Restriction map for
pFN21AB7317
(Linear) (Six-base) MAPSORT of: KIBB7317.seq Check: 1232 from: 1 to: 3015
KIBB7317.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 76 358 2717 3015
Size: 76 282 2359 298
Fragments arranged by size:
2359 298 282 76
AccI GT'mk_AC
Cuts at: 0 112 3015
Size: 112 2903
Acc65I G'GTAC_C
Cuts at: 0 2561 2837 3015
Size: 2561 276 178
Fragments arranged by size:
2561 276 178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1551 3015
Size: 1551 1464
AflIII A'CryG_T
Cuts at: 0 107 203 1523 2459 3015
Size: 107 96 1320 936 556
Fragments arranged by size:
1320 936 556 107 96
AhdI GACnn_n'nnGTC
Cuts at: 0 960 3015
Size: 960 2055
AleI CACnn'nnGTG
Cuts at: 0 70 505 3015
Size: 70 435 2510
Fragments arranged by size:
2510 435 70
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 346 3015
Size: 346 2669
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 991 1023 3015
Size: 991 32 1992
Fragments arranged by size:
1992 991 32
AlwNI CAG_nnn'CTG
Cuts at: 0 853 3015
Size: 853 2162
ApaI G_GGCC'C
Cuts at: 0 1047 2979 3002 3015
Size: 1047 1932 23 13
Fragments arranged by size:
1932 1047 23 13
ApoI r'AATT_y
Cuts at: 0 96 3015
Size: 96 2919
AvaI C'yCGr_G
Cuts at: 0 11 269 1267 1387 1800 1880 2095 3015
Size: 11 258 998 120 413 80 215 920
Fragments arranged by size:
998 920 413 258 215 120 80 11
BamHI G'GATC_C
Cuts at: 0 1901 3015
Size: 1901 1114
BanI G'GyrC_C
Cuts at: 0 277 346 466 565 990 1144 1513 1764
Size: 277 69 120 99 425 154 369 251
Cuts at: 1764 1965 2233 2561 2729 2837 3008 3015
Size: 201 268 328 168 108 171 7
Fragments arranged by size:
425 369 328 277 268 251 201 171
168 154 120 108 99 69 7
BanII G_rGCy'C
Cuts at: 0 232 1047 1267 1274 1388 1542 1820 2342
Size: 232 815 220 7 114 154 278 522
Cuts at: 2342 2979 3002 3015
Size: 637 23 13
Fragments arranged by size:
815 637 522 278 232 220 154 114
23 13 7
BbeI G_GCGC'C
Cuts at: 0 281 3015
Size: 281 2734
BbsI GAAGACnn'nnnn_
Cuts at: 0 1770 3015
Size: 1770 1245
BbvCI CC'TCA_GC
Cuts at: 0 526 931 966 1548 3015
Size: 526 405 35 582 1467
Fragments arranged by size:
1467 582 526 405 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 424 458 2350 2384 2629 2663 3015
Size: 424 34 1892 34 245 34 352
Fragments arranged by size:
1892 424 352 245 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 155 2857 3015
Size: 155 2702 158
Fragments arranged by size:
2702 158 155
BglI GCCn_nnn'nGGC
Cuts at: 0 55 574 820 1865 1999 2422 3006 3015
Size: 55 519 246 1045 134 423 584 9
Fragments arranged by size:
1045 584 519 423 246 134 55 9
Bme1580I G_kGCm'C
Cuts at: 0 349 613 767 873 1047 1116 1160 1516
Size: 349 264 154 106 174 69 44 356
Cuts at: 1516 1988 2734 2979 3002 3015
Size: 472 746 245 23 13
Fragments arranged by size:
746 472 356 349 264 245 174 154
106 69 44 23 13
BmgBI CAC'GTC
Cuts at: 0 110 2460 2907 3015
Size: 110 2350 447 108
Fragments arranged by size:
2350 447 110 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 138 1815 3015
Size: 138 1677 1200
Fragments arranged by size:
1677 1200 138
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2172 3015
Size: 2172 843
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 887 993 1057 1432 1949 2374 3015
Size: 400 487 106 64 375 517 425 641
Fragments arranged by size:
641 517 487 425 400 375 106 64
Bpu10I CC'TnA_GC
Cuts at: 0 526 931 966 1261 1548 2044 3015
Size: 526 405 35 295 287 496 971
Fragments arranged by size:
971 526 496 405 295 287 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1453 1489 3015
Size: 475 978 36 1526
Fragments arranged by size:
1526 978 475 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 352 1582 2270 2837 3015
Size: 352 1230 688 567 178
Fragments arranged by size:
1230 688 567 352 178
BsaAI yAC'GTr
Cuts at: 0 143 206 1524 3015
Size: 143 63 1318 1491
Fragments arranged by size:
1491 1318 143 63
BsaBI GATnn'nnATC
Cuts at: 0 1906 2401 3015
Size: 1906 495 614
Fragments arranged by size:
1906 614 495
BsaHI Gr'CG_yC
Cuts at: 0 278 486 2053 3015
Size: 278 208 1567 962
Fragments arranged by size:
1567 962 278 208
BsaWI w'CCGG_w
Cuts at: 0 2750 3015
Size: 2750 265
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 739 769 877 907 991 1021 2545 2575
Size: 739 30 108 30 84 30 1524 30
Cuts at: 2575 3015
Size: 440
Fragments arranged by size:
1524 739 440 108 84 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 727 814 1750 1864 2240 2436 2569 2859
Size: 727 87 936 114 376 196 133 290
Cuts at: 2859 3015
Size: 156
Fragments arranged by size:
936 727 376 290 196 156 133 114
87
BseYI C'CCAG_C
Cuts at: 0 260 342 831 1160 2978 3015
Size: 260 82 489 329 1818 37
Fragments arranged by size:
1818 489 329 260 82 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 804 957 965 1198 2584 3015
Size: 804 153 8 233 1386 431
Fragments arranged by size:
1386 804 431 233 153 8
BsiEI CG_ry'CG
Cuts at: 0 50 449 3015
Size: 50 399 2566
Fragments arranged by size:
2566 399 50
BsmI GAATG_Cn'
Cuts at: 0 2960 3015
Size: 2960 55
BsmBI CGTCTCn'nnnn_
Cuts at: 0 266 493 1371 1500 3015
Size: 266 227 878 129 1515
Fragments arranged by size:
1515 878 266 227 129
Bsp1286I G_dGCh'C
Cuts at: 0 232 349 613 767 873 1047 1116 1160
Size: 232 117 264 154 106 174 69 44
Cuts at: 1160 1267 1274 1388 1516 1542 1820 1988 2342
Size: 107 7 114 128 26 278 168 354
Cuts at: 2342 2734 2979 3002 3015
Size: 392 245 23 13
Fragments arranged by size:
392 354 278 264 245 232 174 168
154 128 117 114 107 106 69 44
26 23 13 7
BspEI T'CCGG_A
Cuts at: 0 2750 3015
Size: 2750 265
BspHI T'CATG_A
Cuts at: 0 224 3015
Size: 224 2791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 76 358 1211 2717 3015
Size: 76 282 853 1506 298
Fragments arranged by size:
1506 853 298 282 76
BsrBI CCG'CTC
Cuts at: 0 717 804 1142 1347 2508 3015
Size: 717 87 338 205 1161 507
Fragments arranged by size:
1161 717 507 338 205 87
BsrDI GCAATG_nn'
Cuts at: 0 440 2132 3015
Size: 440 1692 883
Fragments arranged by size:
1692 883 440
BsrFI r'CCGG_y
Cuts at: 0 790 2011 2236 2834 3005 3015
Size: 790 1221 225 598 171 10
Fragments arranged by size:
1221 790 598 225 171 10
BssHII G'CGCG_C
Cuts at: 0 82 2910 3015
Size: 82 2828 105
Fragments arranged by size:
2828 105 82
BssSI C'ACGA_G
Cuts at: 0 255 760 3015
Size: 255 505 2255
Fragments arranged by size:
2255 505 255
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1191 1206 1584 2829 3015
Size: 1191 15 378 1245 186
Fragments arranged by size:
1245 1191 378 186 15
BstYI r'GATC_y
Cuts at: 0 1634 1901 2396 2948 3015
Size: 1634 267 495 552 67
Fragments arranged by size:
1634 552 495 267 67
Bsu36I CC'TnA_GG
Cuts at: 0 2173 3015
Size: 2173 842
BtgI C'CryG_G
Cuts at: 0 50 77 120 617 1577 1988 2112 2384
Size: 50 27 43 497 960 411 124 272
Cuts at: 2384 2579 2801 3015
Size: 195 222 214
Fragments arranged by size:
960 497 411 272 222 214 195 124
50 43 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2412 3015
Size: 2412 603
BtsI GCAGTG_nn'
Cuts at: 0 519 656 792 1077 3015
Size: 519 137 136 285 1938
Fragments arranged by size:
1938 519 285 137 136
EaeI y'GGCC_r
Cuts at: 0 47 62 446 575 979 1065 1196 1560
Size: 47 15 384 129 404 86 131 364
Cuts at: 1560 1991 2115 2282 2450 3015
Size: 431 124 167 168 565
Fragments arranged by size:
565 431 404 384 364 168 167 131
129 124 86 47 15
EagI C'GGCC_G
Cuts at: 0 47 446 3015
Size: 47 399 2569
Fragments arranged by size:
2569 399 47
EarI CTCTTCn'nnn_
Cuts at: 0 1687 3015
Size: 1687 1328
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2080 2876 3015
Size: 2080 796 139
Fragments arranged by size:
2080 796 139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 887 993 1057 1432 1551 1949 2374
Size: 400 487 106 64 375 119 398 425
Cuts at: 2374 3015
Size: 641
Fragments arranged by size:
641 487 425 400 398 375 119 106
64
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1177 2047 2172 3015
Size: 1177 870 125 843
Fragments arranged by size:
1177 870 843 125
EcoO109I rG'GnC_Cy
Cuts at: 0 264 520 1044 2001 2177 2793 2998 2999
Size: 264 256 524 957 176 616 205 1
Cuts at: 2999 3015
Size: 16
Fragments arranged by size:
957 616 524 264 256 205 176 16
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 378 555 3015
Size: 378 177 2460
Fragments arranged by size:
2460 378 177
FspI TGC'GCA
Cuts at: 0 253 511 625 3015
Size: 253 258 114 2390
Fragments arranged by size:
2390 258 253 114
HaeII r_GCGC'y
Cuts at: 0 281 3015
Size: 281 2734
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 655 687 946 978 1478 1510 1734 1766
Size: 655 32 259 32 500 32 224 32
Cuts at: 1766 3015
Size: 1249
Fragments arranged by size:
1249 655 500 259 224 32 32 32
32
HincII GTy'rAC
Cuts at: 0 113 1293 2304 3015
Size: 113 1180 1011 711
Fragments arranged by size:
1180 1011 711 113
KasI G'GCGC_C
Cuts at: 0 277 3015
Size: 277 2738
KpnI G_GTAC'C
Cuts at: 0 2565 2841 3015
Size: 2565 276 174
Fragments arranged by size:
2565 276 174
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 1280 3015
Size: 365 915 1735
Fragments arranged by size:
1735 915 365
MscI TGG'CCA
Cuts at: 0 577 1198 1993 3015
Size: 577 621 795 1022
Fragments arranged by size:
1022 795 621 577
MslI CAynn'nnrTG
Cuts at: 0 70 505 1582 2200 2827 2929 3015
Size: 70 435 1077 618 627 102 86
Fragments arranged by size:
1077 627 618 435 102 86 70
MspA1I CmG'CkG
Cuts at: 0 52 79 864 875 1103 1124 1285 1977
Size: 52 27 785 11 228 21 161 692
Cuts at: 1977 2208 2408 2643 2780 2982 3015
Size: 231 200 235 137 202 33
Fragments arranged by size:
785 692 235 231 228 202 200 161
137 52 33 27 21 11
NaeI GCC'GGC
Cuts at: 0 792 2013 2238 3007 3015
Size: 792 1221 225 769 8
Fragments arranged by size:
1221 792 769 225 8
NarI GG'CG_CC
Cuts at: 0 278 3015
Size: 278 2737
NcoI C'CATG_G
Cuts at: 0 617 1577 2579 3015
Size: 617 960 1002 436
Fragments arranged by size:
1002 960 617 436
NgoMIV G'CCGG_C
Cuts at: 0 790 2011 2236 3005 3015
Size: 790 1221 225 769 10
Fragments arranged by size:
1221 790 769 225 10
NspI r_CATG'y
Cuts at: 0 333 1136 2220 2244 3015
Size: 333 803 1084 24 771
Fragments arranged by size:
1084 803 771 333 24
PasI CC'CwG_GG
Cuts at: 0 853 1109 2916 2994 3015
Size: 853 256 1807 78 21
Fragments arranged by size:
1807 853 256 78 21
PflMI CCAn_nnn'nTGG
Cuts at: 0 1329 1578 1631 2408 3015
Size: 1329 249 53 777 607
Fragments arranged by size:
1329 777 607 249 53
PfoI T'CCnGG_A
Cuts at: 0 554 1409 1721 3015
Size: 554 855 312 1294
Fragments arranged by size:
1294 855 554 312
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 991 1023 1807 1839 3015
Size: 991 32 784 32 1176
Fragments arranged by size:
1176 991 784 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 264 520 2177 3015
Size: 264 256 1657 838
Fragments arranged by size:
1657 838 264 256
PshAI GACnn'nnGTC
Cuts at: 0 1363 1627 3015
Size: 1363 264 1388
Fragments arranged by size:
1388 1363 264
PspOMI G'GGCC_C
Cuts at: 0 1043 2975 2998 3015
Size: 1043 1932 23 17
Fragments arranged by size:
1932 1043 23 17
PspXI vC'TCGA_Gb
Cuts at: 0 2095 3015
Size: 2095 920
PstI C_TGCA'G
Cuts at: 0 742 3015
Size: 742 2273
PvuII CAG'CTG
Cuts at: 0 864 2208 2982 3015
Size: 864 1344 774 33
Fragments arranged by size:
1344 864 774 33
RsrII CG'GwC_CG
Cuts at: 0 387 3015
Size: 387 2628
SacII CC_GC'GG
Cuts at: 0 53 80 3015
Size: 53 27 2935
Fragments arranged by size:
2935 53 27
SalI G'TCGA_C
Cuts at: 0 111 3015
Size: 111 2904
SanDI GG'GwC_CC
Cuts at: 0 264 520 3015
Size: 264 256 2495
Fragments arranged by size:
2495 264 256
ScaI AGT'ACT
Cuts at: 0 1429 3015
Size: 1429 1586
SexAI A'CCwGG_T
Cuts at: 0 209 2312 3015
Size: 209 2103 703
Fragments arranged by size:
2103 703 209
SfcI C'TryA_G
Cuts at: 0 738 1707 3015
Size: 738 969 1308
Fragments arranged by size:
1308 969 738
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1865 1999 2422 3015
Size: 1865 134 423 593
Fragments arranged by size:
1865 593 423 134
SfoI GGC'GCC
Cuts at: 0 279 3015
Size: 279 2736
SmaI CCC'GGG
Cuts at: 0 13 1802 3015
Size: 13 1789 1213
Fragments arranged by size:
1789 1213 13
SmlI C'TyrA_G
Cuts at: 0 490 1468 1504 2095 3015
Size: 490 978 36 591 920
Fragments arranged by size:
978 920 591 490 36
SphI G_CATG'C
Cuts at: 0 333 2220 2244 3015
Size: 333 1887 24 771
Fragments arranged by size:
1887 771 333 24
StuI AGG'CCT
Cuts at: 0 1717 1894 3015
Size: 1717 177 1121
Fragments arranged by size:
1717 1121 177
StyI C'CwwG_G
Cuts at: 0 474 617 1577 2579 3015
Size: 474 143 960 1002 436
Fragments arranged by size:
1002 960 474 436 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 146 741 2221 2592 3007 3015
Size: 146 595 1480 371 415 8
Fragments arranged by size:
1480 595 415 371 146 8
TatI w'GTAC_w
Cuts at: 0 734 1427 2141 3015
Size: 734 693 714 874
Fragments arranged by size:
874 734 714 693
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 739 771 850 877 882 909 1763 1795
Size: 739 32 79 27 5 27 854 32
Cuts at: 1795 3015
Size: 1220
Fragments arranged by size:
1220 854 739 79 32 32 27 27
5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 174 1191 2205 3015
Size: 174 1017 1014 810
Fragments arranged by size:
1017 1014 810 174
XhoI C'TCGA_G
Cuts at: 0 2095 3015
Size: 2095 920
XmaI C'CCGG_G
Cuts at: 0 11 1800 3015
Size: 11 1789 1215
Fragments arranged by size:
1789 1215 11
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuII RsrII SacII SalI SanDI ScaI SexAI SfcI
SfiI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI ApaLI AscI AseI
AsiSI AvrII BaeI BclI BfrBI BglII BlpI BmtI
BsiHKAI BsiWI BsrGI BstAPI BstBI BstEII BstZ17I ClaI
CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FseI
FspAI HindIII HpaI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PsiI PsrI
PvuI SacI SapI SbfI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XmnI ZraI