14 16 132 257
Cuts at: 1130 1856 1881
Size: 726 25
Fragments arranged by size:
726 257 225 220 201 132 65 25
16 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 140 1881
Size: 140 1741
EcoO109I rG'GnC_Cy
Cuts at: 0 390 1615 1881
Size: 390 1225 266
Fragments arranged by size:
1225 390 266
EcoRV GAT'ATC
Cuts at: 0 1581 1881
Size: 1581 300
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 730 1881
Size: 730 1151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1040 1072 1881
Size: 1040 32 809
Fragments arranged by size:
1040 809 32
HincII GTy'rAC
Cuts at: 0 1490 1508 1881
Size: 1490 18 373
Fragments arranged by size:
1490 373 18
HpaI GTT'AAC
Cuts at: 0 1490 1508 1881
Size: 1490 18 373
Fragments arranged by size:
1490 373 18
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 1804 1881
Size: 254 1550 77
Fragments arranged by size:
1550 254 77
MscI TGG'CCA
Cuts at: 0 410 1881
Size: 410 1471
MslI CAynn'nnrTG
Cuts at: 0 1022 1166 1631 1881
Size: 1022 144 465 250
Fragments arranged by size:
1022 465 250 144
MspA1I CmG'CkG
Cuts at: 0 1274 1881
Size: 1274 607
NcoI C'CATG_G
Cuts at: 0 279 808 1881
Size: 279 529 1073
Fragments arranged by size:
1073 529 279
NdeI CA'TA_TG
Cuts at: 0 560 1314 1881
Size: 560 754 567
Fragments arranged by size:
754 567 560
NsiI A_TGCA'T
Cuts at: 0 1163 1881
Size: 1163 718
NspI r_CATG'y
Cuts at: 0 108 683 917 1246 1881
Size: 108 575 234 329 635
Fragments arranged by size:
635 575 329 234 108
PciI A'CATG_T
Cuts at: 0 679 1242 1881
Size: 679 563 639
Fragments arranged by size:
679 639 563
PfoI T'CCnGG_A
Cuts at: 0 461 644 1881
Size: 461 183 1237
Fragments arranged by size:
1237 461 183
PpuMI rG'GwC_Cy
Cuts at: 0 390 1615 1881
Size: 390 1225 266
Fragments arranged by size:
1225 390 266
SexAI A'CCwGG_T
Cuts at: 0 789 1881
Size: 789 1092
SfcI C'TryA_G
Cuts at: 0 452 1073 1562 1881
Size: 452 621 489 319
Fragments arranged by size:
621 489 452 319
SmlI C'TyrA_G
Cuts at: 0 1193 1881
Size: 1193 688
SspI AAT'ATT
Cuts at: 0 1536 1881
Size: 1536 345
StuI AGG'CCT
Cuts at: 0 458 1881
Size: 458 1423
StyI C'CwwG_G
Cuts at: 0 279 411 808 1881
Size: 279 132 397 1073
Fragments arranged by size:
1073 397 279 132
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1423 1881
Size: 1423 458
XhoI C'TCGA_G
Cuts at: 0 1193 1881
Size: 1193 688
XmnI GAAnn'nnTTC
Cuts at: 0 1727 1881
Size: 1727 154
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApoI AvaI BamHI
BanI BbsI BciVI BclI BfrBI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaHI BseRI BsgI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI CspCI DraI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I HincII
HpaI MmeI MscI MslI MspA1I NcoI NdeI NsiI
NspI PciI PfoI PpuMI SexAI SfcI SmlI SspI
StuI StyI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BanII BbeI BbvCI BcgI BglI BglII BlpI
BmtI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseYI
BsiEI BsiHKAI BsiWI BsmBI BspEI BsrGI BssHII BssSI
BstAPI BtgZI BtsI ClaI DraIII DrdI EagI EcoICRI
EcoRI FseI FspI FspAI HaeII HindIII KasI KpnI
MfeI MluI NaeI NarI NgoMIV NheI NotI NruI
PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
TaqII TatI TstI Tth111I XbaI XmaI ZraI