Restriction map for
pFN21AB6517
(Linear) (Six-base) MAPSORT of: KIBB6517.seq Check: 6221 from: 1 to: 1086
KIBB6517.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 0 60 1086
Size: 60 1026
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1044 1086
Size: 1044 42
AhdI GACnn_n'nnGTC
Cuts at: 0 806 1086
Size: 806 280
ApaLI G'TGCA_C
Cuts at: 0 14 1086
Size: 14 1072
ApoI r'AATT_y
Cuts at: 0 747 1007 1050 1086
Size: 747 260 43 36
Fragments arranged by size:
747 260 43 36
BamHI G'GATC_C
Cuts at: 0 586 1086
Size: 586 500
BbsI GAAGACnn'nnnn_
Cuts at: 0 31 202 607 1086
Size: 31 171 405 479
Fragments arranged by size:
479 405 171 31
BglII A'GATC_T
Cuts at: 0 592 969 1086
Size: 592 377 117
Fragments arranged by size:
592 377 117
Bme1580I G_kGCm'C
Cuts at: 0 18 332 1086
Size: 18 314 754
Fragments arranged by size:
754 314 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 812 1086
Size: 257 555 274
Fragments arranged by size:
555 274 257
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 786 1009 1086
Size: 786 223 77
Fragments arranged by size:
786 223 77
BsaBI GATnn'nnATC
Cuts at: 0 591 1086
Size: 591 495
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 157 187 1086
Size: 157 30 899
Fragments arranged by size:
899 157 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 420 480 1086
Size: 282 138 60 606
Fragments arranged by size:
606 282 138 60
BsiHKAI G_wGCw'C
Cuts at: 0 18 1086
Size: 18 1068
BsmI GAATG_Cn'
Cuts at: 0 553 1086
Size: 553 533
Bsp1286I G_dGCh'C
Cuts at: 0 18 332 1086
Size: 18 314 754
Fragments arranged by size:
754 314 18
BspHI T'CATG_A
Cuts at: 0 240 708 797 1086
Size: 240 468 89 289
Fragments arranged by size:
468 289 240 89
BstBI TT'CG_AA
Cuts at: 0 828 1086
Size: 828 258
BstXI CCAn_nnnn'nTGG
Cuts at: 0 183 1086
Size: 183 903
BstYI r'GATC_y
Cuts at: 0 586 592 969 1086
Size: 586 6 377 117
Fragments arranged by size:
586 377 117 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 89 124 1086
Size: 89 35 962
Fragments arranged by size:
962 89 35
DraIII CAC_nnn'GTG
Cuts at: 0 572 1086
Size: 572 514
DrdI GACnn_nn'nnGTC
Cuts at: 0 301 1086
Size: 301 785
EaeI y'GGCC_r
Cuts at: 0 246 1086
Size: 246 840
EarI CTCTTCn'nnn_
Cuts at: 0 646 1086
Size: 646 440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 812 1044 1086
Size: 257 555 232 42
Fragments arranged by size:
555 257 232 42
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1003 1086
Size: 1003 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 157 189 571 603 792 824 954 986
Size: 157 32 382 32 189 32 130 32
Cuts at: 986 1086
Size: 100
Fragments arranged by size:
382 189 157 130 100 32 32 32
32
HincII GTy'rAC
Cuts at: 0 762 1086
Size: 762 324
HindIII A'AGCT_T
Cuts at: 0 1077 1086
Size: 1077 9
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 1086
Size: 742 344
MscI TGG'CCA
Cuts at: 0 248 1086
Size: 248 838
MslI CAynn'nnrTG
Cuts at: 0 242 1086
Size: 242 844
NspI r_CATG'y
Cuts at: 0 677 1086
Size: 677 409
PasI CC'CwG_GG
Cuts at: 0 33 1086
Size: 33 1053
PflMI CCAn_nnn'nTGG
Cuts at: 0 1080 1086
Size: 1080 6
SfcI C'TryA_G
Cuts at: 0 206 1086
Size: 206 880
SmlI C'TyrA_G
Cuts at: 0 765 1024 1086
Size: 765 259 62
Fragments arranged by size:
765 259 62
SpeI A'CTAG_T
Cuts at: 0 1036 1086
Size: 1036 50
StuI AGG'CCT
Cuts at: 0 233 1086
Size: 233 853
StyI C'CwwG_G
Cuts at: 0 176 699 1086
Size: 176 523 387
Fragments arranged by size:
523 387 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 636 1086
Size: 636 450
TatI w'GTAC_w
Cuts at: 0 350 663 991 1086
Size: 350 313 328 95
Fragments arranged by size:
350 328 313 95
Tth111I GACn'n_nGTC
Cuts at: 0 877 1086
Size: 877 209
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 597 641 1086
Size: 597 44 445
Fragments arranged by size:
597 445 44
Enzymes that do cut:
AccI AcuI AhdI ApaLI ApoI BamHI BbsI BglII
Bme1580I BpmI BpuEI BsaBI BsaXI BsgI BsiHKAI BsmI
Bsp1286I BspHI BstBI BstXI BstYI CspCI DraIII DrdI
EaeI EarI Eco57MI FalI Hin4I HincII HindIII MmeI
MscI MslI NspI PasI PflMI SfcI SmlI SpeI
StuI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BseYI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI DraI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HpaI
KasI KpnI MfeI MluI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI SspI SwaI
TstI XbaI XhoI XmaI XmnI ZraI