################################## # NO CUT ENZYMES ( 124 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AgeI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvaI AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BseRI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrGI BssHII BssSI Bst1107I BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV FseI HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MseI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcyI 162 GACGCC G[GA]^CG[CT]C AhdI 259 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 484 GTGCAC G^TGCAC ApaI 1038 GGGCCC GGGCC^C ApaLI 484 GTGCAC G^TGCAC AspEI 259 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 785 TGCGCA TGC^GCA BamHI 25 GGATCC G^GATCC BsaAI 856 TACGTG [CT]AC^GT[GA] BsaHI 162 GACGCC G[GA]^CG[CT]C BsaI 257 GAGACC GGTCTC(1/5) BsiEI 465 CGACCG CG[GA][CT]^CG BspLU11I 425 ACATGT A^CATGT BsrBI 846 CCGCTC CCGCTC(-3/-3) BsrDI 147 GCAATG GCAATG(2/0) BsrFI 403 ACCGGC [GA]^CCGG[CT] BstAPI 744 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1054 TTCGAA TT^CGAA BstEII 708 GGTCACC G^GT[ACGT]ACC BstPI 708 GGTCACC G^GT[ACGT]ACC BstYI 25 GGATCC [GA]^GATC[CT] Cfr10I 403 ACCGGC [GA]^CCGG[CT] DraIII 613 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 259 GACCACAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 412 CTCTTC CTCTTC(1/4) EcoNI 738 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 708 GGTCACC G^GT[ACGT]ACC EcoT22I 295 ATGCAT ATGCA^T FspI 785 TGCGCA TGC^GCA HgaI 162 GACGC GACGC(5/10) Hin1I 162 GACGCC G[GA]^CG[CT]C HincII 841 GTCAAC GT[CT]^[GA]AC HindII 841 GTCAAC GT[CT]^[GA]AC HinfI 70 GACTC G^A[ACGT]TC Ksp632I 412 CTCTTC CTCTTC(1/4) MaeIII 709 GTCAC ^GT[ACGT]AC MflI 25 GGATCC [GA]^GATC[CT] NsiI 295 ATGCAT ATGCA^T NspV 1054 TTCGAA TT^CGAA PleI 70 GACTC GAGTC(4/5) PpuMI 271 GGGACCC [GA]G^G[AT]CC[CT] PstI 628 CTGCAG CTGCA^G PvuII 631 CAGCTG CAG^CTG SacI 1074 GAGCTC GAGCT^C SfcI 628 CTGCAG C^T[GA][CT]AG SfuI 1054 TTCGAA TT^CGAA SphI 391 GCATGC GCATG^C StuI 647 AGGCCT AGG^CCT Tsp45I 709 GTCAC ^GT[GC]AC XhoII 25 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 349 GTCTAC GT^[AC][GT]AC 853 GTCTAC AfaI 429 GTAC GT^AC 636 GTAC AflIII 284 ACGTGT A^C[GA][CT]GT 425 ACATGT AlwI 26 GATCC GGATC(4/5) 25 GGATC AlwNI 62 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG 745 CAGGGCCTG AspHI 484 GTGCAC G[AT]GC[AT]^C 1074 GAGCTC AvaII 272 GGACC G^G[AT]CC 330 GGTCC BclI 116 TGATCA T^GATCA 725 TGATCA BpmI 72 CTCCAG CTGGAG(16/14) 169 CTGGAG BsgI 862 CTGCAC GTGCAG(16/14) 601 GTGCAG BsiHKAI 484 GTGCAC G[AT]GC[AT]^C 1074 GAGCTC BsmBI 397 CGTCTC CGTCTC(1/5) 160 GAGACG BsrI 168 ACTGG ACTGG(1/-1) 371 ACTGG FbaI 116 TGATCA T^GATCA 725 TGATCA HaeII 460 AGCGCC [GA]GCGC^[CT] 764 AGCGCC MspA1I 577 CCGCTG C[AC]G^C[GT]G 631 CAGCTG NcoI 569 CCATGG C^CATGG 876 CCATGG PflMI 612 CCACACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 657 CCATGCCCTGG RsaI 429 GTAC GT^AC 636 GTAC TaqI 959 TCGA T^CGA 1055 TCGA TthHB8I 959 TCGA T^CGA 1055 TCGA Van91I 612 CCACACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 657 CCATGCCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AluI BalI BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MboI MboII MluNI MnlI MscI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TseI TspRI XcmI