Restriction map for
pFN21AB6496
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbsI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaHI BsaI BseAI
BsiWI BsmFI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstBI BstEII BstPI
BstUI Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HindIII KasI
KpnI Ksp632I KspI MluI MluNI
MroI MscI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SphI Sse8387I SwaI
Tth111I XhoI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 1051 GTATAC GT^[AC][GT]AC
ApaI 540 GGGCCC GGGCC^C
AvaI 863 CCCGGG C^[CT]CG[GA]G
AvaII 527 GGACC G^G[AT]CC
BanI 93 GGTGCC G^G[CT][GA]CC
BbrPI 691 CACGTG CAC^GTG
BpmI 707 CTGGAG CTGGAG(16/14)
BsaAI 691 CACGTG [CT]AC^GT[GA]
BsaBI 286 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 164 ACCGGA [AT]^CCGG[AT]
BseRI 761 GAGGAG GAGGAG(10/8)
BsgI 1042 GTGCAG GTGCAG(16/14)
BsiEI 162 CGACCG CG[GA][CT]^CG
BsmBI 650 GAGACG CGTCTC(1/5)
BsmI 583 GAATGC GAATGC(1/-1)
BsoBI 863 CCCGGG C^[CT]CG[GA]G
Bsp1407I 773 TGTACA T^GTACA
BsrGI 773 TGTACA T^GTACA
BsrI 807 ACTGG ACTGG(1/-1)
Bst1107I 1051 GTATAC GTA^TAC
BstXI 530 CCACCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1051 GTATAC GTA^TAC
Cac8I 483 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 549 GCGC GCG^C
DdeI 16 CTGAG C^T[ACGT]AG
DraII 540 GGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 303 CCATGG C^C[GA][CT]GG
EcoO109I 540 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 303 CCATGG C^C[AT][AT]GG
HaeII 548 GGCGCT [GA]GCGC^[CT]
HhaI 549 GCGC GCG^C
HinP1I 549 GCGC G^CGC
HincII 990 GTTAAC GT[CT]^[GA]AC
HindII 990 GTTAAC GT[CT]^[GA]AC
HpaI 990 GTTAAC GTT^AAC
MaeII 692 ACGT A^CGT
MamI 286 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 507 CAATTG C^AATTG
MspA1I 535 CAGCTG C[AC]G^C[GT]G
MunI 507 CAATTG C^AATTG
NcoI 303 CCATGG C^CATGG
PleI 802 GAGTC GAGTC(4/5)
PmaCI 691 CACGTG CAC^GTG
PmlI 691 CACGTG CAC^GTG
PstI 148 CTGCAG CTGCA^G
PvuII 535 CAGCTG CAG^CTG
SacI 297 GAGCTC GAGCT^C
SfaNI 522 GATGC GCATC(5/9)
SmaI 863 CCCGGG CCC^GGG
SpeI 600 ACTAGT A^CTAGT
SspBI 773 TGTACA T^GTACA
StuI 128 AGGCCT AGG^CCT
StyI 303 CCATGG C^C[AT][AT]GG
TaqI 99 TCGA T^CGA
TfiI 725 GAATC G^A[AT]TC
TthHB8I 99 TCGA T^CGA
XbaI 411 TCTAGA T^CTAGA
XcmI 534 CCAGCTGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 863 CCCGGG C^CCGGG
XmaI 863 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcsI 196 AAATTC [GA]^AATT[CT]
444 AAATTT
AlwI 178 GATCC GGATC(4/5)
204 GGATC
ApoI 196 AAATTC [GA]^AATT[CT]
444 AAATTT
AspHI 18 GAGCAC G[AT]GC[AT]^C
297 GAGCTC
BcnI 863 CCCGG CC^[GC]GG
864 CCGGG
BglII 83 AGATCT A^GATCT
408 AGATCT
BsiHKAI 18 GAGCAC G[AT]GC[AT]^C
297 GAGCTC
BstAPI 20 GCACCTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
516 GCAAGAGATGC
HinfI 725 GAATC G^A[ACGT]TC
802 GAGTC
NciI 863 CCCGG CC^[GC]GG
864 CCGGG
PflMI 201 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
564 CCAGGAGGTGG
SfcI 132 CTATAG C^T[GA][CT]AG
148 CTGCAG
SspI 379 AATATT AAT^ATT
747 AATATT
TspRI 806 CACTG CA[GC]TG[ACGT][ACGT]^
1040 CAGTG
Van91I 201 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
564 CCAGGAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AfaI AluI AlwNI BanII
BbvI BfaI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI BstYI Cfr13I DpnII EcoRII
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
Tru9I TseI Tsp45I Tsp509I XhoII