Restriction map for
pFN21AB7317
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# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspHI AspI AvrII BbrPI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsiHKAI BsiWI Bsp1407I BspDI
BspLU11I BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
DraI DraIII DrdI Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI MfeI MluI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI SacI SapI SbfI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 111 GTCGAC GT^[AC][GT]AC
AccIII 2750 TCCGGA T^CCGGA
AcsI 96 GAATTT [GA]^AATT[CT]
AhdI 955 GACCCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 848 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2172 CCTGAGG CC^T[ACGT]AGG
ApoI 96 GAATTT [GA]^AATT[CT]
AspEI 955 GACCCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1901 GGATCC G^GATCC
BbeI 277 GGCGCC GGCGC^C
BbsI 1777 GTCTTC GAAGAC(2/6)
BfaI 1898 CTAG C^TAG
BpuAI 1777 GTCTTC GAAGAC(2/6)
BsaWI 2750 TCCGGA [AT]^CCGG[AT]
BseAI 2750 TCCGGA T^CCGGA
BsmI 2960 GCATTC GAATGC(1/-1)
BspEI 2750 TCCGGA T^CCGGA
BspHI 224 TCATGA T^CATGA
Bsu36I 2172 CCTGAGG CC^T[ACGT]AGG
CpoI 386 CGGACCG CG^G[AT]CCG
Eam1105I 955 GACCCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1692 GAAGAG CTCTTC(1/4)
Eco81I 2172 CCTGAGG CC^T[ACGT]AGG
HaeII 277 GGCGCC [GA]GCGC^[CT]
KasI 277 GGCGCC G^GCGCC
Ksp632I 1692 GAAGAG CTCTTC(1/4)
MaeI 1898 CTAG C^TAG
MroI 2750 TCCGGA T^CCGGA
NarI 277 GGCGCC GG^CGCC
PaeR7I 2095 CTCGAG C^TCGAG
PstI 738 CTGCAG CTGCA^G
RcaI 224 TCATGA T^CATGA
RsrII 386 CGGACCG CG^G[AT]CCG
SalI 111 GTCGAC G^TCGAC
ScaI 1427 AGTACT AGT^ACT
SfoI 277 GGCGCC GGC^GCC
XhoI 2095 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
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Acc65I 2561 GGTACC G^GTACC
2837 GGTACC
Asp718I 2561 GGTACC G^GTACC
2837 GGTACC
BciVI 2863 GGATAC GTATCC(6/5)
144 GTATCC
BsaBI 1902 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2397 GATCCCCATC
BsiEI 47 CGGCCG CG[GA][CT]^CG
446 CGGCCG
BsrDI 441 CATTGC GCAATG(2/0)
2125 GCAATG
BssHII 82 GCGCGC G^CGCGC
2910 GCGCGC
BssSI 255 CACGAG CACGAG(-5/-1)
760 CTCGTG
EagI 47 CGGCCG C^GGCCG
446 CGGCCG
EclXI 47 CGGCCG C^GGCCG
446 CGGCCG
Eco52I 47 CGGCCG C^GGCCG
446 CGGCCG
KpnI 2561 GGTACC GGTAC^C
2837 GGTACC
KspI 50 CCGCGG CCGC^GG
77 CCGCGG
MamI 1902 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2397 GATCCCCATC
PshAI 1359 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1623 GACCATTGTC
SacII 50 CCGCGG CCGC^GG
77 CCGCGG
SexAI 209 ACCAGGT A^CC[AT]GGT
2312 ACCTGGT
SfcI 738 CTGCAG C^T[GA][CT]AG
1707 CTACAG
SmaI 11 CCCGGG CCC^GGG
1800 CCCGGG
StuI 1715 AGGCCT AGG^CCT
1892 AGGCCT
Tsp509I 97 AATT ^AATT
2069 AATT
XmaCI 11 CCCGGG C^CCGGG
1800 CCCGGG
XmaI 11 CCCGGG C^CCGGG
1800 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI ApaI AvaI AvaII
AviII BalI BanI BanII BbvCI
BbvI BcgI BcnI BglI BmyI
BpmI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfiI
SmlI SphI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII