(Circular) (Six-base) MAPSORT of: pFN21AB8973.seq Check: 6605 from: 1 to: 5806 pFN21AB8973 5806 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2465 2726 2465 Size: 261 5545 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3616 278 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2355 2859 3149 1937 Size: 418 504 290 4594 Fragments arranged by size: 4594 504 418 290 Acc65I G'GTAC_C Cuts at: 3127 3127 Size: 5806 AclI AA'CG_TT Cuts at: 1563 3933 4306 1563 Size: 2370 373 3063 Fragments arranged by size: 3063 2370 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3869 5253 5521 1632 Size: 2237 1384 268 1917 Fragments arranged by size: 2237 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5609 AflIII A'CryG_T Cuts at: 1203 2289 2430 4705 5682 1203 Size: 1086 141 2275 977 1327 Fragments arranged by size: 2275 1327 1086 977 141 AgeI A'CCGG_T Cuts at: 4637 4637 Size: 5806 AhdI GACnn_n'nnGTC Cuts at: 2269 4535 2269 Size: 2266 3540 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5806 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5806 AlwNI CAG_nnn'CTG Cuts at: 2072 2755 3549 5121 2072 Size: 683 794 1572 2757 Fragments arranged by size: 2757 1572 794 683 ApaI G_GGCC'C Cuts at: 3047 3047 Size: 5806 ApaLI G'TGCA_C Cuts at: 2489 3863 5019 2489 Size: 1374 1156 3276 Fragments arranged by size: 3276 1374 1156 ApoI r'AATT_y Cuts at: 1493 3115 3341 3493 5602 1493 Size: 1622 226 152 2109 1697 Fragments arranged by size: 2109 1697 1622 226 152 AseI AT'TA_AT Cuts at: 160 1032 4360 160 Size: 872 3328 1606 Fragments arranged by size: 3328 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 AvaI C'yCGr_G Cuts at: 1870 1943 3131 1870 Size: 73 1188 4545 Fragments arranged by size: 4545 1188 73 BamHI G'GATC_C Cuts at: 2030 3136 3503 2030 Size: 1106 367 4333 Fragments arranged by size: 4333 1106 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3127 4582 618 Size: 325 217 627 1340 1455 1842 Fragments arranged by size: 1842 1455 1340 627 325 217 BanII G_rGCy'C Cuts at: 1391 2597 3047 3083 3125 1391 Size: 1206 450 36 42 4072 Fragments arranged by size: 4072 1206 450 42 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4835 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3997 4031 1903 Size: 34 2060 34 3678 Fragments arranged by size: 3678 2060 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3698 4908 3698 Size: 1210 4596 BclI T'GATC_A Cuts at: 1548 2121 2730 1548 Size: 573 609 4624 Fragments arranged by size: 4624 609 573 BfrBI ATG'CAT Cuts at: 2302 2302 Size: 5806 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4417 136 Size: 107 122 71 631 605 2745 1525 Fragments arranged by size: 2745 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5801 5801 Size: 5806 BlpI GC'TnA_GC Cuts at: 3223 3223 Size: 5806 Bme1580I G_kGCm'C Cuts at: 1790 2493 2838 2962 3047 3867 5023 1790 Size: 703 345 124 85 820 1156 2573 Fragments arranged by size: 2573 1156 820 703 345 124 85 BmgBI CAC'GTC Cuts at: 1318 2845 1318 Size: 1527 4279 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3537 4495 442 Size: 416 53 83 399 24 2120 958 1753 Fragments arranged by size: 2120 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5806 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2671 2671 Size: 5806 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2062 2195 4466 1239 Size: 823 133 2271 2579 Fragments arranged by size: 2579 2271 823 133 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2492 2759 3023 3931 4796 5094 2492 Size: 267 264 908 865 298 3204 Fragments arranged by size: 3204 908 865 298 267 264 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2256 4469 882 Size: 617 757 2213 2219 Fragments arranged by size: 2219 2213 757 617 BsaAI yAC'GTr Cuts at: 493 1204 2863 3597 493 Size: 711 1659 734 2702 Fragments arranged by size: 2702 1659 734 711 BsaBI GATnn'nnATC Cuts at: 5800 5800 Size: 5806 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2168 3613 3995 4630 Size: 53 83 186 936 635 1445 382 635 Cuts at: 4630 275 Size: 1451 Fragments arranged by size: 1451 1445 936 635 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4239 4637 4911 5058 5469 4239 Size: 398 274 147 411 4576 Fragments arranged by size: 4576 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5581 Fragments arranged by size: 5581 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5806 BseYI C'CCAG_C Cuts at: 2901 3053 5009 5505 2901 Size: 152 1956 496 3202 Fragments arranged by size: 3202 1956 496 152 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2627 2852 1163 Size: 573 891 225 4117 Fragments arranged by size: 4117 891 573 225 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2473 3256 4017 5045 1540 Size: 275 186 472 783 761 1028 2301 Fragments arranged by size: 2301 1028 783 761 472 275 186 BsiHKAI G_wGCw'C Cuts at: 2493 3083 3867 3952 5023 2493 Size: 590 784 85 1071 3276 Fragments arranged by size: 3276 1071 784 590 85 BsmI GAATG_Cn' Cuts at: 3317 3410 3317 Size: 93 5713 BsmBI CGTCTCn'nnnn_ Cuts at: 2159 2408 2159 Size: 249 5557 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2493 2597 2838 2962 3047 3083 3125 Size: 399 703 104 241 124 85 36 42 Cuts at: 3125 3867 3952 5023 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 703 399 241 124 104 85 85 42 36 BspEI T'CCGG_A Cuts at: 5469 5469 Size: 5806 BspHI T'CATG_A Cuts at: 3695 5425 3695 Size: 1730 4076 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2465 2726 2733 3161 844 Size: 1621 261 7 428 3489 Fragments arranged by size: 3489 1621 428 261 7 BsrBI CCG'CTC Cuts at: 1394 1823 2853 3693 1394 Size: 429 1030 840 3507 Fragments arranged by size: 3507 1030 840 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2159 4301 4475 65 Size: 1183 911 2142 174 1396 Fragments arranged by size: 2142 1396 1183 911 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2408 4450 4637 1700 Size: 110 598 2042 187 2869 Fragments arranged by size: 2869 2042 598 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5806 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5806 BssSI C'ACGA_G Cuts at: 3866 4878 3866 Size: 1012 4794 BstAPI GCAn_nnn'nTGC Cuts at: 794 2755 794 Size: 1961 3845 BstBI TT'CG_AA Cuts at: 3060 4610 3060 Size: 1550 4256 BstEII G'GTnAC_C Cuts at: 1184 2713 1184 Size: 1529 4277 BstXI CCAn_nnnn'nTGG Cuts at: 2350 2366 2809 2821 2350 Size: 16 443 12 5335 Fragments arranged by size: 5335 443 16 12 BstYI r'GATC_y Cuts at: 1972 2030 3136 3503 3888 3905 5357 5443 5455 Size: 58 1106 367 385 17 1452 86 12 Cuts at: 5455 5801 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1106 385 367 346 86 58 17 12 BtgI C'CryG_G Cuts at: 513 1065 2142 2310 2574 2709 2881 513 Size: 552 1077 168 264 135 172 3438 Fragments arranged by size: 3438 1077 552 264 172 168 135 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5067 Fragments arranged by size: 5067 683 56 BtsI GCAGTG_nn' Cuts at: 2688 2793 3322 4116 4136 2688 Size: 105 529 794 20 4358 Fragments arranged by size: 4358 794 529 105 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5806 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5771 DraI TTT'AAA Cuts at: 3110 3110 Size: 5806 DraIII CAC_nnn'GTG Cuts at: 2623 2623 Size: 5806 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4813 809 Size: 507 3497 1802 Fragments arranged by size: 3497 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2139 2158 2283 Size: 54 1425 50 275 18 309 19 125 Cuts at: 2283 2307 2313 2378 2481 2571 2811 3253 4142 Size: 24 6 65 103 90 240 442 889 Cuts at: 4142 5771 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 442 309 275 240 125 103 90 65 54 50 43 24 19 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3253 1537 Size: 275 1441 4090 Fragments arranged by size: 4090 1441 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2423 3736 1353 Size: 1070 1313 3423 Fragments arranged by size: 3423 1313 1070 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2990 4393 4767 4913 387 Size: 1064 138 1401 1403 374 146 1280 Fragments arranged by size: 1403 1401 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3081 3081 Size: 5806 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2062 2195 3869 4466 5253 5521 1239 Size: 393 430 133 1674 597 787 268 1524 Fragments arranged by size: 1674 1524 787 597 430 393 268 133 EcoNI CCTnn'n_nnAGG Cuts at: 2747 2747 Size: 5806 EcoO109I rG'GnC_Cy Cuts at: 2277 2520 2752 2886 3043 2277 Size: 243 232 134 157 5040 Fragments arranged by size: 5040 243 232 157 134 EcoRI G'AATT_C Cuts at: 3115 3493 3115 Size: 378 5428 FspI TGC'GCA Cuts at: 2792 4312 2792 Size: 1520 4286 FspAI rTGC'GCAy Cuts at: 2792 2792 Size: 5806 HaeII r_GCGC'y Cuts at: 1678 2469 2773 4953 1678 Size: 791 304 2180 2531 Fragments arranged by size: 2531 2180 791 304 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2255 2287 4447 4479 4521 Size: 32 135 32 683 32 2160 32 42 Cuts at: 4521 4553 1373 Size: 32 2626 Fragments arranged by size: 2626 2160 683 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2848 3150 3396 669 Size: 988 281 910 302 246 3079 Fragments arranged by size: 3079 988 910 302 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5806 HpaI GTT'AAC Cuts at: 3396 3396 Size: 5806 KpnI G_GTAC'C Cuts at: 3131 3131 Size: 5806 MfeI C'AATT_G Cuts at: 3405 3405 Size: 5806 MluI A'CGCG_T Cuts at: 5682 5682 Size: 5806 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4920 5104 1305 Size: 3615 184 2007 Fragments arranged by size: 3615 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2141 2160 2380 2483 2573 Size: 54 1425 343 309 19 220 103 90 Cuts at: 2573 2813 10 Size: 240 3003 Fragments arranged by size: 3003 1425 343 309 240 220 103 90 54 19 MslI CAynn'nnrTG Cuts at: 518 1166 3764 4123 4282 518 Size: 648 2598 359 159 2042 Fragments arranged by size: 2598 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2584 2638 3222 3899 5047 5292 1606 Size: 978 54 584 677 1148 245 2120 Fragments arranged by size: 2120 1148 978 677 584 245 54 NcoI C'CATG_G Cuts at: 513 1065 2574 2881 513 Size: 552 1509 307 3438 Fragments arranged by size: 3438 1509 552 307 NdeI CA'TA_TG Cuts at: 387 387 Size: 5806 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5806 NotI GC'GGCC_GC Cuts at: 3253 3253 Size: 5806 NsiI A_TGCA'T Cuts at: 2304 2304 Size: 5806 NspI r_CATG'y Cuts at: 1132 1229 2185 2400 2434 3164 4709 1132 Size: 97 956 215 34 730 1545 2229 Fragments arranged by size: 2229 1545 956 730 215 97 34 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5806 PciI A'CATG_T Cuts at: 2430 4705 2430 Size: 2275 3531 PflMI CCAn_nnn'nTGG Cuts at: 1108 2623 2668 1108 Size: 1515 45 4246 Fragments arranged by size: 4246 1515 45 PfoI T'CCnGG_A Cuts at: 2273 2273 Size: 5806 PmeI GTTT'AAAC Cuts at: 3110 3110 Size: 5806 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3875 3907 5388 5420 3875 Size: 32 1481 32 4261 Fragments arranged by size: 4261 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2277 2277 Size: 5806 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5806 PsiI TTA'TAA Cuts at: 3376 3376 Size: 5806 PspOMI G'GGCC_C Cuts at: 3043 3043 Size: 5806 PstI C_TGCA'G Cuts at: 830 2637 3158 830 Size: 1807 521 3478 Fragments arranged by size: 3478 1807 521 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5806 PvuII CAG'CTG Cuts at: 2638 2638 Size: 5806 SacI G_AGCT'C Cuts at: 3083 3083 Size: 5806 SalI G'TCGA_C Cuts at: 1936 3148 1936 Size: 1212 4594 SanDI GG'GwC_CC Cuts at: 2277 2277 Size: 5806 SbfI CC_TGCA'GG Cuts at: 3158 3158 Size: 5806 ScaI AGT'ACT Cuts at: 4054 5766 4054 Size: 1712 4094 SfcI C'TryA_G Cuts at: 826 1045 2633 3154 4289 4970 5161 826 Size: 219 1588 521 1135 681 191 1471 Fragments arranged by size: 1588 1471 1135 681 521 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5806 SmaI CCC'GGG Cuts at: 1872 3133 1872 Size: 1261 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2507 2774 3038 3910 4811 5073 Size: 197 926 564 267 264 872 901 262 Cuts at: 5073 820 Size: 1553 Fragments arranged by size: 1553 926 901 872 564 267 264 262 197 SnaBI TAC'GTA Cuts at: 493 3597 493 Size: 3104 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5806 SphI G_CATG'C Cuts at: 1132 2400 3164 1132 Size: 1268 764 3774 Fragments arranged by size: 3774 1268 764 SspI AAT'ATT Cuts at: 5 52 3730 5 Size: 47 3678 2081 Fragments arranged by size: 3678 2081 47 StuI AGG'CCT Cuts at: 1517 2654 1517 Size: 1137 4669 StyI C'CwwG_G Cuts at: 513 1065 2574 2781 2881 3245 513 Size: 552 1509 207 100 364 3074 Fragments arranged by size: 3074 1509 552 364 207 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2545 2915 3833 3850 4003 4188 4633 Size: 1255 965 370 918 17 153 185 445 Cuts at: 4633 325 Size: 1498 Fragments arranged by size: 1498 1255 965 918 445 370 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4052 5764 96 Size: 274 80 33 51 1443 2075 1712 138 Fragments arranged by size: 2075 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5766 5798 5766 Size: 32 5774 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5806 XbaI T'CTAG_A Cuts at: 3142 3142 Size: 5806 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2090 2134 2381 2620 3100 2090 Size: 44 247 239 480 4796 Fragments arranged by size: 4796 480 247 239 44 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5806 XmaI C'CCGG_G Cuts at: 1870 3131 1870 Size: 1261 4545 XmnI GAAnn'nnTTC Cuts at: 3935 3935 Size: 5806 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3614 276 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I EcoRV FalI FseI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI