Restriction map for
pFN21AE0152
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# NO CUT ENZYMES ( 202 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BfaI BfrI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FokI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MaeII MaeIII MfeI
MluI MluNI MroI MscI MslI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI Sau96I SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TaqI Tsp45I TspRI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AlwNI 52 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 325 CCCGAG C^[CT]CG[GA]G
BclI 59 TGATCA T^GATCA
BcnI 80 CCCGG CC^[GC]GG
BglII 93 AGATCT A^GATCT
BsaBI 25 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 185 GAGACC GGTCTC(1/5)
BsaJI 383 CCCAGG C^C[ACGT][ACGT]GG
BseRI 137 CTCCTC GAGGAG(10/8)
BsiYI 378 CCAAGCCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 378 CCAAGCCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 185 GAGAC GTCTC(1/5)
BsoBI 325 CCCGAG C^[CT]CG[GA]G
BstAPI 294 GCAGAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 384 CCAGG CC^[AT]GG
BstYI 93 AGATCT [GA]^GATC[CT]
EaeI 2 TGGCCG [CT]^GGCC[GA]
EarI 307 GAAGAG CTCTTC(1/4)
EcoRII 384 CCAGG ^CC[AT]GG
FbaI 59 TGATCA T^GATCA
HapII 81 CCGG C^CGG
HpaII 81 CCGG C^CGG
Ksp632I 307 GAAGAG CTCTTC(1/4)
MamI 25 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 93 AGATCT [GA]^GATC[CT]
MseI 253 TTAA T^TAA
MspI 81 CCGG C^CGG
MvaI 384 CCAGG CC^[AT]GG
NciI 80 CCCGG CC^[GC]GG
NlaIV 83 GGAACC GG[ACGT]^[ACGT]CC
PleI 388 GAGTC GAGTC(4/5)
SapI 307 GAAGAGC GCTCTTC(1/4)
SmlI 286 CTTGAG C^T[CT][GA]AG
StuI 46 AGGCCT AGG^CCT
TfiI 117 GAATC G^A[AT]TC
Tru9I 253 TTAA T^TAA
XhoII 93 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AluI 248 AGCT AG^CT
278 AGCT
BbvI 261 GCAGC GCAGC(8/12)
276 GCAGC
BssKI 80 CCCGG ^CC[ACGT]GG
384 CCAGG
DpnII 60 GATC ^GATC
94 GATC
Fnu4HI 261 GCAGC GC^[ACGT]GC
276 GCAGC
HinfI 117 GAATC G^A[ACGT]TC
388 GAGTC
HphI 165 GGTGA GGTGA(8/7)
339 GGTGA
ItaI 261 GCAGC GC^[ACGT]GC
276 GCAGC
MboI 60 GATC ^GATC
94 GATC
MwoI 294 GCAGAAATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
303 GCAAGAAGAGC
NdeII 60 GATC ^GATC
94 GATC
Sau3AI 60 GATC ^GATC
94 GATC
ScrFI 80 CCCGG CC^[ACGT]GG
384 CCAGG
TseI 261 GCAGC G^C[AT]GC
276 GCAGC
Tsp509I 155 AATT ^AATT
299 AATT
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# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
#########################################
DdeI HaeIII MboII MnlI