(Circular) (Six-base) MAPSORT of: pFN21AB7163.seq Check: 4927 from: 1 to: 5869 pFN21AB7163 5869 bp With 182 enzymes: SgfI * October 20, 2009 10:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3679 278 Size: 53 83 186 936 2143 2468 Fragments arranged by size: 2468 2143 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2876 3212 1937 Size: 939 336 4594 Fragments arranged by size: 4594 939 336 Acc65I G'GTAC_C Cuts at: 3190 3190 Size: 5869 AclI AA'CG_TT Cuts at: 1563 3996 4369 1563 Size: 2433 373 3063 Fragments arranged by size: 3063 2433 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2080 2521 2600 2705 2743 2901 3050 3932 Size: 448 441 79 105 38 158 149 882 Cuts at: 3932 5316 5584 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 882 448 441 268 158 149 105 79 38 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5672 AflIII A'CryG_T Cuts at: 1203 4768 5745 1203 Size: 3565 977 1327 Fragments arranged by size: 3565 1327 977 AgeI A'CCGG_T Cuts at: 2618 4700 2618 Size: 2082 3787 AhdI GACnn_n'nnGTC Cuts at: 4598 4598 Size: 5869 AleI CACnn'nnGTG Cuts at: 1166 2621 1166 Size: 1455 4414 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5869 AlwNI CAG_nnn'CTG Cuts at: 3612 5184 3612 Size: 1572 4297 ApaLI G'TGCA_C Cuts at: 3926 5082 3926 Size: 1156 4713 ApoI r'AATT_y Cuts at: 1493 2318 2783 3178 3404 3556 5665 1493 Size: 825 465 395 226 152 2109 1697 Fragments arranged by size: 2109 1697 825 465 395 226 152 AseI AT'TA_AT Cuts at: 160 1032 4423 160 Size: 872 3391 1606 Fragments arranged by size: 3391 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 AvaI C'yCGr_G Cuts at: 1870 1943 3194 1870 Size: 73 1251 4545 Fragments arranged by size: 4545 1251 73 BamHI G'GATC_C Cuts at: 3199 3566 3199 Size: 367 5502 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3109 3190 4645 618 Size: 325 217 627 1322 81 1455 1842 Fragments arranged by size: 1842 1455 1322 627 325 217 81 BanII G_rGCy'C Cuts at: 1391 2037 2151 2208 3188 1391 Size: 646 114 57 980 4072 Fragments arranged by size: 4072 980 646 114 57 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2693 928 Size: 971 794 4104 Fragments arranged by size: 4104 971 794 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4060 4094 1903 Size: 34 2123 34 3678 Fragments arranged by size: 3678 2123 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3761 4971 3761 Size: 1210 4659 BclI T'GATC_A Cuts at: 1548 1548 Size: 5869 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4480 136 Size: 107 122 71 631 605 2808 1525 Fragments arranged by size: 2808 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5864 5864 Size: 5869 BlpI GC'TnA_GC Cuts at: 3286 3286 Size: 5869 Bme1580I G_kGCm'C Cuts at: 1790 2112 3930 5086 1790 Size: 322 1818 1156 2573 Fragments arranged by size: 2573 1818 1156 322 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5869 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2106 2513 2861 Size: 416 53 83 399 24 689 407 348 Cuts at: 2861 2914 3600 4558 442 Size: 53 686 958 1753 Fragments arranged by size: 1753 958 689 686 416 407 399 348 83 53 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5869 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2366 2366 Size: 5869 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 2678 2696 4529 1239 Size: 896 543 18 1833 2579 Fragments arranged by size: 2579 1833 896 543 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2000 3994 4859 5157 2000 Size: 1994 865 298 2712 Fragments arranged by size: 2712 1994 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2096 4532 882 Size: 617 597 2436 2219 Fragments arranged by size: 2436 2219 617 597 BsaAI yAC'GTr Cuts at: 493 1204 3660 493 Size: 711 2456 2702 Fragments arranged by size: 2702 2456 711 BsaBI GATnn'nnATC Cuts at: 5863 5863 Size: 5869 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3676 4058 4693 275 Size: 53 83 186 936 2143 382 635 1451 Fragments arranged by size: 2143 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2618 4302 4700 4974 5121 5532 2618 Size: 1684 398 274 147 411 2955 Fragments arranged by size: 2955 1684 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2550 2580 1178 Size: 30 165 30 1147 30 4467 Fragments arranged by size: 4467 1147 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5869 BseYI C'CCAG_C Cuts at: 5072 5568 5072 Size: 496 5373 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2547 2955 1163 Size: 573 811 408 4077 Fragments arranged by size: 4077 811 573 408 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2997 3319 4080 5108 1540 Size: 275 186 996 322 761 1028 2301 Fragments arranged by size: 2301 1028 996 761 322 275 186 BsiHKAI G_wGCw'C Cuts at: 2037 2151 3930 4015 5086 2037 Size: 114 1779 85 1071 2820 Fragments arranged by size: 2820 1779 1071 114 85 BsmI GAATG_Cn' Cuts at: 3380 3473 3380 Size: 93 5776 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2112 2151 2208 3188 3930 4015 Size: 399 247 75 39 57 980 742 85 Cuts at: 4015 5086 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 980 742 399 247 85 75 57 39 BspEI T'CCGG_A Cuts at: 5532 5532 Size: 5869 BspHI T'CATG_A Cuts at: 3758 5488 3758 Size: 1730 4139 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3224 844 Size: 2380 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2561 3756 1394 Size: 429 738 1195 3507 Fragments arranged by size: 3507 1195 738 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4364 4538 65 Size: 1183 3116 174 1396 Fragments arranged by size: 3116 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2618 4513 4700 1700 Size: 110 808 1895 187 2869 Fragments arranged by size: 2869 1895 808 187 110 BsrGI T'GTAC_A Cuts at: 96 2841 96 Size: 2745 3124 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5869 BssSI C'ACGA_G Cuts at: 2046 3929 4941 2046 Size: 1883 1012 2974 Fragments arranged by size: 2974 1883 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5869 BstBI TT'CG_AA Cuts at: 4673 4673 Size: 5869 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5869 BstXI CCAn_nnnn'nTGG Cuts at: 2623 2656 3092 2623 Size: 33 436 5400 Fragments arranged by size: 5400 436 33 BstYI r'GATC_y Cuts at: 1972 2019 2884 3199 3566 3951 3968 5420 5506 Size: 47 865 315 367 385 17 1452 86 Cuts at: 5506 5518 5864 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 865 385 367 346 315 86 47 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5317 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2389 487 Size: 683 56 1163 3967 Fragments arranged by size: 3967 1163 683 56 BtsI GCAGTG_nn' Cuts at: 3385 4179 4199 3385 Size: 794 20 5055 Fragments arranged by size: 5055 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5869 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5834 DraI TTT'AAA Cuts at: 3173 3173 Size: 5869 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4876 809 Size: 507 3560 1802 Fragments arranged by size: 3560 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2493 2928 2994 Size: 54 1425 50 275 18 663 435 66 Cuts at: 2994 3316 4205 5834 8 Size: 322 889 1629 43 Fragments arranged by size: 1629 1425 889 663 435 322 275 66 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2994 3316 1537 Size: 275 1182 322 4090 Fragments arranged by size: 4090 1182 322 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2081 2099 3799 1353 Size: 728 18 1700 3423 Fragments arranged by size: 3423 1700 728 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4456 4830 4976 387 Size: 1064 138 2867 374 146 1280 Fragments arranged by size: 2867 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2035 2149 2035 Size: 114 5755 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2080 2135 2521 2600 2678 2696 2705 Size: 393 448 55 386 79 78 18 9 Cuts at: 2705 2743 2901 3050 3932 4529 5316 5584 1239 Size: 38 158 149 882 597 787 268 1524 Fragments arranged by size: 1524 882 787 597 448 393 386 268 158 149 79 78 55 38 18 9 EcoO109I rG'GnC_Cy Cuts at: 2140 2140 Size: 5869 EcoRI G'AATT_C Cuts at: 2783 3178 3556 2783 Size: 395 378 5096 Fragments arranged by size: 5096 395 378 FspI TGC'GCA Cuts at: 4375 4375 Size: 5869 HaeII r_GCGC'y Cuts at: 1678 5016 1678 Size: 3338 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4510 4542 4584 4616 1373 Size: 32 135 32 2938 32 42 32 2626 Fragments arranged by size: 2938 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3213 3459 669 Size: 988 281 1275 246 3079 Fragments arranged by size: 3079 1275 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5869 HpaI GTT'AAC Cuts at: 3459 3459 Size: 5869 KpnI G_GTAC'C Cuts at: 3194 3194 Size: 5869 MfeI C'AATT_G Cuts at: 3468 3468 Size: 5869 MluI A'CGCG_T Cuts at: 5745 5745 Size: 5869 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4983 5167 1305 Size: 3678 184 2007 Fragments arranged by size: 3678 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2495 10 Size: 54 1425 343 663 3384 Fragments arranged by size: 3384 1425 663 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2621 3827 4186 4345 518 Size: 648 1455 1206 359 159 2042 Fragments arranged by size: 2042 1455 1206 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3285 3962 5110 5355 1606 Size: 1679 677 1148 245 2120 Fragments arranged by size: 2120 1679 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5317 NdeI CA'TA_TG Cuts at: 387 387 Size: 5869 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5869 NotI GC'GGCC_GC Cuts at: 3316 3316 Size: 5869 NspI r_CATG'y Cuts at: 1132 1229 3227 4772 1132 Size: 97 1998 1545 2229 Fragments arranged by size: 2229 1998 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5869 PciI A'CATG_T Cuts at: 4768 4768 Size: 5869 PflMI CCAn_nnn'nTGG Cuts at: 1108 2950 3096 1108 Size: 1842 146 3881 Fragments arranged by size: 3881 1842 146 PfoI T'CCnGG_A Cuts at: 2151 2655 2151 Size: 504 5365 PmeI GTTT'AAAC Cuts at: 3173 3173 Size: 5869 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3938 3970 5451 5483 3938 Size: 32 1481 32 4324 Fragments arranged by size: 4324 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2140 2140 Size: 5869 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5869 PsiI TTA'TAA Cuts at: 3439 3439 Size: 5869 PstI C_TGCA'G Cuts at: 830 3221 830 Size: 2391 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5869 SacI G_AGCT'C Cuts at: 2037 2151 2037 Size: 114 5755 SalI G'TCGA_C Cuts at: 1936 3211 1936 Size: 1275 4594 SbfI CC_TGCA'GG Cuts at: 3221 3221 Size: 5869 ScaI AGT'ACT Cuts at: 4117 5829 4117 Size: 1712 4157 SexAI A'CCwGG_T Cuts at: 2807 3153 2807 Size: 346 5523 SfcI C'TryA_G Cuts at: 826 1045 3217 4352 5033 5224 826 Size: 219 2172 1135 681 191 1471 Fragments arranged by size: 2172 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5869 SgrAI Cr'CCGG_yG Cuts at: 1700 2618 1700 Size: 918 4951 SmaI CCC'GGG Cuts at: 1872 3196 1872 Size: 1324 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2015 3973 4874 5136 820 Size: 197 926 72 1958 901 262 1553 Fragments arranged by size: 1958 1553 926 901 262 197 72 SnaBI TAC'GTA Cuts at: 493 3660 493 Size: 3167 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5869 SphI G_CATG'C Cuts at: 1132 3227 1132 Size: 2095 3774 SspI AAT'ATT Cuts at: 5 52 3793 5 Size: 47 3741 2081 Fragments arranged by size: 3741 2081 47 StuI AGG'CCT Cuts at: 1517 2673 2747 1517 Size: 1156 74 4639 Fragments arranged by size: 4639 1156 74 StyI C'CwwG_G Cuts at: 513 1065 3308 513 Size: 552 2243 3074 Fragments arranged by size: 3074 2243 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3896 3913 4066 4251 4696 325 Size: 1255 2316 17 153 185 445 1498 Fragments arranged by size: 2316 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2841 4115 5827 Size: 274 80 33 51 1443 864 1274 1712 Cuts at: 5827 96 Size: 138 Fragments arranged by size: 1712 1443 1274 864 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5829 5861 5829 Size: 32 5837 Tth111I GACn'n_nGTC Cuts at: 1316 3058 1316 Size: 1742 4127 XbaI T'CTAG_A Cuts at: 3205 3205 Size: 5869 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5869 XmaI C'CCGG_G Cuts at: 1870 3194 1870 Size: 1324 4545 XmnI GAAnn'nnTTC Cuts at: 3998 3998 Size: 5869 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3677 276 Size: 53 83 186 936 2143 2468 Fragments arranged by size: 2468 2143 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI