Restriction map for
pFN21AE0507
(Linear) (Six-base) MAPSORT of: KIEE0507.seq Check: 5476 from: 1 to: 777
KIEE0507.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 305 777
Size: 305 472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 629 777
Size: 629 148
AlwNI CAG_nnn'CTG
Cuts at: 0 423 467 777
Size: 423 44 310
Fragments arranged by size:
423 310 44
ApaI G_GGCC'C
Cuts at: 0 88 186 613 738 777
Size: 88 98 427 125 39
Fragments arranged by size:
427 125 98 88 39
AvaI C'yCGr_G
Cuts at: 0 244 350 777
Size: 244 106 427
Fragments arranged by size:
427 244 106
AvrII C'CTAG_G
Cuts at: 0 627 777
Size: 627 150
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 373 406 777
Size: 373 33 371
Fragments arranged by size:
373 371 33
BanII G_rGCy'C
Cuts at: 0 88 186 613 646 738 777
Size: 88 98 427 33 92 39
Fragments arranged by size:
427 98 92 88 39 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 235 269 777
Size: 235 34 508
Fragments arranged by size:
508 235 34
BglI GCCn_nnn'nGGC
Cuts at: 0 328 545 777
Size: 328 217 232
Fragments arranged by size:
328 232 217
Bme1580I G_kGCm'C
Cuts at: 0 88 186 245 613 738 777
Size: 88 98 59 368 125 39
Fragments arranged by size:
368 125 98 88 59 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 101 777
Size: 101 676
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 526 777
Size: 302 224 251
Fragments arranged by size:
302 251 224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 777
Size: 124 653
BsaI GGTCTCn'nnnn_
Cuts at: 0 652 777
Size: 652 125
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 385 415 508 538 777
Size: 385 30 93 30 239
Fragments arranged by size:
385 239 93 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 30 262 613 777
Size: 30 232 351 164
Fragments arranged by size:
351 232 164 30
BseYI C'CCAG_C
Cuts at: 0 48 777
Size: 48 729
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 198 777
Size: 198 579
Bsp1286I G_dGCh'C
Cuts at: 0 88 186 245 613 646 738 777
Size: 88 98 59 368 33 92 39
Fragments arranged by size:
368 98 92 88 59 39 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 158 305 560 777
Size: 158 147 255 217
Fragments arranged by size:
255 217 158 147
BsrBI CCG'CTC
Cuts at: 0 440 777
Size: 440 337
BsrFI r'CCGG_y
Cuts at: 0 315 777
Size: 315 462
BtsI GCAGTG_nn'
Cuts at: 0 516 724 777
Size: 516 208 53
Fragments arranged by size:
516 208 53
EaeI y'GGCC_r
Cuts at: 0 117 227 513 693 777
Size: 117 110 286 180 84
Fragments arranged by size:
286 180 117 110 84
EarI CTCTTCn'nnn_
Cuts at: 0 724 777
Size: 724 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 526 777
Size: 302 224 251
Fragments arranged by size:
302 251 224
EcoNI CCTnn'n_nnAGG
Cuts at: 0 526 631 777
Size: 526 105 146
Fragments arranged by size:
526 146 105
EcoO109I rG'GnC_Cy
Cuts at: 0 84 92 183 609 610 734 777
Size: 84 8 91 426 1 124 43
Fragments arranged by size:
426 124 91 84 43 8 1
FspI TGC'GCA
Cuts at: 0 73 777
Size: 73 704
HindIII A'AGCT_T
Cuts at: 0 357 777
Size: 357 420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 777
Size: 371 406
MscI TGG'CCA
Cuts at: 0 515 695 777
Size: 515 180 82
Fragments arranged by size:
515 180 82
MspA1I CmG'CkG
Cuts at: 0 52 467 777
Size: 52 415 310
Fragments arranged by size:
415 310 52
NaeI GCC'GGC
Cuts at: 0 317 777
Size: 317 460
NgoMIV G'CCGG_C
Cuts at: 0 315 777
Size: 315 462
NruI TCG'CGA
Cuts at: 0 173 777
Size: 173 604
NspI r_CATG'y
Cuts at: 0 372 777
Size: 372 405
PasI CC'CwG_GG
Cuts at: 0 88 738 777
Size: 88 650 39
Fragments arranged by size:
650 88 39
PflMI CCAn_nnn'nTGG
Cuts at: 0 423 777
Size: 423 354
PfoI T'CCnGG_A
Cuts at: 0 445 777
Size: 445 332
PpuMI rG'GwC_Cy
Cuts at: 0 92 777
Size: 92 685
PspOMI G'GGCC_C
Cuts at: 0 84 182 609 734 777
Size: 84 98 427 125 43
Fragments arranged by size:
427 125 98 84 43
PstI C_TGCA'G
Cuts at: 0 309 579 608 777
Size: 309 270 29 169
Fragments arranged by size:
309 270 169 29
SanDI GG'GwC_CC
Cuts at: 0 92 777
Size: 92 685
SfcI C'TryA_G
Cuts at: 0 305 575 604 777
Size: 305 270 29 173
Fragments arranged by size:
305 270 173 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 328 777
Size: 328 449
SmaI CCC'GGG
Cuts at: 0 352 777
Size: 352 425
SmlI C'TyrA_G
Cuts at: 0 139 777
Size: 139 638
StuI AGG'CCT
Cuts at: 0 331 777
Size: 331 446
StyI C'CwwG_G
Cuts at: 0 527 627 777
Size: 527 100 150
Fragments arranged by size:
527 150 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 324 777
Size: 324 453
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 508 540 777
Size: 508 32 237
Fragments arranged by size:
508 237 32
XmaI C'CCGG_G
Cuts at: 0 350 777
Size: 350 427
Enzymes that do cut:
AarI AlfI AlwNI ApaI AvaI AvrII BaeI BanII
BcgI BglI Bme1580I BmrI BpmI BpuEI BsaI BsaXI
BseRI BseYI BsgI Bsp1286I BspMI BsrBI BsrFI BtsI
EaeI EarI Eco57MI EcoNI EcoO109I FspI HindIII MmeI
MscI MspA1I NaeI NgoMIV NruI NspI PasI PflMI
PfoI PpuMI PspOMI PstI SanDI SfcI SfiI SmaI
SmlI StuI StyI TaqII TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI ApoI AscI AseI
AsiSI BamHI BanI BbeI BbsI BbvCI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspAI HaeII Hin4I HincII HpaI
KasI KpnI MfeI MluI MslI NarI NcoI NdeI
NheI NotI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XcmI XhoI XmnI ZraI