(Circular) (Six-base) MAPSORT of: pFN21AB7342.seq Check: 9532 from: 1 to: 7387 pFN21AB7342 7387 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5197 278 Size: 53 83 186 936 3661 2468 Fragments arranged by size: 3661 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3177 4730 1937 Size: 1240 1553 4594 Fragments arranged by size: 4594 1553 1240 Acc65I G'GTAC_C Cuts at: 4708 4708 Size: 7387 AclI AA'CG_TT Cuts at: 1563 5514 5887 1563 Size: 3951 373 3063 Fragments arranged by size: 3951 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2111 2194 2766 4239 5450 6834 7102 1632 Size: 479 83 572 1473 1211 1384 268 1917 Fragments arranged by size: 1917 1473 1384 1211 572 479 268 83 AflII C'TTAA_G Cuts at: 820 1017 3520 820 Size: 197 2503 4687 Fragments arranged by size: 4687 2503 197 AflIII A'CryG_T Cuts at: 1203 2464 4133 6286 7263 1203 Size: 1261 1669 2153 977 1327 Fragments arranged by size: 2153 1669 1327 1261 977 AgeI A'CCGG_T Cuts at: 2903 6218 2903 Size: 3315 4072 AhdI GACnn_n'nnGTC Cuts at: 3753 6116 3753 Size: 2363 5024 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7387 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4690 1184 Size: 3506 3881 AlwNI CAG_nnn'CTG Cuts at: 5130 6702 5130 Size: 1572 5815 ApaI G_GGCC'C Cuts at: 3410 3410 Size: 7387 ApaLI G'TGCA_C Cuts at: 3364 5444 6600 3364 Size: 2080 1156 4151 Fragments arranged by size: 4151 2080 1156 ApoI r'AATT_y Cuts at: 1493 2214 3617 4198 4652 4696 4922 5074 7183 Size: 721 1403 581 454 44 226 152 2109 Cuts at: 7183 1493 Size: 1697 Fragments arranged by size: 2109 1697 1403 721 581 454 226 152 44 AseI AT'TA_AT Cuts at: 160 1032 3564 5941 160 Size: 872 2532 2377 1606 Fragments arranged by size: 2532 2377 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7387 AvaI C'yCGr_G Cuts at: 1870 1943 4712 1870 Size: 73 2769 4545 Fragments arranged by size: 4545 2769 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2892 2925 2892 Size: 33 7354 BamHI G'GATC_C Cuts at: 4717 5084 4717 Size: 367 7020 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4708 6163 618 Size: 325 217 627 2921 1455 1842 Fragments arranged by size: 2921 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2416 3347 3410 4706 1391 Size: 1025 931 63 1296 4072 Fragments arranged by size: 4072 1296 1025 931 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2253 4188 4542 928 Size: 971 354 1935 354 3773 Fragments arranged by size: 3773 1935 971 354 354 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2609 2643 5578 5612 1903 Size: 34 672 34 2935 34 3678 Fragments arranged by size: 3678 2935 672 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3473 5279 6489 3473 Size: 1806 1210 4371 Fragments arranged by size: 4371 1806 1210 BclI T'GATC_A Cuts at: 1548 2688 1548 Size: 1140 6247 BfrBI ATG'CAT Cuts at: 4677 4677 Size: 7387 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5998 136 Size: 107 122 71 631 605 4326 1525 Fragments arranged by size: 4326 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7382 7382 Size: 7387 BlpI GC'TnA_GC Cuts at: 4804 4804 Size: 7387 Bme1580I G_kGCm'C Cuts at: 1790 3368 3410 5448 6604 1790 Size: 1578 42 2038 1156 2573 Fragments arranged by size: 2573 2038 1578 1156 42 BmgBI CAC'GTC Cuts at: 1318 3112 1318 Size: 1794 5593 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2586 5118 6076 Size: 416 53 83 399 24 1169 2532 958 Cuts at: 6076 442 Size: 1753 Fragments arranged by size: 2532 1753 1169 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7387 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2180 3408 4248 4646 6047 1239 Size: 941 1228 840 398 1401 2579 Fragments arranged by size: 2579 1401 1228 941 840 398 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2156 2375 3056 3604 5512 6377 6675 2156 Size: 219 681 548 1908 865 298 2868 Fragments arranged by size: 2868 1908 865 681 548 298 219 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6050 882 Size: 617 4551 2219 Fragments arranged by size: 4551 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5178 493 Size: 711 3974 2702 Fragments arranged by size: 3974 2702 711 BsaBI GATnn'nnATC Cuts at: 7381 7381 Size: 7387 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5194 5576 6211 275 Size: 53 83 186 936 3661 382 635 1451 Fragments arranged by size: 3661 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2190 2774 2903 5820 6218 6492 6639 7050 2190 Size: 584 129 2917 398 274 147 411 2527 Fragments arranged by size: 2917 2527 584 411 398 274 147 129 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2429 2459 4221 4251 1178 Size: 30 165 30 1026 30 1762 30 4314 Fragments arranged by size: 4314 1762 1026 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 3150 4532 1186 Size: 991 973 1382 4041 Fragments arranged by size: 4041 1382 991 973 BseYI C'CCAG_C Cuts at: 3457 4191 6590 7086 3457 Size: 734 2399 496 3758 Fragments arranged by size: 3758 2399 734 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2475 1163 Size: 573 739 6075 Fragments arranged by size: 6075 739 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4606 4837 5598 6626 1540 Size: 275 186 2605 231 761 1028 2301 Fragments arranged by size: 2605 2301 1028 761 275 231 186 BsiHKAI G_wGCw'C Cuts at: 3347 3368 5448 5533 6604 3347 Size: 21 2080 85 1071 4130 Fragments arranged by size: 4130 2080 1071 85 21 BsmI GAATG_Cn' Cuts at: 3419 4898 4991 3419 Size: 1479 93 5815 Fragments arranged by size: 5815 1479 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2299 2398 4041 2299 Size: 99 1643 5645 Fragments arranged by size: 5645 1643 99 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2416 3347 3368 3410 4706 5448 5533 Size: 399 626 931 21 42 1296 742 85 Cuts at: 5533 6604 1391 Size: 1071 2174 Fragments arranged by size: 2174 1296 1071 931 742 626 399 85 42 21 BspEI T'CCGG_A Cuts at: 2774 7050 2774 Size: 4276 3111 BspHI T'CATG_A Cuts at: 5276 7006 5276 Size: 1730 5657 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4742 844 Size: 3898 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5274 1394 Size: 429 3451 3507 Fragments arranged by size: 3507 3451 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5882 6056 65 Size: 1183 4634 174 1396 Fragments arranged by size: 4634 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2903 4580 6031 6218 1700 Size: 110 1093 1677 1451 187 2869 Fragments arranged by size: 2869 1677 1451 1093 187 110 BsrGI T'GTAC_A Cuts at: 96 2591 96 Size: 2495 4892 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7387 BssSI C'ACGA_G Cuts at: 5447 6459 5447 Size: 1012 6375 BstAPI GCAn_nnn'nTGC Cuts at: 794 2651 3662 794 Size: 1857 1011 4519 Fragments arranged by size: 4519 1857 1011 BstBI TT'CG_AA Cuts at: 6191 6191 Size: 7387 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7387 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 7387 BstYI r'GATC_y Cuts at: 1972 2475 3226 3793 4717 5084 5469 5486 6938 Size: 503 751 567 924 367 385 17 1452 Cuts at: 6938 7024 7036 7382 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 924 751 567 503 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3178 3178 Size: 7387 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5896 Fragments arranged by size: 5896 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2100 487 Size: 683 56 874 5774 Fragments arranged by size: 5774 874 683 56 BtsI GCAGTG_nn' Cuts at: 3663 4146 4903 5697 5717 3663 Size: 483 757 794 20 5333 Fragments arranged by size: 5333 794 757 483 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7387 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2667 2702 2791 2826 4369 4404 529 Size: 35 2103 35 89 35 1543 35 3512 Fragments arranged by size: 3512 2103 1543 89 35 35 35 35 DraI TTT'AAA Cuts at: 4691 4691 Size: 7387 DraIII CAC_nnn'GTG Cuts at: 2740 2740 Size: 7387 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4405 6394 809 Size: 507 3089 1989 1802 Fragments arranged by size: 3089 1989 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2178 3463 3670 Size: 54 1425 50 275 18 348 1285 207 Cuts at: 3670 4315 4578 4834 5723 7352 8 Size: 645 263 256 889 1629 43 Fragments arranged by size: 1629 1425 1285 889 645 348 275 263 256 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4834 1537 Size: 275 3022 4090 Fragments arranged by size: 4090 3022 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2360 4611 5317 1353 Size: 1007 2251 706 3423 Fragments arranged by size: 3423 2251 1007 706 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5974 6348 6494 387 Size: 1064 138 4385 374 146 1280 Fragments arranged by size: 4385 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3345 3345 Size: 7387 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2180 2194 2766 3408 4239 4248 Size: 393 479 69 14 572 642 831 9 Cuts at: 4248 4646 5450 6047 6834 7102 1239 Size: 398 804 597 787 268 1524 Fragments arranged by size: 1524 831 804 787 642 597 572 479 398 393 268 69 14 9 EcoO109I rG'GnC_Cy Cuts at: 3406 3407 4223 3406 Size: 1 816 6570 Fragments arranged by size: 6570 816 1 EcoRI G'AATT_C Cuts at: 2214 4696 5074 2214 Size: 2482 378 4527 Fragments arranged by size: 4527 2482 378 EcoRV GAT'ATC Cuts at: 2584 2584 Size: 7387 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2123 3491 2123 Size: 1368 6019 FspI TGC'GCA Cuts at: 5893 5893 Size: 7387 HaeII r_GCGC'y Cuts at: 1678 6534 1678 Size: 4856 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2730 2762 3596 3628 3739 Size: 32 135 32 1158 32 834 32 111 Cuts at: 3739 3771 6028 6060 6102 6134 1373 Size: 32 2257 32 42 32 2626 Fragments arranged by size: 2626 2257 1158 834 135 111 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3902 3984 4731 4977 669 Size: 988 281 1964 82 747 246 3079 Fragments arranged by size: 3079 1964 988 747 281 246 82 HindIII A'AGCT_T Cuts at: 748 748 Size: 7387 HpaI GTT'AAC Cuts at: 3984 4977 3984 Size: 993 6394 KpnI G_GTAC'C Cuts at: 4712 4712 Size: 7387 MfeI C'AATT_G Cuts at: 4986 4986 Size: 7387 MluI A'CGCG_T Cuts at: 7263 7263 Size: 7387 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4299 4401 6501 6685 1305 Size: 2994 102 2100 184 2007 Fragments arranged by size: 2994 2100 2007 184 102 MscI TGG'CCA Cuts at: 10 64 1489 1832 3465 4317 10 Size: 54 1425 343 1633 852 3080 Fragments arranged by size: 3080 1633 1425 852 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3705 4018 4292 5345 5704 5863 518 Size: 648 2539 313 274 1053 359 159 2042 Fragments arranged by size: 2539 2042 1053 648 359 313 274 159 MspA1I CmG'CkG Cuts at: 1606 3461 4803 5480 6628 6873 1606 Size: 1855 1342 677 1148 245 2120 Fragments arranged by size: 2120 1855 1342 1148 677 245 NaeI GCC'GGC Cuts at: 4582 4582 Size: 7387 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5896 Fragments arranged by size: 5896 939 552 NdeI CA'TA_TG Cuts at: 387 3978 387 Size: 3591 3796 NgoMIV G'CCGG_C Cuts at: 4580 4580 Size: 7387 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7387 NotI GC'GGCC_GC Cuts at: 4834 4834 Size: 7387 NsiI A_TGCA'T Cuts at: 4679 4679 Size: 7387 NspI r_CATG'y Cuts at: 1132 1229 4137 4297 4677 4745 6290 1132 Size: 97 2908 160 380 68 1545 2229 Fragments arranged by size: 2908 2229 1545 380 160 97 68 PasI CC'CwG_GG Cuts at: 1348 2027 1348 Size: 679 6708 PciI A'CATG_T Cuts at: 4133 6286 4133 Size: 2153 5234 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7387 PmeI GTTT'AAAC Cuts at: 4691 4691 Size: 7387 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5456 5488 6969 7001 5456 Size: 32 1481 32 5842 Fragments arranged by size: 5842 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4223 4223 Size: 7387 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7387 PsiI TTA'TAA Cuts at: 3921 4957 3921 Size: 1036 6351 PspOMI G'GGCC_C Cuts at: 3406 3406 Size: 7387 PstI C_TGCA'G Cuts at: 830 2656 4601 4739 830 Size: 1826 1945 138 3478 Fragments arranged by size: 3478 1945 1826 138 PvuI CG_AT'CG Cuts at: 2001 4606 2001 Size: 2605 4782 PvuII CAG'CTG Cuts at: 3461 3461 Size: 7387 SacI G_AGCT'C Cuts at: 3347 3347 Size: 7387 SalI G'TCGA_C Cuts at: 1936 4729 1936 Size: 2793 4594 SbfI CC_TGCA'GG Cuts at: 4739 4739 Size: 7387 ScaI AGT'ACT Cuts at: 3600 5635 7347 3600 Size: 2035 1712 3640 Fragments arranged by size: 3640 2035 1712 SfcI C'TryA_G Cuts at: 826 1045 2652 3789 4597 4735 5870 6551 6742 Size: 219 1607 1137 808 138 1135 681 191 Cuts at: 6742 826 Size: 1471 Fragments arranged by size: 1607 1471 1137 1135 808 681 219 191 138 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7387 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7387 SmaI CCC'GGG Cuts at: 1872 4714 1872 Size: 2842 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2171 2390 3035 3520 3583 5491 Size: 197 926 228 219 645 485 63 1908 Cuts at: 5491 6392 6654 820 Size: 901 262 1553 Fragments arranged by size: 1908 1553 926 901 645 485 262 228 219 197 63 SnaBI TAC'GTA Cuts at: 493 5178 493 Size: 4685 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7387 SphI G_CATG'C Cuts at: 1132 4745 1132 Size: 3613 3774 SspI AAT'ATT Cuts at: 5 52 3643 4252 5311 5 Size: 47 3591 609 1059 2081 Fragments arranged by size: 3591 2081 1059 609 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7387 StyI C'CwwG_G Cuts at: 513 1065 2004 3475 4401 4826 513 Size: 552 939 1471 926 425 3074 Fragments arranged by size: 3074 1471 939 926 552 425 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2910 2975 4487 5414 5431 5584 5769 Size: 1255 1330 65 1512 927 17 153 185 Cuts at: 5769 6214 325 Size: 445 1498 Fragments arranged by size: 1512 1498 1330 1255 927 445 185 153 65 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2332 2591 3598 Size: 274 80 33 51 1443 355 259 1007 Cuts at: 3598 4040 5633 7345 96 Size: 442 1593 1712 138 Fragments arranged by size: 1712 1593 1443 1007 442 355 274 259 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2769 2801 7347 7379 2769 Size: 32 4546 32 2777 Fragments arranged by size: 4546 2777 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7387 XbaI T'CTAG_A Cuts at: 2218 4633 4723 2218 Size: 2415 90 4882 Fragments arranged by size: 4882 2415 90 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3473 4481 3473 Size: 1008 6379 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7387 XmaI C'CCGG_G Cuts at: 1870 4712 1870 Size: 2842 4545 XmnI GAAnn'nnTTC Cuts at: 5516 5516 Size: 7387 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5195 276 Size: 53 83 186 936 3661 2468 Fragments arranged by size: 3661 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI Bsu36I EcoNI FseI FspAI KasI NarI NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI