(Linear) (Six-base) MAPSORT of: KIBB8973.seq Check: 6270 from: 1 to: 1101 KIBB8973.orf With 182 enzymes: SgfI * December 27, 2011 16:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 460 721 1101 Size: 460 261 380 Fragments arranged by size: 460 380 261 AccI GT'mk_AC Cuts at: 0 350 854 1101 Size: 350 504 247 Fragments arranged by size: 504 350 247 AflIII A'CryG_T Cuts at: 0 284 425 1101 Size: 284 141 676 Fragments arranged by size: 676 284 141 AhdI GACnn_n'nnGTC Cuts at: 0 264 1101 Size: 264 837 AlwNI CAG_nnn'CTG Cuts at: 0 67 750 1101 Size: 67 683 351 Fragments arranged by size: 683 351 67 ApaI G_GGCC'C Cuts at: 0 1042 1101 Size: 1042 59 ApaLI G'TGCA_C Cuts at: 0 484 1101 Size: 484 617 BamHI G'GATC_C Cuts at: 0 25 1101 Size: 25 1076 BanII G_rGCy'C Cuts at: 0 592 1042 1078 1101 Size: 592 450 36 23 Fragments arranged by size: 592 450 36 23 BclI T'GATC_A Cuts at: 0 116 725 1101 Size: 116 609 376 Fragments arranged by size: 609 376 116 BfrBI ATG'CAT Cuts at: 0 297 1101 Size: 297 804 Bme1580I G_kGCm'C Cuts at: 0 488 833 957 1042 1101 Size: 488 345 124 85 59 Fragments arranged by size: 488 345 124 85 59 BmgBI CAC'GTC Cuts at: 0 840 1101 Size: 840 261 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 666 1101 Size: 666 435 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 57 190 1101 Size: 57 133 911 Fragments arranged by size: 911 133 57 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 487 754 1018 1101 Size: 487 267 264 83 Fragments arranged by size: 487 267 264 83 BsaI GGTCTCn'nnnn_ Cuts at: 0 251 1101 Size: 251 850 BsaAI yAC'GTr Cuts at: 0 858 1101 Size: 858 243 BsaHI Gr'CG_yC Cuts at: 0 163 1101 Size: 163 938 BseYI C'CCAG_C Cuts at: 0 896 1048 1101 Size: 896 152 53 Fragments arranged by size: 896 152 53 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 622 847 1101 Size: 622 225 254 Fragments arranged by size: 622 254 225 BsiEI CG_ry'CG Cuts at: 0 468 1101 Size: 468 633 BsiHKAI G_wGCw'C Cuts at: 0 488 1078 1101 Size: 488 590 23 Fragments arranged by size: 590 488 23 BsmBI CGTCTCn'nnnn_ Cuts at: 0 154 403 1101 Size: 154 249 698 Fragments arranged by size: 698 249 154 Bsp1286I G_dGCh'C Cuts at: 0 488 592 833 957 1042 1078 1101 Size: 488 104 241 124 85 36 23 Fragments arranged by size: 488 241 124 104 85 36 23 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 460 721 728 1101 Size: 460 261 7 373 Fragments arranged by size: 460 373 261 7 BsrBI CCG'CTC Cuts at: 0 848 1101 Size: 848 253 BsrDI GCAATG_nn' Cuts at: 0 154 1101 Size: 154 947 BsrFI r'CCGG_y Cuts at: 0 403 1101 Size: 403 698 BstAPI GCAn_nnn'nTGC Cuts at: 0 750 1101 Size: 750 351 BstBI TT'CG_AA Cuts at: 0 1055 1101 Size: 1055 46 BstEII G'GTnAC_C Cuts at: 0 708 1101 Size: 708 393 BstXI CCAn_nnnn'nTGG Cuts at: 0 345 361 804 816 1101 Size: 345 16 443 12 285 Fragments arranged by size: 443 345 285 16 12 BstYI r'GATC_y Cuts at: 0 25 1101 Size: 25 1076 BtgI C'CryG_G Cuts at: 0 137 305 569 704 876 1101 Size: 137 168 264 135 172 225 Fragments arranged by size: 264 225 172 168 137 135 BtsI GCAGTG_nn' Cuts at: 0 683 788 1101 Size: 683 105 313 Fragments arranged by size: 683 313 105 DraIII CAC_nnn'GTG Cuts at: 0 618 1101 Size: 618 483 EaeI y'GGCC_r Cuts at: 0 134 153 278 302 308 373 476 566 Size: 134 19 125 24 6 65 103 90 Cuts at: 566 806 1101 Size: 240 295 Fragments arranged by size: 295 240 134 125 103 90 65 24 19 6 EarI CTCTTCn'nnn_ Cuts at: 0 418 1101 Size: 418 683 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 985 1101 Size: 985 116 EcoICRI GAG'CTC Cuts at: 0 1076 1101 Size: 1076 25 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 57 190 1101 Size: 57 133 911 Fragments arranged by size: 911 133 57 EcoNI CCTnn'n_nnAGG Cuts at: 0 742 1101 Size: 742 359 EcoO109I rG'GnC_Cy Cuts at: 0 272 515 747 881 1038 1101 Size: 272 243 232 134 157 63 Fragments arranged by size: 272 243 232 157 134 63 FspI TGC'GCA Cuts at: 0 787 1101 Size: 787 314 FspAI rTGC'GCAy Cuts at: 0 787 1101 Size: 787 314 HaeII r_GCGC'y Cuts at: 0 464 768 1101 Size: 464 304 333 Fragments arranged by size: 464 333 304 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 250 282 1101 Size: 250 32 819 Fragments arranged by size: 819 250 32 HincII GTy'rAC Cuts at: 0 843 1101 Size: 843 258 MscI TGG'CCA Cuts at: 0 136 155 375 478 568 808 1101 Size: 136 19 220 103 90 240 293 Fragments arranged by size: 293 240 220 136 103 90 19 MspA1I CmG'CkG Cuts at: 0 579 633 1101 Size: 579 54 468 Fragments arranged by size: 579 468 54 NcoI C'CATG_G Cuts at: 0 569 876 1101 Size: 569 307 225 Fragments arranged by size: 569 307 225 NsiI A_TGCA'T Cuts at: 0 299 1101 Size: 299 802 NspI r_CATG'y Cuts at: 0 180 395 429 1101 Size: 180 215 34 672 Fragments arranged by size: 672 215 180 34 PciI A'CATG_T Cuts at: 0 425 1101 Size: 425 676 PflMI CCAn_nnn'nTGG Cuts at: 0 618 663 1101 Size: 618 45 438 Fragments arranged by size: 618 438 45 PfoI T'CCnGG_A Cuts at: 0 268 1101 Size: 268 833 PpuMI rG'GwC_Cy Cuts at: 0 272 1101 Size: 272 829 PspOMI G'GGCC_C Cuts at: 0 1038 1101 Size: 1038 63 PstI C_TGCA'G Cuts at: 0 632 1101 Size: 632 469 PvuII CAG'CTG Cuts at: 0 633 1101 Size: 633 468 SacI G_AGCT'C Cuts at: 0 1078 1101 Size: 1078 23 SanDI GG'GwC_CC Cuts at: 0 272 1101 Size: 272 829 SfcI C'TryA_G Cuts at: 0 628 1101 Size: 628 473 SmlI C'TyrA_G Cuts at: 0 502 769 1033 1101 Size: 502 267 264 68 Fragments arranged by size: 502 267 264 68 SphI G_CATG'C Cuts at: 0 395 1101 Size: 395 706 StuI AGG'CCT Cuts at: 0 649 1101 Size: 649 452 StyI C'CwwG_G Cuts at: 0 569 776 876 1101 Size: 569 207 100 225 Fragments arranged by size: 569 225 207 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 540 910 1101 Size: 540 370 191 Fragments arranged by size: 540 370 191 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 85 129 376 615 1101 Size: 85 44 247 239 486 Fragments arranged by size: 486 247 239 85 44 Enzymes that do cut: AarI AccI AflIII AhdI AlwNI ApaI ApaLI BamHI BanII BclI BfrBI Bme1580I BmgBI BplI BpmI BpuEI BsaI BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstEII BstXI BstYI BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I HincII MscI MspA1I NcoI NsiI NspI PciI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI SfcI SmlI SphI StuI StyI TaqII XcmI Enzymes that do not cut: AatII Acc65I AclI AcuI AfeI AflII AgeI AleI AlfI AloI ApoI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI BmrI BmtI Bpu10I BsaBI BsaWI BsaXI BseRI BsiWI BsmI BspEI BspHI BsrGI BssHII BssSI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EcoRI EcoRV FalI FseI HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI