(Circular) (Six-base) MAPSORT of: pFN21AB7695.seq Check: 7121 from: 1 to: 5866 pFN21AB7695 5866 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3676 278 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3209 1937 Size: 1272 4594 Acc65I G'GTAC_C Cuts at: 3187 3187 Size: 5866 AclI AA'CG_TT Cuts at: 1563 3993 4366 1563 Size: 2430 373 3063 Fragments arranged by size: 3063 2430 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2339 2420 3152 3929 5313 5581 1632 Size: 707 81 732 777 1384 268 1917 Fragments arranged by size: 1917 1384 777 732 707 268 81 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5669 AflIII A'CryG_T Cuts at: 1203 2217 3057 4765 5742 1203 Size: 1014 840 1708 977 1327 Fragments arranged by size: 1708 1327 1014 977 840 AgeI A'CCGG_T Cuts at: 4697 4697 Size: 5866 AhdI GACnn_n'nnGTC Cuts at: 2859 4595 2859 Size: 1736 4130 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5866 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5866 AlwNI CAG_nnn'CTG Cuts at: 2286 2443 3025 3609 5181 2286 Size: 157 582 584 1572 2971 Fragments arranged by size: 2971 1572 584 582 157 ApaLI G'TGCA_C Cuts at: 3923 5079 3923 Size: 1156 4710 ApoI r'AATT_y Cuts at: 1493 3175 3401 3553 5662 1493 Size: 1682 226 152 2109 1697 Fragments arranged by size: 2109 1697 1682 226 152 AseI AT'TA_AT Cuts at: 160 1032 4420 160 Size: 872 3388 1606 Fragments arranged by size: 3388 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 AvaI C'yCGr_G Cuts at: 1870 1943 2203 2354 2462 2864 3191 1870 Size: 73 260 151 108 402 327 4545 Fragments arranged by size: 4545 402 327 260 151 108 73 AvrII C'CTAG_G Cuts at: 2035 2035 Size: 5866 BamHI G'GATC_C Cuts at: 3196 3563 3196 Size: 367 5499 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2791 3126 3187 4642 618 Size: 325 217 627 1004 335 61 1455 1842 Fragments arranged by size: 1842 1455 1004 627 335 325 217 61 BanII G_rGCy'C Cuts at: 1391 2634 2846 3090 3185 1391 Size: 1243 212 244 95 4072 Fragments arranged by size: 4072 1243 244 212 95 BbeI G_GCGC'C Cuts at: 2795 2795 Size: 5866 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2183 2478 2858 928 Size: 971 284 295 380 3936 Fragments arranged by size: 3936 971 380 295 284 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4057 4091 1903 Size: 34 2120 34 3678 Fragments arranged by size: 3678 2120 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3758 4968 3758 Size: 1210 4656 BclI T'GATC_A Cuts at: 1548 1548 Size: 5866 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2789 2841 4477 Size: 107 122 71 631 605 1117 52 1636 Cuts at: 4477 136 Size: 1525 Fragments arranged by size: 1636 1525 1117 631 605 122 107 71 52 BglII A'GATC_T Cuts at: 5861 5861 Size: 5866 BlpI GC'TnA_GC Cuts at: 2633 3283 2633 Size: 650 5216 Bme1580I G_kGCm'C Cuts at: 1790 3927 5083 1790 Size: 2137 1156 2573 Fragments arranged by size: 2573 2137 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5866 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3597 4555 442 Size: 416 53 83 399 24 2180 958 1753 Fragments arranged by size: 2180 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2285 2465 2588 3124 4526 1239 Size: 1046 180 123 536 1402 2579 Fragments arranged by size: 2579 1402 1046 536 180 123 Bpu10I CC'TnA_GC Cuts at: 2030 2030 Size: 5866 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3991 4856 5154 3991 Size: 865 298 4703 Fragments arranged by size: 4703 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2405 2543 4529 882 Size: 617 906 138 1986 2219 Fragments arranged by size: 2219 1986 906 617 138 BsaAI yAC'GTr Cuts at: 493 1204 2220 3657 493 Size: 711 1016 1437 2702 Fragments arranged by size: 2702 1437 1016 711 BsaBI GATnn'nnATC Cuts at: 5860 5860 Size: 5866 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2792 3673 4055 4690 Size: 53 83 186 936 1259 881 382 635 Cuts at: 4690 275 Size: 1451 Fragments arranged by size: 1451 1259 936 881 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2541 2712 4299 4697 4971 5118 5529 2541 Size: 171 1587 398 274 147 411 2878 Fragments arranged by size: 2878 1587 411 398 274 171 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2861 2891 1178 Size: 30 165 30 1458 30 4153 Fragments arranged by size: 4153 1458 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2420 2423 2681 2684 2693 1186 Size: 1234 3 258 3 9 4359 Fragments arranged by size: 4359 1234 258 9 3 3 BseYI C'CCAG_C Cuts at: 5069 5565 5069 Size: 496 5370 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2391 1163 Size: 573 655 4638 Fragments arranged by size: 4638 655 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2627 3046 3316 4077 5105 1540 Size: 275 186 626 419 270 761 1028 2301 Fragments arranged by size: 2301 1028 761 626 419 275 270 186 BsiHKAI G_wGCw'C Cuts at: 2070 3927 4012 5083 2070 Size: 1857 85 1071 2853 Fragments arranged by size: 2853 1857 1071 85 BsmI GAATG_Cn' Cuts at: 3377 3470 3377 Size: 93 5773 BsmBI CGTCTCn'nnnn_ Cuts at: 2369 2369 Size: 5866 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2634 2846 3090 3185 3927 4012 Size: 399 280 564 212 244 95 742 85 Cuts at: 4012 5083 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 564 399 280 244 212 95 85 BspEI T'CCGG_A Cuts at: 2541 5529 2541 Size: 2988 2878 BspHI T'CATG_A Cuts at: 3755 5485 3755 Size: 1730 4136 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3221 844 Size: 2377 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3753 1394 Size: 429 1930 3507 Fragments arranged by size: 3507 1930 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4361 4535 65 Size: 1183 3113 174 1396 Fragments arranged by size: 3113 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2138 2347 2622 4510 4697 1700 Size: 110 328 209 275 1888 187 2869 Fragments arranged by size: 2869 1888 328 275 209 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5866 BssHII G'CGCG_C Cuts at: 1925 2087 1925 Size: 162 5704 BssSI C'ACGA_G Cuts at: 2911 3926 4938 2911 Size: 1015 1012 3839 Fragments arranged by size: 3839 1015 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2976 2985 2994 794 Size: 2182 9 9 3666 Fragments arranged by size: 3666 2182 9 9 BstBI TT'CG_AA Cuts at: 4670 4670 Size: 5866 BstEII G'GTnAC_C Cuts at: 1184 3050 1184 Size: 1866 4000 BstYI r'GATC_y Cuts at: 1972 3196 3563 3948 3965 5417 5503 5515 5861 Size: 1224 367 385 17 1452 86 12 346 Cuts at: 5861 1972 Size: 1977 Fragments arranged by size: 1977 1452 1224 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2760 2760 Size: 5866 BtgI C'CryG_G Cuts at: 513 1065 2004 2185 3078 513 Size: 552 939 181 893 3301 Fragments arranged by size: 3301 939 893 552 181 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5127 Fragments arranged by size: 5127 683 56 BtsI GCAGTG_nn' Cuts at: 2068 2972 3382 4176 4196 2068 Size: 904 410 794 20 3738 Fragments arranged by size: 3738 904 794 410 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5866 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5831 DraI TTT'AAA Cuts at: 3170 3170 Size: 5866 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2804 4873 809 Size: 507 1488 2069 1802 Fragments arranged by size: 2069 1802 1488 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2624 2661 Size: 54 1425 50 275 18 177 617 37 Cuts at: 2661 3313 4202 5831 8 Size: 652 889 1629 43 Fragments arranged by size: 1629 1425 889 652 617 275 177 54 50 43 37 18 EagI C'GGCC_G Cuts at: 1537 1812 2624 3313 1537 Size: 275 812 689 4090 Fragments arranged by size: 4090 812 689 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3796 1353 Size: 2443 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3098 4453 4827 4973 387 Size: 1064 138 1509 1355 374 146 1280 Fragments arranged by size: 1509 1355 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 2339 2420 2465 2588 3124 3152 Size: 393 653 54 81 45 123 536 28 Cuts at: 3152 3929 4526 5313 5581 1239 Size: 777 597 787 268 1524 Fragments arranged by size: 1524 787 777 653 597 536 393 268 123 81 54 45 28 EcoNI CCTnn'n_nnAGG Cuts at: 2039 2321 2663 2039 Size: 282 342 5242 Fragments arranged by size: 5242 342 282 EcoO109I rG'GnC_Cy Cuts at: 2269 2283 2755 2869 2269 Size: 14 472 114 5266 Fragments arranged by size: 5266 472 114 14 EcoRI G'AATT_C Cuts at: 3175 3553 3175 Size: 378 5488 EcoRV GAT'ATC Cuts at: 2599 2599 Size: 5866 FspI TGC'GCA Cuts at: 4372 4372 Size: 5866 HaeII r_GCGC'y Cuts at: 1678 2795 5013 1678 Size: 1117 2218 2531 Fragments arranged by size: 2531 2218 1117 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2244 2276 2845 2861 2877 Size: 32 135 32 672 32 569 16 16 Cuts at: 2877 2893 2966 2998 4507 4539 4581 4613 1373 Size: 16 73 32 1509 32 42 32 2626 Fragments arranged by size: 2626 1509 672 569 135 73 42 32 32 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 3210 3456 669 Size: 988 281 1272 246 3079 Fragments arranged by size: 3079 1272 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5866 HpaI GTT'AAC Cuts at: 3456 3456 Size: 5866 KasI G'GCGC_C Cuts at: 2791 2791 Size: 5866 KpnI G_GTAC'C Cuts at: 3191 3191 Size: 5866 MfeI C'AATT_G Cuts at: 3465 3465 Size: 5866 MluI A'CGCG_T Cuts at: 5742 5742 Size: 5866 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2216 4980 5164 1305 Size: 911 2764 184 2007 Fragments arranged by size: 2764 2007 911 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3867 Fragments arranged by size: 3867 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2168 3824 4183 4342 518 Size: 648 1002 1656 359 159 2042 Fragments arranged by size: 2042 1656 1002 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2187 2232 2961 3022 3282 3959 5107 5352 Size: 581 45 729 61 260 677 1148 245 Cuts at: 5352 1606 Size: 2120 Fragments arranged by size: 2120 1148 729 677 581 260 245 61 45 NarI GG'CG_CC Cuts at: 2792 2792 Size: 5866 NcoI C'CATG_G Cuts at: 513 1065 2004 3078 513 Size: 552 939 1074 3301 Fragments arranged by size: 3301 1074 939 552 NdeI CA'TA_TG Cuts at: 387 2524 387 Size: 2137 3729 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5866 NotI GC'GGCC_GC Cuts at: 3313 3313 Size: 5866 NspI r_CATG'y Cuts at: 1132 1229 3061 3224 4769 1132 Size: 97 1832 163 1545 2229 Fragments arranged by size: 2229 1832 1545 163 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5866 PciI A'CATG_T Cuts at: 3057 4765 3057 Size: 1708 4158 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5866 PfoI T'CCnGG_A Cuts at: 2262 2337 2262 Size: 75 5791 PmeI GTTT'AAAC Cuts at: 3170 3170 Size: 5866 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3935 3967 5448 5480 3935 Size: 32 1481 32 4321 Fragments arranged by size: 4321 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2269 2755 2869 2269 Size: 486 114 5266 Fragments arranged by size: 5266 486 114 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5866 PsiI TTA'TAA Cuts at: 3436 3436 Size: 5866 PspXI vC'TCGA_Gb Cuts at: 2354 2462 2354 Size: 108 5758 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2144 2176 2144 Size: 32 5834 PstI C_TGCA'G Cuts at: 830 2990 3218 830 Size: 2160 228 3478 Fragments arranged by size: 3478 2160 228 PvuI CG_AT'CG Cuts at: 2001 3046 2001 Size: 1045 4821 PvuII CAG'CTG Cuts at: 3022 3022 Size: 5866 SacII CC_GC'GG Cuts at: 2188 2188 Size: 5866 SalI G'TCGA_C Cuts at: 1936 3208 1936 Size: 1272 4594 SanDI GG'GwC_CC Cuts at: 2755 2755 Size: 5866 SbfI CC_TGCA'GG Cuts at: 3218 3218 Size: 5866 ScaI AGT'ACT Cuts at: 4114 5826 4114 Size: 1712 4154 SfcI C'TryA_G Cuts at: 826 1045 2986 3214 4349 5030 5221 826 Size: 219 1941 228 1135 681 191 1471 Fragments arranged by size: 1941 1471 1135 681 228 219 191 SfoI GGC'GCC Cuts at: 2793 2793 Size: 5866 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5866 SmaI CCC'GGG Cuts at: 1872 2205 3193 1872 Size: 333 988 4545 Fragments arranged by size: 4545 988 333 SmlI C'TyrA_G Cuts at: 820 1017 1943 2354 2462 3970 4871 5133 820 Size: 197 926 411 108 1508 901 262 1553 Fragments arranged by size: 1553 1508 926 901 411 262 197 108 SnaBI TAC'GTA Cuts at: 493 3657 493 Size: 3164 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5866 SphI G_CATG'C Cuts at: 1132 3224 1132 Size: 2092 3774 SspI AAT'ATT Cuts at: 5 52 3790 5 Size: 47 3738 2081 Fragments arranged by size: 3738 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5866 StyI C'CwwG_G Cuts at: 513 1065 2004 2035 3078 3305 513 Size: 552 939 31 1043 227 3074 Fragments arranged by size: 3074 1043 939 552 227 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2589 2663 2669 3893 3910 4063 4248 Size: 1255 1009 74 6 1224 17 153 185 Cuts at: 4248 4693 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1224 1009 445 185 153 74 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2292 3060 4112 Size: 274 80 33 51 1443 315 768 1052 Cuts at: 4112 5824 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1052 768 315 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5826 5858 5826 Size: 32 5834 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5866 XbaI T'CTAG_A Cuts at: 3202 3202 Size: 5866 XhoI C'TCGA_G Cuts at: 1943 2354 2462 1943 Size: 411 108 5347 Fragments arranged by size: 5347 411 108 XmaI C'CCGG_G Cuts at: 1870 2203 3191 1870 Size: 333 988 4545 Fragments arranged by size: 4545 988 333 XmnI GAAnn'nnTTC Cuts at: 3995 3995 Size: 5866 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3674 276 Size: 53 83 186 936 2140 2468 Fragments arranged by size: 2468 2140 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI RsrII SacI SapI SexAI SfiI SrfI SwaI XcmI