(Circular) (Six-base) MAPSORT of: pFN21AE0036.seq Check: 5551 from: 1 to: 6850 pFN21AE0036 6850 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3113 3113 Size: 6850 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4660 278 Size: 53 83 186 936 3124 2468 Fragments arranged by size: 3124 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4193 1937 Size: 2256 4594 Acc65I G'GTAC_C Cuts at: 4171 4171 Size: 6850 AclI AA'CG_TT Cuts at: 1563 4977 5350 1563 Size: 3414 373 3063 Fragments arranged by size: 3414 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4054 4913 6297 6565 1632 Size: 2422 859 1384 268 1917 Fragments arranged by size: 2422 1917 1384 859 268 AfeI AGC'GCT Cuts at: 2423 3023 2423 Size: 600 6250 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6653 AflIII A'CryG_T Cuts at: 1203 5749 6726 1203 Size: 4546 977 1327 Fragments arranged by size: 4546 1327 977 AgeI A'CCGG_T Cuts at: 2239 4040 5681 2239 Size: 1801 1641 3408 Fragments arranged by size: 3408 1801 1641 AhdI GACnn_n'nnGTC Cuts at: 2221 5579 2221 Size: 3358 3492 AleI CACnn'nnGTG Cuts at: 1166 2351 1166 Size: 1185 5665 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2890 1184 Size: 1706 5144 AlwNI CAG_nnn'CTG Cuts at: 2397 2503 3954 4593 6165 2397 Size: 106 1451 639 1572 3082 Fragments arranged by size: 3082 1572 1451 639 106 ApaI G_GGCC'C Cuts at: 3046 3046 Size: 6850 ApaLI G'TGCA_C Cuts at: 4907 6063 4907 Size: 1156 5694 ApoI r'AATT_y Cuts at: 1493 3757 3814 4008 4159 4385 4537 6646 1493 Size: 2264 57 194 151 226 152 2109 1697 Fragments arranged by size: 2264 2109 1697 226 194 152 151 57 AseI AT'TA_AT Cuts at: 160 1032 5404 160 Size: 872 4372 1606 Fragments arranged by size: 4372 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6850 AvaI C'yCGr_G Cuts at: 1870 1943 3330 3885 3936 4089 4175 1870 Size: 73 1387 555 51 153 86 4545 Fragments arranged by size: 4545 1387 555 153 86 73 51 BamHI G'GATC_C Cuts at: 4180 4547 4180 Size: 367 6483 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2866 4171 5626 618 Size: 325 217 627 1079 1305 1455 1842 Fragments arranged by size: 1842 1455 1305 1079 627 325 217 BanII G_rGCy'C Cuts at: 1391 2668 3046 3624 3744 3886 3937 4169 1391 Size: 1277 378 578 120 142 51 232 4072 Fragments arranged by size: 4072 1277 578 378 232 142 120 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3391 928 Size: 971 1492 4387 Fragments arranged by size: 4387 1492 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2171 2205 3468 3502 5041 Size: 34 50 34 150 34 1263 34 1539 Cuts at: 5041 5075 1903 Size: 34 3678 Fragments arranged by size: 3678 1539 1263 150 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4124 4742 5952 4124 Size: 618 1210 5022 Fragments arranged by size: 5022 1210 618 BclI T'GATC_A Cuts at: 1548 1548 Size: 6850 BfrBI ATG'CAT Cuts at: 2008 2284 2008 Size: 276 6574 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2738 5461 136 Size: 107 122 71 631 605 1066 2723 1525 Fragments arranged by size: 2723 1525 1066 631 605 122 107 71 BglII A'GATC_T Cuts at: 6845 6845 Size: 6850 BlpI GC'TnA_GC Cuts at: 3770 3864 4267 3770 Size: 94 403 6353 Fragments arranged by size: 6353 403 94 Bme1580I G_kGCm'C Cuts at: 1790 2072 3046 3354 4911 6067 1790 Size: 282 974 308 1557 1156 2573 Fragments arranged by size: 2573 1557 1156 974 308 282 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6850 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4581 5539 442 Size: 416 53 83 399 24 3164 958 1753 Fragments arranged by size: 3164 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6850 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3419 5510 1239 Size: 2180 2091 2579 Fragments arranged by size: 2579 2180 2091 Bpu10I CC'TnA_GC Cuts at: 4001 4001 Size: 6850 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4975 5840 6138 4975 Size: 865 298 5687 Fragments arranged by size: 5687 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2093 2467 2965 5513 882 Size: 617 594 374 498 2548 2219 Fragments arranged by size: 2548 2219 617 594 498 374 BsaAI yAC'GTr Cuts at: 493 1204 4641 493 Size: 711 3437 2702 Fragments arranged by size: 3437 2702 711 BsaBI GATnn'nnATC Cuts at: 6844 6844 Size: 6850 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2934 3214 4657 5039 Size: 53 83 186 936 1401 280 1443 382 Cuts at: 5039 5674 275 Size: 635 1451 Fragments arranged by size: 1451 1443 1401 936 635 382 280 186 83 53 BsaWI w'CCGG_w Cuts at: 2239 2330 2985 4040 5283 5681 5955 6102 6513 Size: 91 655 1055 1243 398 274 147 411 Cuts at: 6513 2239 Size: 2576 Fragments arranged by size: 2576 1243 1055 655 411 398 274 147 91 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2066 2096 3458 3488 1178 Size: 30 165 30 663 30 1362 30 4540 Fragments arranged by size: 4540 1362 663 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2485 3074 3664 1186 Size: 1299 589 590 4372 Fragments arranged by size: 4372 1299 590 589 BseYI C'CCAG_C Cuts at: 2017 2055 2077 2322 2564 2778 3747 6053 6549 Size: 38 22 245 242 214 969 2306 496 Cuts at: 6549 2017 Size: 2318 Fragments arranged by size: 2318 2306 969 496 245 242 214 38 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3050 1163 Size: 573 1314 4963 Fragments arranged by size: 4963 1314 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2656 3040 4300 5061 6089 1540 Size: 275 186 655 384 1260 761 1028 2301 Fragments arranged by size: 2301 1260 1028 761 655 384 275 186 BsiHKAI G_wGCw'C Cuts at: 2404 3189 3624 4911 4996 6067 2404 Size: 785 435 1287 85 1071 3187 Fragments arranged by size: 3187 1287 1071 785 435 85 BsmI GAATG_Cn' Cuts at: 4361 4454 4361 Size: 93 6757 BsmBI CGTCTCn'nnnn_ Cuts at: 3205 3933 3205 Size: 728 6122 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2404 2668 3046 3189 3354 3624 Size: 399 282 332 264 378 143 165 270 Cuts at: 3624 3744 3886 3937 4169 4911 4996 6067 1391 Size: 120 142 51 232 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 378 332 282 270 264 232 165 143 142 120 85 51 BspEI T'CCGG_A Cuts at: 2330 6513 2330 Size: 4183 2667 BspHI T'CATG_A Cuts at: 4739 6469 4739 Size: 1730 5120 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2780 3072 3113 4205 844 Size: 1936 292 41 1092 3489 Fragments arranged by size: 3489 1936 1092 292 41 BsrBI CCG'CTC Cuts at: 1394 1823 2862 3096 4737 1394 Size: 429 1039 234 1641 3507 Fragments arranged by size: 3507 1641 1039 429 234 BsrDI GCAATG_nn' Cuts at: 65 1248 2584 5345 5519 65 Size: 1183 1336 2761 174 1396 Fragments arranged by size: 2761 1396 1336 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2239 3192 3630 4040 5494 5681 1700 Size: 110 429 953 438 410 1454 187 2869 Fragments arranged by size: 2869 1454 953 438 429 410 187 110 BsrGI T'GTAC_A Cuts at: 96 3246 96 Size: 3150 3700 BssHII G'CGCG_C Cuts at: 1925 3101 1925 Size: 1176 5674 BssSI C'ACGA_G Cuts at: 4910 5922 4910 Size: 1012 5838 BstAPI GCAn_nnn'nTGC Cuts at: 794 3111 794 Size: 2317 4533 BstBI TT'CG_AA Cuts at: 5654 5654 Size: 6850 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6850 BstXI CCAn_nnnn'nTGG Cuts at: 2140 2212 2393 2998 3613 2140 Size: 72 181 605 615 5377 Fragments arranged by size: 5377 615 605 181 72 BstYI r'GATC_y Cuts at: 1972 2805 4180 4547 4932 4949 6401 6487 6499 Size: 833 1375 367 385 17 1452 86 12 Cuts at: 6499 6845 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1375 833 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2211 2656 3276 3383 3700 4050 513 Size: 552 1146 445 620 107 317 350 3313 Fragments arranged by size: 3313 1146 620 552 445 350 317 107 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3844 487 Size: 683 56 2618 3493 Fragments arranged by size: 3493 2618 683 56 BtsI GCAGTG_nn' Cuts at: 2100 2687 2745 2959 4366 5160 5180 2100 Size: 587 58 214 1407 794 20 3770 Fragments arranged by size: 3770 1407 794 587 214 58 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6850 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6815 DraI TTT'AAA Cuts at: 4154 4154 Size: 6850 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5857 809 Size: 507 4541 1802 Fragments arranged by size: 4541 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2659 2730 Size: 54 1425 50 275 18 207 622 71 Cuts at: 2730 3009 3037 3198 3390 3421 3573 4297 5186 Size: 279 28 161 192 31 152 724 889 Cuts at: 5186 6815 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 724 622 279 275 207 192 161 152 71 54 50 43 31 28 18 EagI C'GGCC_G Cuts at: 1537 1812 3037 4297 1537 Size: 275 1225 1260 4090 Fragments arranged by size: 4090 1260 1225 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 2301 3903 3925 4780 1353 Size: 812 136 1602 22 855 3423 Fragments arranged by size: 3423 1602 855 812 136 22 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3541 5437 5811 5957 387 Size: 1064 138 1952 1896 374 146 1280 Fragments arranged by size: 1952 1896 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3622 3622 Size: 6850 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3419 4054 4913 5510 6297 6565 1239 Size: 393 1787 635 859 597 787 268 1524 Fragments arranged by size: 1787 1524 859 787 635 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2032 2487 2769 3043 3565 3893 3997 2032 Size: 455 282 274 522 328 104 4885 Fragments arranged by size: 4885 522 455 328 282 274 104 EcoRI G'AATT_C Cuts at: 4159 4537 4159 Size: 378 6472 FspI TGC'GCA Cuts at: 5356 5356 Size: 6850 HaeII r_GCGC'y Cuts at: 1678 2425 3025 3733 5997 1678 Size: 747 600 708 2264 2531 Fragments arranged by size: 2531 2264 747 708 600 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2066 2098 2207 2239 2525 Size: 32 135 32 494 32 109 32 286 Cuts at: 2525 2557 3119 3151 4065 4097 5491 5523 5565 Size: 32 562 32 914 32 1394 32 42 Cuts at: 5565 5597 1373 Size: 32 2626 Fragments arranged by size: 2626 1394 914 562 494 286 135 109 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2758 4194 4440 669 Size: 988 281 820 1436 246 3079 Fragments arranged by size: 3079 1436 988 820 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6850 HpaI GTT'AAC Cuts at: 4440 4440 Size: 6850 KpnI G_GTAC'C Cuts at: 4175 4175 Size: 6850 MfeI C'AATT_G Cuts at: 2158 4449 2158 Size: 2291 4559 MluI A'CGCG_T Cuts at: 6726 6726 Size: 6850 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2517 2637 2676 5964 6148 1305 Size: 1212 120 39 3288 184 2007 Fragments arranged by size: 3288 2007 1212 184 120 39 MscI TGG'CCA Cuts at: 10 64 1489 1832 2039 3011 3200 3392 3423 Size: 54 1425 343 207 972 189 192 31 Cuts at: 3423 3575 10 Size: 152 3285 Fragments arranged by size: 3285 1425 972 343 207 192 189 152 54 31 MslI CAynn'nnrTG Cuts at: 518 1166 2351 2675 2872 3611 4808 5167 5326 Size: 648 1185 324 197 739 1197 359 159 Cuts at: 5326 518 Size: 2042 Fragments arranged by size: 2042 1197 1185 739 648 359 324 197 159 MspA1I CmG'CkG Cuts at: 1606 2185 2196 2326 2371 2782 3522 4266 4943 Size: 579 11 130 45 411 740 744 677 Cuts at: 4943 6091 6336 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 744 740 677 579 411 245 130 45 11 NcoI C'CATG_G Cuts at: 513 1065 2211 3276 513 Size: 552 1146 1065 4087 Fragments arranged by size: 4087 1146 1065 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6850 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6850 NotI GC'GGCC_GC Cuts at: 4297 4297 Size: 6850 NsiI A_TGCA'T Cuts at: 2010 2286 2010 Size: 276 6574 NspI r_CATG'y Cuts at: 1132 1229 2288 3494 3685 4208 5753 1132 Size: 97 1059 1206 191 523 1545 2229 Fragments arranged by size: 2229 1545 1206 1059 523 191 97 PasI CC'CwG_GG Cuts at: 1348 2087 3346 1348 Size: 739 1259 4852 Fragments arranged by size: 4852 1259 739 PciI A'CATG_T Cuts at: 5749 5749 Size: 6850 PflMI CCAn_nnn'nTGG Cuts at: 1108 2503 2707 3796 1108 Size: 1395 204 1089 4162 Fragments arranged by size: 4162 1395 1089 204 PfoI T'CCnGG_A Cuts at: 2406 3624 2406 Size: 1218 5632 PmeI GTTT'AAAC Cuts at: 4154 4154 Size: 6850 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3908 3940 4919 4951 6432 6464 3908 Size: 32 979 32 1481 32 4294 Fragments arranged by size: 4294 1481 979 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2032 2769 2032 Size: 737 6113 PshAI GACnn'nnGTC Cuts at: 1613 2105 3078 1613 Size: 492 973 5385 Fragments arranged by size: 5385 973 492 PsiI TTA'TAA Cuts at: 4420 4420 Size: 6850 PspOMI G'GGCC_C Cuts at: 3042 3042 Size: 6850 PspXI vC'TCGA_Gb Cuts at: 3885 3885 Size: 6850 PstI C_TGCA'G Cuts at: 830 2429 2752 4109 4202 830 Size: 1599 323 1357 93 3478 Fragments arranged by size: 3478 1599 1357 323 93 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6850 PvuII CAG'CTG Cuts at: 2371 2782 3522 2371 Size: 411 740 5699 Fragments arranged by size: 5699 740 411 RsrII CG'GwC_CG Cuts at: 2328 2328 Size: 6850 SacI G_AGCT'C Cuts at: 3624 3624 Size: 6850 SalI G'TCGA_C Cuts at: 1936 4192 1936 Size: 2256 4594 SapI GCTCTTCn'nnn_ Cuts at: 2165 3925 2165 Size: 1760 5090 SbfI CC_TGCA'GG Cuts at: 4202 4202 Size: 6850 ScaI AGT'ACT Cuts at: 5098 6810 5098 Size: 1712 5138 SfcI C'TryA_G Cuts at: 826 1045 2425 2748 3355 4105 4198 5333 6014 Size: 219 1380 323 607 750 93 1135 681 Cuts at: 6014 6205 826 Size: 191 1471 Fragments arranged by size: 1471 1380 1135 750 681 607 323 219 191 93 SfiI GGCCn_nnn'nGGCC Cuts at: 2738 2738 Size: 6850 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6850 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6850 SmaI CCC'GGG Cuts at: 1872 3332 4177 1872 Size: 1460 845 4545 Fragments arranged by size: 4545 1460 845 SmlI C'TyrA_G Cuts at: 820 1017 1943 3885 4954 5855 6117 820 Size: 197 926 1942 1069 901 262 1553 Fragments arranged by size: 1942 1553 1069 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4641 493 Size: 4148 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6850 SphI G_CATG'C Cuts at: 1132 4208 1132 Size: 3076 3774 SrfI GCCC'GGGC Cuts at: 3332 3332 Size: 6850 SspI AAT'ATT Cuts at: 5 52 4774 5 Size: 47 4722 2081 Fragments arranged by size: 4722 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6850 StyI C'CwwG_G Cuts at: 513 1065 2211 3276 4289 513 Size: 552 1146 1065 1013 3074 Fragments arranged by size: 3074 1146 1065 1013 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2095 2827 3496 4877 4894 5047 5232 Size: 1255 515 732 669 1381 17 153 185 Cuts at: 5232 5677 325 Size: 445 1498 Fragments arranged by size: 1498 1381 1255 732 669 515 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3246 5096 6808 Size: 274 80 33 51 1443 1269 1850 1712 Cuts at: 6808 96 Size: 138 Fragments arranged by size: 1850 1712 1443 1269 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6810 6842 6810 Size: 32 6818 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6850 XbaI T'CTAG_A Cuts at: 4186 4186 Size: 6850 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2140 2500 3343 2140 Size: 360 843 5647 Fragments arranged by size: 5647 843 360 XhoI C'TCGA_G Cuts at: 1943 3885 1943 Size: 1942 4908 XmaI C'CCGG_G Cuts at: 1870 3330 4175 1870 Size: 1460 845 4545 Fragments arranged by size: 4545 1460 845 XmnI GAAnn'nnTTC Cuts at: 3923 4979 3923 Size: 1056 5794 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4658 276 Size: 53 83 186 936 3124 2468 Fragments arranged by size: 3124 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI SacII SanDI SexAI SfoI SwaI