(Circular) (Six-base) MAPSORT of: pFN21AB7317.seq Check: 1750 from: 1 to: 7720 pFN21AB7317 7720 bp With 182 enzymes: SgfI * April 15, 2010 09:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2081 2363 4722 2081 Size: 282 2359 5079 Fragments arranged by size: 5079 2359 282 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5530 278 Size: 53 83 186 936 3994 2468 Fragments arranged by size: 3994 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2117 5063 1937 Size: 180 2946 4594 Fragments arranged by size: 4594 2946 180 Acc65I G'GTAC_C Cuts at: 4566 4842 5041 4566 Size: 276 199 7245 Fragments arranged by size: 7245 276 199 AclI AA'CG_TT Cuts at: 1563 5847 6220 1563 Size: 4284 373 3063 Fragments arranged by size: 4284 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3556 5783 7167 7435 1632 Size: 1924 2227 1384 268 1917 Fragments arranged by size: 2227 1924 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7523 AflIII A'CryG_T Cuts at: 1203 2112 2208 3528 4464 6619 7596 1203 Size: 909 96 1320 936 2155 977 1327 Fragments arranged by size: 2155 1327 1320 977 936 909 96 AgeI A'CCGG_T Cuts at: 6551 6551 Size: 7720 AhdI GACnn_n'nnGTC Cuts at: 2965 6449 2965 Size: 3484 4236 AleI CACnn'nnGTG Cuts at: 1166 2075 2510 1166 Size: 909 435 6376 Fragments arranged by size: 6376 909 435 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2351 1184 Size: 1167 6553 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2996 3028 2996 Size: 32 7688 AlwNI CAG_nnn'CTG Cuts at: 2858 5463 7035 2858 Size: 2605 1572 3543 Fragments arranged by size: 3543 2605 1572 ApaI G_GGCC'C Cuts at: 3052 4984 5007 3052 Size: 1932 23 5765 Fragments arranged by size: 5765 1932 23 ApaLI G'TGCA_C Cuts at: 5777 6933 5777 Size: 1156 6564 ApoI r'AATT_y Cuts at: 1493 2101 5029 5255 5407 7516 1493 Size: 608 2928 226 152 2109 1697 Fragments arranged by size: 2928 2109 1697 608 226 152 AseI AT'TA_AT Cuts at: 160 1032 6274 160 Size: 872 5242 1606 Fragments arranged by size: 5242 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7720 AvaI C'yCGr_G Cuts at: 1870 1943 2016 2274 3272 3392 3805 3885 4100 Size: 73 73 258 998 120 413 80 215 Cuts at: 4100 5045 1870 Size: 945 4545 Fragments arranged by size: 4545 998 945 413 258 215 120 80 73 73 BamHI G'GATC_C Cuts at: 3906 5050 5417 3906 Size: 1144 367 6209 Fragments arranged by size: 6209 1144 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2282 2351 2471 2570 2995 Size: 325 217 627 495 69 120 99 425 Cuts at: 2995 3149 3518 3769 3970 4238 4566 4734 4842 Size: 154 369 251 201 268 328 168 108 Cuts at: 4842 5013 5041 6496 618 Size: 171 28 1455 1842 Fragments arranged by size: 1842 1455 627 495 425 369 328 325 268 251 217 201 171 168 154 120 108 99 69 28 BanII G_rGCy'C Cuts at: 1391 2237 3052 3272 3279 3393 3547 3825 4347 Size: 846 815 220 7 114 154 278 522 Cuts at: 4347 4984 5007 5039 1391 Size: 637 23 32 4072 Fragments arranged by size: 4072 846 815 637 522 278 220 154 114 32 23 7 BbeI G_GCGC'C Cuts at: 2286 2286 Size: 7720 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3775 928 Size: 971 1876 4873 Fragments arranged by size: 4873 1876 971 BbvCI CC'TCA_GC Cuts at: 2531 2936 2971 3553 2531 Size: 405 35 582 6698 Fragments arranged by size: 6698 582 405 35 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2429 2463 4355 4389 4634 4668 5911 Size: 34 492 34 1892 34 245 34 1243 Cuts at: 5911 5945 1903 Size: 34 3678 Fragments arranged by size: 3678 1892 1243 492 245 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2160 4862 5612 6822 2160 Size: 2702 750 1210 3058 Fragments arranged by size: 3058 2702 1210 750 BclI T'GATC_A Cuts at: 1548 1548 Size: 7720 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2060 2579 2825 Size: 107 122 71 631 605 388 519 246 Cuts at: 2825 3870 4004 4427 5011 6331 136 Size: 1045 134 423 584 1320 1525 Fragments arranged by size: 1525 1320 1045 631 605 584 519 423 388 246 134 122 107 71 BglII A'GATC_T Cuts at: 7715 7715 Size: 7720 BlpI GC'TnA_GC Cuts at: 5137 5137 Size: 7720 Bme1580I G_kGCm'C Cuts at: 1790 2354 2618 2772 2878 3052 3121 3165 3521 Size: 564 264 154 106 174 69 44 356 Cuts at: 3521 3993 4739 4984 5007 5781 6937 1790 Size: 472 746 245 23 774 1156 2573 Fragments arranged by size: 2573 1156 774 746 564 472 356 264 245 174 154 106 69 44 23 BmgBI CAC'GTC Cuts at: 1318 2115 4465 4912 1318 Size: 797 2350 447 4126 Fragments arranged by size: 4126 2350 797 447 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2143 3820 5451 Size: 416 53 83 399 24 726 1677 1631 Cuts at: 5451 6409 442 Size: 958 1753 Fragments arranged by size: 1753 1677 1631 958 726 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7720 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4177 4177 Size: 7720 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2405 2892 2998 3062 3437 3954 4379 6380 Size: 1166 487 106 64 375 517 425 2001 Cuts at: 6380 1239 Size: 2579 Fragments arranged by size: 2579 2001 1166 517 487 425 375 106 64 Bpu10I CC'TnA_GC Cuts at: 2531 2936 2971 3266 3553 4049 2531 Size: 405 35 295 287 496 6202 Fragments arranged by size: 6202 496 405 295 287 35 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2480 3458 3494 5845 6710 7008 2480 Size: 978 36 2351 865 298 3192 Fragments arranged by size: 3192 2351 978 865 298 36 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2357 3587 4275 4842 6383 882 Size: 617 858 1230 688 567 1541 2219 Fragments arranged by size: 2219 1541 1230 858 688 617 567 BsaAI yAC'GTr Cuts at: 493 1204 2148 2211 3529 5511 493 Size: 711 944 63 1318 1982 2702 Fragments arranged by size: 2702 1982 1318 944 711 63 BsaBI GATnn'nnATC Cuts at: 3911 4406 7714 3911 Size: 495 3308 3917 Fragments arranged by size: 3917 3308 495 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2283 2491 4058 5527 Size: 53 83 186 936 750 208 1567 1469 Cuts at: 5527 5909 6544 275 Size: 382 635 1451 Fragments arranged by size: 1567 1469 1451 936 750 635 382 208 186 83 53 BsaWI w'CCGG_w Cuts at: 4755 6153 6551 6825 6972 7383 4755 Size: 1398 398 274 147 411 5092 Fragments arranged by size: 5092 1398 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2744 2774 2882 2912 2996 Size: 30 165 30 1341 30 108 30 84 Cuts at: 2996 3026 4550 4580 1178 Size: 30 1524 30 4318 Fragments arranged by size: 4318 1524 1341 165 108 84 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2732 2819 3755 3869 4245 4441 4574 4864 Size: 1546 87 936 114 376 196 133 290 Cuts at: 4864 1186 Size: 4042 Fragments arranged by size: 4042 1546 936 376 290 196 133 114 87 BseYI C'CCAG_C Cuts at: 2265 2347 2836 3165 4983 6923 7419 2265 Size: 82 489 329 1818 1940 496 2566 Fragments arranged by size: 2566 1940 1818 496 489 329 82 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2809 2962 2970 3203 4589 1163 Size: 573 1073 153 8 233 1386 4294 Fragments arranged by size: 4294 1386 1073 573 233 153 8 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2055 2454 5170 5931 6959 1540 Size: 275 186 54 399 2716 761 1028 2301 Fragments arranged by size: 2716 2301 1028 761 399 275 186 54 BsiHKAI G_wGCw'C Cuts at: 5781 5866 6937 5781 Size: 85 1071 6564 Fragments arranged by size: 6564 1071 85 BsmI GAATG_Cn' Cuts at: 4965 5231 5324 4965 Size: 266 93 7361 Fragments arranged by size: 7361 266 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2271 2498 3376 3505 2271 Size: 227 878 129 6486 Fragments arranged by size: 6486 878 227 129 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2354 2618 2772 2878 3052 3121 Size: 399 447 117 264 154 106 174 69 Cuts at: 3121 3165 3272 3279 3393 3521 3547 3825 3993 Size: 44 107 7 114 128 26 278 168 Cuts at: 3993 4347 4739 4984 5007 5039 5781 5866 6937 Size: 354 392 245 23 32 742 85 1071 Cuts at: 6937 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 447 399 392 354 278 264 245 174 168 154 128 117 114 107 106 85 69 44 32 26 23 7 BspEI T'CCGG_A Cuts at: 4755 7383 4755 Size: 2628 5092 BspHI T'CATG_A Cuts at: 2229 5609 7339 2229 Size: 3380 1730 2610 Fragments arranged by size: 3380 2610 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2081 2363 3216 4722 5075 844 Size: 1237 282 853 1506 353 3489 Fragments arranged by size: 3489 1506 1237 853 353 282 BsrBI CCG'CTC Cuts at: 1394 1823 2722 2809 3147 3352 4513 5607 1394 Size: 429 899 87 338 205 1161 1094 3507 Fragments arranged by size: 3507 1161 1094 899 429 338 205 87 BsrDI GCAATG_nn' Cuts at: 65 1248 2445 4137 6215 6389 65 Size: 1183 1197 1692 2078 174 1396 Fragments arranged by size: 2078 1692 1396 1197 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2795 4016 4241 4839 5010 6364 6551 Size: 110 985 1221 225 598 171 1354 187 Cuts at: 6551 1700 Size: 2869 Fragments arranged by size: 2869 1354 1221 985 598 225 187 171 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7720 BssHII G'CGCG_C Cuts at: 1925 2087 4915 1925 Size: 162 2828 4730 Fragments arranged by size: 4730 2828 162 BssSI C'ACGA_G Cuts at: 2260 2765 5780 6792 2260 Size: 505 3015 1012 3188 Fragments arranged by size: 3188 3015 1012 505 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7720 BstBI TT'CG_AA Cuts at: 6524 6524 Size: 7720 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7720 BstXI CCAn_nnnn'nTGG Cuts at: 3196 3211 3589 4834 3196 Size: 15 378 1245 6082 Fragments arranged by size: 6082 1245 378 15 BstYI r'GATC_y Cuts at: 1972 3639 3906 4401 4953 5050 5417 5802 5819 Size: 1667 267 495 552 97 367 385 17 Cuts at: 5819 7271 7357 7369 7715 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1667 1452 552 495 385 367 346 267 97 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4178 4178 Size: 7720 BtgI C'CryG_G Cuts at: 513 1065 2004 2055 2082 2125 2622 3582 3993 Size: 552 939 51 27 43 497 960 411 Cuts at: 3993 4117 4389 4584 4806 513 Size: 124 272 195 222 3427 Fragments arranged by size: 3427 960 939 552 497 411 272 222 195 124 51 43 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4417 487 Size: 683 56 3191 3790 Fragments arranged by size: 3790 3191 683 56 BtsI GCAGTG_nn' Cuts at: 2524 2661 2797 3082 5236 6030 6050 2524 Size: 137 136 285 2154 794 20 4194 Fragments arranged by size: 4194 2154 794 285 137 136 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7720 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7685 DraI TTT'AAA Cuts at: 5024 5024 Size: 7720 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6727 809 Size: 507 5411 1802 Fragments arranged by size: 5411 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2067 2451 Size: 54 1425 50 275 18 222 15 384 Cuts at: 2451 2580 2984 3070 3201 3565 3996 4120 4287 Size: 129 404 86 131 364 431 124 167 Cuts at: 4287 4455 5167 6056 7685 8 Size: 168 712 889 1629 43 Fragments arranged by size: 1629 1425 889 712 431 404 384 364 275 222 168 167 131 129 124 86 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2052 2451 5167 1537 Size: 275 240 399 2716 4090 Fragments arranged by size: 4090 2716 399 275 240 EarI CTCTTCn'nnn_ Cuts at: 1353 3692 5650 1353 Size: 2339 1958 3423 Fragments arranged by size: 3423 2339 1958 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4085 4881 6307 6681 6827 387 Size: 1064 138 2496 796 1426 374 146 1280 Fragments arranged by size: 2496 1426 1280 1064 796 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2405 2892 2998 3062 3437 3556 3954 Size: 393 773 487 106 64 375 119 398 Cuts at: 3954 4379 5783 6380 7167 7435 1239 Size: 425 1404 597 787 268 1524 Fragments arranged by size: 1524 1404 787 773 597 487 425 398 393 375 268 119 106 64 EcoNI CCTnn'n_nnAGG Cuts at: 3182 4052 4177 3182 Size: 870 125 6725 Fragments arranged by size: 6725 870 125 EcoO109I rG'GnC_Cy Cuts at: 2269 2525 3049 4006 4182 4798 5003 5004 2269 Size: 256 524 957 176 616 205 1 4985 Fragments arranged by size: 4985 957 616 524 256 205 176 1 EcoRI G'AATT_C Cuts at: 5029 5407 5029 Size: 378 7342 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2383 2560 2383 Size: 177 7543 FspI TGC'GCA Cuts at: 2258 2516 2630 6226 2258 Size: 258 114 3596 3752 Fragments arranged by size: 3752 3596 258 114 HaeII r_GCGC'y Cuts at: 1678 2286 6867 1678 Size: 608 4581 2531 Fragments arranged by size: 4581 2531 608 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2660 2692 2951 2983 3483 Size: 32 135 32 1088 32 259 32 500 Cuts at: 3483 3515 3739 3771 6361 6393 6435 6467 1373 Size: 32 224 32 2590 32 42 32 2626 Fragments arranged by size: 2626 2590 1088 500 259 224 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2118 3298 4309 5064 5310 669 Size: 988 281 180 1180 1011 755 246 3079 Fragments arranged by size: 3079 1180 1011 988 755 281 246 180 HindIII A'AGCT_T Cuts at: 748 748 Size: 7720 HpaI GTT'AAC Cuts at: 5310 5310 Size: 7720 KasI G'GCGC_C Cuts at: 2282 2282 Size: 7720 KpnI G_GTAC'C Cuts at: 4570 4846 5045 4570 Size: 276 199 7245 Fragments arranged by size: 7245 276 199 MfeI C'AATT_G Cuts at: 5319 5319 Size: 7720 MluI A'CGCG_T Cuts at: 7596 7596 Size: 7720 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2370 3285 6834 7018 1305 Size: 1065 915 3549 184 2007 Fragments arranged by size: 3549 2007 1065 915 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2582 3203 3998 10 Size: 54 1425 343 750 621 795 3732 Fragments arranged by size: 3732 1425 795 750 621 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2075 2510 3587 4205 4832 4934 5678 Size: 648 909 435 1077 618 627 102 744 Cuts at: 5678 6037 6196 518 Size: 359 159 2042 Fragments arranged by size: 2042 1077 909 744 648 627 618 435 359 159 102 MspA1I CmG'CkG Cuts at: 1606 2057 2084 2869 2880 3108 3129 3290 3982 Size: 451 27 785 11 228 21 161 692 Cuts at: 3982 4213 4413 4648 4785 4987 5136 5813 6961 Size: 231 200 235 137 202 149 677 1148 Cuts at: 6961 7206 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 785 692 677 451 245 235 231 228 202 200 161 149 137 27 21 11 NaeI GCC'GGC Cuts at: 2797 4018 4243 5012 2797 Size: 1221 225 769 5505 Fragments arranged by size: 5505 1221 769 225 NarI GG'CG_CC Cuts at: 2283 2283 Size: 7720 NcoI C'CATG_G Cuts at: 513 1065 2004 2622 3582 4584 513 Size: 552 939 618 960 1002 3649 Fragments arranged by size: 3649 1002 960 939 618 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7720 NgoMIV G'CCGG_C Cuts at: 2795 4016 4241 5010 2795 Size: 1221 225 769 5505 Fragments arranged by size: 5505 1221 769 225 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7720 NotI GC'GGCC_GC Cuts at: 5167 5167 Size: 7720 NspI r_CATG'y Cuts at: 1132 1229 2338 3141 4225 4249 5078 6623 1132 Size: 97 1109 803 1084 24 829 1545 2229 Fragments arranged by size: 2229 1545 1109 1084 829 803 97 24 PasI CC'CwG_GG Cuts at: 1348 2858 3114 4921 4999 1348 Size: 1510 256 1807 78 4069 Fragments arranged by size: 4069 1807 1510 256 78 PciI A'CATG_T Cuts at: 6619 6619 Size: 7720 PflMI CCAn_nnn'nTGG Cuts at: 1108 3334 3583 3636 4413 1108 Size: 2226 249 53 777 4415 Fragments arranged by size: 4415 2226 777 249 53 PfoI T'CCnGG_A Cuts at: 2559 3414 3726 2559 Size: 855 312 6553 Fragments arranged by size: 6553 855 312 PmeI GTTT'AAAC Cuts at: 5024 5024 Size: 7720 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2996 3028 3812 3844 5789 5821 7302 7334 2996 Size: 32 784 32 1945 32 1481 32 3382 Fragments arranged by size: 3382 1945 1481 784 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2269 2525 4182 2269 Size: 256 1657 5807 Fragments arranged by size: 5807 1657 256 PshAI GACnn'nnGTC Cuts at: 1613 3368 3632 1613 Size: 1755 264 5701 Fragments arranged by size: 5701 1755 264 PsiI TTA'TAA Cuts at: 5290 5290 Size: 7720 PspOMI G'GGCC_C Cuts at: 3048 4980 5003 3048 Size: 1932 23 5765 Fragments arranged by size: 5765 1932 23 PspXI vC'TCGA_Gb Cuts at: 4100 4100 Size: 7720 PstI C_TGCA'G Cuts at: 830 2747 5072 830 Size: 1917 2325 3478 Fragments arranged by size: 3478 2325 1917 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7720 PvuII CAG'CTG Cuts at: 2869 4213 4987 2869 Size: 1344 774 5602 Fragments arranged by size: 5602 1344 774 RsrII CG'GwC_CG Cuts at: 2392 2392 Size: 7720 SacII CC_GC'GG Cuts at: 2058 2085 2058 Size: 27 7693 SalI G'TCGA_C Cuts at: 1936 2116 5062 1936 Size: 180 2946 4594 Fragments arranged by size: 4594 2946 180 SanDI GG'GwC_CC Cuts at: 2269 2525 2269 Size: 256 7464 SbfI CC_TGCA'GG Cuts at: 5072 5072 Size: 7720 ScaI AGT'ACT Cuts at: 3434 5968 7680 3434 Size: 2534 1712 3474 Fragments arranged by size: 3474 2534 1712 SexAI A'CCwGG_T Cuts at: 2214 4317 2214 Size: 2103 5617 SfcI C'TryA_G Cuts at: 826 1045 2743 3712 5068 6203 6884 7075 826 Size: 219 1698 969 1356 1135 681 191 1471 Fragments arranged by size: 1698 1471 1356 1135 969 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3870 4004 4427 3870 Size: 134 423 7163 Fragments arranged by size: 7163 423 134 SfoI GGC'GCC Cuts at: 2284 2284 Size: 7720 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7720 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7720 SmaI CCC'GGG Cuts at: 1872 2018 3807 5047 1872 Size: 146 1789 1240 4545 Fragments arranged by size: 4545 1789 1240 146 SmlI C'TyrA_G Cuts at: 820 1017 1943 2495 3473 3509 4100 5824 6725 Size: 197 926 552 978 36 591 1724 901 Cuts at: 6725 6987 820 Size: 262 1553 Fragments arranged by size: 1724 1553 978 926 901 591 552 262 197 36 SnaBI TAC'GTA Cuts at: 493 5511 493 Size: 5018 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7720 SphI G_CATG'C Cuts at: 1132 2338 4225 4249 5078 1132 Size: 1206 1887 24 829 3774 Fragments arranged by size: 3774 1887 1206 829 24 SspI AAT'ATT Cuts at: 5 52 5644 5 Size: 47 5592 2081 Fragments arranged by size: 5592 2081 47 StuI AGG'CCT Cuts at: 1517 3722 3899 1517 Size: 2205 177 5338 Fragments arranged by size: 5338 2205 177 StyI C'CwwG_G Cuts at: 513 1065 2004 2479 2622 3582 4584 5159 513 Size: 552 939 475 143 960 1002 575 3074 Fragments arranged by size: 3074 1002 960 939 575 552 475 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2151 2746 4226 4597 5012 5747 5764 Size: 1255 571 595 1480 371 415 735 17 Cuts at: 5764 5917 6102 6547 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1480 1255 735 595 571 445 415 371 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2739 3432 4146 Size: 274 80 33 51 1443 762 693 714 Cuts at: 4146 5966 7678 96 Size: 1820 1712 138 Fragments arranged by size: 1820 1712 1443 762 714 693 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2744 2776 2855 2882 2887 2914 3768 3800 7680 Size: 32 79 27 5 27 854 32 3880 Cuts at: 7680 7712 2744 Size: 32 2752 Fragments arranged by size: 3880 2752 854 79 32 32 32 27 27 5 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7720 XbaI T'CTAG_A Cuts at: 5056 5056 Size: 7720 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2179 3196 4210 2179 Size: 1017 1014 5689 Fragments arranged by size: 5689 1017 1014 XhoI C'TCGA_G Cuts at: 1943 4100 1943 Size: 2157 5563 XmaI C'CCGG_G Cuts at: 1870 2016 3805 5045 1870 Size: 146 1789 1240 4545 Fragments arranged by size: 4545 1789 1240 146 XmnI GAAnn'nnTTC Cuts at: 5849 5849 Size: 7720 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5528 276 Size: 53 83 186 936 3994 2468 Fragments arranged by size: 3994 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI SacI SapI SrfI SwaI