(Circular) (Six-base) MAPSORT of: pFN21AB6446.seq Check: 7879 from: 1 to: 5746 pFN21AB6446 5746 bp With 182 enzymes: SgfI * October 20, 2009 10:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2054 2501 2575 2054 Size: 447 74 5225 Fragments arranged by size: 5225 447 74 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3556 278 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3089 1937 Size: 1152 4594 Acc65I G'GTAC_C Cuts at: 2979 3067 2979 Size: 88 5658 AclI AA'CG_TT Cuts at: 1563 3873 4246 1563 Size: 2310 373 3063 Fragments arranged by size: 3063 2310 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2303 3809 5193 5461 1632 Size: 671 1506 1384 268 1917 Fragments arranged by size: 1917 1506 1384 671 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5549 AflIII A'CryG_T Cuts at: 1203 4645 5622 1203 Size: 3442 977 1327 Fragments arranged by size: 3442 1327 977 AgeI A'CCGG_T Cuts at: 4577 4577 Size: 5746 AhdI GACnn_n'nnGTC Cuts at: 2915 4475 2915 Size: 1560 4186 AleI CACnn'nnGTG Cuts at: 1166 2798 1166 Size: 1632 4114 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2852 1184 Size: 1668 4078 AlwNI CAG_nnn'CTG Cuts at: 3489 5061 3489 Size: 1572 4174 ApaLI G'TGCA_C Cuts at: 3803 4959 3803 Size: 1156 4590 ApoI r'AATT_y Cuts at: 1493 3055 3281 3433 5542 1493 Size: 1562 226 152 2109 1697 Fragments arranged by size: 2109 1697 1562 226 152 AseI AT'TA_AT Cuts at: 160 1032 4300 160 Size: 872 3268 1606 Fragments arranged by size: 3268 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 AvaI C'yCGr_G Cuts at: 1870 1943 2409 2574 3003 3071 1870 Size: 73 466 165 429 68 4545 Fragments arranged by size: 4545 466 429 165 73 68 BamHI G'GATC_C Cuts at: 3076 3443 3076 Size: 367 5379 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 2711 2789 2979 3067 Size: 325 217 627 255 669 78 190 88 Cuts at: 3067 4522 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 669 627 325 255 217 190 88 78 BanII G_rGCy'C Cuts at: 1391 2229 2410 2418 2581 3065 1391 Size: 838 181 8 163 484 4072 Fragments arranged by size: 4072 838 484 181 163 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2725 928 Size: 971 826 3949 Fragments arranged by size: 3949 971 826 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3937 3971 1903 Size: 34 2000 34 3678 Fragments arranged by size: 3678 2000 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2793 3638 4848 2793 Size: 845 1210 3691 Fragments arranged by size: 3691 1210 845 BclI T'GATC_A Cuts at: 1548 1548 Size: 5746 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2114 2243 2826 Size: 107 122 71 631 605 442 129 583 Cuts at: 2826 4357 136 Size: 1531 1525 Fragments arranged by size: 1531 1525 631 605 583 442 129 122 107 71 BglII A'GATC_T Cuts at: 5741 5741 Size: 5746 BlpI GC'TnA_GC Cuts at: 3163 3163 Size: 5746 Bme1580I G_kGCm'C Cuts at: 1790 3807 4963 1790 Size: 2017 1156 2573 Fragments arranged by size: 2573 2017 1156 BmgBI CAC'GTC Cuts at: 1318 2892 1318 Size: 1574 4172 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3477 4435 442 Size: 416 53 83 399 24 2060 958 1753 Fragments arranged by size: 2060 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 4406 1239 Size: 929 2238 2579 Fragments arranged by size: 2579 2238 929 Bpu10I CC'TnA_GC Cuts at: 2090 2090 Size: 5746 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2234 3871 4736 5034 2234 Size: 1637 865 298 2946 Fragments arranged by size: 2946 1637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2093 2671 2902 4409 882 Size: 617 594 578 231 1507 2219 Fragments arranged by size: 2219 1507 617 594 578 231 BsaAI yAC'GTr Cuts at: 493 1204 3537 493 Size: 711 2333 2702 Fragments arranged by size: 2702 2333 711 BsaBI GATnn'nnATC Cuts at: 5740 5740 Size: 5746 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2676 3553 3935 4570 Size: 53 83 186 936 1143 877 382 635 Cuts at: 4570 275 Size: 1451 Fragments arranged by size: 1451 1143 936 877 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4179 4577 4851 4998 5409 4179 Size: 398 274 147 411 4516 Fragments arranged by size: 4516 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2361 2391 1178 Size: 30 165 30 958 30 4533 Fragments arranged by size: 4533 958 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 2415 2418 2445 2483 3029 1186 Size: 958 271 3 27 38 546 3903 Fragments arranged by size: 3903 958 546 271 38 27 3 BseYI C'CCAG_C Cuts at: 2104 2693 2967 4949 5445 2104 Size: 589 274 1982 496 2405 Fragments arranged by size: 2405 1982 589 496 274 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2938 1163 Size: 573 1202 3971 Fragments arranged by size: 3971 1202 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2614 3196 3957 4985 1540 Size: 275 186 613 582 761 1028 2301 Fragments arranged by size: 2301 1028 761 613 582 275 186 BsiHKAI G_wGCw'C Cuts at: 2136 2346 2410 2492 3807 3892 4963 2136 Size: 210 64 82 1315 85 1071 2919 Fragments arranged by size: 2919 1315 1071 210 85 82 64 BsmI GAATG_Cn' Cuts at: 2527 2548 3257 3350 2527 Size: 21 709 93 4923 Fragments arranged by size: 4923 709 93 21 BsmBI CGTCTCn'nnnn_ Cuts at: 2876 2876 Size: 5746 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2229 2346 2410 2418 2492 2581 Size: 399 346 93 117 64 8 74 89 Cuts at: 2581 3065 3807 3892 4963 1391 Size: 484 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 484 399 346 117 93 89 85 74 64 8 BspEI T'CCGG_A Cuts at: 5409 5409 Size: 5746 BspHI T'CATG_A Cuts at: 3635 5365 3635 Size: 1730 4016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2054 2501 2575 3101 844 Size: 1210 447 74 526 3489 Fragments arranged by size: 3489 1210 526 447 74 BsrBI CCG'CTC Cuts at: 1394 1823 3633 1394 Size: 429 1810 3507 Fragments arranged by size: 3507 1810 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4241 4415 65 Size: 1183 2993 174 1396 Fragments arranged by size: 2993 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2544 4390 4577 1700 Size: 110 734 1846 187 2869 Fragments arranged by size: 2869 1846 734 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5746 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5746 BssSI C'ACGA_G Cuts at: 3806 4818 3806 Size: 1012 4734 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5746 BstBI TT'CG_AA Cuts at: 4550 4550 Size: 5746 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5746 BstXI CCAn_nnnn'nTGG Cuts at: 2506 2533 2800 2975 2506 Size: 27 267 175 5277 Fragments arranged by size: 5277 267 175 27 BstYI r'GATC_y Cuts at: 1972 2514 3076 3443 3828 3845 5297 5383 5395 Size: 542 562 367 385 17 1452 86 12 Cuts at: 5395 5741 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 562 542 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2823 2823 Size: 5746 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5194 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5007 Fragments arranged by size: 5007 683 56 BtsI GCAGTG_nn' Cuts at: 3262 4056 4076 3262 Size: 794 20 4932 Fragments arranged by size: 4932 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5711 DraI TTT'AAA Cuts at: 3050 3050 Size: 5746 DraIII CAC_nnn'GTG Cuts at: 2140 2140 Size: 5746 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4753 809 Size: 507 3437 1802 Fragments arranged by size: 3437 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2611 3193 4082 Size: 54 1425 50 275 18 781 582 889 Cuts at: 4082 5711 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 781 582 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2611 3193 1537 Size: 275 799 582 4090 Fragments arranged by size: 4090 799 582 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2454 2472 3676 1353 Size: 1101 18 1204 3423 Fragments arranged by size: 3423 1204 1101 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2671 4333 4707 4853 387 Size: 1064 138 1082 1662 374 146 1280 Fragments arranged by size: 1662 1280 1082 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2408 2408 Size: 5746 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2303 3809 4406 5193 5461 1239 Size: 393 536 135 1506 597 787 268 1524 Fragments arranged by size: 1524 1506 787 597 536 393 268 135 EcoNI CCTnn'n_nnAGG Cuts at: 2465 2465 Size: 5746 EcoO109I rG'GnC_Cy Cuts at: 2201 2963 2201 Size: 762 4984 EcoRI G'AATT_C Cuts at: 3055 3433 3055 Size: 378 5368 FspI TGC'GCA Cuts at: 4252 4252 Size: 5746 HaeII r_GCGC'y Cuts at: 1678 4893 1678 Size: 3215 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2901 2933 4387 4419 4461 Size: 32 135 32 1329 32 1454 32 42 Cuts at: 4461 4493 1373 Size: 32 2626 Fragments arranged by size: 2626 1454 1329 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3090 3336 669 Size: 988 281 1152 246 3079 Fragments arranged by size: 3079 1152 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5746 HpaI GTT'AAC Cuts at: 3336 3336 Size: 5746 KpnI G_GTAC'C Cuts at: 2983 3071 2983 Size: 88 5658 MfeI C'AATT_G Cuts at: 3345 3345 Size: 5746 MluI A'CGCG_T Cuts at: 5622 5622 Size: 5746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4860 5044 1305 Size: 3555 184 2007 Fragments arranged by size: 3555 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3924 Fragments arranged by size: 3924 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2798 2894 3704 4063 4222 518 Size: 648 1632 96 810 359 159 2042 Fragments arranged by size: 2042 1632 810 648 359 159 96 MspA1I CmG'CkG Cuts at: 1606 2629 2952 2971 3162 3839 4987 5232 1606 Size: 1023 323 19 191 677 1148 245 2120 Fragments arranged by size: 2120 1148 1023 677 323 245 191 19 NaeI GCC'GGC Cuts at: 2546 2546 Size: 5746 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5194 NdeI CA'TA_TG Cuts at: 387 387 Size: 5746 NgoMIV G'CCGG_C Cuts at: 2544 2544 Size: 5746 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5746 NotI GC'GGCC_GC Cuts at: 2611 3193 2611 Size: 582 5164 NspI r_CATG'y Cuts at: 1132 1229 3104 4649 1132 Size: 97 1875 1545 2229 Fragments arranged by size: 2229 1875 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5746 PciI A'CATG_T Cuts at: 4645 4645 Size: 5746 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5746 PfoI T'CCnGG_A Cuts at: 2811 2811 Size: 5746 PmeI GTTT'AAAC Cuts at: 3050 3050 Size: 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3815 3847 5328 5360 3815 Size: 32 1481 32 4201 Fragments arranged by size: 4201 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2201 2963 2201 Size: 762 4984 PshAI GACnn'nnGTC Cuts at: 1613 2868 1613 Size: 1255 4491 PsiI TTA'TAA Cuts at: 3316 3316 Size: 5746 PstI C_TGCA'G Cuts at: 830 2586 3098 830 Size: 1756 512 3478 Fragments arranged by size: 3478 1756 512 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5746 PvuII CAG'CTG Cuts at: 2629 2952 2971 2629 Size: 323 19 5404 Fragments arranged by size: 5404 323 19 SacI G_AGCT'C Cuts at: 2410 2410 Size: 5746 SalI G'TCGA_C Cuts at: 1936 3088 1936 Size: 1152 4594 SanDI GG'GwC_CC Cuts at: 2963 2963 Size: 5746 SapI GCTCTTCn'nnn_ Cuts at: 2472 2472 Size: 5746 SbfI CC_TGCA'GG Cuts at: 3098 3098 Size: 5746 ScaI AGT'ACT Cuts at: 3994 5706 3994 Size: 1712 4034 SfcI C'TryA_G Cuts at: 826 1045 2582 3094 4229 4910 5101 826 Size: 219 1537 512 1135 681 191 1471 Fragments arranged by size: 1537 1471 1135 681 512 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5746 SmaI CCC'GGG Cuts at: 1872 2576 3005 3073 1872 Size: 704 429 68 4545 Fragments arranged by size: 4545 704 429 68 SmlI C'TyrA_G Cuts at: 820 1017 1943 2249 3850 4751 5013 820 Size: 197 926 306 1601 901 262 1553 Fragments arranged by size: 1601 1553 926 901 306 262 197 SnaBI TAC'GTA Cuts at: 493 3537 493 Size: 3044 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5746 SphI G_CATG'C Cuts at: 1132 3104 1132 Size: 1972 3774 SspI AAT'ATT Cuts at: 5 52 3670 5 Size: 47 3618 2081 Fragments arranged by size: 3618 2081 47 StuI AGG'CCT Cuts at: 1517 2597 1517 Size: 1080 4666 StyI C'CwwG_G Cuts at: 513 1065 3185 513 Size: 552 2120 3074 Fragments arranged by size: 3074 2120 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2778 2926 3773 3790 3943 4128 4573 Size: 1255 1198 148 847 17 153 185 445 Cuts at: 4573 325 Size: 1498 Fragments arranged by size: 1498 1255 1198 847 445 185 153 148 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3992 5704 96 Size: 274 80 33 51 1443 2015 1712 138 Fragments arranged by size: 2015 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2126 2158 2361 2393 5706 5738 2126 Size: 32 203 32 3313 32 2134 Fragments arranged by size: 3313 2134 203 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2065 1316 Size: 749 4997 XbaI T'CTAG_A Cuts at: 3082 3082 Size: 5746 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2648 2648 Size: 5746 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5746 XmaI C'CCGG_G Cuts at: 1870 2574 3003 3071 1870 Size: 704 429 68 4545 Fragments arranged by size: 4545 704 429 68 XmnI GAAnn'nnTTC Cuts at: 3875 3875 Size: 5746 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3554 276 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI