(Circular) (Six-base) MAPSORT of: pFN21AB6517.seq Check: 5677 from: 1 to: 5791 pFN21AB6517 5791 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3601 278 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2065 3134 1937 Size: 128 1069 4594 Fragments arranged by size: 4594 1069 128 Acc65I G'GTAC_C Cuts at: 3112 3112 Size: 5791 AclI AA'CG_TT Cuts at: 1563 3918 4291 1563 Size: 2355 373 3063 Fragments arranged by size: 3063 2355 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3049 3854 5238 5506 1632 Size: 1417 805 1384 268 1917 Fragments arranged by size: 1917 1417 1384 805 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5594 AflIII A'CryG_T Cuts at: 1203 4690 5667 1203 Size: 3487 977 1327 Fragments arranged by size: 3487 1327 977 AgeI A'CCGG_T Cuts at: 4622 4622 Size: 5791 AhdI GACnn_n'nnGTC Cuts at: 2811 4520 2811 Size: 1709 4082 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5791 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5791 AlwNI CAG_nnn'CTG Cuts at: 3534 5106 3534 Size: 1572 4219 ApaLI G'TGCA_C Cuts at: 2019 3848 5004 2019 Size: 1829 1156 2806 Fragments arranged by size: 2806 1829 1156 ApoI r'AATT_y Cuts at: 1493 2752 3012 3055 3100 3326 3478 5587 1493 Size: 1259 260 43 45 226 152 2109 1697 Fragments arranged by size: 2109 1697 1259 260 226 152 45 43 AseI AT'TA_AT Cuts at: 160 1032 4345 160 Size: 872 3313 1606 Fragments arranged by size: 3313 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 AvaI C'yCGr_G Cuts at: 1870 1943 3116 1870 Size: 73 1173 4545 Fragments arranged by size: 4545 1173 73 BamHI G'GATC_C Cuts at: 2591 3121 3488 2591 Size: 530 367 4894 Fragments arranged by size: 4894 530 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3112 4567 618 Size: 325 217 627 1325 1455 1842 Fragments arranged by size: 1842 1455 1325 627 325 217 BanII G_rGCy'C Cuts at: 1391 3110 1391 Size: 1719 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2036 2207 2612 928 Size: 971 137 171 405 4107 Fragments arranged by size: 4107 971 405 171 137 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3982 4016 1903 Size: 34 50 34 1961 34 3678 Fragments arranged by size: 3678 1961 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3683 4893 3683 Size: 1210 4581 BclI T'GATC_A Cuts at: 1548 1548 Size: 5791 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4402 136 Size: 107 122 71 631 605 2730 1525 Fragments arranged by size: 2730 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2597 2974 5786 2597 Size: 377 2812 2602 Fragments arranged by size: 2812 2602 377 BlpI GC'TnA_GC Cuts at: 3208 3208 Size: 5791 Bme1580I G_kGCm'C Cuts at: 1790 2023 2337 3852 5008 1790 Size: 233 314 1515 1156 2573 Fragments arranged by size: 2573 1515 1156 314 233 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5791 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3522 4480 442 Size: 416 53 83 399 24 2105 958 1753 Fragments arranged by size: 2105 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5791 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2262 2817 4451 1239 Size: 1023 555 1634 2579 Fragments arranged by size: 2579 1634 1023 555 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2791 3014 3916 4781 5079 2791 Size: 223 902 865 298 3503 Fragments arranged by size: 3503 902 865 298 223 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4454 882 Size: 617 2955 2219 Fragments arranged by size: 2955 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3582 493 Size: 711 2378 2702 Fragments arranged by size: 2702 2378 711 BsaBI GATnn'nnATC Cuts at: 2596 5785 2596 Size: 3189 2602 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3598 3980 4615 275 Size: 53 83 186 936 2065 382 635 1451 Fragments arranged by size: 2065 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4224 4622 4896 5043 5454 4224 Size: 398 274 147 411 4561 Fragments arranged by size: 4561 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2162 2192 1178 Size: 30 165 30 759 30 4777 Fragments arranged by size: 4777 759 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5791 BseYI C'CCAG_C Cuts at: 4994 5490 4994 Size: 496 5295 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2287 2425 2485 1163 Size: 573 551 138 60 4469 Fragments arranged by size: 4469 573 551 138 60 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3241 4002 5030 1540 Size: 275 186 1240 761 1028 2301 Fragments arranged by size: 2301 1240 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2023 3852 3937 5008 2023 Size: 1829 85 1071 2806 Fragments arranged by size: 2806 1829 1071 85 BsmI GAATG_Cn' Cuts at: 2558 3302 3395 2558 Size: 744 93 4954 Fragments arranged by size: 4954 744 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2337 3110 3852 3937 5008 1391 Size: 399 233 314 773 742 85 1071 2174 Fragments arranged by size: 2174 1071 773 742 399 314 233 85 BspEI T'CCGG_A Cuts at: 5454 5454 Size: 5791 BspHI T'CATG_A Cuts at: 2245 2713 2802 3680 5410 2245 Size: 468 89 878 1730 2626 Fragments arranged by size: 2626 1730 878 468 89 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3146 844 Size: 2302 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3678 1394 Size: 429 1855 3507 Fragments arranged by size: 3507 1855 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4286 4460 65 Size: 1183 3038 174 1396 Fragments arranged by size: 3038 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4435 4622 1700 Size: 110 2625 187 2869 Fragments arranged by size: 2869 2625 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5791 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5791 BssSI C'ACGA_G Cuts at: 3851 4863 3851 Size: 1012 4779 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5791 BstBI TT'CG_AA Cuts at: 2833 4595 2833 Size: 1762 4029 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5791 BstXI CCAn_nnnn'nTGG Cuts at: 2188 2188 Size: 5791 BstYI r'GATC_y Cuts at: 1972 2591 2597 2974 3121 3488 3873 3890 5342 Size: 619 6 377 147 367 385 17 1452 Cuts at: 5342 5428 5440 5786 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 619 385 377 367 346 147 86 17 12 6 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5239 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5052 Fragments arranged by size: 5052 683 56 BtsI GCAGTG_nn' Cuts at: 3307 4101 4121 3307 Size: 794 20 4977 Fragments arranged by size: 4977 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5791 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2094 2129 529 Size: 35 1530 35 4191 Fragments arranged by size: 4191 1530 35 35 DraI TTT'AAA Cuts at: 3095 3095 Size: 5791 DraIII CAC_nnn'GTG Cuts at: 2577 2577 Size: 5791 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2306 4798 809 Size: 507 990 2492 1802 Fragments arranged by size: 2492 1802 990 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2251 3238 4127 Size: 54 1425 50 275 18 421 987 889 Cuts at: 4127 5756 8 Size: 1629 43 Fragments arranged by size: 1629 1425 987 889 421 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3238 1537 Size: 275 1426 4090 Fragments arranged by size: 4090 1426 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2651 3721 1353 Size: 1298 1070 3423 Fragments arranged by size: 3423 1298 1070 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4378 4752 4898 387 Size: 1064 138 2789 374 146 1280 Fragments arranged by size: 2789 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2262 2817 3049 3854 4451 5238 5506 Size: 393 630 555 232 805 597 787 268 Cuts at: 5506 1239 Size: 1524 Fragments arranged by size: 1524 805 787 630 597 555 393 268 232 EcoRI G'AATT_C Cuts at: 3100 3478 3100 Size: 378 5413 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3008 3008 Size: 5791 FspI TGC'GCA Cuts at: 4297 4297 Size: 5791 HaeII r_GCGC'y Cuts at: 1678 4938 1678 Size: 3260 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2162 2194 2576 2608 2797 Size: 32 135 32 590 32 382 32 189 Cuts at: 2797 2829 2959 2991 4432 4464 4506 4538 1373 Size: 32 130 32 1441 32 42 32 2626 Fragments arranged by size: 2626 1441 590 382 189 135 130 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2767 3135 3381 669 Size: 988 281 829 368 246 3079 Fragments arranged by size: 3079 988 829 368 281 246 HindIII A'AGCT_T Cuts at: 748 3082 748 Size: 2334 3457 HpaI GTT'AAC Cuts at: 3381 3381 Size: 5791 KpnI G_GTAC'C Cuts at: 3116 3116 Size: 5791 MfeI C'AATT_G Cuts at: 3390 3390 Size: 5791 MluI A'CGCG_T Cuts at: 5667 5667 Size: 5791 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2747 4905 5089 1305 Size: 1442 2158 184 2007 Fragments arranged by size: 2158 2007 1442 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2253 10 Size: 54 1425 343 421 3548 Fragments arranged by size: 3548 1425 421 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2247 3749 4108 4267 518 Size: 648 1081 1502 359 159 2042 Fragments arranged by size: 2042 1502 1081 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3207 3884 5032 5277 1606 Size: 1601 677 1148 245 2120 Fragments arranged by size: 2120 1601 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5239 NdeI CA'TA_TG Cuts at: 387 387 Size: 5791 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5791 NotI GC'GGCC_GC Cuts at: 3238 3238 Size: 5791 NspI r_CATG'y Cuts at: 1132 1229 2682 3149 4694 1132 Size: 97 1453 467 1545 2229 Fragments arranged by size: 2229 1545 1453 467 97 PasI CC'CwG_GG Cuts at: 1348 2038 1348 Size: 690 5101 PciI A'CATG_T Cuts at: 4690 4690 Size: 5791 PflMI CCAn_nnn'nTGG Cuts at: 1108 3085 1108 Size: 1977 3814 PmeI GTTT'AAAC Cuts at: 3095 3095 Size: 5791 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3860 3892 5373 5405 3860 Size: 32 1481 32 4246 Fragments arranged by size: 4246 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5791 PsiI TTA'TAA Cuts at: 3361 3361 Size: 5791 PstI C_TGCA'G Cuts at: 830 3143 830 Size: 2313 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5791 SalI G'TCGA_C Cuts at: 1936 3133 1936 Size: 1197 4594 SbfI CC_TGCA'GG Cuts at: 3143 3143 Size: 5791 ScaI AGT'ACT Cuts at: 4039 5751 4039 Size: 1712 4079 SfcI C'TryA_G Cuts at: 826 1045 2211 3139 4274 4955 5146 826 Size: 219 1166 928 1135 681 191 1471 Fragments arranged by size: 1471 1166 1135 928 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5791 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5791 SmaI CCC'GGG Cuts at: 1872 3118 1872 Size: 1246 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2770 3029 3895 4796 5058 820 Size: 197 926 827 259 866 901 262 1553 Fragments arranged by size: 1553 926 901 866 827 262 259 197 SnaBI TAC'GTA Cuts at: 493 3582 493 Size: 3089 2702 SpeI A'CTAG_T Cuts at: 152 3041 152 Size: 2889 2902 SphI G_CATG'C Cuts at: 1132 3149 1132 Size: 2017 3774 SspI AAT'ATT Cuts at: 5 52 3715 5 Size: 47 3663 2081 Fragments arranged by size: 3663 2081 47 StuI AGG'CCT Cuts at: 1517 2238 1517 Size: 721 5070 StyI C'CwwG_G Cuts at: 513 1065 2181 2704 3230 513 Size: 552 1116 523 526 3074 Fragments arranged by size: 3074 1116 552 526 523 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2641 3818 3835 3988 4173 4618 325 Size: 1255 1061 1177 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1177 1061 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2355 2668 2996 Size: 274 80 33 51 1443 378 313 328 Cuts at: 2996 4037 5749 96 Size: 1041 1712 138 Fragments arranged by size: 1712 1443 1041 378 328 313 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5751 5783 5751 Size: 32 5759 Tth111I GACn'n_nGTC Cuts at: 1316 2882 1316 Size: 1566 4225 XbaI T'CTAG_A Cuts at: 3127 3127 Size: 5791 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2602 2646 2602 Size: 44 5747 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5791 XmaI C'CCGG_G Cuts at: 1870 3116 1870 Size: 1246 4545 XmnI GAAnn'nnTTC Cuts at: 3920 3920 Size: 5791 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3599 276 Size: 53 83 186 936 2065 2468 Fragments arranged by size: 2468 2065 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI