Restriction map for
pFN21AE0487
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanII BbrPI BcgI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BseRI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeI MfeI MluI MluNI MscI
MunI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 946 CGCG CG^CG
AfaI 552 GTAC GT^AC
AspHI 772 GAGCAC G[AT]GC[AT]^C
BanI 421 GGCGCC G^G[CT][GA]CC
BbeI 421 GGCGCC GGCGC^C
BbsI 802 GAAGAC GAAGAC(2/6)
BbvCI 402 CCTCAGC CCTCAGC(-5/-2)
BciVI 856 GTATCC GTATCC(6/5)
BclI 717 TGATCA T^GATCA
BglII 914 AGATCT A^GATCT
BpuAI 802 GAAGAC GAAGAC(2/6)
BsaBI 937 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 706 GAGACC GGTCTC(1/5)
BsiHKAI 772 GAGCAC G[AT]GC[AT]^C
BsmBI 215 GAGACG CGTCTC(1/5)
BspHI 714 TCATGA T^CATGA
BspMI 452 ACCTGC ACCTGC(4/8)
BsrFI 470 GCCGGC [GA]^CCGG[CT]
BsrI 106 ACTGG ACTGG(1/-1)
BssSI 150 CTCGTG CACGAG(-5/-1)
BstAPI 774 GCACTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 946 CGCG CG^CG
BstXI 959 CCACATTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 914 AGATCT [GA]^GATC[CT]
Cfr10I 470 GCCGGC [GA]^CCGG[CT]
DraI 54 TTTAAA TTT^AAA
DrdI 637 GACGCAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 468 TGGCCG [CT]^GGCC[GA]
EarI 1006 GAAGAG CTCTTC(1/4)
EcoT22I 974 ATGCAT ATGCA^T
FauI 593 GCGGG CCCGC(4/6)
FbaI 717 TGATCA T^GATCA
HaeII 421 GGCGCC [GA]GCGC^[CT]
HphI 295 TCACC GGTGA(8/7)
KasI 421 GGCGCC G^GCGCC
Ksp632I 1006 GAAGAG CTCTTC(1/4)
MamI 937 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 914 AGATCT [GA]^GATC[CT]
MslI 780 CATGCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 193 CAGCTG C[AC]G^C[GT]G
MvnI 946 CGCG CG^CG
NaeI 470 GCCGGC GCC^GGC
NarI 421 GGCGCC GG^CGCC
NgoMIV 470 GCCGGC G^CCGGC
NsiI 974 ATGCAT ATGCA^T
PvuII 193 CAGCTG CAG^CTG
RcaI 714 TCATGA T^CATGA
RsaI 552 GTAC GT^AC
SfoI 421 GGCGCC GGC^GCC
SmlI 632 CTCAAG C^T[CT][GA]AG
SphI 976 GCATGC GCATG^C
StuI 461 AGGCCT AGG^CCT
Tsp509I 1017 AATT ^AATT
XcmI 488 CCATGGAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 914 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 217 GACGTC GACGT^C
625 GACGTC
AlwI 282 GATCC GGATC(4/5)
399 GATCC
BmyI 541 GGGCAC G[AGT]GC[ACT]^C
772 GAGCAC
BsgI 205 CTGCAC GTGCAG(16/14)
838 CTGCAC
BsmAI 215 GAGAC GTCTC(1/5)
706 GAGAC
Bsp1286I 541 GGGCAC G[AGT]GC[ACT]^C
772 GAGCAC
BstF5I 66 CATCC GGATG(2/0)
372 CATCC
Cac8I 470 GCCGGC GC[ACGT]^[ACGT]GC
976 GCATGC
CfoI 116 GCGC GCG^C
422 GCGC
DdeI 403 CTCAG C^T[ACGT]AG
911 CTCAG
DsaI 425 CCATGG C^C[GA][CT]GG
488 CCATGG
FokI 66 CATCC GGATG(9/13)
372 CATCC
HhaI 116 GCGC GCG^C
422 GCGC
HinP1I 116 GCGC G^CGC
422 GCGC
MaeII 218 ACGT A^CGT
626 ACGT
MseI 55 TTAA T^TAA
815 TTAA
NcoI 425 CCATGG C^CATGG
488 CCATGG
NspI 779 ACATGC [GA]CATG^[CT]
976 GCATGC
PleI 378 GAGTC GAGTC(4/5)
493 GAGTC
PpuMI 254 GGGACCT [GA]G^G[AT]CC[CT]
521 AGGTCCT
PshAI 105 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
516 GACCAAGGTC
PstI 185 CTGCAG CTGCA^G
391 CTGCAG
SfcI 185 CTGCAG C^T[GA][CT]AG
391 CTGCAG
Tru9I 55 TTAA T^TAA
815 TTAA
TspRI 8 CAGTG CA[GC]TG[ACGT][ACGT]^
122 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccIII AciI AcyI AluI AlwNI
AvaII BbvI BcnI BpmI BsaHI
BsaJI BsaWI BseAI BsiYI BslI
BsmFI BspEI BssKI BstNI Cfr13I
DpnII DraII EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI HaeIII HapII HgaI
Hin1I HinfI HpaII ItaI MaeIII
MboI MboII MnlI MroI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI TseI Tsp45I
TthHB8I