(Circular) (Six-base) MAPSORT of: pFN21AB6552.seq Check: 379 from: 1 to: 5887 pFN21AB6552 5887 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3697 278 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2325 3230 1937 Size: 388 905 4594 Fragments arranged by size: 4594 905 388 Acc65I G'GTAC_C Cuts at: 3208 3208 Size: 5887 AclI AA'CG_TT Cuts at: 1563 4014 4387 1563 Size: 2451 373 3063 Fragments arranged by size: 3063 2451 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2950 3085 3950 5334 5602 1632 Size: 1318 135 865 1384 268 1917 Fragments arranged by size: 1917 1384 1318 865 268 135 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5690 AflIII A'CryG_T Cuts at: 1203 4786 5763 1203 Size: 3583 977 1327 Fragments arranged by size: 3583 1327 977 AgeI A'CCGG_T Cuts at: 4718 4718 Size: 5887 AhdI GACnn_n'nnGTC Cuts at: 4616 4616 Size: 5887 AleI CACnn'nnGTG Cuts at: 1166 2219 1166 Size: 1053 4834 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2156 1184 Size: 972 4915 AlwNI CAG_nnn'CTG Cuts at: 2263 2395 2559 2775 2986 3007 3630 5202 2263 Size: 132 164 216 211 21 623 1572 2948 Fragments arranged by size: 2948 1572 623 216 211 164 132 21 ApaLI G'TGCA_C Cuts at: 2837 3125 3944 5100 2837 Size: 288 819 1156 3624 Fragments arranged by size: 3624 1156 819 288 ApoI r'AATT_y Cuts at: 1493 2471 3196 3422 3574 5683 1493 Size: 978 725 226 152 2109 1697 Fragments arranged by size: 2109 1697 978 725 226 152 AseI AT'TA_AT Cuts at: 160 1032 4441 160 Size: 872 3409 1606 Fragments arranged by size: 3409 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 AvaI C'yCGr_G Cuts at: 1870 1943 3043 3212 1870 Size: 73 1100 169 4545 Fragments arranged by size: 4545 1100 169 73 AvrII C'CTAG_G Cuts at: 2188 2188 Size: 5887 BamHI G'GATC_C Cuts at: 3217 3584 3217 Size: 367 5520 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2108 3208 4663 618 Size: 325 217 627 321 1100 1455 1842 Fragments arranged by size: 1842 1455 1100 627 325 321 217 BanII G_rGCy'C Cuts at: 1391 2243 2538 3206 1391 Size: 852 295 668 4072 Fragments arranged by size: 4072 852 668 295 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3091 928 Size: 971 1192 3724 Fragments arranged by size: 3724 1192 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4078 4112 1903 Size: 34 2141 34 3678 Fragments arranged by size: 3678 2141 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3779 4989 3779 Size: 1210 4677 BclI T'GATC_A Cuts at: 1548 1548 Size: 5887 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2850 4498 136 Size: 107 122 71 631 605 1178 1648 1525 Fragments arranged by size: 1648 1525 1178 631 605 122 107 71 BglII A'GATC_T Cuts at: 5882 5882 Size: 5887 BlpI GC'TnA_GC Cuts at: 3304 3304 Size: 5887 Bme1580I G_kGCm'C Cuts at: 1790 2841 3129 3948 5104 1790 Size: 1051 288 819 1156 2573 Fragments arranged by size: 2573 1156 1051 819 288 BmgBI CAC'GTC Cuts at: 1318 3015 1318 Size: 1697 4190 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2517 2735 3618 Size: 416 53 83 399 24 1100 218 883 Cuts at: 3618 4576 442 Size: 958 1753 Fragments arranged by size: 1753 1100 958 883 416 399 218 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3039 1056 Size: 1983 3904 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2273 2933 4547 1239 Size: 1034 660 1614 2579 Fragments arranged by size: 2579 1614 1034 660 Bpu10I CC'TnA_GC Cuts at: 2235 2235 Size: 5887 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4012 4877 5175 4012 Size: 865 298 4724 Fragments arranged by size: 4724 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3118 4550 882 Size: 617 1619 1432 2219 Fragments arranged by size: 2219 1619 1432 617 BsaAI yAC'GTr Cuts at: 493 1204 3678 493 Size: 711 2474 2702 Fragments arranged by size: 2702 2474 711 BsaBI GATnn'nnATC Cuts at: 5881 5881 Size: 5887 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2658 3694 4076 4711 Size: 53 83 186 936 1125 1036 382 635 Cuts at: 4711 275 Size: 1451 Fragments arranged by size: 1451 1125 1036 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3109 4320 4718 4992 5139 5550 3109 Size: 1211 398 274 147 411 3446 Fragments arranged by size: 3446 1211 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2756 2786 3160 3190 1178 Size: 30 165 30 1353 30 374 30 3875 Fragments arranged by size: 3875 1353 374 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2288 2291 2680 2930 1186 Size: 847 255 3 389 250 4143 Fragments arranged by size: 4143 847 389 255 250 3 BseYI C'CCAG_C Cuts at: 3137 3179 5090 5586 3137 Size: 42 1911 496 3438 Fragments arranged by size: 3438 1911 496 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2408 3140 1163 Size: 573 672 732 3910 Fragments arranged by size: 3910 732 672 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2864 3337 4098 5126 1540 Size: 275 186 863 473 761 1028 2301 Fragments arranged by size: 2301 1028 863 761 473 275 186 BsiHKAI G_wGCw'C Cuts at: 2049 2841 3129 3948 4033 5104 2049 Size: 792 288 819 85 1071 2832 Fragments arranged by size: 2832 1071 819 792 288 85 BsmI GAATG_Cn' Cuts at: 3398 3491 3398 Size: 93 5794 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2243 2538 2841 3129 3206 3948 Size: 399 259 194 295 303 288 77 742 Cuts at: 3948 4033 5104 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 303 295 288 259 194 85 77 BspEI T'CCGG_A Cuts at: 5550 5550 Size: 5887 BspHI T'CATG_A Cuts at: 3776 5506 3776 Size: 1730 4157 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3162 3242 844 Size: 2318 80 3489 Fragments arranged by size: 3489 2318 80 BsrBI CCG'CTC Cuts at: 1394 1823 2127 3061 3774 1394 Size: 429 304 934 713 3507 Fragments arranged by size: 3507 934 713 429 304 BsrDI GCAATG_nn' Cuts at: 65 1248 4382 4556 65 Size: 1183 3134 174 1396 Fragments arranged by size: 3134 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4531 4718 1700 Size: 110 2721 187 2869 Fragments arranged by size: 2869 2721 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5887 BssHII G'CGCG_C Cuts at: 1925 2344 1925 Size: 419 5468 BssSI C'ACGA_G Cuts at: 3947 4959 3947 Size: 1012 4875 BstAPI GCAn_nnn'nTGC Cuts at: 794 2263 2395 2986 794 Size: 1469 132 591 3695 Fragments arranged by size: 3695 1469 591 132 BstBI TT'CG_AA Cuts at: 4691 4691 Size: 5887 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5887 BstXI CCAn_nnnn'nTGG Cuts at: 2494 2878 2494 Size: 384 5503 BstYI r'GATC_y Cuts at: 1972 2259 2598 3217 3584 3969 3986 5438 5524 Size: 287 339 619 367 385 17 1452 86 Cuts at: 5524 5536 5882 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 619 385 367 346 339 287 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2104 2821 3117 513 Size: 552 939 100 717 296 3283 Fragments arranged by size: 3283 939 717 552 296 100 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5148 Fragments arranged by size: 5148 683 56 BtsI GCAGTG_nn' Cuts at: 2047 2629 2839 3403 4197 4217 2047 Size: 582 210 564 794 20 3717 Fragments arranged by size: 3717 794 582 564 210 20 ClaI AT'CG_AT Cuts at: 1554 2802 1554 Size: 1248 4639 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5852 DraI TTT'AAA Cuts at: 3191 3191 Size: 5887 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4894 809 Size: 507 3578 1802 Fragments arranged by size: 3578 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2865 3009 3334 Size: 54 1425 50 275 18 1035 144 325 Cuts at: 3334 4223 5852 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1035 889 325 275 144 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3334 1537 Size: 275 1522 4090 Fragments arranged by size: 4090 1522 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3817 1353 Size: 2464 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4474 4848 4994 387 Size: 1064 138 2885 374 146 1280 Fragments arranged by size: 2885 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2933 2950 3085 3950 4547 5334 Size: 393 641 660 17 135 865 597 787 Cuts at: 5334 5602 1239 Size: 268 1524 Fragments arranged by size: 1524 865 787 660 641 597 393 268 135 17 EcoNI CCTnn'n_nnAGG Cuts at: 2360 2360 Size: 5887 EcoO109I rG'GnC_Cy Cuts at: 2491 2565 2588 3047 2491 Size: 74 23 459 5331 Fragments arranged by size: 5331 459 74 23 EcoRI G'AATT_C Cuts at: 2471 3196 3574 2471 Size: 725 378 4784 Fragments arranged by size: 4784 725 378 FspI TGC'GCA Cuts at: 4393 4393 Size: 5887 HaeII r_GCGC'y Cuts at: 1678 2396 2944 5034 1678 Size: 718 548 2090 2531 Fragments arranged by size: 2531 2090 718 548 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2756 2788 4528 4560 4602 Size: 32 135 32 1184 32 1740 32 42 Cuts at: 4602 4634 1373 Size: 32 2626 Fragments arranged by size: 2626 1740 1184 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2200 3231 3477 669 Size: 988 281 262 1031 246 3079 Fragments arranged by size: 3079 1031 988 281 262 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5887 HpaI GTT'AAC Cuts at: 3477 3477 Size: 5887 KpnI G_GTAC'C Cuts at: 3212 3212 Size: 5887 MfeI C'AATT_G Cuts at: 3486 3486 Size: 5887 MluI A'CGCG_T Cuts at: 5763 5763 Size: 5887 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5001 5185 1305 Size: 3696 184 2007 Fragments arranged by size: 3696 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3011 10 Size: 54 1425 343 1179 2886 Fragments arranged by size: 2886 1425 1179 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2219 2741 3845 4204 4363 518 Size: 648 1053 522 1104 359 159 2042 Fragments arranged by size: 2042 1104 1053 648 522 359 159 MspA1I CmG'CkG Cuts at: 1606 2097 2983 3183 3303 3980 5128 5373 1606 Size: 491 886 200 120 677 1148 245 2120 Fragments arranged by size: 2120 1148 886 677 491 245 200 120 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4396 Fragments arranged by size: 4396 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5887 NheI G'CTAG_C Cuts at: 1052 3035 1052 Size: 1983 3904 NotI GC'GGCC_GC Cuts at: 3334 3334 Size: 5887 NspI r_CATG'y Cuts at: 1132 1229 2653 3245 4790 1132 Size: 97 1424 592 1545 2229 Fragments arranged by size: 2229 1545 1424 592 97 PasI CC'CwG_GG Cuts at: 1348 2113 2662 1348 Size: 765 549 4573 Fragments arranged by size: 4573 765 549 PciI A'CATG_T Cuts at: 4786 4786 Size: 5887 PflMI CCAn_nnn'nTGG Cuts at: 1108 3007 1108 Size: 1899 3988 PmeI GTTT'AAAC Cuts at: 3191 3191 Size: 5887 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3956 3988 5469 5501 3956 Size: 32 1481 32 4342 Fragments arranged by size: 4342 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2565 2588 3047 2565 Size: 23 459 5405 Fragments arranged by size: 5405 459 23 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5887 PsiI TTA'TAA Cuts at: 3457 3457 Size: 5887 PstI C_TGCA'G Cuts at: 830 2042 2156 2555 2991 3239 830 Size: 1212 114 399 436 248 3478 Fragments arranged by size: 3478 1212 436 399 248 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5887 PvuII CAG'CTG Cuts at: 2983 3183 2983 Size: 200 5687 SalI G'TCGA_C Cuts at: 1936 3229 1936 Size: 1293 4594 SanDI GG'GwC_CC Cuts at: 2565 3047 2565 Size: 482 5405 SbfI CC_TGCA'GG Cuts at: 3239 3239 Size: 5887 ScaI AGT'ACT Cuts at: 4135 5847 4135 Size: 1712 4175 SfcI C'TryA_G Cuts at: 826 1045 2038 2152 2551 2987 3235 4370 5051 Size: 219 993 114 399 436 248 1135 681 Cuts at: 5051 5242 826 Size: 191 1471 Fragments arranged by size: 1471 1135 993 681 436 399 248 219 191 114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5887 SmaI CCC'GGG Cuts at: 1872 3214 1872 Size: 1342 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3991 4892 5154 820 Size: 197 926 2048 901 262 1553 Fragments arranged by size: 2048 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3678 493 Size: 3185 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5887 SphI G_CATG'C Cuts at: 1132 3245 1132 Size: 2113 3774 SspI AAT'ATT Cuts at: 5 52 3811 5 Size: 47 3759 2081 Fragments arranged by size: 3759 2081 47 StuI AGG'CCT Cuts at: 1517 2921 1517 Size: 1404 4483 StyI C'CwwG_G Cuts at: 513 1065 2004 2188 2487 2538 3326 513 Size: 552 939 184 299 51 788 3074 Fragments arranged by size: 3074 939 788 552 299 184 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2185 3914 3931 4084 4269 4714 325 Size: 1255 605 1729 17 153 185 445 1498 Fragments arranged by size: 1729 1498 1255 605 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2727 2796 4133 Size: 274 80 33 51 1443 750 69 1337 Cuts at: 4133 5845 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1337 750 274 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3160 3192 5847 5879 3160 Size: 32 2655 32 3168 Fragments arranged by size: 3168 2655 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5887 XbaI T'CTAG_A Cuts at: 3223 3223 Size: 5887 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2536 2875 2011 Size: 525 339 5023 Fragments arranged by size: 5023 525 339 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5887 XmaI C'CCGG_G Cuts at: 1870 3212 1870 Size: 1342 4545 XmnI GAAnn'nnTTC Cuts at: 4016 4016 Size: 5887 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3695 276 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI