(Circular) (Six-base) MAPSORT of: pFN21AB6502.seq Check: 6 from: 1 to: 5848 pFN21AB6502 5848 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3658 278 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3120 3191 1937 Size: 1183 71 4594 Fragments arranged by size: 4594 1183 71 Acc65I G'GTAC_C Cuts at: 3169 3169 Size: 5848 AclI AA'CG_TT Cuts at: 1563 3975 4348 1563 Size: 2412 373 3063 Fragments arranged by size: 3063 2412 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2395 3911 5295 5563 1632 Size: 763 1516 1384 268 1917 Fragments arranged by size: 1917 1516 1384 763 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5651 AflIII A'CryG_T Cuts at: 1203 2682 4747 5724 1203 Size: 1479 2065 977 1327 Fragments arranged by size: 2065 1479 1327 977 AgeI A'CCGG_T Cuts at: 4679 4679 Size: 5848 AhdI GACnn_n'nnGTC Cuts at: 2545 4577 2545 Size: 2032 3816 AleI CACnn'nnGTG Cuts at: 1166 2288 1166 Size: 1122 4726 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2207 1184 Size: 1023 4825 AlwNI CAG_nnn'CTG Cuts at: 2316 2909 3025 3088 3591 5163 2316 Size: 593 116 63 503 1572 3001 Fragments arranged by size: 3001 1572 593 503 116 63 ApaLI G'TGCA_C Cuts at: 3905 5061 3905 Size: 1156 4692 ApoI r'AATT_y Cuts at: 1493 2693 3157 3383 3535 5644 1493 Size: 1200 464 226 152 2109 1697 Fragments arranged by size: 2109 1697 1200 464 226 152 AseI AT'TA_AT Cuts at: 160 1032 4402 160 Size: 872 3370 1606 Fragments arranged by size: 3370 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 AvaI C'yCGr_G Cuts at: 1870 1943 2179 2348 2504 2850 3173 1870 Size: 73 236 169 156 346 323 4545 Fragments arranged by size: 4545 346 323 236 169 156 73 BamHI G'GATC_C Cuts at: 3178 3545 3178 Size: 367 5481 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2196 2276 3169 4624 618 Size: 325 217 627 409 80 893 1455 1842 Fragments arranged by size: 1842 1455 893 627 409 325 217 80 BanII G_rGCy'C Cuts at: 1391 2505 3167 1391 Size: 1114 662 4072 Fragments arranged by size: 4072 1114 662 BbeI G_GCGC'C Cuts at: 2200 2200 Size: 5848 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4877 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2565 2599 4039 4073 1903 Size: 34 628 34 1440 34 3678 Fragments arranged by size: 3678 1440 628 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3740 4950 3740 Size: 1210 4638 BclI T'GATC_A Cuts at: 1548 1548 Size: 5848 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4459 136 Size: 107 122 71 631 605 2787 1525 Fragments arranged by size: 2787 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2631 5843 2631 Size: 3212 2636 BlpI GC'TnA_GC Cuts at: 3265 3265 Size: 5848 Bme1580I G_kGCm'C Cuts at: 1790 2031 3909 5065 1790 Size: 241 1878 1156 2573 Fragments arranged by size: 2573 1878 1156 241 BmgBI CAC'GTC Cuts at: 1318 2683 1318 Size: 1365 4483 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2618 2806 3579 Size: 416 53 83 399 24 1201 188 773 Cuts at: 3579 4537 442 Size: 958 1753 Fragments arranged by size: 1753 1201 958 773 416 399 188 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 2815 4508 1239 Size: 866 710 1693 2579 Fragments arranged by size: 2579 1693 866 710 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2345 2354 3973 4838 5136 2345 Size: 9 1619 865 298 3057 Fragments arranged by size: 3057 1619 865 298 9 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2441 2556 4511 882 Size: 617 942 115 1955 2219 Fragments arranged by size: 2219 1955 942 617 115 BsaAI yAC'GTr Cuts at: 493 1204 3639 493 Size: 711 2435 2702 Fragments arranged by size: 2702 2435 711 BsaBI GATnn'nnATC Cuts at: 5842 5842 Size: 5848 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2197 3655 4037 4672 Size: 53 83 186 936 664 1458 382 635 Cuts at: 4672 275 Size: 1451 Fragments arranged by size: 1458 1451 936 664 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4281 4679 4953 5100 5511 4281 Size: 398 274 147 411 4618 Fragments arranged by size: 4618 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5623 Fragments arranged by size: 5623 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5848 BseYI C'CCAG_C Cuts at: 2089 2497 3043 5051 5547 2089 Size: 408 546 2008 496 2390 Fragments arranged by size: 2390 2008 546 496 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5275 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2126 3298 4059 5087 1540 Size: 275 186 125 1172 761 1028 2301 Fragments arranged by size: 2301 1172 1028 761 275 186 125 BsiHKAI G_wGCw'C Cuts at: 2295 3909 3994 5065 2295 Size: 1614 85 1071 3078 Fragments arranged by size: 3078 1614 1071 85 BsmI GAATG_Cn' Cuts at: 3359 3452 3359 Size: 93 5755 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2295 2505 3167 3909 3994 5065 Size: 399 241 264 210 662 742 85 1071 Cuts at: 5065 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 662 399 264 241 210 85 BspEI T'CCGG_A Cuts at: 5511 5511 Size: 5848 BspHI T'CATG_A Cuts at: 3737 5467 3737 Size: 1730 4118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2223 3203 844 Size: 1379 980 3489 Fragments arranged by size: 3489 1379 980 BsrBI CCG'CTC Cuts at: 1394 1823 2101 2901 3735 1394 Size: 429 278 800 834 3507 Fragments arranged by size: 3507 834 800 429 278 BsrDI GCAATG_nn' Cuts at: 65 1248 2440 2878 4343 4517 65 Size: 1183 1192 438 1465 174 1396 Fragments arranged by size: 1465 1396 1192 1183 438 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2121 2218 4492 4679 1700 Size: 110 311 97 2274 187 2869 Fragments arranged by size: 2869 2274 311 187 110 97 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5848 BssHII G'CGCG_C Cuts at: 1925 2224 2226 1925 Size: 299 2 5547 Fragments arranged by size: 5547 299 2 BssSI C'ACGA_G Cuts at: 2464 3908 4920 2464 Size: 1444 1012 3392 Fragments arranged by size: 3392 1444 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5848 BstBI TT'CG_AA Cuts at: 4652 4652 Size: 5848 BstEII G'GTnAC_C Cuts at: 1184 2210 1184 Size: 1026 4822 BstXI CCAn_nnnn'nTGG Cuts at: 2170 2677 2170 Size: 507 5341 BstYI r'GATC_y Cuts at: 1972 2631 3178 3545 3930 3947 5399 5485 5497 Size: 659 547 367 385 17 1452 86 12 Cuts at: 5497 5843 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 659 547 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3121 3121 Size: 5848 Bsu36I CC'TnA_GG Cuts at: 2823 2823 Size: 5848 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5296 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5109 Fragments arranged by size: 5109 683 56 BtsI GCAGTG_nn' Cuts at: 3364 4158 4178 3364 Size: 794 20 5034 Fragments arranged by size: 5034 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5848 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2845 2880 529 Size: 35 2281 35 3497 Fragments arranged by size: 3497 2281 35 35 DraI TTT'AAA Cuts at: 2764 3152 2764 Size: 388 5460 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4855 809 Size: 507 3539 1802 Fragments arranged by size: 3539 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2123 2160 2172 Size: 54 1425 50 275 18 293 37 12 Cuts at: 2172 3295 4184 5813 8 Size: 1123 889 1629 43 Fragments arranged by size: 1629 1425 1123 889 293 275 54 50 43 37 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2123 3295 1537 Size: 275 311 1172 4090 Fragments arranged by size: 4090 1172 311 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2237 2536 3778 1353 Size: 884 299 1242 3423 Fragments arranged by size: 3423 1242 884 299 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4435 4809 4955 387 Size: 1064 138 2846 374 146 1280 Fragments arranged by size: 2846 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2395 2815 3911 4508 5295 5563 Size: 393 473 290 420 1096 597 787 268 Cuts at: 5563 1239 Size: 1524 Fragments arranged by size: 1524 1096 787 597 473 420 393 290 268 EcoNI CCTnn'n_nnAGG Cuts at: 2204 2204 Size: 5848 EcoO109I rG'GnC_Cy Cuts at: 2509 2509 Size: 5848 EcoRI G'AATT_C Cuts at: 2693 3157 3535 2693 Size: 464 378 5006 Fragments arranged by size: 5006 464 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2728 2728 Size: 5848 FspI TGC'GCA Cuts at: 4354 4354 Size: 5848 HaeII r_GCGC'y Cuts at: 1678 2200 2272 4995 1678 Size: 522 72 2723 2531 Fragments arranged by size: 2723 2531 522 72 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2519 2531 2551 2563 4489 Size: 32 135 32 947 12 20 12 1926 Cuts at: 4489 4521 4563 4595 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1926 947 135 42 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 3192 3438 669 Size: 988 281 1254 246 3079 Fragments arranged by size: 3079 1254 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5848 HpaI GTT'AAC Cuts at: 3438 3438 Size: 5848 KasI G'GCGC_C Cuts at: 2196 2196 Size: 5848 KpnI G_GTAC'C Cuts at: 3173 3173 Size: 5848 MfeI C'AATT_G Cuts at: 3447 3447 Size: 5848 MluI A'CGCG_T Cuts at: 5724 5724 Size: 5848 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2019 2628 2667 3012 3132 4962 5146 1305 Size: 714 609 39 345 120 1830 184 2007 Fragments arranged by size: 2007 1830 714 609 345 184 120 39 MscI TGG'CCA Cuts at: 10 64 1489 1832 2174 10 Size: 54 1425 343 342 3684 Fragments arranged by size: 3684 1425 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 2168 2288 3806 4165 4324 518 Size: 648 1002 120 1518 359 159 2042 Fragments arranged by size: 2042 1518 1002 648 359 159 120 MspA1I CmG'CkG Cuts at: 1606 2083 2599 3022 3088 3264 3941 5089 5334 Size: 477 516 423 66 176 677 1148 245 Cuts at: 5334 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 516 477 423 245 176 66 NaeI GCC'GGC Cuts at: 2123 2123 Size: 5848 NarI GG'CG_CC Cuts at: 2197 2197 Size: 5848 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5296 NdeI CA'TA_TG Cuts at: 387 387 Size: 5848 NgoMIV G'CCGG_C Cuts at: 2121 2121 Size: 5848 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5848 NotI GC'GGCC_GC Cuts at: 3295 3295 Size: 5848 NspI r_CATG'y Cuts at: 1132 1229 3206 4751 1132 Size: 97 1977 1545 2229 Fragments arranged by size: 2229 1977 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5848 PciI A'CATG_T Cuts at: 4747 4747 Size: 5848 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5848 PmeI GTTT'AAAC Cuts at: 3152 3152 Size: 5848 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3917 3949 5430 5462 3917 Size: 32 1481 32 4303 Fragments arranged by size: 4303 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5848 PsiI TTA'TAA Cuts at: 3418 3418 Size: 5848 PstI C_TGCA'G Cuts at: 830 2205 3200 830 Size: 1375 995 3478 Fragments arranged by size: 3478 1375 995 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5848 PvuII CAG'CTG Cuts at: 2083 3022 3088 2083 Size: 939 66 4843 Fragments arranged by size: 4843 939 66 SalI G'TCGA_C Cuts at: 1936 3190 1936 Size: 1254 4594 SapI GCTCTTCn'nnn_ Cuts at: 2237 2237 Size: 5848 SbfI CC_TGCA'GG Cuts at: 3200 3200 Size: 5848 ScaI AGT'ACT Cuts at: 4096 5808 4096 Size: 1712 4136 SexAI A'CCwGG_T Cuts at: 3123 3123 Size: 5848 SfcI C'TryA_G Cuts at: 826 1045 2201 3196 4331 5012 5203 826 Size: 219 1156 995 1135 681 191 1471 Fragments arranged by size: 1471 1156 1135 995 681 219 191 SfoI GGC'GCC Cuts at: 2198 2198 Size: 5848 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5848 SmaI CCC'GGG Cuts at: 1872 2181 2852 3175 1872 Size: 309 671 323 4545 Fragments arranged by size: 4545 671 323 309 SmlI C'TyrA_G Cuts at: 820 1017 1943 2360 2369 3952 4853 5115 820 Size: 197 926 417 9 1583 901 262 1553 Fragments arranged by size: 1583 1553 926 901 417 262 197 9 SnaBI TAC'GTA Cuts at: 493 3639 493 Size: 3146 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5848 SphI G_CATG'C Cuts at: 1132 3206 1132 Size: 2074 3774 SspI AAT'ATT Cuts at: 5 52 3772 5 Size: 47 3720 2081 Fragments arranged by size: 3720 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5848 StyI C'CwwG_G Cuts at: 513 1065 3287 513 Size: 552 2222 3074 Fragments arranged by size: 3074 2222 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2574 3875 3892 4045 4230 4675 325 Size: 1255 994 1301 17 153 185 445 1498 Fragments arranged by size: 1498 1301 1255 994 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2706 4094 5806 Size: 274 80 33 51 1443 729 1388 1712 Cuts at: 5806 96 Size: 138 Fragments arranged by size: 1712 1443 1388 729 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5808 5840 5808 Size: 32 5816 Tth111I GACn'n_nGTC Cuts at: 1316 2969 1316 Size: 1653 4195 XbaI T'CTAG_A Cuts at: 2455 3184 2455 Size: 729 5119 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5848 XmaI C'CCGG_G Cuts at: 1870 2179 2850 3173 1870 Size: 309 671 323 4545 Fragments arranged by size: 4545 671 323 309 XmnI GAAnn'nnTTC Cuts at: 3977 3977 Size: 5848 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3656 276 Size: 53 83 186 936 2122 2468 Fragments arranged by size: 2468 2122 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI