(Circular) (Six-base) MAPSORT of: pFN21AB6533.seq Check: 3087 from: 1 to: 5806 pFN21AB6533 5806 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3616 278 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2423 3149 1937 Size: 486 726 4594 Fragments arranged by size: 4594 726 486 Acc65I G'GTAC_C Cuts at: 3127 3127 Size: 5806 AclI AA'CG_TT Cuts at: 1563 3933 4306 1563 Size: 2370 373 3063 Fragments arranged by size: 3063 2370 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2131 2884 3059 3869 5253 5521 1632 Size: 499 753 175 810 1384 268 1917 Fragments arranged by size: 1917 1384 810 753 499 268 175 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5609 AflIII A'CryG_T Cuts at: 1203 4705 5682 1203 Size: 3502 977 1327 Fragments arranged by size: 3502 1327 977 AgeI A'CCGG_T Cuts at: 4637 4637 Size: 5806 AhdI GACnn_n'nnGTC Cuts at: 4535 4535 Size: 5806 AleI CACnn'nnGTG Cuts at: 1166 2156 3014 1166 Size: 990 858 3958 Fragments arranged by size: 3958 990 858 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2174 2600 1184 Size: 990 426 4390 Fragments arranged by size: 4390 990 426 AlwNI CAG_nnn'CTG Cuts at: 2493 2709 2920 2941 2966 3549 5121 2493 Size: 216 211 21 25 583 1572 3178 Fragments arranged by size: 3178 1572 583 216 211 25 21 ApaLI G'TGCA_C Cuts at: 3863 5019 3863 Size: 1156 4650 ApoI r'AATT_y Cuts at: 1493 3115 3341 3493 5602 1493 Size: 1622 226 152 2109 1697 Fragments arranged by size: 2109 1697 1622 226 152 AseI AT'TA_AT Cuts at: 160 1032 4360 160 Size: 872 3328 1606 Fragments arranged by size: 3328 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 AvaI C'yCGr_G Cuts at: 1870 1943 3131 1870 Size: 73 1188 4545 Fragments arranged by size: 4545 1188 73 BamHI G'GATC_C Cuts at: 3136 3503 3136 Size: 367 5439 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 2163 2177 2237 2288 Size: 325 217 627 234 142 14 60 51 Cuts at: 2288 2303 2999 3127 4582 618 Size: 15 696 128 1455 1842 Fragments arranged by size: 1842 1455 696 627 325 234 217 142 128 60 51 15 14 BanII G_rGCy'C Cuts at: 1391 2217 2439 2975 3083 3125 1391 Size: 826 222 536 108 42 4072 Fragments arranged by size: 4072 826 536 222 108 42 BbeI G_GCGC'C Cuts at: 2167 2181 2241 2292 2167 Size: 14 60 51 5681 Fragments arranged by size: 5681 60 51 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2350 928 Size: 971 451 4384 Fragments arranged by size: 4384 971 451 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3997 4031 1903 Size: 34 2060 34 3678 Fragments arranged by size: 3678 2060 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2369 3698 4908 2369 Size: 1329 1210 3267 Fragments arranged by size: 3267 1329 1210 BclI T'GATC_A Cuts at: 1548 2613 1548 Size: 1065 4741 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2318 2784 4417 Size: 107 122 71 631 605 646 466 1633 Cuts at: 4417 136 Size: 1525 Fragments arranged by size: 1633 1525 646 631 605 466 122 107 71 BglII A'GATC_T Cuts at: 5801 5801 Size: 5806 BlpI GC'TnA_GC Cuts at: 2438 3065 3223 2438 Size: 627 158 5021 Fragments arranged by size: 5021 627 158 Bme1580I G_kGCm'C Cuts at: 1790 2024 2205 3867 5023 1790 Size: 234 181 1662 1156 2573 Fragments arranged by size: 2573 1662 1156 234 181 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5806 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3537 4495 442 Size: 416 53 83 399 24 2120 958 1753 Fragments arranged by size: 2120 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5806 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2055 2210 2775 4466 1239 Size: 816 155 565 1691 2579 Fragments arranged by size: 2579 1691 816 565 155 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3931 4796 5094 3931 Size: 865 298 4643 Fragments arranged by size: 4643 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4469 882 Size: 617 2970 2219 Fragments arranged by size: 2970 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3597 493 Size: 711 2393 2702 Fragments arranged by size: 2702 2393 711 BsaBI GATnn'nnATC Cuts at: 5800 5800 Size: 5806 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2164 2178 2238 2289 Size: 53 83 186 936 631 14 60 51 Cuts at: 2289 3613 3995 4630 275 Size: 1324 382 635 1451 Fragments arranged by size: 1451 1324 936 635 631 382 186 83 60 53 51 14 BsaWI w'CCGG_w Cuts at: 4239 4637 4911 5058 5469 4239 Size: 398 274 147 411 4576 Fragments arranged by size: 4576 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2078 2108 2690 2720 2748 Size: 30 165 30 675 30 582 30 28 Cuts at: 2748 2778 3079 3109 1178 Size: 30 301 30 3875 Fragments arranged by size: 3875 675 582 301 165 30 30 30 30 30 30 28 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5806 BseYI C'CCAG_C Cuts at: 2053 2966 3098 5009 5505 2053 Size: 913 132 1911 496 2354 Fragments arranged by size: 2354 1911 913 496 132 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2431 2506 1163 Size: 573 695 75 4463 Fragments arranged by size: 4463 695 573 75 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2224 2322 2995 3256 4017 5045 Size: 275 186 223 98 673 261 761 1028 Cuts at: 5045 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 673 275 261 223 186 98 BsiHKAI G_wGCw'C Cuts at: 2196 2217 2991 3867 3952 5023 2196 Size: 21 774 876 85 1071 2979 Fragments arranged by size: 2979 1071 876 774 85 21 BsmI GAATG_Cn' Cuts at: 2755 3317 3410 2755 Size: 562 93 5151 Fragments arranged by size: 5151 562 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2626 3037 2626 Size: 411 5395 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2196 2205 2217 2439 2975 2991 Size: 399 234 172 9 12 222 536 16 Cuts at: 2991 3083 3125 3867 3952 5023 1391 Size: 92 42 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 536 399 234 222 172 92 85 42 16 12 9 BspEI T'CCGG_A Cuts at: 5469 5469 Size: 5806 BspHI T'CATG_A Cuts at: 3695 5425 3695 Size: 1730 4076 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3161 844 Size: 2317 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2066 2094 3693 1394 Size: 429 243 28 1599 3507 Fragments arranged by size: 3507 1599 429 243 28 BsrDI GCAATG_nn' Cuts at: 65 1248 2078 4301 4475 65 Size: 1183 830 2223 174 1396 Fragments arranged by size: 2223 1396 1183 830 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2114 2126 2234 2321 4450 4637 1700 Size: 110 304 12 108 87 2129 187 2869 Fragments arranged by size: 2869 2129 304 187 110 108 87 12 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5806 BssHII G'CGCG_C Cuts at: 1925 2325 2342 2344 1925 Size: 400 17 2 5387 Fragments arranged by size: 5387 400 17 2 BssSI C'ACGA_G Cuts at: 3866 4878 3866 Size: 1012 4794 BstAPI GCAn_nnn'nTGC Cuts at: 794 2077 2200 2493 794 Size: 1283 123 293 4107 Fragments arranged by size: 4107 1283 293 123 BstBI TT'CG_AA Cuts at: 4610 4610 Size: 5806 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5806 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2188 2011 Size: 177 5629 BstYI r'GATC_y Cuts at: 1972 3136 3503 3888 3905 5357 5443 5455 5801 Size: 1164 367 385 17 1452 86 12 346 Cuts at: 5801 1972 Size: 1977 Fragments arranged by size: 1977 1452 1164 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2181 2218 2250 2430 2979 513 Size: 552 939 177 37 32 180 549 3340 Fragments arranged by size: 3340 939 552 549 180 177 37 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2107 487 Size: 683 56 881 4186 Fragments arranged by size: 4186 881 683 56 BtsI GCAGTG_nn' Cuts at: 2563 2773 3322 4116 4136 2563 Size: 210 549 794 20 4233 Fragments arranged by size: 4233 794 549 210 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5806 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5771 DraI TTT'AAA Cuts at: 3110 3110 Size: 5806 DraIII CAC_nnn'GTG Cuts at: 2200 2251 2200 Size: 51 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4813 809 Size: 507 3497 1802 Fragments arranged by size: 3497 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2116 2221 2232 Size: 54 1425 50 275 18 286 105 11 Cuts at: 2232 2319 3253 4142 5771 8 Size: 87 934 889 1629 43 Fragments arranged by size: 1629 1425 934 889 286 275 105 87 54 50 43 18 11 EagI C'GGCC_G Cuts at: 1537 1812 2221 2319 3253 1537 Size: 275 409 98 934 4090 Fragments arranged by size: 4090 934 409 275 98 EarI CTCTTCn'nnn_ Cuts at: 1353 3736 1353 Size: 2383 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4393 4767 4913 387 Size: 1064 138 2804 374 146 1280 Fragments arranged by size: 2804 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2215 2215 Size: 5806 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2131 2210 2775 2884 3059 3869 Size: 393 423 76 79 565 109 175 810 Cuts at: 3869 4466 5253 5521 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 810 787 597 565 423 393 268 175 109 79 76 EcoRI G'AATT_C Cuts at: 3115 3493 3115 Size: 378 5428 FspI TGC'GCA Cuts at: 4312 4312 Size: 5806 HaeII r_GCGC'y Cuts at: 1678 2051 2167 2181 2241 2277 2292 4953 1678 Size: 373 116 14 60 36 15 2661 2531 Fragments arranged by size: 2661 2531 373 116 60 36 15 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2078 2110 2690 2722 2818 Size: 32 135 32 506 32 580 32 96 Cuts at: 2818 2850 4447 4479 4521 4553 1373 Size: 32 1597 32 42 32 2626 Fragments arranged by size: 2626 1597 580 506 135 96 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2137 2424 3150 3396 669 Size: 988 281 199 287 726 246 3079 Fragments arranged by size: 3079 988 726 287 281 246 199 HindIII A'AGCT_T Cuts at: 748 748 Size: 5806 HpaI GTT'AAC Cuts at: 3396 3396 Size: 5806 KasI G'GCGC_C Cuts at: 2163 2177 2237 2288 2163 Size: 14 60 51 5681 Fragments arranged by size: 5681 60 51 14 KpnI G_GTAC'C Cuts at: 3131 3131 Size: 5806 MfeI C'AATT_G Cuts at: 2629 3405 2629 Size: 776 5030 MluI A'CGCG_T Cuts at: 5682 5682 Size: 5806 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4920 5104 1305 Size: 3615 184 2007 Fragments arranged by size: 3615 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3984 Fragments arranged by size: 3984 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2015 2156 2675 2984 3014 3764 Size: 648 843 6 141 519 309 30 750 Cuts at: 3764 4123 4282 518 Size: 359 159 2042 Fragments arranged by size: 2042 843 750 648 519 359 309 159 141 30 6 MspA1I CmG'CkG Cuts at: 1606 2220 2917 3102 3222 3899 5047 5292 1606 Size: 614 697 185 120 677 1148 245 2120 Fragments arranged by size: 2120 1148 697 677 614 245 185 120 NaeI GCC'GGC Cuts at: 2116 2128 2236 2323 2116 Size: 12 108 87 5599 Fragments arranged by size: 5599 108 87 12 NarI GG'CG_CC Cuts at: 2164 2178 2238 2289 2164 Size: 14 60 51 5681 Fragments arranged by size: 5681 60 51 14 NcoI C'CATG_G Cuts at: 513 1065 2004 2181 2430 2979 513 Size: 552 939 177 249 549 3340 Fragments arranged by size: 3340 939 552 549 249 177 NdeI CA'TA_TG Cuts at: 387 387 Size: 5806 NgoMIV G'CCGG_C Cuts at: 2114 2126 2234 2321 2114 Size: 12 108 87 5599 Fragments arranged by size: 5599 108 87 12 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5806 NotI GC'GGCC_GC Cuts at: 2221 3253 2221 Size: 1032 4774 NspI r_CATG'y Cuts at: 1132 1229 2587 3164 4709 1132 Size: 97 1358 577 1545 2229 Fragments arranged by size: 2229 1545 1358 577 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5806 PciI A'CATG_T Cuts at: 4705 4705 Size: 5806 PflMI CCAn_nnn'nTGG Cuts at: 1108 2251 2941 1108 Size: 1143 690 3973 Fragments arranged by size: 3973 1143 690 PmeI GTTT'AAAC Cuts at: 3110 3110 Size: 5806 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3875 3907 5388 5420 3875 Size: 32 1481 32 4261 Fragments arranged by size: 4261 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5806 PsiI TTA'TAA Cuts at: 3376 3376 Size: 5806 PstI C_TGCA'G Cuts at: 830 2498 2925 3158 830 Size: 1668 427 233 3478 Fragments arranged by size: 3478 1668 427 233 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5806 PvuII CAG'CTG Cuts at: 2917 3102 2917 Size: 185 5621 SacI G_AGCT'C Cuts at: 2217 2217 Size: 5806 SacII CC_GC'GG Cuts at: 2221 2221 Size: 5806 SalI G'TCGA_C Cuts at: 1936 2422 3148 1936 Size: 486 726 4594 Fragments arranged by size: 4594 726 486 SbfI CC_TGCA'GG Cuts at: 3158 3158 Size: 5806 ScaI AGT'ACT Cuts at: 2455 4054 5766 2455 Size: 1599 1712 2495 Fragments arranged by size: 2495 1712 1599 SfcI C'TryA_G Cuts at: 826 1045 2494 2921 3154 4289 4970 5161 826 Size: 219 1449 427 233 1135 681 191 1471 Fragments arranged by size: 1471 1449 1135 681 427 233 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2318 2318 Size: 5806 SfoI GGC'GCC Cuts at: 2165 2179 2239 2290 2165 Size: 14 60 51 5681 Fragments arranged by size: 5681 60 51 14 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5806 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5806 SmaI CCC'GGG Cuts at: 1872 3133 1872 Size: 1261 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3910 4811 5073 820 Size: 197 926 1967 901 262 1553 Fragments arranged by size: 1967 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3597 493 Size: 3104 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5806 SphI G_CATG'C Cuts at: 1132 3164 1132 Size: 2032 3774 SspI AAT'ATT Cuts at: 5 52 3730 5 Size: 47 3678 2081 Fragments arranged by size: 3678 2081 47 StuI AGG'CCT Cuts at: 1517 2040 2855 1517 Size: 523 815 4468 Fragments arranged by size: 4468 815 523 StyI C'CwwG_G Cuts at: 513 1065 2004 2172 2181 2430 2595 2979 3245 Size: 552 939 168 9 249 165 384 266 Cuts at: 3245 513 Size: 3074 Fragments arranged by size: 3074 939 552 384 266 249 168 165 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2472 3009 3833 3850 4003 4188 4633 Size: 1255 892 537 824 17 153 185 445 Cuts at: 4633 325 Size: 1498 Fragments arranged by size: 1498 1255 892 824 537 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2453 2661 4052 Size: 274 80 33 51 1443 476 208 1391 Cuts at: 4052 5764 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1391 476 274 208 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2468 2500 2748 2780 5766 5798 2468 Size: 32 248 32 2986 32 2476 Fragments arranged by size: 2986 2476 248 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5806 XbaI T'CTAG_A Cuts at: 3142 3142 Size: 5806 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2179 2179 Size: 5806 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5806 XmaI C'CCGG_G Cuts at: 1870 3131 1870 Size: 1261 4545 XmnI GAAnn'nnTTC Cuts at: 3935 3935 Size: 5806 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3614 276 Size: 53 83 186 936 2080 2468 Fragments arranged by size: 2468 2080 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SrfI SwaI