(Linear) (Six-base) MAPSORT of: KIEE9259.seq Check: 5966 from: 1 to: 3630 KIEE9259.orf With 182 enzymes: SgfI * April 20, 2012 13:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2266 2918 3508 3630 Size: 2266 652 590 122 Fragments arranged by size: 2266 652 590 122 AatII G_ACGT'C Cuts at: 0 2333 2432 2878 3630 Size: 2333 99 446 752 Fragments arranged by size: 2333 752 446 99 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 792 887 1643 3399 3630 Size: 792 95 756 1756 231 Fragments arranged by size: 1756 792 756 231 95 AfeI AGC'GCT Cuts at: 0 2680 3630 Size: 2680 950 AflIII A'CryG_T Cuts at: 0 2687 2923 3630 Size: 2687 236 707 Fragments arranged by size: 2687 707 236 AhdI GACnn_n'nnGTC Cuts at: 0 768 861 3630 Size: 768 93 2769 Fragments arranged by size: 2769 768 93 AleI CACnn'nnGTG Cuts at: 0 2926 3341 3630 Size: 2926 415 289 Fragments arranged by size: 2926 415 289 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2080 3127 3630 Size: 2080 1047 503 Fragments arranged by size: 2080 1047 503 AlwNI CAG_nnn'CTG Cuts at: 0 205 493 1687 3630 Size: 205 288 1194 1943 Fragments arranged by size: 1943 1194 288 205 ApaI G_GGCC'C Cuts at: 0 1823 3630 Size: 1823 1807 ApoI r'AATT_y Cuts at: 0 3013 3630 Size: 3013 617 AvaI C'yCGr_G Cuts at: 0 766 1482 1646 1717 2576 2674 2780 3422 Size: 766 716 164 71 859 98 106 642 Cuts at: 3422 3598 3630 Size: 176 32 Fragments arranged by size: 859 766 716 642 176 164 106 98 71 32 BamHI G'GATC_C Cuts at: 0 317 3630 Size: 317 3313 BanI G'GyrC_C Cuts at: 0 1293 1510 1775 2422 3630 Size: 1293 217 265 647 1208 Fragments arranged by size: 1293 1208 647 265 217 BanII G_rGCy'C Cuts at: 0 471 520 549 1132 1823 2089 2834 3605 Size: 471 49 29 583 691 266 745 771 Cuts at: 3605 3630 Size: 25 Fragments arranged by size: 771 745 691 583 471 266 49 29 25 BbeI G_GCGC'C Cuts at: 0 1779 2426 3630 Size: 1779 647 1204 Fragments arranged by size: 1779 1204 647 BbsI GAAGACnn'nnnn_ Cuts at: 0 1584 3363 3630 Size: 1584 1779 267 Fragments arranged by size: 1779 1584 267 BbvCI CC'TCA_GC Cuts at: 0 3224 3630 Size: 3224 406 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3526 3560 3630 Size: 3526 34 70 Fragments arranged by size: 3526 70 34 BglI GCCn_nnn'nGGC Cuts at: 0 1875 2960 3509 3630 Size: 1875 1085 549 121 Fragments arranged by size: 1875 1085 549 121 BglII A'GATC_T Cuts at: 0 666 2096 3630 Size: 666 1430 1534 Fragments arranged by size: 1534 1430 666 BlpI GC'TnA_GC Cuts at: 0 2311 2968 3630 Size: 2311 657 662 Fragments arranged by size: 2311 662 657 Bme1580I G_kGCm'C Cuts at: 0 1331 1513 1749 1823 2801 3630 Size: 1331 182 236 74 978 829 Fragments arranged by size: 1331 978 829 236 182 74 BmgBI CAC'GTC Cuts at: 0 2688 2775 3630 Size: 2688 87 855 Fragments arranged by size: 2688 855 87 BmrI ACTGGGnnnn_n' Cuts at: 0 658 1136 1736 1882 2478 3446 3630 Size: 658 478 600 146 596 968 184 Fragments arranged by size: 968 658 600 596 478 184 146 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3231 3624 3630 Size: 3231 393 6 Fragments arranged by size: 3231 393 6 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 182 196 488 796 1195 1261 1540 Size: 97 85 14 292 308 399 66 279 Cuts at: 1540 2141 2212 2992 3090 3571 3630 Size: 601 71 780 98 481 59 Fragments arranged by size: 780 601 481 399 308 292 279 98 97 85 71 66 59 14 Bpu10I CC'TnA_GC Cuts at: 0 1815 2630 3224 3587 3630 Size: 1815 815 594 363 43 Fragments arranged by size: 1815 815 594 363 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 1375 1613 3630 Size: 307 1068 238 2017 Fragments arranged by size: 2017 1068 307 238 BsaI GGTCTCn'nnnn_ Cuts at: 0 1393 1438 3630 Size: 1393 45 2192 Fragments arranged by size: 2192 1393 45 BsaAI yAC'GTr Cuts at: 0 2617 2924 3630 Size: 2617 307 706 Fragments arranged by size: 2617 706 307 BsaHI Gr'CG_yC Cuts at: 0 1776 2158 2330 2359 2423 2429 2875 3630 Size: 1776 382 172 29 64 6 446 755 Fragments arranged by size: 1776 755 446 382 172 64 29 6 BsaWI w'CCGG_w Cuts at: 0 2975 3431 3630 Size: 2975 456 199 Fragments arranged by size: 2975 456 199 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1668 1698 2458 2488 3436 3466 3630 Size: 1668 30 760 30 948 30 164 Fragments arranged by size: 1668 948 760 164 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 107 727 793 916 919 922 931 955 Size: 107 620 66 123 3 3 9 24 Cuts at: 955 958 988 1122 1127 1276 2257 2464 3457 Size: 3 30 134 5 149 981 207 993 Cuts at: 3457 3630 Size: 173 Fragments arranged by size: 993 981 620 207 173 149 134 123 107 66 30 24 9 5 3 3 3 BseYI C'CCAG_C Cuts at: 0 324 358 1183 1213 3146 3630 Size: 324 34 825 30 1933 484 Fragments arranged by size: 1933 825 484 324 34 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1296 1485 1673 2233 2449 2548 2904 3630 Size: 1296 189 188 560 216 99 356 726 Fragments arranged by size: 1296 726 560 356 216 189 188 99 BsiEI CG_ry'CG Cuts at: 0 35 1673 2895 3468 3630 Size: 35 1638 1222 573 162 Fragments arranged by size: 1638 1222 573 162 35 BsiHKAI G_wGCw'C Cuts at: 0 1439 2317 2384 3064 3605 3630 Size: 1439 878 67 680 541 25 Fragments arranged by size: 1439 878 680 541 67 25 BsmI GAATG_Cn' Cuts at: 0 2520 3630 Size: 2520 1110 BsmBI CGTCTCn'nnnn_ Cuts at: 0 781 1070 2866 3630 Size: 781 289 1796 764 Fragments arranged by size: 1796 781 764 289 Bsp1286I G_dGCh'C Cuts at: 0 471 520 549 1132 1331 1439 1513 1749 Size: 471 49 29 583 199 108 74 236 Cuts at: 1749 1823 2089 2317 2384 2801 2834 3064 3605 Size: 74 266 228 67 417 33 230 541 Cuts at: 3605 3630 Size: 25 Fragments arranged by size: 583 541 471 417 266 236 230 228 199 108 74 74 67 49 33 29 25 BspEI T'CCGG_A Cuts at: 0 3431 3630 Size: 3431 199 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2266 2918 3398 3508 3630 Size: 2266 652 480 110 122 Fragments arranged by size: 2266 652 480 122 110 BsrDI GCAATG_nn' Cuts at: 0 1383 3542 3630 Size: 1383 2159 88 Fragments arranged by size: 2159 1383 88 BsrFI r'CCGG_y Cuts at: 0 1013 1668 1672 3630 Size: 1013 655 4 1958 Fragments arranged by size: 1958 1013 655 4 BssHII G'CGCG_C Cuts at: 0 2518 3630 Size: 2518 1112 BssSI C'ACGA_G Cuts at: 0 513 1109 3630 Size: 513 596 2521 Fragments arranged by size: 2521 596 513 BstAPI GCAn_nnn'nTGC Cuts at: 0 1687 2978 3630 Size: 1687 1291 652 Fragments arranged by size: 1687 1291 652 BstEII G'GTnAC_C Cuts at: 0 657 1635 1659 3630 Size: 657 978 24 1971 Fragments arranged by size: 1971 978 657 24 BstXI CCAn_nnnn'nTGG Cuts at: 0 1422 2347 3549 3630 Size: 1422 925 1202 81 Fragments arranged by size: 1422 1202 925 81 BstYI r'GATC_y Cuts at: 0 317 666 2096 3630 Size: 317 349 1430 1534 Fragments arranged by size: 1534 1430 349 317 Bsu36I CC'TnA_GG Cuts at: 0 1134 2840 3630 Size: 1134 1706 790 Fragments arranged by size: 1706 1134 790 BtgI C'CryG_G Cuts at: 0 306 1051 1093 1859 2090 2174 2547 3630 Size: 306 745 42 766 231 84 373 1083 Fragments arranged by size: 1083 766 745 373 306 231 84 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2481 2553 3390 3630 Size: 2481 72 837 240 Fragments arranged by size: 2481 837 240 72 BtsI GCAGTG_nn' Cuts at: 0 1473 1676 2144 2916 3630 Size: 1473 203 468 772 714 Fragments arranged by size: 1473 772 714 468 203 ClaI AT'CG_AT Cuts at: 0 2891 3630 Size: 2891 739 EaeI y'GGCC_r Cuts at: 0 32 671 1011 1340 1670 2961 3630 Size: 32 639 340 329 330 1291 669 Fragments arranged by size: 1291 669 639 340 330 329 32 EagI C'GGCC_G Cuts at: 0 32 1670 3630 Size: 32 1638 1960 Fragments arranged by size: 1960 1638 32 EarI CTCTTCn'nnn_ Cuts at: 0 357 893 908 926 932 944 956 3262 Size: 357 536 15 18 6 12 12 2306 Cuts at: 3262 3630 Size: 368 Fragments arranged by size: 2306 536 368 357 18 15 12 12 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1944 3630 Size: 1944 1686 EcoICRI GAG'CTC Cuts at: 0 3603 3630 Size: 3603 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 182 196 488 792 796 887 1195 Size: 97 85 14 292 304 4 91 308 Cuts at: 1195 1261 1540 1643 2141 2212 2992 3090 3399 Size: 66 279 103 498 71 780 98 309 Cuts at: 3399 3571 3630 Size: 172 59 Fragments arranged by size: 780 498 309 308 304 292 279 172 103 98 97 91 85 71 66 59 14 4 EcoNI CCTnn'n_nnAGG Cuts at: 0 154 709 1705 1897 3630 Size: 154 555 996 192 1733 Fragments arranged by size: 1733 996 555 192 154 EcoO109I rG'GnC_Cy Cuts at: 0 48 300 762 770 1138 1158 1169 1193 Size: 48 252 462 8 368 20 11 24 Cuts at: 1193 1819 1820 1890 1902 2664 3182 3630 Size: 626 1 70 12 762 518 448 Fragments arranged by size: 762 626 518 462 448 368 252 70 48 24 20 12 11 8 1 HaeII r_GCGC'y Cuts at: 0 70 1779 2426 2492 2682 3630 Size: 70 1709 647 66 190 948 Fragments arranged by size: 1709 948 647 190 70 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 628 651 660 683 754 786 814 846 Size: 628 23 9 23 71 32 28 32 Cuts at: 846 847 879 961 993 2923 2955 3015 3047 Size: 1 32 82 32 1930 32 60 32 Cuts at: 3047 3630 Size: 583 Fragments arranged by size: 1930 628 583 82 71 60 32 32 32 32 32 32 28 23 23 9 1 HincII GTy'rAC Cuts at: 0 2334 2541 2814 3624 3630 Size: 2334 207 273 810 6 Fragments arranged by size: 2334 810 273 207 6 KasI G'GCGC_C Cuts at: 0 1775 2422 3630 Size: 1775 647 1208 Fragments arranged by size: 1775 1208 647 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 689 878 1991 2708 2745 2972 3630 Size: 689 189 1113 717 37 227 658 Fragments arranged by size: 1113 717 689 658 227 189 37 MscI TGG'CCA Cuts at: 0 673 1342 3630 Size: 673 669 2288 Fragments arranged by size: 2288 673 669 MslI CAynn'nnrTG Cuts at: 0 2395 2926 3109 3341 3630 Size: 2395 531 183 232 289 Fragments arranged by size: 2395 531 289 232 183 MspA1I CmG'CkG Cuts at: 0 25 55 1095 1280 1578 2154 3036 3630 Size: 25 30 1040 185 298 576 882 594 Fragments arranged by size: 1040 882 594 576 298 185 30 25 NaeI GCC'GGC Cuts at: 0 1015 1674 3630 Size: 1015 659 1956 Fragments arranged by size: 1956 1015 659 NarI GG'CG_CC Cuts at: 0 1776 2423 3630 Size: 1776 647 1207 Fragments arranged by size: 1776 1207 647 NcoI C'CATG_G Cuts at: 0 1051 2547 3630 Size: 1051 1496 1083 Fragments arranged by size: 1496 1083 1051 NgoMIV G'CCGG_C Cuts at: 0 1013 1672 3630 Size: 1013 659 1958 Fragments arranged by size: 1958 1013 659 NotI GC'GGCC_GC Cuts at: 0 32 3630 Size: 32 3598 NspI r_CATG'y Cuts at: 0 2071 2409 3630 Size: 2071 338 1221 Fragments arranged by size: 2071 1221 338 PasI CC'CwG_GG Cuts at: 0 471 3630 Size: 471 3159 PflMI CCAn_nnn'nTGG Cuts at: 0 265 438 574 648 2369 3630 Size: 265 173 136 74 1721 1261 Fragments arranged by size: 1721 1261 265 173 136 74 PfoI T'CCnGG_A Cuts at: 0 1517 1913 3008 3630 Size: 1517 396 1095 622 Fragments arranged by size: 1517 1095 622 396 PmlI CAC'GTG Cuts at: 0 2617 2924 3630 Size: 2617 307 706 Fragments arranged by size: 2617 706 307 PpuMI rG'GwC_Cy Cuts at: 0 300 762 770 1158 1169 1193 2664 3182 Size: 300 462 8 388 11 24 1471 518 Cuts at: 3182 3630 Size: 448 Fragments arranged by size: 1471 518 462 448 388 300 24 11 8 PshAI GACnn'nnGTC Cuts at: 0 809 3630 Size: 809 2821 PspOMI G'GGCC_C Cuts at: 0 1819 3630 Size: 1819 1811 PstI C_TGCA'G Cuts at: 0 24 483 1683 1991 2117 2379 2915 3167 Size: 24 459 1200 308 126 262 536 252 Cuts at: 3167 3630 Size: 463 Fragments arranged by size: 1200 536 463 459 308 262 252 126 24 PvuI CG_AT'CG Cuts at: 0 2895 3630 Size: 2895 735 PvuII CAG'CTG Cuts at: 0 1280 1578 3630 Size: 1280 298 2052 Fragments arranged by size: 2052 1280 298 SacI G_AGCT'C Cuts at: 0 3605 3630 Size: 3605 25 SacII CC_GC'GG Cuts at: 0 1096 3630 Size: 1096 2534 SanDI GG'GwC_CC Cuts at: 0 762 770 1169 1193 3630 Size: 762 8 399 24 2437 Fragments arranged by size: 2437 762 399 24 8 SfcI C'TryA_G Cuts at: 0 20 245 479 1679 1987 2113 2232 2375 Size: 20 225 234 1200 308 126 119 143 Cuts at: 2375 2911 3118 3163 3630 Size: 536 207 45 467 Fragments arranged by size: 1200 536 467 308 234 225 207 143 126 119 45 20 SfoI GGC'GCC Cuts at: 0 1777 2424 3630 Size: 1777 647 1206 Fragments arranged by size: 1777 1206 647 SmaI CCC'GGG Cuts at: 0 768 1484 1648 1719 3630 Size: 768 716 164 71 1911 Fragments arranged by size: 1911 768 716 164 71 SmlI C'TyrA_G Cuts at: 0 286 1390 1628 3630 Size: 286 1104 238 2002 Fragments arranged by size: 2002 1104 286 238 SphI G_CATG'C Cuts at: 0 2071 2409 3630 Size: 2071 338 1221 Fragments arranged by size: 2071 1221 338 SrfI GCCC'GGGC Cuts at: 0 1484 3630 Size: 1484 2146 SspI AAT'ATT Cuts at: 0 3493 3630 Size: 3493 137 StuI AGG'CCT Cuts at: 0 1564 3630 Size: 1564 2066 StyI C'CwwG_G Cuts at: 0 155 533 1051 1100 2547 3630 Size: 155 378 518 49 1447 1083 Fragments arranged by size: 1447 1083 518 378 155 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 787 1044 1311 2920 3630 Size: 787 257 267 1609 710 Fragments arranged by size: 1609 787 710 267 257 TatI w'GTAC_w Cuts at: 0 1148 2852 3287 3630 Size: 1148 1704 435 343 Fragments arranged by size: 1704 1148 435 343 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1668 1700 2458 2490 3630 Size: 1668 32 758 32 1140 Fragments arranged by size: 1668 1140 758 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1149 1350 3630 Size: 1149 201 2280 Fragments arranged by size: 2280 1149 201 XmaI C'CCGG_G Cuts at: 0 766 1482 1646 1717 3630 Size: 766 716 164 71 1913 Fragments arranged by size: 1913 766 716 164 71 XmnI GAAnn'nnTTC Cuts at: 0 2507 3630 Size: 2507 1123 ZraI GAC'GTC Cuts at: 0 2331 2430 2876 3630 Size: 2331 99 446 754 Fragments arranged by size: 2331 754 446 99 Enzymes that do cut: AarI AatII AcuI AfeI AflIII AhdI AleI AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuI PvuII SacI SacII SanDI SfcI SfoI SmaI SmlI SphI SrfI SspI StuI StyI TaqII TatI TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AgeI AloI ApaLI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BmtI BsaBI BsiWI BspHI BsrBI BsrGI BstBI BstZ17I CspCI DraI DraIII DrdI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NruI NsiI PacI PciI PmeI PpiI PsiI PspXI PsrI RsrII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SwaI Tth111I XbaI XhoI