(Circular) (Six-base) MAPSORT of: pFN21AE0487.seq Check: 7591 from: 1 to: 5776 pFN21AE0487 5776 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2226 2634 3586 278 Size: 53 83 186 936 690 408 952 2468 Fragments arranged by size: 2468 952 936 690 408 186 83 53 AccI GT'mk_AC Cuts at: 1937 3119 1937 Size: 1182 4594 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2816 3839 5223 5491 1632 Size: 1184 1023 1384 268 1917 Fragments arranged by size: 1917 1384 1184 1023 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 4675 5652 1203 Size: 3472 977 1327 Fragments arranged by size: 3472 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2925 1184 Size: 1741 4035 AlwNI CAG_nnn'CTG Cuts at: 2092 2945 3056 3519 5091 2092 Size: 853 111 463 1572 2777 Fragments arranged by size: 2777 1572 853 463 111 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 3085 3311 3463 5572 1493 Size: 1592 226 152 2109 1697 Fragments arranged by size: 2109 1697 1592 226 152 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 3101 1870 Size: 73 1158 4545 Fragments arranged by size: 4545 1158 73 BamHI G'GATC_C Cuts at: 3106 3473 3106 Size: 367 5409 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2426 3097 4552 618 Size: 325 217 627 639 671 1455 1842 Fragments arranged by size: 1842 1455 671 639 627 325 217 BanII G_rGCy'C Cuts at: 1391 3095 1391 Size: 1704 4072 BbeI G_GCGC'C Cuts at: 2430 2430 Size: 5776 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2814 928 Size: 971 915 3890 Fragments arranged by size: 3890 971 915 BbvCI CC'TCA_GC Cuts at: 2408 2408 Size: 5776 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2872 3668 4878 2872 Size: 796 1210 3770 Fragments arranged by size: 3770 1210 796 BclI T'GATC_A Cuts at: 1548 2722 1548 Size: 1174 4602 BfrBI ATG'CAT Cuts at: 2981 2981 Size: 5776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4387 136 Size: 107 122 71 631 605 2715 1525 Fragments arranged by size: 2715 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2919 5771 2919 Size: 2852 2924 BlpI GC'TnA_GC Cuts at: 3193 3193 Size: 5776 Bme1580I G_kGCm'C Cuts at: 1790 2550 3837 4993 1790 Size: 760 1287 1156 2573 Fragments arranged by size: 2573 1287 1156 760 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3507 4465 442 Size: 416 53 83 399 24 2090 958 1753 Fragments arranged by size: 2090 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2222 2387 2483 2648 3087 4436 1239 Size: 983 165 96 165 439 1349 2579 Fragments arranged by size: 2579 1349 983 439 165 165 96 Bpu10I CC'TnA_GC Cuts at: 2408 2408 Size: 5776 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2622 3901 4766 5064 2622 Size: 1279 865 298 3334 Fragments arranged by size: 3334 1279 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2705 4439 882 Size: 617 1206 1734 2219 Fragments arranged by size: 2219 1734 1206 617 BsaAI yAC'GTr Cuts at: 493 1204 3567 493 Size: 711 2363 2702 Fragments arranged by size: 2702 2363 711 BsaBI GATnn'nnATC Cuts at: 2946 5770 2946 Size: 2824 2952 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2115 2223 2427 2562 Size: 53 83 186 936 582 108 204 135 Cuts at: 2562 2631 3583 3965 4600 275 Size: 69 952 382 635 1451 Fragments arranged by size: 1451 952 936 635 582 382 204 186 135 108 83 69 53 BsaWI w'CCGG_w Cuts at: 2073 2235 2289 2379 4209 4607 4881 5028 5439 Size: 162 54 90 1830 398 274 147 411 Cuts at: 5439 2073 Size: 2410 Fragments arranged by size: 2410 1830 411 398 274 162 147 90 54 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3060 3090 1178 Size: 30 165 30 1657 30 3864 Fragments arranged by size: 3864 1657 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5776 BseYI C'CCAG_C Cuts at: 2324 4979 5475 2324 Size: 2655 496 2625 Fragments arranged by size: 2655 2625 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2195 2828 1163 Size: 573 459 633 4111 Fragments arranged by size: 4111 633 573 459 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2781 3837 3922 4993 2781 Size: 1056 85 1071 3564 Fragments arranged by size: 3564 1071 1056 85 BsmI GAATG_Cn' Cuts at: 3287 3380 3287 Size: 93 5683 BsmBI CGTCTCn'nnnn_ Cuts at: 2214 2214 Size: 5776 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2550 2781 3095 3837 3922 4993 1391 Size: 399 760 231 314 742 85 1071 2174 Fragments arranged by size: 2174 1071 760 742 399 314 231 85 BspEI T'CCGG_A Cuts at: 2073 2235 2289 2379 5439 2073 Size: 162 54 90 3060 2410 Fragments arranged by size: 3060 2410 162 90 54 BspHI T'CATG_A Cuts at: 2719 3665 5395 2719 Size: 946 1730 3100 Fragments arranged by size: 3100 1730 946 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2466 3131 844 Size: 1622 665 3489 Fragments arranged by size: 3489 1622 665 BsrBI CCG'CTC Cuts at: 1394 1823 3663 1394 Size: 429 1840 3507 Fragments arranged by size: 3507 1840 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4271 4445 65 Size: 1183 3023 174 1396 Fragments arranged by size: 3023 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2475 4420 4607 1700 Size: 110 665 1945 187 2869 Fragments arranged by size: 2869 1945 665 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 2155 3836 4848 2155 Size: 1681 1012 3083 Fragments arranged by size: 3083 1681 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2785 794 Size: 1991 3785 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5776 BstXI CCAn_nnnn'nTGG Cuts at: 2971 2971 Size: 5776 BstYI r'GATC_y Cuts at: 1972 2919 3106 3473 3858 3875 5327 5413 5425 Size: 947 187 367 385 17 1452 86 12 Cuts at: 5425 5771 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 947 385 367 346 187 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2430 2493 513 Size: 552 939 426 63 3796 Fragments arranged by size: 3796 939 552 426 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2328 487 Size: 683 56 1102 3935 Fragments arranged by size: 3935 1102 683 56 BtsI GCAGTG_nn' Cuts at: 2133 3292 4086 4106 2133 Size: 1159 794 20 3803 Fragments arranged by size: 3803 1159 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 2061 3080 2061 Size: 1019 4757 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2648 4783 809 Size: 507 1332 2135 1802 Fragments arranged by size: 2135 1802 1332 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2473 3223 4112 Size: 54 1425 50 275 18 643 750 889 Cuts at: 4112 5741 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 750 643 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3006 3706 1353 Size: 1653 700 3423 Fragments arranged by size: 3423 1653 700 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4363 4737 4883 387 Size: 1064 138 2774 374 146 1280 Fragments arranged by size: 2774 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2222 2387 2483 2648 2816 3087 3839 Size: 393 590 165 96 165 168 271 752 Cuts at: 3839 4436 5223 5491 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 752 597 590 393 271 268 168 165 165 96 EcoNI CCTnn'n_nnAGG Cuts at: 2307 2348 2462 2307 Size: 41 114 5621 Fragments arranged by size: 5621 114 41 EcoO109I rG'GnC_Cy Cuts at: 2260 2527 2737 2260 Size: 267 210 5299 Fragments arranged by size: 5299 267 210 EcoRI G'AATT_C Cuts at: 3085 3463 3085 Size: 378 5398 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2344 2344 Size: 5776 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 2430 4923 1678 Size: 752 2493 2531 Fragments arranged by size: 2531 2493 752 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2287 2319 4417 4449 4491 Size: 32 135 32 715 32 2098 32 42 Cuts at: 4491 4523 1373 Size: 32 2626 Fragments arranged by size: 2626 2098 715 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3120 3366 669 Size: 988 281 1182 246 3079 Fragments arranged by size: 3079 1182 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5776 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KasI G'GCGC_C Cuts at: 2426 2426 Size: 5776 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4890 5074 1305 Size: 3585 184 2007 Fragments arranged by size: 3585 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3954 Fragments arranged by size: 3954 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2789 3734 4093 4252 518 Size: 648 1623 945 359 159 2042 Fragments arranged by size: 2042 1623 945 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2200 3192 3869 5017 5262 1606 Size: 594 992 677 1148 245 2120 Fragments arranged by size: 2120 1148 992 677 594 245 NaeI GCC'GGC Cuts at: 2477 2477 Size: 5776 NarI GG'CG_CC Cuts at: 2427 2427 Size: 5776 NcoI C'CATG_G Cuts at: 513 1065 2004 2430 2493 513 Size: 552 939 426 63 3796 Fragments arranged by size: 3796 939 552 426 63 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NgoMIV G'CCGG_C Cuts at: 2475 2475 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NsiI A_TGCA'T Cuts at: 2983 2983 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 2788 2985 3134 4679 1132 Size: 97 1559 197 149 1545 2229 Fragments arranged by size: 2229 1559 1545 197 149 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5776 PfoI T'CCnGG_A Cuts at: 2334 2890 2334 Size: 556 5220 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2260 2527 2260 Size: 267 5509 PshAI GACnn'nnGTC Cuts at: 1613 2114 2525 1613 Size: 501 411 4864 Fragments arranged by size: 4864 501 411 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PstI C_TGCA'G Cuts at: 830 2194 2400 3128 830 Size: 1364 206 728 3478 Fragments arranged by size: 3478 1364 728 206 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 PvuII CAG'CTG Cuts at: 2200 2200 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 4024 5736 4024 Size: 1712 4064 SfcI C'TryA_G Cuts at: 826 1045 2190 2396 3124 4259 4940 5131 826 Size: 219 1145 206 728 1135 681 191 1471 Fragments arranged by size: 1471 1145 1135 728 681 219 206 191 SfoI GGC'GCC Cuts at: 2428 2428 Size: 5776 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 3103 1872 Size: 1231 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2637 3880 4781 5043 820 Size: 197 926 694 1243 901 262 1553 Fragments arranged by size: 1553 1243 926 901 694 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 2985 3134 1132 Size: 1853 149 3774 Fragments arranged by size: 3774 1853 149 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 2468 1517 Size: 951 4825 StyI C'CwwG_G Cuts at: 513 1065 2004 2430 2493 2523 3215 513 Size: 552 939 426 63 30 692 3074 Fragments arranged by size: 3074 939 692 552 426 63 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3803 3820 3973 4158 4603 325 Size: 1255 2223 17 153 185 445 1498 Fragments arranged by size: 2223 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4022 5734 96 Size: 274 80 33 51 1443 2045 1712 138 Fragments arranged by size: 2045 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5736 5768 5736 Size: 32 5744 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2500 2011 Size: 489 5287 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 3101 1870 Size: 1231 4545 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2224 2632 3584 276 Size: 53 83 186 936 690 408 952 2468 Fragments arranged by size: 2468 952 936 690 408 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI