(Circular) (Six-base) MAPSORT of: pFN21AE0507.seq Check: 9297 from: 1 to: 5482 pFN21AE0507 5482 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2310 2310 Size: 5482 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3292 278 Size: 53 83 186 936 1756 2468 Fragments arranged by size: 2468 1756 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2825 1937 Size: 888 4594 Acc65I G'GTAC_C Cuts at: 2803 2803 Size: 5482 AclI AA'CG_TT Cuts at: 1563 3609 3982 1563 Size: 2046 373 3063 Fragments arranged by size: 3063 2046 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3545 4929 5197 1632 Size: 1913 1384 268 1917 Fragments arranged by size: 1917 1913 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5285 AflIII A'CryG_T Cuts at: 1203 4381 5358 1203 Size: 3178 977 1327 Fragments arranged by size: 3178 1327 977 AgeI A'CCGG_T Cuts at: 4313 4313 Size: 5482 AhdI GACnn_n'nnGTC Cuts at: 4211 4211 Size: 5482 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5482 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2634 1184 Size: 1450 4032 AlwNI CAG_nnn'CTG Cuts at: 2428 2472 3225 4797 2428 Size: 44 753 1572 3113 Fragments arranged by size: 3113 1572 753 44 ApaI G_GGCC'C Cuts at: 2093 2191 2618 2743 2093 Size: 98 427 125 4832 Fragments arranged by size: 4832 427 125 98 ApaLI G'TGCA_C Cuts at: 3539 4695 3539 Size: 1156 4326 ApoI r'AATT_y Cuts at: 1493 2791 3017 3169 5278 1493 Size: 1298 226 152 2109 1697 Fragments arranged by size: 2109 1697 1298 226 152 AseI AT'TA_AT Cuts at: 160 1032 4036 160 Size: 872 3004 1606 Fragments arranged by size: 3004 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5482 AvaI C'yCGr_G Cuts at: 1870 1943 2249 2355 2807 1870 Size: 73 306 106 452 4545 Fragments arranged by size: 4545 452 306 106 73 AvrII C'CTAG_G Cuts at: 2632 2632 Size: 5482 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2378 2411 2378 Size: 33 5449 BamHI G'GATC_C Cuts at: 2812 3179 2812 Size: 367 5115 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2803 4258 618 Size: 325 217 627 1016 1455 1842 Fragments arranged by size: 1842 1455 1016 627 325 217 BanII G_rGCy'C Cuts at: 1391 2093 2191 2618 2651 2743 2801 1391 Size: 702 98 427 33 92 58 4072 Fragments arranged by size: 4072 702 427 98 92 58 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4511 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2240 2274 3673 3707 1903 Size: 34 303 34 1399 34 3678 Fragments arranged by size: 3678 1399 303 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3374 4584 3374 Size: 1210 4272 BclI T'GATC_A Cuts at: 1548 1548 Size: 5482 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2333 2550 4093 Size: 107 122 71 631 605 661 217 1543 Cuts at: 4093 136 Size: 1525 Fragments arranged by size: 1543 1525 661 631 605 217 122 107 71 BglII A'GATC_T Cuts at: 5477 5477 Size: 5482 BlpI GC'TnA_GC Cuts at: 2899 2899 Size: 5482 Bme1580I G_kGCm'C Cuts at: 1790 2093 2191 2250 2618 2743 3543 4699 1790 Size: 303 98 59 368 125 800 1156 2573 Fragments arranged by size: 2573 1156 800 368 303 125 98 59 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5482 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2106 3213 4171 Size: 416 53 83 399 24 689 1107 958 Cuts at: 4171 442 Size: 1753 Fragments arranged by size: 1753 1107 958 689 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5482 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2307 2531 4142 1239 Size: 1068 224 1611 2579 Fragments arranged by size: 2579 1611 1068 224 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2129 3607 4472 4770 2129 Size: 1478 865 298 2841 Fragments arranged by size: 2841 1478 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2657 4145 882 Size: 617 1158 1488 2219 Fragments arranged by size: 2219 1488 1158 617 BsaAI yAC'GTr Cuts at: 493 1204 3273 493 Size: 711 2069 2702 Fragments arranged by size: 2702 2069 711 BsaBI GATnn'nnATC Cuts at: 5476 5476 Size: 5482 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3289 3671 4306 275 Size: 53 83 186 936 1756 382 635 1451 Fragments arranged by size: 1756 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3915 4313 4587 4734 5145 3915 Size: 398 274 147 411 4252 Fragments arranged by size: 4252 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2390 2420 2513 2543 1178 Size: 30 165 30 987 30 93 30 4117 Fragments arranged by size: 4117 987 165 93 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2035 2267 2618 1186 Size: 849 232 351 4050 Fragments arranged by size: 4050 849 351 232 BseYI C'CCAG_C Cuts at: 2053 4685 5181 2053 Size: 2632 496 2354 Fragments arranged by size: 2632 2354 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2203 1163 Size: 573 467 4442 Fragments arranged by size: 4442 573 467 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2932 3693 4721 1540 Size: 275 186 931 761 1028 2301 Fragments arranged by size: 2301 1028 931 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3543 3628 4699 3543 Size: 85 1071 4326 Fragments arranged by size: 4326 1071 85 BsmI GAATG_Cn' Cuts at: 2993 3086 2993 Size: 93 5389 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2191 2250 2618 2651 2743 2801 Size: 399 303 98 59 368 33 92 58 Cuts at: 2801 3543 3628 4699 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 368 303 98 92 85 59 58 33 BspEI T'CCGG_A Cuts at: 5145 5145 Size: 5482 BspHI T'CATG_A Cuts at: 3371 5101 3371 Size: 1730 3752 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2163 2310 2565 2837 844 Size: 1319 147 255 272 3489 Fragments arranged by size: 3489 1319 272 255 147 BsrBI CCG'CTC Cuts at: 1394 1823 2445 3369 1394 Size: 429 622 924 3507 Fragments arranged by size: 3507 924 622 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3977 4151 65 Size: 1183 2729 174 1396 Fragments arranged by size: 2729 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2320 4126 4313 1700 Size: 110 510 1806 187 2869 Fragments arranged by size: 2869 1806 510 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5482 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5482 BssSI C'ACGA_G Cuts at: 3542 4554 3542 Size: 1012 4470 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5482 BstBI TT'CG_AA Cuts at: 4286 4286 Size: 5482 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5482 BstYI r'GATC_y Cuts at: 1972 2812 3179 3564 3581 5033 5119 5131 5477 Size: 840 367 385 17 1452 86 12 346 Cuts at: 5477 1972 Size: 1977 Fragments arranged by size: 1977 1452 840 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4930 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4743 Fragments arranged by size: 4743 683 56 BtsI GCAGTG_nn' Cuts at: 2521 2729 2998 3792 3812 2521 Size: 208 269 794 20 4191 Fragments arranged by size: 4191 794 269 208 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5482 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5447 DraI TTT'AAA Cuts at: 2786 2786 Size: 5482 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4489 809 Size: 507 3173 1802 Fragments arranged by size: 3173 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2122 2232 2518 Size: 54 1425 50 275 18 292 110 286 Cuts at: 2518 2698 2929 3818 5447 8 Size: 180 231 889 1629 43 Fragments arranged by size: 1629 1425 889 292 286 275 231 180 110 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2929 1537 Size: 275 1117 4090 Fragments arranged by size: 4090 1117 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2729 3412 1353 Size: 1376 683 3423 Fragments arranged by size: 3423 1376 683 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4069 4443 4589 387 Size: 1064 138 2480 374 146 1280 Fragments arranged by size: 2480 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2307 2531 3545 4142 4929 5197 1239 Size: 393 675 224 1014 597 787 268 1524 Fragments arranged by size: 1524 1014 787 675 597 393 268 224 EcoNI CCTnn'n_nnAGG Cuts at: 2531 2636 2531 Size: 105 5377 EcoO109I rG'GnC_Cy Cuts at: 2089 2097 2188 2614 2615 2739 2089 Size: 8 91 426 1 124 4832 Fragments arranged by size: 4832 426 124 91 8 1 EcoRI G'AATT_C Cuts at: 2791 3169 2791 Size: 378 5104 FspI TGC'GCA Cuts at: 2078 3988 2078 Size: 1910 3572 HaeII r_GCGC'y Cuts at: 1678 4629 1678 Size: 2951 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4123 4155 4197 4229 1373 Size: 32 135 32 2551 32 42 32 2626 Fragments arranged by size: 2626 2551 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2826 3072 669 Size: 988 281 888 246 3079 Fragments arranged by size: 3079 988 888 281 246 HindIII A'AGCT_T Cuts at: 748 2362 748 Size: 1614 3868 HpaI GTT'AAC Cuts at: 3072 3072 Size: 5482 KpnI G_GTAC'C Cuts at: 2807 2807 Size: 5482 MfeI C'AATT_G Cuts at: 3081 3081 Size: 5482 MluI A'CGCG_T Cuts at: 5358 5358 Size: 5482 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2376 4596 4780 1305 Size: 1071 2220 184 2007 Fragments arranged by size: 2220 2007 1071 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2520 2700 10 Size: 54 1425 343 688 180 2792 Fragments arranged by size: 2792 1425 688 343 180 54 MslI CAynn'nnrTG Cuts at: 518 1166 3440 3799 3958 518 Size: 648 2274 359 159 2042 Fragments arranged by size: 2274 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2057 2472 2898 3575 4723 4968 1606 Size: 451 415 426 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 451 426 415 245 NaeI GCC'GGC Cuts at: 2322 2322 Size: 5482 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4930 NdeI CA'TA_TG Cuts at: 387 387 Size: 5482 NgoMIV G'CCGG_C Cuts at: 2320 2320 Size: 5482 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5482 NotI GC'GGCC_GC Cuts at: 2929 2929 Size: 5482 NruI TCG'CGA Cuts at: 2178 2178 Size: 5482 NspI r_CATG'y Cuts at: 1132 1229 2377 2840 4385 1132 Size: 97 1148 463 1545 2229 Fragments arranged by size: 2229 1545 1148 463 97 PasI CC'CwG_GG Cuts at: 1348 2093 2743 1348 Size: 745 650 4087 Fragments arranged by size: 4087 745 650 PciI A'CATG_T Cuts at: 4381 4381 Size: 5482 PflMI CCAn_nnn'nTGG Cuts at: 1108 2428 1108 Size: 1320 4162 PfoI T'CCnGG_A Cuts at: 2450 2450 Size: 5482 PmeI GTTT'AAAC Cuts at: 2786 2786 Size: 5482 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3551 3583 5064 5096 3551 Size: 32 1481 32 3937 Fragments arranged by size: 3937 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2097 2097 Size: 5482 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5482 PsiI TTA'TAA Cuts at: 3052 3052 Size: 5482 PspOMI G'GGCC_C Cuts at: 2089 2187 2614 2739 2089 Size: 98 427 125 4832 Fragments arranged by size: 4832 427 125 98 PstI C_TGCA'G Cuts at: 830 2314 2584 2613 2834 830 Size: 1484 270 29 221 3478 Fragments arranged by size: 3478 1484 270 221 29 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5482 SalI G'TCGA_C Cuts at: 1936 2824 1936 Size: 888 4594 SanDI GG'GwC_CC Cuts at: 2097 2097 Size: 5482 SbfI CC_TGCA'GG Cuts at: 2834 2834 Size: 5482 ScaI AGT'ACT Cuts at: 3730 5442 3730 Size: 1712 3770 SfcI C'TryA_G Cuts at: 826 1045 2310 2580 2609 2830 3965 4646 4837 Size: 219 1265 270 29 221 1135 681 191 Cuts at: 4837 826 Size: 1471 Fragments arranged by size: 1471 1265 1135 681 270 221 219 191 29 SfiI GGCCn_nnn'nGGCC Cuts at: 2333 2333 Size: 5482 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5482 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5482 SmaI CCC'GGG Cuts at: 1872 2357 2809 1872 Size: 485 452 4545 Fragments arranged by size: 4545 485 452 SmlI C'TyrA_G Cuts at: 820 1017 1943 2144 3586 4487 4749 820 Size: 197 926 201 1442 901 262 1553 Fragments arranged by size: 1553 1442 926 901 262 201 197 SnaBI TAC'GTA Cuts at: 493 3273 493 Size: 2780 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5482 SphI G_CATG'C Cuts at: 1132 2840 1132 Size: 1708 3774 SspI AAT'ATT Cuts at: 5 52 3406 5 Size: 47 3354 2081 Fragments arranged by size: 3354 2081 47 StuI AGG'CCT Cuts at: 1517 2336 1517 Size: 819 4663 StyI C'CwwG_G Cuts at: 513 1065 2532 2632 2921 513 Size: 552 1467 100 289 3074 Fragments arranged by size: 3074 1467 552 289 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2329 3509 3526 3679 3864 4309 325 Size: 1255 749 1180 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1180 749 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3728 5440 96 Size: 274 80 33 51 1443 1751 1712 138 Fragments arranged by size: 1751 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2513 2545 5442 5474 2513 Size: 32 2897 32 2521 Fragments arranged by size: 2897 2521 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5482 XbaI T'CTAG_A Cuts at: 2818 2818 Size: 5482 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5482 XmaI C'CCGG_G Cuts at: 1870 2355 2807 1870 Size: 485 452 4545 Fragments arranged by size: 4545 485 452 XmnI GAAnn'nnTTC Cuts at: 3611 3611 Size: 5482 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3290 276 Size: 53 83 186 936 1756 2468 Fragments arranged by size: 2468 1756 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfoI SrfI SwaI XcmI